Immune System Of Rainbow Trout Research Articles

Immunocompetence and alterations in hepatic gene expression in rainbow trout exposed to CdS/CdTe quantum dots

Cadmium (Cd) tellurium quantum dots (QDs) are long-lived fluorescent nanocrystals that have the ability to produce potentially toxic reactive oxygen species at the surface of the nanoparticle and release toxic cadmium ions. The purpose of this study was to examine the sublethal effects of CdS/CdTe QDs and dissolved Cd on the immune system of rainbow trout. Changes in hepatic gene expression were also monitored to provide insight on the mode of action of both forms of cadmium. Oncorhynchus mykiss fish were exposed to increasing concentrations of CdS/CdTe or dissolved Cd (CdSO(4)) for 48 h at 15 degrees C. The anterior head kidney was analyzed for leukocyte count, viability and phagocytic activity. The livers were harvested and prepared for gene expression analysis using a DNA microarray comprised of 207 stress-related genes. An analysis of total Cd in the aquarium water revealed that the nominal concentrations corresponded well to the actual concentrations and that a small proportion (0.4%) of Cd in the QDs was found in the dissolved fraction. Exposure to QDs led to significant reductions in leukocyte counts, viability and both resting and active phagocytic activity. On a mass concentration basis, QDs were more potent than dissolved Cd in suppressing immunocompetence in rainbow trout. The analysis revealed that both forms of Cd were strong inducers of metallothionein and CP2K1 gene expressions, which are respectively involved in metal detoxification processes and xenobiotic transformation/inflammation conditions. The analysis revealed different modes of action for each form of Cd. For QDs, 25 genes specific to QDs and related to the immune endpoints were found. The genes were involved in inflammation, xenobiotic biotransformation and endocrine system (including the induction of vitellogenin and its receptor). The effect for dissolved Cd was narrower than for QDs, with 9 genes specific to dissolved Cd being strongly correlated with the observed effects on immunocompetence. The involved genes were binding and transport of various solutes (urea, sodium, potassium) and the complement system. The present study revealed that each form of Cd produced a different pattern of gene expression and lowered fish immunocompetence.

Immunotoxicological effects of an activated-sludge-treated effluent on rainbow trout ( Oncorhynchus mykiss)

Municipal effluents are often treated by biological processes designed to enhance natural biodegradation that introduce important changes to the local bacterial community and contaminant status. The purpose of this study was to examine the immunotoxicological effects of an activated sludge treated urban effluent on rainbow trout ( Oncorhynchus mykiss). Trout specimens were exposed to increasing concentrations of the effluent at 0.1%, 1% and 10% v/v dilutions for 30 days at 15 °C. After the exposure period, the leukocytes were isolated from the anterior kidney and the following parameters were determined: phagocytic activity, lymphoblastic transformation and natural cytotoxic activity. The results revealed that phagocytic activity was not significantly affected by the biologically treated effluent. Natural cytotoxic activity was significantly increased at an effluent threshold concentration of 3% v/v at an effector/target cell ratio of 20:1 and 40:1. Stimulated lymphocyte T and B proliferation was significantly decreased at a threshold concentration of 0.3%. However, neither the unstimulated lymphocyte T nor lymphocyte B proliferation was significantly affected by effluent exposure. The results suggest that the activated-sludge-treated effluent increased cell-mediated cytotoxic activity with a concomitant decrease in cells responsible for antibody and cytokine production, indicating that the immune system of rainbow trout was directed towards the elimination of transformed cells, virus-infected cells and protozoan parasites at the expense of cytokine and antibody production.

Impact of estrogenic active substances on the developing immune system of rainbow trout (Oncorhynchus mykiss)

Impact of estrogenic active substances on the developing immune system of rainbow trout (Oncorhynchus mykiss)

The effects of a primary-treated municipal effluent on the immune system of rainbow trout ( Oncorhynchus mykiss): Exposure duration and contribution of suspended particles

Municipal sewage effluents are complex mixtures of contaminants known to disrupt both immune and endocrine functions in aquatic organisms. The present study sought to determine the impacts of municipal effluent on the immune systems of juvenile rainbow trout ( Oncorhynchus mykiss), by exposing specimens to low concentrations (0.01%, 0.1%, 1% or 10%) of sewage effluent for periods of 28 or 90 days. The soluble and insoluble fractions of the effluent were also studied to assess the contribution of fractions rich in microorganisms and particles on fish immune systems. To this end, the trout were also exposed to soluble and insoluble fractions of the effluent for a period of 28 days. Immunocompetence was assessed by the following three parameters: phagocytosis, natural cytotoxic cells (NCC) and blastogenesis of lymphocytes under mitogen stimulation. Fish exposed to the 1% sewage effluent concentration for 28 days had enhanced phagocytic activity; at 90 days, phagocytic activity was reduced. T and B lymphocyte proliferation in fish from both groups was similarly stimulated. Phagocytosis and NCC activities were influenced more by the insoluble fraction than the soluble fraction of the effluent. Conversely, mitogen-stimulated T lymphocyte proliferation was enhanced in cells of fish exposed to the soluble fraction of the effluents, with a dampening effect on the insoluble (particulate) fraction of the effluent. In conclusion, the effects of the effluent and its fractions were higher at the cellular-mediated immunity level than at the acquired immunity level. Immunotoxicity data on the soluble fraction of the effluent were more closely associated to data on the unfractionated effluent, but the contribution of the particulate fraction could not be completely ignored for phagocytosis and B lymphocyte proliferation.

Effects of ozone, ultraviolet and peracetic acid disinfection of a primary-treated municipal effluent on the Immune system of rainbow trout ( Oncorhynchus mykiss)

Municipal sewage effluents are complex mixtures that are known to compromise the health condition of aquatic organisms. The aim of this study was to evaluate the impacts of various wastewater disinfection processes on the immune system of juvenile rainbow trout ( Oncorhynchus mykiss). The trout were exposed to a primary-treated effluent for 28 days before and after one of each of the following treatments: ultraviolet (UV) radiation, ozonation and peracetic acid. Immune function was characterized in leucocytes from the anterior head kidney by the following three parameters: phagocytosis activity, natural cytotoxic cells (NCC) function and lymphocyte (B and T) proliferation assays. The results show that the fish mass to length ratio was significantly decreased for the primary-treated and all three disinfection processes. Exposure to the primary-treated effluent led to a significant increase in macrophage-related phagocytosis; the addition of a disinfection step was effective in removing this effect. Both unstimulated and mitogen-stimulated T lymphocyte proliferation in fish decreased dramatically in fish exposed to the ozonated effluent compared to fish exposed to either the primary-treated effluent or to aquarium water. Stimulation of T lymphocytes proliferation was observed with the peracetic acid treatment group. In conclusion, the disinfection strategy used can modify the immune system in fish at the level of T lymphocyte proliferation but was effective to remove the effects on phagocytosis activity.

A new putative G-protein coupled receptor gene associated with the immune system of rainbow trout ( Oncorhynchus mykiss)

A putative G-protein coupled receptor (GPCR) gene belonging to the rhodopsin-like family was detected in rainbow trout and designated LPSenhR-1. Only moderate homology (<35%) was present with known GPCRs. Semi-quantitative RT-PCR indicated that the gene was expressed predominantly in lymphoid tissues, with highest expression associated with cells of the monocyte/macrophage lineage. Expression was strongly up-regulated by injection with LPS but not by infection with infectious haematopoietic necrosis virus.

Dietary vitamin E and rainbow trout (Oncorhynchus mykiss) phagocyte functions: effect on gut and on head kidney leucocytes

The effects of vitamin E (deficiency or supplementation) on the non-specific immune system in rainbow trout, Oncorhynchus mykiss, were evaluated. Rainbow trout were fed daily a semi-purified diet supplemented with vitamin E at 0, 28 and 295mg kg−1of diet. After 80 days of experimental feeding, the phagocytic function (respiratory burst evaluated by the CL response, phagocytosis) from gut leucocytes and head kidney enriched macrophages was measured; head kidney cell pinocytosis and serum lysozyme activity were also analysed. The results showed that some phagocyte functions were influenced by dietary vitamin E. When fish were fed the high dietary dose of vitamin E an enhancement of phagocytosis was found, but only significantly for the leucocytes isolated from the gut of rainbow trout; moreover, an impaired response was also observed in the fish fed no vitamin E for 80 days. However, no significant differences were noticed on the oxidative burst (CL) response of both gut and head kidney cells according to the dietary dose of vitamin E. Pinocytosis evaluated on head kidney cells was not influenced by dietary vitamin E. Fish fed vitamin E at 295mg kg−1had a lower serum lysozyme activity than those fed with vitamin E at 28mg kg−1and the fish fed no vitamin E for 80 days had an impaired activity. Thus, the present results demonstrate that altered dietary levels of vitamin E modulates the phagocytic functions of gut leucocytes in rainbow trout; moreover, the vitamin E diet effect seems to be greater on the local intestinal response as compared to systemic (head kidney). Taken together, this study confirms the crucial role of gut phagocytes in mucosal non-lymphoid defences in fish.

LP1 The role of inducible nitric oxide synthase and nitric oxide in the immune system of rainbow trout

LP1 The role of inducible nitric oxide synthase and nitric oxide in the immune system of rainbow trout

KP1 The role of inducible nitric oxide synthase and nitric oxide in the immune system of rainbow trout

KP1 The role of inducible nitric oxide synthase and nitric oxide in the immune system of rainbow trout

Comparisons of nonspecific and specific immunomodulation by oxolinic acid, oxytetracycline and levamisole in salmonids

Oxolinic acid, a promising drug for the treatment of bacterial fish disease agents, was tested for possible immunomodulatory effects on fish. Another antibiotic oxytetracycline, known to be immunosuppressive at higher treatment doses, and levamisole, a known immunostimulator for higher vertebrates, were also compared for causing changes in the nonspecific defense compartment and the specific immune system in rainbow trout. Groups of fish were immunized with Yersinia ruckeri O-antigen bacterin in combination with selected doses of the drugs. The nonspecific defense activity was measured by demonstrating neutrophil metabolic activity by the nitroblue tetrazolium assay, by counting engulfed bacterial cells for a phagocytic index and by counting leukocytes with adherent bacterial cells for the adherence index. The specific immune response was monitored by the passive hemolytic plaque assay demonstrating the numbers of antibody-producing cells. The results showed that oxolinic acid, used at recommended doses for the treatment of bacterial diseases, did not cause immunosuppression in either the nonspecific defense or specific immune system compartments, whereas tetracycline at 10 mg/kg caused reduced activity in both. Fish given levamisole injections before the antigen injection showed a stimulated nonspecific defense but a much reduced specific immune response.

Immunological roles of sensitive lymphocytes and resistant lymphocytes against cyclophosphamide in rainbow trout immune system reorganized by adoptive transfer.

The immune systems of fish are still unclear and moreover, a cell-to-cell cooperation, which is a basic immune mechanism in birds, murines and humans, is relatively unknown in fish immunity. Adoptive transfer, which is one of the most valuable and practical approaches for tracing roles of immunocompetent cells, was applied in order to understand the immune system of rainbow trout. Cyclophosphamide and mitomycin C were tested to determine a competency for immunosuppressive agent against rainbow trout immunized with goldish red blood cells (GFRBC). Cyslophosphamide of an intraperitoneal injection dose of 400-500mg/kg was best suppressible, while mitomycin C of a high dose was acutely toxic but a low dose indicated an unsuppressive function in the titration of antiGFRBC antibody. When lymphocytes isolated from the blood and spleen of GFRBC-primed cyclophosphamide-administered rainbow trout were intravascularly transferred to unprimed rainbow trout, the antibody titers of the recipients became markedly low or undetectable, This suggested that the transfer of cyclophosphamide-resistant cells were ineffective and, therefore, the lack of cyclophosphamide-sensitive cells was fatal to the immune response of the recipients. When lymphocytes including cyclophosphamide-resistant cells and cycophosphamide-sensitive cells, separated from GFRBC-primde donors were transferred to immunological non-responders as recipients (which were treated with cyclophosphamide), the antibody respomse of the recipients was restored to the same titer levels as in normally immunized trout and normally transferred trout. The restoration in this adoptive transfer is able to support the following tow points: an antibody response of rainbow trout clearly depends upon two lymphocyte subpopulations, cyclophosphamide-sensitive cells and cyclophosphamide-resistant cells;and a cell-to cell cooperation of the two cell lines is indispensable for the production of the antibody.