Immune Function In Animals Research Articles

Evolution and expression analysis of carotenoid cleavage oxygenase gene family in Chinese mitten crab Eriocheir sinensis

Carotenoid cleavage oxygenase (CCO) plays a pivotal role in various biological activities, including antioxidant and immune functions in animals. This paper investigates the evolution and expression of CCO genes based on three chordates and 27 arthropods. Aquatic animals exhibit a higher abundance of CCO genes. Despite this, research on CCO in crustaceans has been notably limited, with a complete absence of any previous studies on the CCO genes for the Chinese mitten crab (Eriocheir sinensis). In this study, six CCO genes were identified in the E. sinensis genome database. Results reveal that the evolution of the CCO gene family in Crustacea is primarily characterized by purifying selection, with a preference for employing similar codons. EsCCO1 and EsCCO3 were mainly expressed in the epidermal layer, and EsCCO4 was mainly expressed in the hindgut. Meanwhile, EsCCO5 and EsCCO6 were mainly expressed in the hepatopancreas and endometrium. A notable detail that different EsCCO genes demonstrate distinct expression patterns within various tissues of E. sinensis. The findings of this study offer fundamental insights that could serve as a basis for further exploration into the functions and regulatory mechanisms of CCO genes in crustacean species.

Effects of Yu-Ping-Feng polysaccharides on animal growth performance and immune function: a review.

Yu-Ping-Feng polysaccharides (YPF-P) is the primary component of Yu-Ping-Feng San (YPF-S) medicine prescription, which consists of three polysaccharides from Astragalus Membranaceus polysaccharide (AM-P), Atractylodes Macrocephala polysaccharide (AM-P), and Saposhnikovia Divaricata polysaccharide (SD-P). The use of Yu-Ping-Feng polysaccharides dates back to the Yuan Dynasty when Yilin Wei first utilized it. The remedy is included in "Effective Formulae Handed Down for Generations" and "The Pharmacopoeia of the People's Republic of China." Yu-Ping-Feng polysaccharides is known to promote growth and enhance the body's immune function in animals. As such, it has promising application prospects in animal husbandry. This review mainly introduces the main components and characteristics of Yu-Ping-Feng polysaccharides, its effects on animal production, and its impact on animal immune function. Additionally, this paper offers a preliminary discussion on the development and utilization of Yu-Ping-Feng polysaccharides, laying the foundation for further research and application. This review may also provide insight and reference for the development of the farming industry, reducing production costs and improving productivity.

Trash to treasure: lactate and protein lactylation in maize root impacts response to drought.

Lactate, protein lactylation (Kla), and specifically histone lactylation have recently been shown to regulate antipathogenic immune responses in mammals. Herein, after we confirmed the presence and accumulation of lactate in maize roots under drought conditions, a lactylome profiling analysis revealed that Kla modifications were invariably present in maize roots, that there were obvious differences in the lactylomes of drought-sensitive (B73) vs. drought-tolerant (Jing2416) lines, and that growing Jing2416 under drought conditions caused significant decreases in the lactylation of multiple enzymes responsible for fatty acid degradation. Inspired by findings of histone-Kla based epigenetic regulation of immune functions in animals, we initially discovered 37 Kla sites on 16 histones in the maize genome, and again detected obvious differential histone Kla-mediated trends between two lines by ChIP-Seq. Notably, only 2.7% of genes with differential histone Kla peaks detected during drought stress were commonly present in both lines, a finding demonstrating that abiotic stress triggers distinct epigenetic activities in diverse germplasm while also strongly supporting that a histone Kla layer of regulation is associated with physiological responses to drought stress. Interestingly, exogenous application of spermidine improved the drought tolerance of B73 and substantially altered the levels of lactate, protein lactylation, and histone Kla modification. Thus, beyond extending the known domain of Kla-based biochemical and epigenetic regulation from animal immunity to plant stress physiology, our study suggests the physiological, biochemical, and genetic function of "the best-known metabolic waste", lactate.

The use of selenomethionine to reduce ammonia toxicity in porcine spleen by inhibiting endoplasmic reticulum stress and autophagy mediated by oxidative stress

Ammonia (NH3) is a typical pollutant in the atmosphere and is well known for its harmful effects on plants, animals as well as human health. Previous studies have shown that NH3 exposure can cause damage to immune organs and impaired immune function in animals. Selenomethionine is a kind of organic selenium, which can not only promote the growth and development of the body, but also inhibit the generation of intracellular reactive oxygen species (ROS), and effectively improve the immune function of the body. Therefore, this study evaluated the toxic effect of NH3 exposure on spleen from a new perspective and investigated the protective effect of selenomethionine on ammonia-induced immunotoxicity. Twenty-four Large White*Duroc*Min pigs were randomly assigned to 4 groups: control group, NH3 group, selenium group, and NH3 + selenium group. Our results showed that NH3 inhalation caused autophagy in the pig spleen, a decrease in lymphocytes, and an increase in autophagic vesicles. Also, NH3 exposure led to a decrease in the activity of some antioxidant enzymes (decreased by about 50%) and a significant increase in the expression of genes related to oxidative stress and endoplasmic reticulum stress (ERS). Our results indicated that selenomethionine mitigated ammonia toxicity in pigs (alleviated about 20–55%). In summary, our findings should be of value in providing a theoretical basis for revealing the toxicity of the high-risk gas NH3, and providing a new perspective on the mechanism of Se against toxic substances.

Cloning of Grass Carp Chemokine XC Receptor 1 (XCR1) Gene and Evaluation of Its Expression in Various Organs after GCRV Infection

Interaction between the chemokine receptor XCR1 and its ligand is closely related to the immune function in animals; however, there are only a few reports on role of XCR1 in the immune system of fish. We aimed to analyze the expression of XCR1 in various organs or tissues of grass carp before and after Grass Carp Reovirus (GCRV) infection to better understand the function of XCR1 in resistance to GCRV infection. We cloned and sequenced the cDNA of grass carp XCR1 and analyzed the molecular structure of XCR1 based its amino acid sequence. Further, we analyzed the relative expression levels of XCR1 in different organs or tissues of male parent grass carp with GCRV resistance (P1) and their first-generation offspring (F1) before and after GCRV infection. Our results show that the total length of cDNA of the grass carp XCR1 gene is 1659 bp and encodes 365 amino acids. XCR1 contains seven conserved transmembrane helical domains. The homologous tertiary structure of XCR1 is similar to its homologs in other species. After artificial GCRV infection, there were significant differences in the expression of the grass carp XCR1 gene in different tissues, at different time points, and between P1 and F1 fish. These results will contribute to our understanding of the role of XCR1 in fish immune responses and contribute to the development of GCRV-resistant grass carp.

The immunotoxicity of decabromodiphenyl ether (BDE-209) on broiler chicks by transcriptome profiling analysis

Decabromodiphenyl ether (BDE-209) has drawn significant attention due to its suppression of immune functions in animals and even humans. In order to explore the mechanism through which BDE-209 affects the immune system, broiler chicks were fed a diet containing various concentrations of BDE-209 (0, 0.004, 0.04, 0.4, and 4 g/kg) for 42 days. Histopathological observations of immune organs found damaged and necrotic lymphocytes in the spleen and bursa, and losses of lymphoid cells in thymic gland. The activities of catalase, glutathione, glutathione peroxidase, and superoxide dismutase in both the spleen and serum were affected by BDE-209. Obvious bioaccumulation effect was found in spleen tissues (high to 1339 ± 181.9 μg/kg). Furthermore, transcriptome sequencing analyses of the spleen identified 424 upregulated and 301 downregulated DEGs, and the cytokine-cytokine receptor interaction signal pathway was most significantly enriched based on the Kyoto Encyclopedia of Genes and Genomes database. Quantitative real-time PCR affirmed the decreased expressions of interleukin IL18, IL18R1, IL18RAP, IL21, as well as interferon gamma IFNG and tumor necrosis factor superfamily members TNFSF8, indicating significant interference to immunomodulation function and possible disease progression in inflammatory effects resulting from BDE-209 exposure. The immunotoxicity of BDE-209 may cause the suppression of immune and physiological functions of spleen cells, leading to inflammation and apoptosis and ultimately spleen atrophy.

Combating Age-Associated Immune Decline Using Kynurenine Pathway Interventions

Select kynurenine pathway interventions extend lifespan in invertebrate models and are of interest in treating age-associated diseases. Kynurenine pathway activity is responsive to inflammatory signaling, and we are evaluating the potential for these interventions to increase pathogen resistance and curtail age-associated immune decline in Caenorhabditis elegans and mammals. The kynurenine pathway facilitates the catabolism of tryptophan to nicotinamide adenine dinucleotide (NAD). Our lab has found that supplementing the kynurenine metabolite 3-hydroxyanthranilic acid (3HAA) or inhibiting the enzyme 3HAA dioxygenase (HAAO) extends lifespan in C. elegans. 3HAA has demonstrated pro/anti-inflammatory properties in mammals, suggesting a potential role in immune function. C. elegans have a primitive immune system that lacks an adaptive element, but it recapitulates aspects of innate immune signaling and pathogen response. I hypothesize kynurenine pathway interventions that impact C. elegans’ lifespan similarly improve pathogen resistance and immunity. Interventions within the kynurenine pathway are capable of differentially impacting pathogenesis and lifespan of C. elegans challenged with Psuedomonas aeruginosa. C. elegans subjected to select lifespan-extending kynurenine pathway interventions fared better when challenged with P. aeruginosa at older ages. Additionally, fluorescent infection tracking has displayed decreased infection rates in worms with elevated 3HAA. Our data suggests pro-immune activity is facilitated by 3HAA acting downstream of the dbl-1 pathway in addition to directly inhibiting bacterial growth. Our goal is to discover the mechanism(s) through which the kynurenine pathway interacts with immune function in animals and identify potential targets for clinical therapy in aging populations.

Effects of Se-Yeast on Immune and Antioxidant in the Se-Deprived Pishan Red Sheep.

The experiments were to study the effects of Se-yeast on immune and antioxidant in Selenium(Se)-deprived Pishan red sheep in Southern Xinjiang, China. The samples of soil, forage, and animal tissues were collected, and used for measuring mineral content, physiological parameter, and biochemical values. These findings showed that the Se contents in affected soil and forage were markedly lower than those from unaffected soil and forage (P < 0.01). Se in affected blood and wool were also extremely lower than those from healthy Pishan red sheep (P < 0.01). The hemoglobin, packed cell volume, platelet count, Glutathione peroxidase, and total antioxidant capacity in the affected Pishan red sheep were markedly lower than those from healthy ones too (P < 0.01). The levels of malondialdehyde, total nitric oxide synthase, and lipid peroxide in Pishan red sheep from affected pastures were extremely higher than those from healthy ones (P < 0.01). The levels of interleukin (IL)-1β, Interleukin-2, tumor necrosis factor-α and interleukin-6 from serum were markedly decreased in affected Pishan red sheep(P < 0.01). The Pishan red sheep in Se-deprived pasture were treated by orally with Se-yeast, the amount of Se in the blood markedly increased in treated animals. Meanwhile, the immune and antioxidant indicator was returned to the healthy values. Consequently, our findings were indicated that Se-deprived forage caused oxidative damage, and a serious threat to the immune function in animals. The Se-yeast is more effective in the Se-deficient Pishan red sheep including blood Se content, immune function and the antioxidant capacity.

Protective Effect of Selenomethionine on T-2 Toxin-Induced Rabbit Immunotoxicity.

T-2 toxin is a trichothecene mycotoxin produced by fusarium species, which is mainly prevalent in grain and livestock feed. One of the main effects of this toxin is immunodepression. Previous studies have shown that T-2 toxin can cause damage to immune organs and impaired immune function in animals. However, selenomethionine (SeMet) as an organic selenium source can not only promote the growth and development of the body but also effectively improve the body's immune function. In this study, rabbits were exposed to 0.4-mg/kg T-2 toxin, and abnormal blood routine indicators were found in the rabbits. HE staining also showed obvious lesions in the spleen and thymus tissue structures, accompanied by a large number of bleeding points. In addition, rabbits showed strong oxidative stress and inflammatory response after T-2 toxin action. 0.2 mg/kg, 0.4 mg/kg, and 0.6 mg/kg organic selenium were added to the feed. However, it was found that 0.2 mg/kg selenium can effectively improve the abnormal changes of blood routine and spleen and thymus tissue of rabbits. On the other hand, it can significantly increase the expression of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) in the spleen and thymus, and downregulate the expression of reactive oxygen species (ROS) and malondialdehyde (MDA). In addition, inflammatory factors interleukin-1 beta (IL-1β) and interleukin-6 (IL-6) and tumor necrosis factor alpha (TNF-α) in blood were also significantly inhibited; the expression of proliferating cell nuclear antigen (PCNA) in the spleen and thymus was also significantly increased after low-dose selenium treatment. Surprisingly, 0.4 mg/kg and 0.6 mg/kg of selenium did not effectively alleviate the immunotoxic effects caused by T-2 toxin, and cause damage to a certain extent. In summary, our results show that 0.2 mg/kg of SeMet can effectively alleviate the immunotoxicity caused by T-2 toxin. Selenium may protect rabbits from T-2 toxin by improving its antioxidant and anti-inflammatory capabilities.

Safety and efficacy of using heat-killed Lactobacillus plantarum L-137: High-dose and long-term use effects on immune-related safety and intestinal bacterial flora

Heat-killed Lactobacillus plantarum L-137 (HK L-137) promotes immune function in animals. In healthy people, T-cell proliferation was shown to be enhanced by taking 10 mg HK L-137 daily for 12 weeks. However, the safety and efficacy of higher doses or longer treatments have not yet been investigated in humans. To investigate the high-dose and long-term use effects of HK L-137 on immune-related safety and on host intestinal bacterial flora, 15 healthy volunteers took a daily HK L-137 (50 mg) preparation for 4 weeks. An additional 29 participants who regularly visited a clinic for health care took HK L-137 (10 mg) daily for 12 months. Measures for anthropometrics, hematology, biochemistry, and urinalysis were taken at scheduled timepoints for all participants. Stool and blood samples were also collected and evaluated for microbes and short-chain fatty acids (SCFA); isolated T-cells were assessed for levels of proliferation induced by phytohemagglutinin in the long-term study. Adverse events or shifts in clinical measures from normal ranges due to the dietary intervention were not observed in the high-dose or long-term studies. Long-term intake also did not result in immune exhaustion due to any chronic immunostimulation; ex vivo T-cell proliferation was significantly greater at 12 months than at baseline (p < 0.01). In addition, the Firmicutes/Bacteroidetes ratio in stool samples was significantly lower at 12 months than at baseline (p < 0.05) due to the long-term intake of the HK L-137. Lastly, fecal SCFA concentrations were significantly greater (p < 0.05) at 6 months than at baseline. From these data, it can be concluded that the efficacy of HK L-137 is maintained with no overt adverse effects as a result of high-dose and/or long-term consumption.

The Intersection of Regional Anesthesia and Cancer Progression: A Theoretical Framework.

The surgical stress and inflammatory response and volatile anesthetic agents have been shown to promote tumor metastasis in animal and in-vitro studies. Regional neuraxial anesthesia protects against these effects by decreasing the surgical stress and inflammatory response and associated changes in immune function in animals. However, evidence of a similar effect in humans remains equivocal due to the high variability and retrospective nature of clinical studies and difficulty in directly comparing regional versus general anesthesia in humans. We propose a theoretical framework to address the question of regional anesthesia as protective against metastasis.This theoretical construct views the immune system, circulating tumor cells, micrometastases, and inflammatory mediators as distinct populations in a highly connected system. In ecological theory, highly connected populations demonstrate more resilience to local perturbations but are prone to system-wide shifts compared with their poorly connected counterparts. Neuraxial anesthesia transforms the otherwise system-wide perturbations of the surgical stress and inflammatory response and volatile anesthesia into a comparatively local perturbation to which the system is more resilient. We propose this framework for experimental and mathematical models to help determine the impact of anesthetic choice on recurrence and metastasis and create therapeutic strategies to improve cancer outcomes after surgery.

Impact of Immune Function on Aerobic Capacity in Rats

BackgroundThe use of immunocompromised animals has been indispensable in cancer and immunology research. The use of exercise with these models in these areas of research has increased dramatically over the past decade, with little to no evidence of how immune modification of strains of animals is impacting exercise capacity and/or responses to training. This data is critical for extrapolation to exercise recommendations in humans as the majority of cancer patients do not present with primary immunodeficiency diseases. Recently, observations of lower skeletal muscle oxidative capacity between RNU (NIH Nude, immunocompromised) and Copenhagen rats (immunocompetent) have been speculated to result from possible differences between immune function in animals. The purpose of this study was to determine if there are differences in maximal aerobic capacity, through assessment of V̇o2max, between immune‐competent and ‐compromised animals of the same strain. We tested the hypothesis that immunocompromised animals would have a lower aerobic capacity than their immunocompetent counterparts.MethodsRNU 316 (NU−/−, T‐cell deficient; n=10) and RNU 118 (NU+/−, no deficiency; n=10) male rats (~4 months old) were obtained from Charles River. Both groups were acclimated to motorized treadmill running at varying speeds (20–40 m/min) for 3–5 sessions before beginning V̇o2max assessment. V̇o2max graded treadmill testing consisted of a 2‐minute warm‐up at 25 m/min at a 5% incline followed by 2 minutes at 35 m/min, and subsequent increases in speed of 5 m/min every minute after until a plateau was seen in oxygen consumption with increased speed, or test termination was required for safety of the animal. Tests were repeated a minimum of two times (3 maximally) per animal to verify maximum values were achieved.ResultsThere were no significant differences in body mass (NU−, 347±12 g, NU+, 321±8 g; p=0.17). V̇o2max was lower in NU− vs NU+ animals (80.0±1.5 vs. 88.4±1.9 ml·min−1·kg−1; p&lt;0.01). There was a significant difference in respiratory exchange ratio (RER) at V̇o2max (NU−, 1.05±0.01 vs. NU+, 0.98±0.01; p&lt;0.05), but no difference in CO2 production (NU−, 83.7±1.3 vs. NU+, 86.7±1.5 ml·min−1·kg−1; p&gt;0.05).ConclusionThis study suggests that immunocompromised animals have a decreased aerobic capacity compared to immunocompetent counterparts. This should be considered when both designing exercise interventions in immunedeficient/compromised animals as well as interpretations derived from such studies.Support or Funding InformationNIH HL137156‐01A1ACS RSG‐14‐150‐01‐CCE

Measuring Peripheral and Some Mucosal Immune Cells to Better Understand Immunomodulation by T-2 Toxin in Broilers

SUMMARY The ability of naturally-occurring low levels of food-borne toxins and environmental contaminants to alter immune function in animals has been the subject of increasing interest in recent years. A total of 96 1-d-old broiler chickens were used to evaluate the effect of feeding naturally contaminated rations with low levels (800 ppb) of T-2 toxin and the protective effect of a commercial mycotoxin eliminator on circulating and intestinal immune cells, blood biochemistry, and hematological variables. Although there were no effects on body weight, contamination with T-2 toxin lead to a significant increase in serum levels of total protein (mainly due to increased albumin levels), alkaline phosphatase (AP), and aspartate aminotransferase (AST), and increased jejunal levels of CD3+ and Goblet cells at day 28. T-2 toxin significantly altered immune parameters, as was demonstrated by alterations in the number of circulating macrophages and suppressor macrophages, B-lymphocytes, T-lymphocytes from mucosa, virgin cytotoxic T-lymphocytes, and terminally activated cytotoxic T-lymphocytes compared to uncontaminated control birds. The use of a mycotoxin eliminator partly compensated for the changes in immune cells. The importance of low levels of mycotoxins is still debatable. However, in this research, it is clearly demonstrated that T-2 toxins affects immune responses, possibly due to impaired gut barrier functioning and metabolic changes.

Dehydroepiandrosterone alleviates E. Coli O157:H7-induced inflammation by preventing the activation of p38 MAPK and NF-κB pathways in mice peritoneal macrophages

As an important metabolite in cholesterol metabolism, dehydroepiandrosterone (DHEA) can modulate the immune function in animals and humans, but the underlying mechanism is still unclear. The present study investigated the effect and mechanism of DHEA’s anti-inflammatory action in primary mice peritoneal macrophages infected with E. coli O157:H7. The finding showed that DHEA improved the phagocytic ability in E. coli O157:H7-infected macrophages. DHEA inhibited the cytokines (including tumor necrosis factor-α, interleukin-1β and interleukin-6) secretion in E. coli O157:H7-infected macrophages. The inducible nitric oxide synthase and cyclooxygenase-2 protein level were significantly decreased in E. coli O157:H7-infected macrophages treated with DHEA. In addition, DHEA markedly decreased the phospho (p)-p38 MAPK protein level in E. coli O157:H7-infected macrophages. Furthermore, DHEA prevented the nuclear translocation of NF-κB by decreasing of p-IκB-α protein level in E. coli O157:H7-infected macrophage; and these effects of DHEA were heightened when the cells were pre-treated with p38 MAPK inhibitor SB203580. Our data indicated that DHEA alleviates the pro-inflammatory mediator production in E. coli O157:H7-infected mice peritoneal macrophages; and this beneficial action associated with it prevents the activation of p38 MAPK and NF-κB signaling pathway.

Dehydration enhances innate immunity in a semiaquatic snake from the wet-dry tropics.

Dehydration is considered a physiological challenge, and many organisms live in environments that undergo periods of reduced water availability that can lead to dehydration. Recent studies have found a positive relationship between dehydration and innate immune function in animals adapted to xeric or semixeric environments. To explore the generality of this relationship, we examined the impact of dehydration on innate immune performance in water pythons (Liasis fuscus), a semiaquatic snake from the wet-dry tropics of Australia. We collected blood samples from male and female water pythons held in the laboratory without food and water for 4 weeks. We also collected blood from free-ranging snakes throughout the Austral dry-season. We evaluated plasma osmolality and innate immune function (agglutination, lysis, and bacterial-killing ability) and found that increased osmolality, whether manipulated in the laboratory or as a result of natural water limitation, resulted in enhanced aspects of innate immune performance. Counter-intuitively, snakes in the wild became more hydrated as the dry season progressed, suggesting the dehydrated snakes move to water sources periodically to rehydrate. Comparing our data with those from previous studies, we suspect species divergence in the level of dehydration (i.e., hyperosmolality) that triggers enhanced immune capabilities.

High Body Mass Has No Effect on Cellular and Innate Immunity in Siberian Hamsters (&amp;lt;i&amp;gt;Phodopus sungorus&amp;lt;/i&amp;gt;)

Body mass is considered to be related with immune function in animals. Our aim was to test the hypothesis that cellular and innate immunity would be suppressed in high body mass of Siberian hamsters (Phodopus sungorus). Six heavier (high body mass, HBM) and six lighter (low body mass, LBM) hamsters were selected from 28 male hamsters. Body mass, body fat mass, wet spleen mass and blood glucose levels were significantly higher in the HBM group than in the LBM group. However, phytohaemagglutinin response, serum bacteria killing capacity and white blood cells did not differ between the two groups, suggesting cellular and innate immunity was not impaired in high body mass of hamsters. There was no correlation between cellular, innate immunity and body mass, body fat mass and glucose levels, suggesting cellular and innate immunity was not suppressed by higher body mass, body fat mass and glucose levels. In summary, cellular and innate immunity was not impaired in the HBM hamsters compared with the LBM hamsters.

Effects of Methionine on the Immune Function in Animals

Nutrition and immunity are the hot topics in animal’s production, and the effects of methionine on the immunity are already confirmed as the deep research on the nutrition of amino acid and immune function. However, the relationship of the methionine and immunity has not been elucidated clearly, this review aims to clarify the effects of methionine on immune function in the aspects of growth and development immune organs, the histological structure of the immune organs, non-specific immunity, humoral immunity, cellular immunity and cytokines, and to provide foundations for further studies on the relationship between methionine and immune function.

Tributyltin exposure alters cytokine levels in mouse serum

Tributyltin (TBT), a toxic environmental contaminant, has been widely utilized for various industrial, agricultural and household purposes. Its usage has led to a global contamination and its bioaccumulation in aquatic organisms and terrestrial mammals. Previous studies suggest that TBT has debilitating effects on the overall immune function of animals, rendering them more vulnerable to diseases. TBT (at concentrations that have been detected in human blood) alters secretion of inflammatory cytokines from human lymphocytes ex vivo. Thus, it is important to determine if specified levels of TBT can alter levels of cytokines in an in vivo system. Mice were exposed to biologically relevant concentrations of TBT (200, 100 or 25 nM final concentrations). The quantitative determination of interferon (IFN)-γ, tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL2, IL5, IL7, IL12βp40, IL13, IL15, keratinocyte chemoattractant (KC), macrophage inflammatory protein 1β (MIP), MIP2 and regulated on activation normal T-cell-expressed and secreted (RANTES) was performed in mouse sera by MAGPIX analysis and Western blot. Results indicated alterations (both decreases and increases) in several cytokines. The pro-inflammatory cytokines IFNγ, TNFα, IL-1β, IL-2, IL5, IL12βp40 and IL-15 were altered as were the chemokines MIP-1 and RANTES and the anti-inflammatory cytokine IL-13. Increases in IFNγ and TNFα were seen in the serum of mice exposed to TBT for less than 24 h. Levels of IL1β, IL-12 βp40, IL-5 and IL-15 were also modulated in mouse serum, depending on the specific experiment and exposure level. IL-2 was consistently decreased in mouse serum when animals were exposed to TBT. There were also TBT-induced increases in MIP-1β, RANTES and IL-13. These results from human and murine samples clearly suggest that TBT exposures modulate the secretion inflammatory cytokines.

Effect of Fluoride on Endocytosis and Surface Marker Expression Levels of Mouse B Cells In Vitro

Background/Aims: To clarify the effect of fluoride on splenic B cells, the endocytosis and surface marker expression levels of mouse splenic B cells were detected in vitro by flow cytometry. Methods: Cells were stimulated with 10 µg/mL lipopolysaccharide (LPS) and varying concentrations of Sodium Fluoride (NaF) (0, 50 µM, 100 µM, 500 µM, 1000 µM). Results: The results demonstrated that the endocytic capacity of B cells was enhanced by NaF at 50µM. NaF significantly enhanced CD80 expression at 50 µM and decreased CD86 expression at 500 µM. CD40 and CD138 expression on B cells were down-regulated at varying high concentrations of NaF. Conclusion: our results showed that the endocytic capacity, expression levels of CD40 and CD80 of B cells changed significantly at lower concentrations, whereas expression levels of CD138 and CD86 changed significantly at higher concentrations, suggesting that fluoride could inhibit immune function in animals.

The characterization of a concentration-sensitive α-adrenergic-like octopamine receptor found on insect immune cells and its possible role in mediating stress hormone effects on immune function

Octopamine (OA), the insect equivalent of norepinephrine, links the nervous system and immune system in insects. This study examines the underlying molecular mechanisms (i.e. second messenger systems) mediating OA effects on insect immune cells. At low concentrations (<1μM), OA stimulatedhemocyte spreading and phagocytosis in the larval Lepidopteran (caterpillar) Chilo suppressalis, whereas at high concentrations (>10 μM), OA inhibited hemocyte spreading and phagocytosis. Similarly, OA concentration had differential effects on two intracellular signaling pathways, Ca(2+) and cAMP. Low concentrations of OA increased intracellular Ca(2+), but only high concentrations of OA (>1 μM) led to an increase in both Ca(2+) and cAMP. We identified an α-adrenergic-like octopamine receptor in this species (CsOA1) and confirmed that it is expressed in hemocytes. After heterologous expression in HEK-293 cells, the CsOA1 receptor produced the same OA concentration-dependent responses on intracellular Ca(2+) and cAMP as had been observed in hemocytes. These findings support earlier work showing that OA has both stimulatory and suppressive effects on immune responses, depending on the OA concentration. Our evidence suggests that these biphasic effects are mediated by an octopamine receptor signaling through intracellular Ca(2+) and cAMP second messenger pathways. Stress hormones/neuromodulators have complex effects on immune function in animals across phyla. This complexity may be mediated, in part, by conserved connections between adrenergic-like G-coupled protein receptors and second messenger systems.