To the microwave irradiation safety hazards caused by the co-construction of towers in smart grids, this paper investigates the effects of microwave irradiation in the power environment on the biological motion balance perception function. Firstly, simulation of microwave signals in the electric power environment, i.e., low-frequency harmonics and high-frequency carriers, were realized by signal modulation and applied in four types of behavior testing scenarios. Then, determining rats as target organisms to replace workers and randomly dividing into groups in proportion: open field, rotating rod fatigue, beam walking and forced swimming. Configuring radar with various parameters to match the electric power irradiation scene and stimulate rats, monitoring the abnormal behavior by image processing module, including posture, motion trajectory, distance, and other parameters. The experimental result showed that exposed to microwaves induce rats motor ability decline, balance perception imbalance, together with paralysis within long-term exposure, and its locomotor activity, coordination, posture control and reaction time all exhibit varying degrees of weakening. These findings indicate that microwave irradiation in electric power environment may pose significant health and safety risks for worker.