Illumination Mode Research Articles

On the use of isotopic differences of carbon fractions of biomass in plants to study transport flows and source-sink relations under different light conditions

It is shown that the differences in the isotopic composition of carbon in the water-soluble and water-insoluble fractions of plant leaf biomass, as well as phloem, are evolutionarily determined. They associated with metabolic reactions during assimilation and photorespiration and do not depend on the illumination mode and on the spectral ranges of headlights used in illumination. The above isotopic shifts are the cause of isotopic differences in assimilatory and photorespiratory carbon stocks that feed various metabolic processes. Due to the strict temporal and spatial organization of metabolism, carbon fluxes from the funds retain isotopic differences without complete mixing. The differences in the isotopic composition of carbon of the water-soluble fraction of biomass and carbon of phloem juice from carbon of the water-insoluble fraction are small (1–3%), but they are quite stable and easily fixed. The carbon of the water-soluble fraction is very close in isotopic composition to the carbon of the phloem and is noticeably enriched with the isotope 13C relative to the water-insoluble fraction, which makes it possible to use it as a marker in the study of assimilate transport in plants, especially during budding and fruiting. It is shown that the reason for the enrichment of autotrophic organs and tissues with isotope 12C relative to carbon of heterotrophic parts of the plant is the predominant participation in their formation of an isotopically light assimilation fund, whereas an isotopically heavy photorespiratory fund takes part in the formation of heterotrophic organs. It is shown that the manifestation of the formation of two isotopically different funds is the discovered relationship of the carbon isotope composition of leaves with their age.

Quasi-visualizable detection of deep sub-wavelength defects in patterned wafers by breaking the optical form birefringence

Abstract In integrated circuit (IC) manufacturing, fast, nondestructive, and precise detection of defects in patterned wafers, realized by bright-field microscopy, is one of the critical factors for ensuring the final performance and yields of chips. With the critical dimensions of IC nanostructures continuing to shrink, directly imaging or classifying deep-subwavelength defects by bright-field microscopy is challenging due to the well-known diffraction barrier, the weak scattering effect, and the faint correlation between the scattering cross-section and the defect morphology. Herein, we propose an optical far-field inspection method based on the form-birefringence scattering imaging of the defective nanostructure, which can identify and classify various defects without requiring optical super-resolution. The technique is built upon the principle of breaking the optical form birefringence of the original periodic nanostructures by the defect perturbation under the anisotropic illumination modes, such as the orthogonally polarized plane waves, then combined with the high-order difference of far-field images. We validated the feasibility and effectiveness of the proposed method in detecting deep subwavelength defects through rigid vector imaging modeling and optical detection experiments of various defective nanostructures based on polarization microscopy. On this basis, an intelligent classification algorithm for typical patterned defects based on a dual-channel AlexNet neural network has been proposed, stabilizing the classification accuracy of λ/16-sized defects with highly similar features at more than 90%. The strong classification capability of the two-channel network on typical patterned defects can be attributed to the high-order difference image and its transverse gradient being used as the network's input, which highlights the polarization modulation difference between different patterned defects more significantly than conventional bright-field microscopy results. This work will provide a new but easy-to-operate method for detecting and classifying deep-subwavelength defects in patterned wafers or photomasks, which thus endows current online inspection equipment with more missions in advanced IC manufacturing.

Active illumination mode with checkerboard pattern in focus variation microscopy: Analysis and application

Optical measurement methods for surface topography offer the advantages of high accuracy, rapid measurement, and non-destructiveness. Each method has its own suitable application scenarios. Among them, focus variation microscopy is extensively employed in precision manufacturing, aerospace, and medical industries due to its ability to measure rough and large slopes surfaces. However, since the measurement depends on local grayscale differences between focused and blurred images, it cannot measure surfaces with low reflectivity or insufficient texture information. In this work, we propose an active illumination mode for focus variation method that utilizes a digital micromirror device (DMD) to generate a checkerboard pattern. This method introduces additional texture information, resulting in a usable local gradient of image grayscale. Additionally, we analyze the selection criteria for the checkerboard pattern parameters, including the period and light-dark ratio. Furthermore, measurements of two standard steps with different heights demonstrate that the measurement repeatability of the proposed method can reach the nanometer level, rendering it suitable for high-precision measurements. More importantly, the measurement noise results indicate significantly superior performance of active illumination mode compared to the uniform illumination mode. Finally, we reconstruct the surface topography of the microchannels in a microfluidic chip through the encapsulation layer, demonstrating the feasibility of the proposed method.

An Accurate Non-contact Photoplethysmography via Active Cancellation of Reflective Interference.

Imaging Photoplethysmography (IPPG) is an emerging and efficient optical method for non-contact measurement of pulse waves using an image sensor. While the contactless way brings convenience, the inevitable distance between the sensor and the subject results in massive specular reflection interference on the skin surface, which leads to a low Signal to Interference plus Noise Ratio (SINR) of IPPG. To ease this challenge, this work proposes a novel modulation illumination approach to measure the accurate arterial pulse wave via surface reflection interference isolation from IPPG. Based on the proposed skin reflection model, a specific modulation illumination is designed to separate the surface reflections and obtain the subcutaneous diffuse reflections containing the pulse wave information. Compared with the results under ambient illumination and constant supplemental illumination, the SINR of the proposed method is improved by 4.56 and 3.74 dB, respectively.

Full-angle single-shot quantitative phase imaging based on Kramers-Kronig relations.

As a non-interference and non-iterative method, annular-illumination quantitative phase imaging based on Kramers-Kronig relations (AIKK) can realize phase measurement with full-angle resolution enhancement under multiple exposures. In order to completely record the object spectrum with a single shot, we proposed a colorful complementary illumination method in the recording process. The angle of this illumination mode is not symmetrical with each other, so the spectrum between the three channels can complement each other to avoid spectrum loss caused by spectrum conjugation. Meanwhile, the three spectral segments of full-angle information spectrum respectively carried by three wavelengths can be recorded. Additionally, the numerical filter is applied to correct the overlapped spectrum in the reconstruction process. Simulation and experimental results show that this method can achieve high spatiotemporal resolution quantitative phase measurement.

Heat-Annealed Zinc Oxide on Flexible Carbon Nanotube Paper and Exposed to Gradient Light to Enhance Its Photoelectric Response.

Buckypaper (BP), a flexible and porous material, exhibits photovoltaic properties when exposed to light. In this study, we employed radio frequency (RF) sputtering of zinc oxide (ZnO) followed by rapid thermal annealing to enhance the photovoltaic response of BP. We investigated the impact of various sputtering parameters, such as the gas flow ratio of argon to oxygen and deposition time, on the morphology, composition, resistivity, and photovoltaic characteristics of ZnO-modified BP. Additionally, the photovoltaic performance of the samples under different illumination modes and wavelengths was compared. It was found that optimal sputtering conditions-argon to oxygen flow ratio of 1:2, deposition time of 20 min, and power of 100 watts-resulted in a ZnO film thickness of approximately 45 nanometers. After annealing at 400 °C for 10 min, the ZnO-modified BP demonstrated a significant increase in photocurrent and photovoltage, along with a reduction in resistivity, compared to unmodified BP. Moreover, under gradient illumination, the ZnO-modified BP exhibited a photovoltage enhancement of 14.70-fold and a photocurrent increase of 13.86-fold, compared to uniform illumination. Under blue light, it showed a higher photovoltaic response than under other colors. The enhancement in photovoltaic response is attributed to the formation of a Schottky junction between ZnO and BP, an increased carrier concentration gradient, and an expanded light absorption spectrum. Our results validate that ZnO sputtering followed by annealing is an effective method for modifying BP for photovoltaic applications such as solar cells and photodetectors.

The investigation of thickness-dependent mono-fractal, optical and optoelectronics properties of sputtered silver thin film for silicon solar cell

Here we report, the room temperature deposition of silver (Ag) thin films on silicon, glass and silicon solar cell substrates by magnetron sputtering. The effect of thickness on the deposited thin films and their morphological, fractal, optical and solar cell parameters have been extensively investigated. AFM results show that the Ag thin films are composed of different-size of nano-grains and the grain size increases with increasing thickness. Mono-fractal parameters, namely fractal dimension (Df) and Hurst exponent (H), were determined from the power spectral density function. X-ray photoelectron spectroscopy studies indicate that the deposited samples exhibit the presence of oxidation state of Ag (0) in thin films, confirming the stable structure. The optical analysis shows that the transmittance of the deposited thin films decreases with increasing thickness, on the other hand, the silver thin film with thickness of 20 nm shows surface Plasmon resonance (SPR) at 436 nm. Current-voltage (I–V) curves were measured in dark and under illumination mode at standard testing conditions, i.e., 300K, 1000 W/m2 and 1.5 AM global using a solar simulator. These measurement studies indicate that thicknesses are effectively impacts on photovoltaic performance. The calculated values of change in solar efficiency (Δη) are 0.26, −0.20, −0.76 and −6.88 % for thin films with nominal thickness 0.5, 1, 2 and 20 nm respectively.

Modulated illumination microscopy: Application perspectives in nuclear nanostructure analysis.

The structure of the cell nucleus of higher organisms has become a major topic of advanced light microscopy. So far, a variety of methods have been applied, including confocal laser scanning fluorescence microscopy, 4Pi, STED and localisation microscopy approaches, as well as different types of patterned illumination microscopy, modulated either laterally (in the object plane) or axially (along the optical axis). Based on our experience, we discuss here some application perspectives of Modulated Illumination Microscopy (MIM) and its combination with single-molecule localisation microscopy (SMLM). For example, spatially modulated illumination microscopy/SMI (illumination modulation along the optical axis) has been used to determine the axial extension (size) of small, optically isolated fluorescent objects between ≤ 200nm and ≥ 40nm diameter with a precision down to the few nm range; it also allows the axial positioning of such structures down to the 1nm scale; combined with laterally structured illumination/SIM, a 3D localisation precision of ≤1nm is expected using fluorescence yields typical for SMLM applications. Together with the nanosizing capability of SMI, this can be used to analyse macromolecular nuclear complexes with a resolution approaching that of cryoelectron microscopy.

Single-shot synthetic wavelength imaging: Sub-mm precision ToF sensing with conventional CMOS sensors

We present a novel single-shot interferometric ToF camera targeted for precise 3D measurements of dynamic objects. The camera concept is based on Synthetic Wavelength Interferometry, a technique that allows retrieval of depth maps of objects with optically rough surfaces at submillimeter depth precision. In contrast to conventional ToF cameras, our device uses only off-the-shelf CCD/CMOS detectors and works at their native chip resolution (as of today, theoretically up to 20 Mp and beyond). Moreover, we can obtain a full 3D model of the object in single-shot, meaning that no temporal sequence of exposures or temporal illumination modulation (such as amplitude or frequency modulation) is necessary, which makes our camera robust against object motion.In this paper, we introduce the novel camera concept and show the first measurements that demonstrate the capabilities of our system. We present 3D measurements of small (cm-sized) objects with > 2 Mp point cloud resolution (the native pixel resolution of our used detector) and up to sub-mm depth precision. We also report a “single-shot 3D video” acquisition and a first single-shot “Non-Line-of-Sight” measurement. Our technique has great potential for high-precision applications with dynamic object movement, e.g., in AR/VR, industrial inspection, medical imaging, and imaging through scattering media like fog or human tissue.

Conjugated Polythiophene Frameworks as a Hole‐Selective Layer on Ta3N5 Photoanode for High‐Performance Solar Water Oxidation

AbstractDiscovering a competent charge transport layer promoting charge separation in photoelectrodes is a perpetual pursuit in photoelectrochemical (PEC) water splitting to achieve high solar‐to‐hydrogen (STH) conversion efficiency. Here, a conjugated polythiophene framework (CPF‐TTB) on Ta3N5 is elaborately electropolymerized, substantiating the hole transport behavior in their heterojunction. Tailored band structures of the CPF‐TTB/Ta3N5 reinforce the separation of photogenerated carriers, elevating a fill factor of the photoanode modified with a cocatalyst. The enhanced hole extraction enables the NiFeOx/CPF‐TTB/Ta3N5/TiN photoanode to generate a remarkable water oxidation photocurrent density of 9.12 mA cm−2 at 1.23 V versus the reversible hydrogen electrode. A tandem device combining the photoanode with a perovskite/Si solar cell implements an unbiased solar water splitting with a STH conversion efficiency of 6.26% under parallel illumination mode. This study provides novel strategies in interface engineering for metal nitride‐based photoelectrodes, suggesting a promise of the organic–inorganic hybrid photoelectrode for high‐efficiency PEC water splitting.

StayGold photostability under different illumination modes

StayGold is a bright fluorescent protein (FP) that is over one order of magnitude more photostable than any of the currently available FPs across the full range of illumination intensities used in widefield microscopy and structured illumination microscopy, the latter of which is a widefield illumination-based technique. To compare the photostability of StayGold under other illumination modes with that of three other green-emitting FPs, namely EGFP, mClover3, and mNeonGreen, we expressed all four FPs as fusions to histone 2B in HeLa cells. Unlike the case of widefield microscopy, the photobleaching behavior of these FPs in laser scanning confocal microscopy (LSCM) is complicated. The outstanding photostability of StayGold observed in multi-beam LSCM was variably attenuated in single-beam LSCM, which produces intermittent and instantaneously strong illumination. We systematically examined the effects of different single-beam LSCM beam-scanning patterns on the photostability of the FPs in living HeLa cells. This study offers relevant guidelines for researchers who aim to achieve sustainable live cell imaging by resolving problems related to FP photostability. We also provide evidence for measurable sensitivity of the photostability of StayGold to chemical fixation.

Dual-wavelength resolution matching digital holographic microscopy using one path structured illumination

A dual-wavelength resolution-matching digital holographic microscopy with one path structured illumination is presented, which can improve phase imaging resolution. In this configuration, a dual-wavelength hologram with two illumination modes corresponding to two different wavelengths can be flexibly recorded at one shot, and the imaging resolutions under the two wavelengths can reach equivalence by setting the modulation frequency of structure illumination. The expression for calculating the modulation frequency of structured illumination in a two-wavelength system is given. By setting the proper modulation frequency, the resolution of dual-wavelength phase imaging can be enhanced, which is verified in the phase imaging experiment of a USAF quantitative phase target. The dual-wavelength reconstructed phase map of paramecia cells exhibit the internal macronucleus, food vacuole and cilia. The imaging results demonstrate the resolution-matching approach using one path structured-illumination in effect for the improvement of phase imaging resolution.

Online Denoising Single-Pixel Imaging Using Filtered Patterns

Noise is inevitable in single-pixel imaging (SPI). Although post-processing algorithms can significantly improve image quality, they introduce additional processing time. To address this issue, we propose an online denoising single-pixel imaging scheme at the sampling stage, which uses the filter to optimize the illumination modulation patterns. The image is retrieved through the second-order correlation between the modulation patterns and the intensities detected by the single-pixel detector. Through simulations and experiments, we analyzed the impact of sampling rate, noise intensity, and filter template on the reconstructed images of both binary and grayscale objects. The results demonstrate that the denoising effect is comparable to the imaging-first followed by post-filtering procedures, but the post-processing time is reduced for the same image quality. This method offers a new way for rapid denoising in SPI, and it should be particularly advantageous in applications where time-saving is of paramount importance, such as in image-free large target classification.

Imperceptible Physical Attack against Face Recognition Systems via LED Illumination Modulation

Imperceptible Physical Attack against Face Recognition Systems via LED Illumination Modulation

Towards a Better Understanding of the Back-Side Illumination Mode on Photocatalytic Metal–Organic Chemical Vapour Deposition Coatings Used for Treating Wastewater Polluted by Pesticides

Pesticides are emerging contaminants that pose various risks to human health and aquatic ecosystems. In this work, diuron was considered as a contaminant model to investigate the influence of the back-side illumination mode (BSI) on the photocatalytic activity of TiO2 coatings grown on Pyrex plates by metal–organic chemical vapour deposition (MOCVD). A photoreactor working in recirculation mode was irradiated at 365 nm with ultraviolet A (UVA) light-emitting diodes in BSI. The degradation of diuron and its transformation products was analysed by high-performance liquid chromatography, ion chromatography, and total organic carbon analysis. The coatings were characterised by X-ray diffraction analysis and scanning electron microscopy. Five coatings containing 3, 7, 10, 12 and 27 mg of TiO2 exhibited different morphology, crystallinity, thickness and photocatalytic activities. The morphology and crystallinity of the coatings had no significant influence on their photocatalytic activity, unlike their mass and thickness. TiO2 contents less than 10 mg limit the photocatalytic activity, whereas those greater than 15 mg are inefficient in the BSI because of their thickness. The maximum efficiency was achieved for coatings of thickness 1.8 and 2 µm with TiO2 contents of 10 and 12 mg, revealing that the photocatalyst thickness controls the photocatalytic efficiency in the BSI.

High-performance self-driven ultraviolet-visible photodetector based on type-II WS2/ReSe2 van der Waals heterostructure

Van der Waals (vdWs) heterojunctions constructed by varied two-dimensional materials provide a broader platform over traditional semiconductors to fabricate high-performance optoelectronic devices due to their superior optoelectronic properties. This paper demonstrates a photodetector based on WS2/ReSe2 vdWs heterostructure with photovoltaic effect. The ultraviolet photoelectron spectroscopy (UPS) confirms a type-II band alignment of the p-n junction with a conduction band offset of approximately 0.1 eV and a valence band offset of 0.78 eV. The photoresponse characteristics indicate that the device operates in photovoltaic mode under 350 nm illumination and photoconductive mode at 638 nm, respectively. Under 350 nm illumination, this photovoltaic device exhibits the extraordinary photoresponsivity of 2.78 mA/W and the specific detectivity of 1.05 × 1010 Jones in self-powered mode, and the corresponding values are 1.35 A/W and 5.88 × 1011 Jones under external bias, indicating synergistic development of the photoresponsivity and detection. In contrast, the photodetector shows ultrafast rise/decay times of 4/5 µs at 350 nm and a fast response speed of 50/55 µs at 638 nm, which is significantly rapider than most self-driven photodetectors currently published. Therefore, this work provides an ideal platform for the formation of high-performance photovoltaic device based on WS2/ReSe2 vdWs heterojunctions.

Doppler Time-of-Flight Rendering

We introduce Doppler time-of-flight (D-ToF) rendering, an extension of ToF rendering for dynamic scenes, with applications in simulating D-ToF cameras. D-ToF cameras use high-frequency modulation of illumination and exposure, and measure the Doppler frequency shift to compute the radial velocity of dynamic objects. The time-varying scene geometry and high-frequency modulation functions used in such cameras make it challenging to accurately and efficiently simulate their measurements with existing ToF rendering algorithms. We overcome these challenges in a twofold manner: To achieve accuracy, we derive path integral expressions for D-ToF measurements under global illumination and form unbiased Monte Carlo estimates of these integrals. To achieve efficiency, we develop a tailored time-path sampling technique that combines antithetic time sampling with correlated path sampling. We show experimentally that our sampling technique achieves up to two orders of magnitude lower variance compared to naive time-path sampling. We provide an open-source simulator that serves as a digital twin for D-ToF imaging systems, allowing imaging researchers, for the first time, to investigate the impact of modulation functions, material properties, and global illumination on D-ToF imaging performance.

A non-iterative frame-reduced structured illumination microscopy using checkerboard modulation

Structured illumination microscopy (SIM) is a tremendously potential super-resolution microscopy technique requiring nine raw images to enhance two-fold lateral resolution. For SIM, it is a challenging dilemma to achieve super-resolution imaging with a reduced number of images and fast data processing speed at the same time. Here, we demonstrate a new SIM reconstruction framework that uses only four raw images without requiring extensive iterative computation. The four-frame method is achieved by applying checkerboard pattern illumination modulation to supersede the sinusoidal fringe illumination. It is worth noting that the proposed scheme prominently shortens image acquisition time, combined with a remarkably higher image reconstruction rate compared to current frame-reduced methods. Meanwhile, the reconstruction process is non-iterative and unlimited by the field of view size. Consequently, our work is expected to bring about breakthroughs in facilitating the improvement of real-time live cell observation.

Non-iterative reconstruction of interferenceless coded aperture correlation holography enabled high quality three-dimensional imaging

Interferenceless coded aperture correlation holography (I-COACH) is a rapidly developing indirect three-dimensional (3D) imaging technique. However the ideal point spread function of imaging system is not obtained in most cases, the object hologram is usually correlated with the system response of a limited-size pinhole during the reconstruction process. As a result, the quality of reconstructed images is contaminated by noise. In this paper, the system response of a limited-size pinhole is regarded as the convolution of the pinhole (as an object) intensity and the ideal point spread function of I-COACH, a mathematical analysis of the background noise is conducted and a general high-quality reconstruction mathematic model of I-COACH system is derived when a limited-size pinhole is used to record point spread hologram, in which the reconstructions and noise is separated. Then a non-iterative cross-correlation reconstruction algorithm has been developed here to achieve high-quality 3D reconstruction. The proposed method has been implemented on a regular I-COACH system in both transmission as well as reflection illumination modes to demonstrate the imaging ability of our method. The experimental results show that high quality reconstructions can be achieved by our proposal with a single-exposure object hologram.

Super-resolution reconstruction of structured illumination microscopy based on pixel reassignment

In this work, we report a pixel reassignment based super-resolution reconstruction algorithm for structured illumination microscopy (entitled PR-SIM). PR-SIM provides a twofold theoretical resolution enhancement by reassigning the pixels in raw SIM images with respect to the center of each illumination fringe and applying further deconvolution. By comparing with frequency domain based algorithms, PR-SIM is more immune to fringe distortion and, hence, it is more suited for large-field SIM in that it processes the raw images locally. Meanwhile, the reconstruction speed of PR-SIM can be enhanced by skipping empty regions in the image and further enhanced by employing GPU-base parallel calculation. Overall, we can envisage that the PR-SIM can be extended for other illumination modulation based microscopic techniques.