Identity Of Pathogenic Bacteria Research Articles

Study on antibacterial activity of medicinal herbs against urinary tract infection (UTI) pathogens

This study is aimed in the direction of the dedication of antimicrobials on native herbals in Tirunelveli district. Tamil Nadu. India. In the direction of fine pathogenic microorganisms isolated from the urinary tract of humans. The urine pattern was gathered from patients who have been processed for the isolation & identity of pathogenic bacteria. The extracts have been subjected for screening of in vitro antibacterial activity against decided primary urinary tract contamination (UTI) causing pathogens viz. (Staphylococcus aureus, Klebsiella pneumonia, Salmonalla typhi, Escherichia coli, and Streptococcus pyogenes) at the concentrations of 25 µl to 200 µl via disc diffusion assay technique. The effects of antibacterial activity discovered that the all extract confirmed suitable inhibitory interest against all the tested pathogens. The leaves of herbs, specifically Azadirachta indica, Ocimum sanctum, and Phyllanthus niruri were taken and an ethanol extract changed into prepared.

Does Early Debridement, Antibiotic Therapy and Implant Retention (DAIR) have a Role in Managing Periprosthetic Joint Infection of the Knee in Indian Scenario: A Retrospective Analysis of Outcomes.

To report outcomes of Debridement, Antibiotic therapy and Implant Retention (DAIR) for periprosthetic knee joint infections (PJI) in the Indian population and to study factors influencing outcomes. This was a Retrospective study of 80 cases of acute PJI after total knee arthroplasty who were treated by DAIR, within 2weeks of onset of infection. A standardised institutional management protocol was applied to all cases. Patients were followed up for a minimum 1year. Outcomes of DAIR were classified as successful or unsuccessful based on resolution or persistence of infection, and subsequent requirement of revision surgery. Influence of factors, like comorbidities, culture status and microbiological characteristics of causative organism, on outcomes was assessed. Overall 55 patients (68.75%) had successful eradication of infection after DAIR. 27 (33.7%) patients were culture negative and 53 (66.2%) patients grew organisms on culture. There was no statistically significant difference in outcomes (p = 0.082) between culture-positive cases (69.8% success rate) and (66.7% success rate) in culture negative cases. Furthermore, no difference in outcomes was observed in culture-positive patients between those who grew Gram-positive organisms versus Gram-negative organisms (p = 0.398) Similarly, patient comorbidities did not significantly alter the outcomes after DAIR (p = 0.732). Our study demonstrates that early DAIR within 2weeks of onset of infection using a standard protocol during surgery and postoperatively can result in good outcomes. Patient comorbidities, culture status (positive versus negative), Gram staining characteristics of organisms and the identity of pathogenic bacteria did not influence outcomes of DAIR for acute PJI.

Determining the prevalence, identity and possible origin of bacterial pathogens in soil.

Soil biomes are vast, exceptionally diverse and crucial to the health of ecosystems and societies. Soils also contain an appreciable, but understudied, diversity of opportunistic human pathogens. With climate change and other forms of environmental degradation potentially increasing exposure risks to soilborne pathogens, it is necessary to gain a better understanding of their ecological drivers. Here we use the Galleria mellonella insect virulence model to selectively isolate pathogenic bacteria from soils in Cornwall (UK). We find a high prevalence of pathogenic soil bacteria with two genera, Providencia and Serratia, being especially common. Providencia alcalifaciens, P. rustigianii, Serratia liquefaciens and S. plymuthica strains were studied in more detail using phenotypic virulence and antibiotic resistance assays and whole-genome sequencing. Both genera displayed low levels of antibiotic resistance and antibiotic resistance gene carriage. However, Serratia isolates were found to carry the recently characterized metallo-β-lactamase blaSPR-1 that, although not conferring high levels of resistance in these strains, poses a potential risk of horizontal transfer to other pathogens where it could be fully functional. The Galleria assay can be a useful approach to uncover the distribution and identity of pathogenic bacteria in the environment, as well as uncover resistance genes with an environmental origin.

Using the wax moth larva Galleria mellonella infection model to detect emerging bacterial pathogens.

Climate change, changing farming practices, social and demographic changes and rising levels of antibiotic resistance are likely to lead to future increases in opportunistic bacterial infections that are more difficult to treat. Uncovering the prevalence and identity of pathogenic bacteria in the environment is key to assessing transmission risks. We describe the first use of the Wax moth larva Galleria mellonella, a well-established model for the mammalian innate immune system, to selectively enrich and characterize pathogens from coastal environments in the South West of the UK. Whole-genome sequencing of highly virulent isolates revealed amongst others a Proteus mirabilis strain carrying the Salmonella SGI1 genomic island not reported from the UK before and the recently described species Vibrio injenensis hitherto only reported from human patients in Korea. Our novel method has the power to detect bacterial pathogens in the environment that potentially pose a serious risk to public health.