Hilly and mountainous regions are significantly impacted by soil erosion, primarily due to rainfall-runoff processes occurring on the hillslope scale. Assessing soil erosion is crucial for quantifying the loss of soil carbon and nutrients, which diminishes the potential of soil ecosystem services and is critical for mitigating the impacts of climate change on food security. However, the Himalayan landscape poses serious challenges for assessing soil erosion due to its steep and rugged terrain, which hinders the use of conventional and modelling methods. The fallout radionuclide—137Cs has been extensively utilized as an environmental marker for investigating soil redistribution processes. Despite its potential, there is a notable lack of 137Cs-based soil erosion studies in the Himalayan region. In this context, we assessed the applicability of the fallout radionuclide—137Cs method in quantifying soil erosion rates and identifying erosion hotspots on two hillslopes of the Higher Himalayas. On the hillslope scale, we observed that soil erosion rates vary based on slope gradient and land use/land cover. Forested areas exhibited the lowest soil erosion rates compared to cultivated areas, while flat hillslope positions experienced lower erosion rates than steeper positions. The average net erosion rate for the Harsil hillslope varied among different hillslope positions, ranging from – 2.9 to – 15.6 t ha−1 yr−1. Similarly, in the Gangnani hillslope, the net erosion rates varied across different positions, ranging from – 5.6 to – 39 t ha−1 yr−1. Our findings confirm that the middle and lower hillslope positions are the most critical source areas with higher soil erosion rates, while hillslope positions with forest cover demonstrate the lowest erosion rates, identified as helpful in controlling soil erosion. The study has demonstrated the applicability of FRN as a soil erosion measurement method in the complex, rugged, and steep terrain of the Himalayas, highlighting the need for targeted conservation efforts to mitigate soil erosion and preserve ecosystem integrity.