Identification Of New Group Research Articles

Analysis of the Effect of Outdoor Thermal Comfort on Construction Accidents by Subcontractor Types

The impact of climate on construction site safety varies significantly depending on subcontractor types due to the diverse nature of workplaces and work methods. This study introduces a novel approach by categorizing construction work according to subcontractor types and assessing accident risk probabilistically through the Physiologically Equivalent Temperature (PET), an outdoor thermal comfort index. Additionally, a Hidden Markov Model (HMM)-based clustering methodology was proposed to classify new groups using PET and accident probability. This study proceeded in the following sequence: (i) collection and classification of data, (ii) PET calculation, (iii) calculation of accident probability, and (iv) clustering and Pearson correlation coefficient analysis. As a result of clustering, each group was classified according to the workplace. Groups 2 and 3 demonstrated a strong positive correlation between accident probability and PET, with correlation coefficients of 0.837 and 0.772, while Group 1 exhibited a moderately positive correlation of 0.474. This study quantitatively evaluated the impact of climate on workers for each subcontractor type using PET, an outdoor thermal comfort index for construction work, and accident probability, resulting in the identification of new groups. The findings of this study may serve as novel benchmarks for safety management in construction worker safety based on PET.

Next-Generation Sequencing for Venomics: Application of Multi-Enzymatic Limited Digestion for Inventorying the Snake Venom Arsenal.

To improve the characterization of snake venom protein profiles, we report the application of a new generation of proteomic methodology to deeply characterize complex protein mixtures. The new approach, combining a synergic multi-enzymatic and a time-limited digestion (MELD), is a versatile and straightforward protocol previously developed by our group. The higher number of overlapping peptides generated during MELD increases the quality of downstream peptide sequencing and of protein identification. In this context, this work aims at applying the MELD strategy to a venomics purpose for the first time, and especially for the characterization of snake venoms. We used four venoms as the test models for this proof of concept: two Elapidae (Dendroaspis polylepis and Naja naja) and two Viperidae (Bitis arietans and Echis ocellatus). Each venom was reduced and alkylated before being submitted to two different protocols: the classical bottom-up proteomics strategy including a digestion step with trypsin only, or MELD, which combines the activities of trypsin, Glu-C and chymotrypsin with a limited digestion approach. The resulting samples were then injected on an M-Class chromatographic system, and hyphenated to a Q-Exactive Mass Spectrometer. Toxins and protein identification were performed by Peaks Studio X+. The results show that MELD considerably improves the number of sequenced (de novo) peptides and identified peptides from protein databases, leading to the unambiguous identification of a greater number of toxins and proteins. For each venom, MELD was successful, not only in terms of the identification of the major toxins (increasing of sequence coverage), but also concerning the less abundant cellular components (identification of new groups of proteins). In light of these results, MELD represents a credible methodology to be applied as the next generation of proteomics approaches dedicated to venomic analysis. It may open new perspectives for the sequencing and inventorying of the venom arsenal and should expand global knowledge about venom composition.

A large-scale genomic snapshot of Klebsiella spp. isolates in Northern Italy reveals limited transmission between clinical and non-clinical settings

The Klebsiella group, found in humans, livestock, plants, soil, water and wild animals, is genetically and ecologically diverse. Many species are opportunistic pathogens and can harbour diverse classes of antimicrobial resistance genes. Healthcare-associated Klebsiella pneumoniae clones that are non-susceptible to carbapenems can spread rapidly, representing a high public health burden. Here we report an analysis of 3,482 genome sequences representing 15 Klebsiella species sampled over a 17-month period from a wide range of clinical, community, animal and environmental settings in and around the Italian city of Pavia. Northern Italy is a hotspot for hospital-acquired carbapenem non-susceptible Klebsiella and thus a pertinent setting to examine the overlap between isolates in clinical and non-clinical settings. We found no genotypic or phenotypic evidence for non-susceptibility to carbapenems outside the clinical environment. Although we noted occasional transmission between clinical and non-clinical settings, our data point to a limited role of animal and environmental reservoirs in the human acquisition of Klebsiella spp. We also provide a detailed genus-wide view of genomic diversity and population structure, including the identification of new groups.

Insurgency and Small Wars: Estimation of Unobserved Coalition Structures

Insurgency and guerrilla warfare impose enormous socio‐economic costs and often persist for decades. The opacity of such forms of conflict is an obstacle to effective international humanitarian intervention and development programs. To shed light on the internal organization of otherwise unknown insurgent groups, this paper proposes two methodologies for the detection of unobserved coalitions of militants in conflict areas. These approaches are based on daily geocoded incident‐level data on insurgent attacks. We provide applications to the Afghan conflict during the 2004–2009 period and to Pakistan during the 2008–2011 period, identifying systematically different coalition structures. Applications to global terrorism data and identification of new groups or shifting coalitions are discussed.

Predicted group II intron lineages E and F comprise catalytically active ribozymes

Group II introns are self-splicing, retrotransposable ribozymes that contribute to gene expression and evolution in most organisms. The ongoing identification of new group II introns and recent bioinformatic analyses have suggested that there are novel lineages, which include the group IIE and IIF introns. Because the function and biochemical activity of group IIE and IIF introns have never been experimentally tested and because these introns appear to have features that distinguish them from other introns, we set out to determine if they were indeed self-splicing, catalytically active RNA molecules. To this end, we transcribed and studied a set of diverse group IIE and IIF introns, quantitatively characterizing their in vitro self-splicing reactivity, ionic requirements, and reaction products. In addition, we used mutational analysis to determine the relative role of the EBS-IBS 1 and 2 recognition elements during splicing by these introns. We show that group IIE and IIF introns are indeed distinct active intron families, with different reactivities and structures. We show that the group IIE introns self-splice exclusively through the hydrolytic pathway, while group IIF introns can also catalyze transesterifications. Intriguingly, we observe one group IIF intron that forms circular intron. Finally, despite an apparent EBS2-IBS2 duplex in the sequences of these introns, we find that this interaction plays no role during self-splicing in vitro. It is now clear that the group IIE and IIF introns are functional ribozymes, with distinctive properties that may be useful for biotechnological applications, and which may contribute to the biology of host organisms.

Genotype Analysis and Phenotypic Manifestations of Children With Intermediate Sweat Chloride Test Results

Cystic fibrosis (CF) is one of the most common inherited diseases among whites. Since the cloning of the CF transmembrane conductance regulator (CFTR) gene, a number of studies have focused on associations between the genotype and phenotype in CF. This had led to the progressive identification of new groups of patients, including those who have mild lung disease and those who have normal sweat chloride values (< 60 mEq/L). The aim of the present work was to provide information on the genotype and the phenotypic characteristics of children with intermediate-range sweat chloride test results. We focused on children referred to the pulmonary department for various types of pulmonary disease and who had several sweat chloride test results with median values in the range of 40 to 60 mEq/L. Twenty-four patients over a 10-year period were enrolled (mean age, 4.8 years). Respiratory manifestations at initial evaluation included recurrent bronchitis, wheezing, chronic cough, and pneumonia. The duration of the follow-up ranged from 0.5 to 10.5 years. Sputum cultures revealed the presence of Haemophilus influenzae (10 children), Staphylococcus aureus (4 children), and Pseudomonas aeruginosa (3 children). Pancreatic insufficiency was found in two patients. Analysis of the entire coding sequence allowed identification of 16 known mutations in CFTR gene. Fifteen chromosomes (31.2%) carried a mutation in CFTR gene and one allele carried two mutations. Three patients were homozygous or double heterozygous (DeltaF508/DeltaF508, DeltaF508/3849 + 10 kb C-->T, S1235R/G551D). The 5-thymidine allele was identified in four children. These results indicate an higher frequency of CFTR gene mutations in patients with borderline sweat chloride test results, compared to data reported in the general population. They lead to the recommendations for complete pulmonary and GI investigations in this group of patients, as well as assiduous care and medical follow-up.

Further studies on the serological relationships of group C arthropod-borne viruses and the application of these relationships to rapid identification of types.

Summary In studies by HI and CF tests of the serological reactions of 200 strains of group C virus, 199 strains reacted similarly to one of the six prototype viruses; examples are given of HI and CF reaction patterns for each type. The one exception, strain H 5546, reacted by HI like Caraparu and by CF like Oriboca and Murutucu. These serological relationships have suggested a method of combined CF and HI techniques for the rapid identification of new group C isolates. Since 1959, 576 isolates have been identified to type by this method.