Abstract. Over the past decades, observations of buried glacier ice exposed in coastal bluffs and headwalls of retrogressive thaw slumps of the Arctic have indicated that considerable amounts of late Pleistocene glacier ice survived the deglaciation and are still preserved in permafrost. In exposures, relict glacier ice and intrasedimental ice often coexist and look alike but their genesis is strikingly different. This paper aims to present a detailed description and infer the origin of a massive ice body preserved in the permafrost of Bylot Island (Nunavut). The massive ice exposure and core samples were described according to the cryostratigraphic approach, combining the analysis of permafrost cryofacies and cryostructures, ice crystallography, stable O-H isotopes and cation contents. The ice was clear to whitish in appearance with large crystals (cm) and small gas inclusions (mm) at crystal intersections, similar to observations of englacial ice facies commonly found on contemporary glaciers and ice sheets. However, the δ18O composition (-34.0±0.4 ‰) of the massive ice was markedly lower than contemporary glacier ice and was consistent with the late Pleistocene age ice in the Barnes Ice Cap. This ice predates the aggradation of the surrounding permafrost and can be used as an archive to infer palaeo-environmental conditions at the study site. As most of the glaciated Arctic landscapes are still strongly determined by their glacial legacy, the melting of these large ice bodies could lead to extensive slope failures and settlement of the ground surface, with significant impact on permafrost geosystem landscape dynamics, terrestrial and aquatic ecosystems and infrastructure.