ICAM-1 Concentrations Research Articles

Cytokine profile in autosomal dominant polycystic kidney disease.

To elucidate the pathogenetic mechanism of renal parenchymal injury in autosomal dominant polycystic kidney disease (ADPKD) patients, typically characterized by renal cystic changes paralleled by interstitial inflammation and gradual fibrotic changes, the role of selected inflammatory mediators was evaluated in a group of ADPKD patients with normal glomerular filtration rate. The plasma concentrations of IL-6, IL-8, ICAM-1 and VCAM-1 (which may reflect systemic response to inflammation/infection) were increased in the ADPKD patient group. Coupled with decreased urinary excretion of the IL-1 receptor antagonist (which exerts an anti-inflammatory role), these results suggest that even in overt infection free status, the proinflammatory system is more activated and anti-inflammatory defence system weakened in ADPKD subjects. Our data support the current view that cytokines are candidate contributors to pathogenesis of ADPKD.

Increased soluble ICAM-1 concentration and impaired delayed-type hypersensitivity skin tests in patients with chronic liver disease.

AIMS/BACKGROUND: Soluble ICAM-1 may act as an antagonist of the membrane bound form, which is essential for the adhesion of leucocytes to endothelial cells. The aim of this study was...

Elevated levels of soluble ICAM‐1 in serum of patients with acute myeloid leukemia undergoing bone marrow transplantation

Serum soluble ICAM-1 concentrations were measured in 10 patients with or without chronic graft-vs.-host disease (GVHD) after allogeneic bone marrow transplantation. The serum soluble ICAM-1 levels in the patients with chronic GVHD were significantly higher than that in the patients without chronic GVHD. The data indicated that serum soluble ICAM-1 is a useful parameter for predicting chronic GVHD. © 1996 Wiley-Liss, Inc.

Increase in shedding of intercellular adhesion molecule-1 in human malignant melanoma cell lines treated with hyperthermia in vitro.

Human malignant melanoma cell lines were found to increase shedding of soluble intercellular adhesion molecule-1 (sICAM-1) into the culture medium when the cells were treated with hyperthermia at 41-43 degrees C for 3-6 hr in vitro. The content of ICAM-1 in the cell lysate was also found to be increased after hyperthermia. The increased rate of ICAM-1 concentration in the cells was at maximum when they were incubated at 41 degrees C for 3 hr. Also, the melanoma cell lines heat-treated at 41 degrees C showed more intense immunofluorescence in the ICAM-1 expression on the cell surface. It remains to be investigated further whether the effects of hyperthermia on the ICAM-1 expression in melanoma cells is to augment membrane ICAM-1 expression, which in turn leads to shedding of soluble ICAM-1 or only to acceleration of shedding of sICAM-1 by unknown mechanisms.

238 IL-8 and soluble ICAM-1 (sICAM-1) concentration in bronchoalveolar lavage (BAL) of asthmatic children and infantile wheezers

238 IL-8 and soluble ICAM-1 (sICAM-1) concentration in bronchoalveolar lavage (BAL) of asthmatic children and infantile wheezers

Relevance of ICAM-1 to alcoholic liver cirrhosis.

An elevated serum concentration of soluble intercellular adhesion molecule-1 (sICAM-1) was observed in patients with alcoholic liver cirrhosis in strong correlation with the current activity of the liver disease and the history of alcohol consumption. Circulating ICAM-1 levels were significantly elevated in the sera of all of 25 patients (1,400 +/- 850 ng/ml) compared with normal healthy subjects. The highest levels of sICAM-1 (2,650 +/- 560 ng/ml) were measured in those patients who suffered from superimposed acute alcoholic hepatitis (SAH). Regularly drinking cirrhotic patients without SAH also exhibited significantly higher results than those who had stopped drinking. The serum ICAM-1 concentration showed a negative correlation with the duration of abstinence. No elevated sICAM-1 was found in ascitic fluid of cirrhotic patients, which tends to discount a significant intraperitoneal production of this molecule. flow cytometric analysis revealed an increased expression of ICAM-1 molecules on peripheral blood monocytes of cirrhotic patients. Proliferating bile ducts and parenchymal cells of cirrhotic livers displayed a positive ICAM-1 immunohistochemical reaction in 3 autopsy cases. It is concluded that the elevated levels of sICAM-1 in the serum of patients with alcoholic liver diseases may provide useful diagnostic or prognostic information. However, determination of the exact source of circulating ICAM-1 in patients with alcoholic cirrhosis still demands further investigations.

Serum concentrations of E-selectin, P-selectin, ICAM-1 and interleukin 6 in acute leukaemia patients with chemotherapy-induced leucopenia and bacterial infections.

Serum concentrations of E-selectin (CD62E), P-selectin (CD62P), ICAM-1 (CD54) and interleukin 6 were investigated in acute leukaemia patients with chemotherapy-induced leucopenia and complicating bacterial infections. Serum concentrations of both E-selectin and P-selectin were decreased in the leucopenic patients without infections when compared with levels before chemotherapy; and serum concentrations of both E-selectin and P-selectin showed a further decrease during complicating bacterial infections. In contrast to the leukaemia patients, previously healthy individuals with meningococcal disease showed markedly elevated serum concentrations of E-selectin and normal levels of P-selectin during infection. Serum concentrations of ICAM-1 and interleukin 6 increased during bacterial infections in the acute leukaemia patients with chemotherapy-induced leucopenia. The alterations in serum concentrations of soluble adhesion molecules and interleukin 6 reversed when clinical signs of bacterial infections resolved during antibiotic therapy. Our results demonstrate that acute leukaemia patients with chemotherapy-induced cytopenia show altered levels of both soluble adhesion molecules and interleukin 6 during complicating bacterial infections.

インスリン非依存型糖尿病患者における血清Intercellular Adhesion Molecule-1 (ICAM-1)濃度におよぼすイコサペント酸エチルエステルの効果

Serum ICAM-1 concentraions were measured in 51 patients with non-insulin-dependent diabetes mellitus (NIDDM), and patients with serum ICAM-1 levels higher than the average value were selected. These patients were divided into two groups matched for age, sex duration of diabetes mellitus, HbA1C, and serum ICAM-1 level. We administered 900mg or 1, 800mg of eicosapentanoic acid ethyl ester (EPA-E) for three months to one group while the other group served as the control group.Serum ICAM-1 concentrations were significantly higher in diabetic patients. After three months of administering EPA-E, there were no differences in blood pressure, serum total cholesterol, serum triglyceride, serum type IV collagen, and urinary albumin-creatinie ratio.The changes of serum ICAM-1 values in the EPAE group (n=9) were significantly lower than in the control group (n=10).These results suggest that the serum concentration of ICAM-1 is increased in patients with NIDDM, and that EPA-E may lower the serum ICAM-1 level.

HIV type 1-specific cytotoxic T lymphocytes stimulate HLA class I and intercellular adhesion molecule type 1 expression and increase beta 2-microglobulin levels in vitro.

Besides acting in a direct manner, cytolytic HIV-1-specific CTLs release a variety of cytokines. To assess the potential role of cytokines released by these CTLs we tested the ability of soluble products secreted by HIV-1-specific CTLs to induce HLA class I and ICAM-1 expression and to raise beta 2-microglobulin (beta 2M) concentrations in cell culture. To this end, supernatants were derived from HIV-1-specific CTLs incubated with autologous B lymphoblasts presenting either the cognate HIV-1 epitope or a control peptide. Cell lines and peripheral blood mononuclear cells (PBMCs) were incubated with these supernatants for 24-48 hr. Similarly, cells were cocultured with CTLs and their targets. This study demonstrates that in parallel with lysis of their cognate target, HIV-1-specific CTLs secreted products that stimulated HLA class I and ICAM-1 expression on cell lines and PBMCs. As few as 1000 CTLs significantly induced the expression of these molecules. In addition, secreted products of HIV-specific CTLs enhanced beta 2M release by PBMCs and Jurkat cells. These effects were mediated primarily by IFN-gamma and suggest that HIV-specific CTLs may contribute to increased HLA class I expression in infected tissue and elevated ICAM-1 and beta 2M concentrations in serum and cerebrospinal fluid of infected individuals.

RS 52 Evaluation of the concentrations of ICAM-1 in healthy and inflamed human dental pulp

RS 52 Evaluation of the concentrations of ICAM-1 in healthy and inflamed human dental pulp

Changes in leukocyte counts and soluble ICAMI and E-selectin during cardioputmonary bypass in children

A consequence of cardiopulmonary bypass (CPB) in young children is postoperative capillary leak and associated pulmonary dysfunction. Neutrophils sequester in itic lungs during CPU and may contribute to functional endothelial damage. Endothelial adhesion molecules E-selectin and ICAMI mediate sequential steps in adhesion by binding to leukocyte ligands. Circulating forms of these proteins have recently been identified. We therefore studied changes in the plasma concentrations of soluble E-selectin and soluble ICAMI using fixed phase immunoassays, and associated leukocyte counts in 10 pediatric patients undergoing CPB for corrective cardiac surgery. Preoperative concentrations of soluble E-selectin and soluble ICAM-1 consistently fell during CPB from 89±27ng/ml (mean ± 2SE) and 218±70ng/ml respectively, to 39±12ng/ml and 84 ± 28ng/ml respectively at the beginning of maximum hypothermia during CPB, The haemodilution that occurred during CPB largely explained this fall, but not a more marked decrease in while cell counts that also occurred over this period (6.66±1.23 to 1.73±1.73×109/l) which may reflect increased leukocyte sequestration. By 24 hours postoperatively, levels of both soluble adhesion molecules approached preoperative concentrations, as did lymphocyte counts. In marked contrast neutrophil counts rose appreciably at the end of CPB and during llie immediate postoperative period and remained at these elevated levels 24 hours later. Major consistent elungcs in circulating leukocyte numbers which occur early in CPB may reflect changes in adhesion to endothelium and consequent sequestration. Alterations in the levels of soluble adhesion proteins may influence these processes.

Soluble ICAM-1 serum levels in multiple sclerosis and viral encephalitis.

Intercellular adhesion molecule ICAM-1 has a crucial role in the induction of an immune response and is instrumental in migration of T cells into inflamed tissue. We studied soluble ICAM-1 concentrations in patients with multiple sclerosis (MS), viral encephalitis, and other immunologic diseases, and compared results with those in other noninflammatory, nondemyelinating neurologic disorders as well as in healthy controls. MS patients with clinically active disease or enhancing lesions on MRI had elevated serum levels of soluble ICAM-1. Concentrations of soluble ICAM-1 were also increased in some patients with viral encephalitis. These findings raise the possibility that circulating ICAM-1 serves as a marker of acute inflammatory events in the brain and add to evidence implicating this adhesion molecule in the pathogenesis of MS.

Serum ICAM-1 concentrations in patients with psoriasis treated with antithyroid thioureylenes

Serum concentrations of intercellular adhesion molecule-1 (ICAM-1), a marker of early T-cell activation were measured in 14 patients with stable plaque psoriasis who received treatment for 8 weeks with the antithyroid thioureylenes, propylthiouracil (PTU) or methimazole (MMI) which have been previously shown to produce significant improvement in such patients. Baseline serum concentrations of ICAM-1 were significantly higher in the patients with psoriasis compared with normal control volunteers. Following therapy with either PTU (300 mg daily) or MMI (40 mg daily) serum ICAM-1 concentrations did not decline significantly. Since ICAM-1 expression on vascular endothelium increases in active psoriasis, and is postulated to promote T-cell migration to and retention at these sites, it is hypothesized that the beneficial therapeutic effects of thioureylenes in psoriasis occur distal to the events that lead to lymphocyte migration to vascular structures in the dermis.

Assay of vitamin B 12 .

The increase in production of Multi-Walled Carbon Nanotubes (MWCNTs) goes along with growing concerns about health risks. Few, rather small, studies have reported biological effects of MWCNTs in humans including increased concentrations of cardiovascular markers fibrinogen, ICAM1 and IL-6, but findings are largely inconsistent. The objective of this study was to assess the association between occupational exposure to MWCNTs and biomarkers of cardiovascular risk. A cross-sectional biomarker study was performed among workers of a company commercially producing flexible MWCNTs and a matched unexposed population. 12 cardiovascular markers were measured in participants’ blood (phase 1). In a sub-population these measures were repeated after 5 months (phase 2). We analysed associations between MWCNT exposure and biomarkers of cardiovascular risk, corrected for age, BMI, sex and smoking. 22 exposed and 42 unexposed workers were included in phase 1 and a subgroup of 13 exposed workers and 6 unexposed workers in phase 2 of the study. Both in phase 1 and phase 2 we observed an upward trend in the concentration of endothelial damage marker ICAM-1, with increasing exposure to MWCNTs. This finding is supported by significantly elevated monocyte counts among the same workers. No significant associations were found between exposure to MWCNTs and the other cardiovascular markers tPA, Fibrinogen, VCAM-1, IL-6, E selectin, TNF-α and D-Dimer. The results of the present study should be viewed as explorative and requires confirmation in larger studies. Our results for ICAM-1 point towards a potential for endothelial damage due to exposure to MWCNT.