IA Column Research Articles

Activated sepharose as an adsorbent for antibodies of foot-and-mouth disease virus, and its use as an immunoadsorbent for type and subtype differentiation.

Immunoadsorbents were prepared by coupling antibodies of foot-and-mouth disease virus (FMDV) to activated Sepharose. The coupled antibodies retained their capacity to adsorb foot-and-mouth disease virus. FMDV type and subtype differentiation could be performed by the use of antibody-immunoadsorbents (IA). Type differentiation was demonstrated with FMD A-, O-, and C-virus. For subtype differentiation A2 and A3 strains were used. Subtype differentiation demands careful attention to certain factors, the best results being obtained by using IA columns from preparations with 2–10 mg protein of a globulin fraction containing FMDV antibodies coupled to 100 mg activated Sepharose, undiluted virus cell culture fluids (105 to 106 PFU/ml), and a flow rate of 10 to 20 ml/h. Results obtained by neutralizing tests and by the present experiments have been briefly discussed.