Hyporheic Research Articles

Neglected area of meiobenthos: bibliometric analysis in the status of research on hyporheic meiofauna

Studies on freshwater meiofauna are still enigmatic. This field of research is relatively neglected because this topic is not as increasing as the others, creating a gap in this area. The deficit of knowledge and surveys impose a barrier on new research and researchers to emerge and on the increase of scientific interest in this area. This paper contains a brief review of what already exists on freshwater meiofauna worldwide, focusing on increasing knowledge on this topic and making this little-known area of meiofauna a common study field in science. The methodology involved a bibliographic survey from the Web of Science (WOS) bibliographic database. The search was performed by document title, keywords, and abstract, highlighting meiofauna (or meiobenthos) and hyporheic zone (or hyporheos). The results showed 28 documents on hyporheic meiofauna worldwide, mainly concentrated in Germany. The few articles highlighted that the lack of research on the hyporheic meiofauna community, its ecology, taxonomy, and biology is evident. It is hoped that this bibliometric review can be used as an alert about this area of meiofauna that is so important but at the same time is neglected, having a very exclusive group of authors and works, being of extreme importance different directions of research that involves hyporheic meiofauna in order to a better understanding of this fauna importance, ecosystemic services and ecology.

Low hyporheic denitrification in headwater streams revealed by nutrient injections and in situ gas measurements

Stream networks can retain or remove nutrient pollution, including nitrate from agricultural and urban runoff. However, assessing the location and timing of nutrient uptake remains challenging because of the hydrological and biogeochemical complexity of dynamic stream ecosystems. We used a novel approach to continuously characterize the biological activity in a stream with in situ measurement of dissolved gases by membrane inlet mass spectrometry (MIMS). In a headwater stream in western France, we compared in situ measurements of O2, CO2, N2, and N2O (the main gases associated with respiration, including denitrification) with more traditional laboratory incubations of collected sediment. The in situ measurements showed near-zero denitrification in the stream and the hyporheic zone. However, the laboratory incubations showed a low but present denitrification potential. This demonstrates how denitrification potential is not necessarily expressed in field hydrological and geochemical conditions. In situ measurements are thus crucial to quantify expressed rates of nutrient removal. Broader application of in situ gas measurement based on technologies such as MIMS could enhance our understanding of the spatiotemporal distribution of stream and hyporheic processes and overall nutrient retention at stream network scales.

Turbulence‐Driven Clogging of Hyporheic Zones by Fine Particle Filtration

AbstractHyporheic exchange (HE), fine particle deposition and clogging are tightly coupled processes that control ecosystem services in rivers. While HE is assumed to be induced primarily by riverbed topography, surface flow turbulence also drives significant exchange. We show that turbulence‐driven HE produces large interfacial fluxes and drives long‐term feedback between HE and fine suspended particles via bed clogging. Turbulence significantly increases total HE fluxes as it rapidly delivers suspended particles into porewater over the entire interface, whereas advective pumping exchange only delivers particles into focused downwelling regions on the upstream side of bedforms. While turbulence is associated with rapid fluctuations and shallow HE, it is key on longer‐timescale outcomes, namely bed clogging. However, beyond the general effect of clogging in attenuating HE, turbulence‐driven HE will also be important for other river‐borne materials that are retained and transformed within hyporheic zones, such as nutrients and organic pollutants.

With spatial distribution, risk evaluation of heavy metals and microplastics to emphasize the composite mechanism in hyporheic sediments of Beiluo River

This study aimed to assess the hazardous impacts of heavy metals (HMs) enrichment on the surface of microplastics (MPs) in the hyporheic zone. The present work analyzed the spatial distribution and risk evaluation of HMs (V, Cr, Mn, Co, Ni, Cu, Zn, As, Cd, and Pb) and MPs and the mechanism of HMs enrichment on MPs in the sediments. The highest rates of contamination were for Cd, Pb, and As. The main types of MPs were fiber, blue, and a size smaller than 500 µm. The lower reaches of the Beiluo River had the most serious HMs and MPs pollution, especially BL-10 (HMs: CF-Cd, 41.91; EF-Cd, 50.87; Igeo-Cd, 4.80; RI, 1291; PN, 29.83; MPs: abundance, 890 ± 18 items/kg). Meanwhile, the principal component analysis showed that natural, industrial activities, and agricultural production and transportation were primary HMs sources in sediments, and Cd, Co, and Pb were the main enriched metals on the surface of MPs. More importantly, regarding the interaction mechanism of these composite pollutants, we concluded that electrostatic adsorption and biofilm mediation were the main mechanisms of the synergistic effect. Overall, our findings provide a theoretical basis for further research on the ecotoxicity of composite pollutants in aquatic environments.

Mechanism of sinuosity effect on self-purification capacity of rivers.

As one of the important characteristics of river morphology, river sinuosity has a direct impact on the river water quality and self-purification capacity. In the present study, 4 physical river channel simulation models using circulating water with a sinuosity of 2.2, 1.8, 1.4, and 1.0, respectively, were established in our laboratory. Related hydraulic tests and detection were performed, including the detection of microbial communities in overlying water, monitoring of the river flow velocity and depth, and observation of the river flow line and bank scouring. The results show that the TN reduction rate at a sinuosity of 2.2 was 1.09, 1.20, and 1.75 times that at a sinuosity of 1.8, 1.4, and 1.0, respectively. And the total plate count for the set of tests with a sinuosity of 2.2 was 3.32 times that for the set of tests with a straight channel. The sinuous rivers have more complex flow regimes, more suitable hydraulic conditions, larger hyporheic zone areas, better microbial environments, and longer river flow paths, giving them a higher purification capacity against pollution. These findings can provide a theoretical basis for the optimization of water system layout and the restoration of river environments in the process of urbanization in China.

Rethinking Aerobic Respiration in the Hyporheic Zone under Variation in Carbon and Nitrogen Stoichiometry.

Hyporheic zones (HZs)─zones of groundwater-surface water mixing─are hotspots for dissolved organic matter (DOM) and nutrient cycling that can disproportionately impact aquatic ecosystem functions. However, the mechanisms affecting DOM metabolism through space and time in HZs remain poorly understood. To resolve this gap, we investigate a recently proposed theory describing trade-offs between carbon (C) and nitrogen (N) limitations as a key regulator of HZ metabolism. We propose that throughout the extent of the HZ, a single process like aerobic respiration (AR) can be limited by both DOM thermodynamics and N content due to highly variable C/N ratios over short distances (centimeter scale). To investigate this theory, we used a large flume, continuous optode measurements of dissolved oxygen (DO), and spatially and temporally resolved molecular analysis of DOM. Carbon and N limitations were inferred from changes in the elemental stoichiometric ratio. We show sequential, depth-stratified relationships of DO with DOM thermodynamics and organic N that change across centimeter scales. In the shallow HZ with low C/N, DO was associated with the thermodynamics of DOM, while deeper in the HZ with higher C/N, DO was associated with inferred biochemical reactions involving organic N. Collectively, our results suggest that there are multiple competing processes that limit AR in the HZ. Resolving this spatiotemporal variation could improve predictions from mechanistic models, either via more highly resolved grid cells or by representing AR colimitation by DOM thermodynamics and organic N.

Fuzzy-logic indicators for riverbed de-clogging suggest ecological benefits of large wood

Rivers provide dynamic habitats with ecological niches, particularly in their mobile sand, gravel, and cobble riverbed patches that create an active hyporheic zone. Natural or artificial deposition of fine sediment may clog the porous matrix of the hyporheic zone, impairing exchange processes between the subsurface and surface water. Clogging reduces the permeability of the sediment matrix, thus degrading the ecological functionality of the hyporheic zone. Once clogged, the ecological functions may be recovered through active stream restoration, which requires considerate site assessment. To this end, clogging is typically assessed by expert opinion of substrate characteristics including grain size characteristics, porosity, hydraulic conductivity, and interstitial oxygen content. To overcome limitations of expert assessment, such as subjectivity expressed in noisy decision-making, this study introduces a novel fuzzy-logic method based on physically sound rules. The method provides quantitative indicators for clogging and declogging to evaluate the effectiveness of stream restoration. We applied the fuzzy-logic method to test whether the placement of large wood, a common restoration practice, can locally prevent or reduce clogging. Two measurement series from before and after a morphologically effective flood suggested that large wood placements perpendicular to the flow generate elevated amounts of declogging. The tested logs caused a greater amount of declogging within their region of influence than observed at a reference point. The effect was stronger for a log emergent at baseflow. The declogging assessment showed that the novel fuzzy-logic indicators can reasonably overcome subjective judgment by accounting for multi-variate quantitative changes rather than individual parameter trends.

Two New Species of Nipponbathynella Schminke, 1973 from South Korea, with a Brief Discussion on Korean Parabathynellid Genera (Crustacea, Bathynellacea)

Parabathynellidae is a family well known for its diversity and is endemic to the interstitial hyporheic zone of South Korea. Two new parabathynellid species, Nipponbathynella yeongchunensis sp. nov. and N. gapcheonensis sp. nov., were found through groundwater sampling on riverbanks in South Korea. Nipponbathynella yeongchunensis sp. nov. can be distinguished from its congeners by the base socket of the two dorsal setae on the antennule’s first segment. Nipponbathynella gapcheonensis sp. nov. can be distinguished by the protopod of thoracopod II–IV with hairs. We provide morphological descriptions and remarks about the two new species with molecular information based on 18S rRNA and partial COI gene sequences. In addition, phylogenetic analysis, including Nipponbathynella Schminke, 1973, which had not been previously molecularly evaluated, was inferred. The results of the 18S rRNA gene phylogeny suggest a close relationship between the genera Nipponbathynella and Arisubathynella, as well as Europe–North American genera. We have also included a brief discussion and an identification key for the five known parabathynellid genera found in South Korea.

Effects of boundary hydraulics, dissolved oxygen, and dissolved organic carbon on growth and death dynamics of aerobic microbes in riverbed dune-induced hyporheic zones

Surface and groundwater interact in the hyporheic zone beneath and adjacent to rivers in the presence of a diverse microbial community. Heterotrophic bacteria mediate a range of environmentally important reactions, yet few studies have quantified bacterial growth and death dynamics in the hyporheic zone, and none have systematically analyzed their response to variations in hydraulic or chemical conditions. We used MODFLOW and SEAM3D to simulate hydraulics; dissolved oxygen (DO) and dissolved organic carbon (DOC) transport; and aerobic microbial metabolism, growth, and death in hyporheic zones induced by riverbed dunes. We ran simulations both with and without growth/death processes, and varied hydraulic parameters and DO/DOC boundary concentrations. Microbial biomass reached steady state (t = 3 days) in every simulation, at which time there was greater biomass and DOC biodegradation rates in the hyporheic flowcell (300 % and 85 % higher for the base case, respectively) when accounting for microbial growth dynamics. This occurred as microbial biomass tailored its spatial distribution to the availability of DO and DOC, demonstrating the importance of simulating growth/death processes. Biomass generally increased with hyporheic flow cell area as upwelling groundwater decreased. When varying surface water DO and DOC source concentrations relative to the base case, the greatest effect on biomass occurred when increasing DOC and decreasing DO. We determined minimum DO and DOC steady-state concentrations required for microbial growth, but the minimums were not absolute or related by stoichiometry. Increasing DOC created a smaller area of microbes with higher concentrations relative to the base case. Increasing DO slightly increased the area occupied by microbes while keeping the total biomass nearly constant. Overall, microbial growth and death dynamics depend on DO and DOC availability in the hyporheic zone, which is dependent on DOC/DO boundary concentrations and hyporheic flow paths, and in turn the hydraulic interaction between surface water and groundwater.

Effects of regional groundwater discharge on transverse hyporheic exchange in compound channels

Previous studies of transverse hyporheic exchange in compound rivers mainly focused on processes driven by riverbed morphology, and neglected the effects of regional groundwater discharge (RGD). This study numerically investigated the transverse hyporheic exchange subjected to the joint influences of bedform and RGD in a conceptual compound channel. The results show the existence of an interfacial exchange zone (IEZ) across compound riverbeds even in the presence of RGD. A stagnation zone forms at the intersection of hyporheic flow and discharging groundwater flow when RGD flux is large, implying localized inactive nutrient processing within this zone. RGD sharply reduces the scale of IEZ whereas the flow rate of IEZ decreases slightly, consequently resulting in shorter residence times. The flux, depth and mean residence time of IEZ linearly, exponentially and exponentially decrease with RGD, respectively. RGD is likely to hinder solute transport depth and mass accumulation through IEZ depth and control transfer rate by mean residence time of IEZ. Therefore, a larger RGD would decrease the maximum solute transport depth, accompanied by less amount accumulation and a lower transfer rate in the bed. For a given bedform, a power model can describe how solute amount accumulation depends on hyporheic fluxes, while the solute accumulation dependence can be described by a linear model when the RGD condition is fixed. Findings from this study would help better understand the various mechanisms underlying hyporheic zones in compound channels.

Investigations on microplastic infiltration within natural riverbed sediments

Several studies focused on the role of rivers as vectors of microplastics (MPs) towards the sea. It is well known that during their path through the fluvial environment, MPs interact with riverbed sediments; however, the main factors impacting the mobility of MPs within the upper part of the hyporheic zone are not clear yet. The present work investigates the role of different sediment size layers in affecting the mobility of the most common MP (Polyethylene terephthalate - PET - spheres, PET 3D-ellipsoids, polystyrene - PS - fragments and polyamide - PA - fibers) within sediment porous media under different hydraulic loads (HL) and time scales (t) conditions. Results indicated the relationship between the characteristic MP diameter and that of the grains as the main parameter for the MP infiltration into the sediment layer. The maximum infiltration depth was found to not depend on HL and t. However, HL was able to influence the percentage of MPs penetrating the superficial layer and their distribution within the first 10–15 cm of the sediment layer. None of the MPs were found at depths >20–25 cm, where only PET spheres were detected. Starting from the suffusion theory, a model able to predict the MP maximum infiltration depth in the range of parameter values was provided.The outcome indicates the importance of considering geometrical and hydrodynamic aspects of the riverbed sediment layer to better characterize the spatial and temporal scales of MP transport in freshwater environments.

Hyporheic exchange and nitrogen cycle processes under the dual effects of flooding and heterogeneous streambed

In this study, we investigated the effects of media heterogeneity and flood processes on hyporheic exchange and nitrogen cycle processes in hyporheic zone adjacent to a natural in-stream gravel bar. We conducted field experiments and set a reactive transport model of surface-groundwater exchange based on the monitoring results. The flood process is obtained based on field monitoring, and the heterogeneous medium field is constructed using the SAR3DC method based on in-site experiment results. The results showed that both the heterogeneity of the medium and the flood process can enhance the spatial variability of velocity distribution, resulting in an increase of exchange flux, a decrease in lag time, and an extension of the migration path. For heterogeneous media, it is easier to form specific channels for high velocity flow, and the flow velocity within them is more sensitive to changes in media and hydrodynamic conditions, the direction of hyporheic exchange can be reversed in some specific channels. For the nitrogen cycle processes, the heterogeneous medium condition determines the source-sink functions and the direction of nitrate nitrogen reduction in hyporheic zone by providing a wider reaction space for reactants, reducing the lag time to 1/40 and increasing the migration path by 4 times. The flood process can increase the infiltration amounts of reactants by 5 times and reduce the lag time (time of particle entry into the hyporheic zone to migrate out) to 1/6. In general, under the effects of media heterogeneity and flood processes, the reaction rates and nitrate nitrogen reduction rates during the nitrogen cycle processes are decreasing and the hyporheic zone will face greater pollution risks.

The competition between heterotrophic denitrification and DNRA pathways in hyporheic zone and its impact on the fate of nitrate

Dissimilatory nitrate reduction to ammonium (DNRA) and heterotrophic denitrification (DEN) are recognized as two key nitrate reduction pathways competing for both nitrate and dissolved organic carbon (DOC). However, the mechanisms and influencing factors governing this competition in the dynamic hyporheic system remain unclear. In this study, a numerical model that coupled flow, reactive solute transport and microbial activities was developed to investigate the competition between DEN and DNRA in the hyporheic zone. The modeling results indicate that DNRA hotspots are distributed along the hyporheic flow path. The greater the flow velocity, the farther the DNRA hotspots are from the water-sediment interface. In the simulation scenario involving river stage fluctuations, the relative contribution of DNRA resulting from these fluctuations under a small initial hydraulic gradient (0.001) of the hyporheic zone increased by 60% compared to the range observed under a large initial hydraulic gradient (0.01). The C/N ratio in river water does not dictate the prevailing microbial group at specific locations within the hyporheic zone. Instead, the river C/N ratio governs the competition outcomes across the entire hyporheic system, demonstrating a three-phase pattern. The nitrate removal efficiency in the hyporheic system approaches 100% at C/N = 3. As the stream C/N ratio increases, the contribution of DNRA gradually rises and can reach 80% (C/N = 10). At this point, the contribution of the heterotrophic denitrifying bacteria and their corresponding nitrate removal efficiency decreases to only about 20%. Disregarding the DNRA reaction might lead to an underestimation of nitrate removal efficiency of a hyporheic zone. Our investigation improves the understanding of nitrate fate in hyporheic zones and provides a scientific foundation for watershed management and pollution control.

Life History Traits of the Stygophilous Amphipod Synurella ambulans in the Hyporheic Zone of the Lower Reaches of the Upper Sava River (Croatia)

Estimating the growth and population dynamics of crustaceans is important for understanding the role of species in ecosystems and for conservation and monitoring purposes. This study investigated temporal and spatial variation in population dynamics (growth rate, instantaneous mortality rates, and longevity) of the stygophilous freshwater amphipod Synurella ambulans (F. Müller, 1846), and influencing environmental factors. Seasonal sampling was conducted from December 2018 to October 2019 at two sampling sites (Jarun and Medsave) along the Sava River in northwestern Croatia. A Bhattacharya cohort analysis was applied to the length-frequency data using the FISAT software package. At least four cohorts were distinguished during the year. The possible influence of environmental factors on growth parameters was investigated using Canonical Correspondence Analysis (CCA), which revealed that the important environmental factors influencing S. ambulans abundance were water temperature and dissolved oxygen concentration. The average total body length (TBL) of females was 4.25 mm, while the TBL of males was 3.34 mm. The asymptotic length (L∞) and growth coefficient (K) were estimated to be 6.30 mm and 0.92 year−1 for females and 5.40 mm and 0.59 year−1 for males, respectively, at the Jarun site. L∞ and K were estimated to be 7.20 mm and 0.34 year−1, respectively, for females and 4.00 mm and 1.20 year−1, respectively, for males at the Medsave site. Estimated total mortality (Z) for each sex had higher values for males than females at both sampling sites. The lack of ecological knowledge on S. ambulans populations and relationships with invertebrate communities in the hyporheic zone of the large river ecosystems emphasises the importance of a detailed study for protecting this species and its vulnerable ecotonal groundwater-connected environment.

Integrated numerical modeling to quantify transport and fate of microplastics in the hyporheic zone.

Despite the significance of rivers and streams as pathways for microplastics (MP) entering the marine environment, limited research has been conducted on the behavior of MP within fluvial systems. Specifically, there is a lack of understanding regarding the infiltration and transport dynamics of MP across the streambed interface and within the hyporheic sediments. In this study, transport and retention of MP are investigated using a new numerical modeling approach. The model is built as a digital twin of accompanying flume experiments, which are used to validate the simulation results. The model accurately represents particle transport in turbulent water flow and within the hyporheic zone (HZ). Simulations for transport and infiltration of 1µm MP particles into a sandy streambed demonstrate that the advection-dispersion equation can be used to adequately represent particle transport for pore-scale sized MP within the HZ. To assess the applicability of the modeling framework for larger MP, the experiment was repeated using 10µm particles. The larger particles exhibited delayed infiltration and transport behavior, and while the model successfully represented the spatial extent of particle transport through the HZ, it was unable to fully replicate hyporheic transit times. This study is the first to combine explicit validation against experimental data, encompassing qualitative observations of MP concentration patterns and quantification of fluxes. By that, it significantly contributes to our understanding of MP transport processes in fluvial systems. The study also highlights the advantages and limitations of employing a fully integrated modeling approach to investigate the transport and retention behavior of MP in rivers and streams.

Effect of channel bed sediment on the transport behaviour of superparamagnetic silica encapsulated DNA microparticles in open channel injection experiments

AbstractRecently, superparamagnetic silica encapsulated DNA microparticles (SiDNAFe) were designed and in various experiments used as a hydrological tracer. We investigated the effect of bed characteristics on the transport behaviour and especially the mass loss of SiDNAFe in open channel injection experiments. Hereto, a series of laboratory injection experiments were conducted with four channel bed conditions (no sediment, fine river sediment, coarse sand, and goethite‐coated coarse sand) and two water qualities (tap water and Meuse water). Breakthrough curves (BTCs) were analysed and modelled. Mass loss of SiDNAFe was accounted for as a first‐order decay process included in a 1‐D advection and dispersion model with transient storage (OTIS). SiDNAFe BTCs could be adequately described by advection and dispersion with or without a first‐order decay process. SiDNAFe mass recoveries exhibited a wide range, varying from 50% to 120% from sediment‐free conditions to coarse (coated) sediment. In 6 out of 8 cases, SiDNAFe mass recovery was complete. Retention of SiDNAFe was 1–2 orders of magnitude greater than gravitational settling rates, as determined in Tang et al. (Hydrological Processes, e14801, 2023). We reason this was due to grain‐scale hyporheic flows and coupled water‐sediment‐particle interactions. The dispersive behaviour of SiDNAFe generally mimicked that of NaCl tracer. We concluded that SiDNAFe can be used in tracing experiments. However, water quality and sediment characteristics may affect the fate of SiDNAFe in river environments. SiDNAFe is a promising tool for particulate multi‐tracing in large rivers.

Water Flow and Heat Transport in the Hyporheic Zone of Island Riparian: A Field Experiment and Numerical Simulation

Water Flow and Heat Transport in the Hyporheic Zone of Island Riparian: A Field Experiment and Numerical Simulation

Thermal insulation versus capacitance: A simulation experiment comparing effects of shade and hyporheic exchange on daily and seasonal stream temperature cycles

AbstractIn streams where water temperatures stress native biota, management of riparian shade or hyporheic exchange are both considered viable management strategies for reducing the peaks of daily and seasonal stream channel temperature cycles. Although shade and hyporheic exchange may have similar effects on stream temperatures, their mechanisms differ. Improved understanding of the heat‐exchange mechanisms influenced by shade and hyporheic exchange will aid in the appropriate application of either stream temperature management strategy. To illustrate a conceptual model highlighting shade as ‘thermal insulation’ and hyporheic exchange imparting ‘thermal capacitance’ to a stream reach, we conducted an in‐silico simulation modelling experiment increasing shade or hyporheic exchange parameters on an idealized, hypothetical stream. We assessed the potential effects of increasing shade or hyporheic exchange on a stream reach using an established process‐based heat‐energy budget model of stream‐atmosphere heat exchange and incorporated an advection‐driven hyporheic heat exchange routine. The model tracked heat transport through the hyporheic zone and exchange with the stream channel, while including the effects of hyporheic water age distribution on upwelling hyporheic temperatures. Results showed that shade and hyporheic exchange similarly damped diurnal temperature cycles and differentially altered seasonal cycles of our theoretical stream. In winter, hyporheic exchange warmed simulated channel temperatures whereas shade had little effect. In summer, both shade and hyporheic exchange cooled channel temperatures, though the effects of shade were more pronounced. Our simple‐to‐grasp analogies of ‘thermal insulation’ for shade effects and ‘thermal capacitance’ for hyporheic exchange effects on stream temperature encourage more accurate conceptualization of complex, dynamic heat exchange processes among the atmosphere, stream channel, and alluvial aquifer.

Energy Status of Stygophilous Amphipod Synurella ambulans as a Promising Biomarker of Environmental Stress in the Hyporheic Zone

The hyporheic zone (HZ) is a sensitive ecotone in river ecosystems because of its biodiversity and susceptibility to human activities. Hyporheic fauna are exposed to multiple stressors that affect the physiology and metabolism of organisms and ultimately ecosystem functioning and biodiversity. This study aimed to quantify adenine nucleotides in the stygophilous freshwater amphipod Synurella ambulans and to evaluate the potential of adenylate energy charge (AEC) as a physiological biomarker of general stress in the HZ of the Sava River at sites with different intensities of environmental and anthropogenic stress. Field studies were conducted seasonally (in December 2018 and April, July, and October 2019) at two sampling sites, one upstream (1-UP) and the other downstream (2-DOWN) of the discharge from the wastewater treatment plant using the standard piston pump. The amphipod population from site 1-UP had significantly higher AEC values than the population from site 2-DOWN in all seasons except summer. Coordinated changes in AEC values with the ATP/ADP ratio indicated differences in energy status between the two populations. However, no changes in the apparent equilibrium constant of adenylate kinase were observed, except in spring at site 1-UP. Multiple linear regression models showed the strongest associations of AEC with Fe and Zn accumulated in amphipods, followed by environmental factors (conductivity, dissolved O2, and concentrations of nitrites and phosphates in the interstitial water). AEC was shown to be a useful index of environmental stress in S. ambulans because it can directly measure the change in available energy and thus the metabolic stress to which the organism is exposed. Finally, seasonal and spatial variations in AEC values reflected ecological status in the HZ.

Mapping Surface Water Presence and Hyporheic Flow Properties of Headwater Stream Networks With Multispectral Satellite Imagery

AbstractGrowth and contraction of headwater stream networks determine habitat extent, and open a window to the hyporheic zone. A fundamental challenge is observation of this process: wetted channel extent is dynamic in space and time, with wetted channel length varying by orders of magnitude over the course of a single storm event in headwater catchments. To date, observational data sets are produced from boots‐on‐the‐ground campaigns, drone imaging, or flow presence sensors, which are often laborious and limited in their spatial and temporal extents. Here, we evaluate satellite imagery as a means to detect wetted channel extent via machine learning methods trained on local surveys of wetted channel extent. Even where channel features are smaller than the imagery's spatial resolution, the presence of surface water may be imprinted upon the spectral signature of an individual pixel. For two catchments in northern California with minimal riparian canopy cover and highly dynamic wetted channel extent, we train a random forest model on RapidEye imagery captured contemporaneously with the existing surveys to predict wetted channel extent (accuracy >91%). The model is used to produce length‐discharge (L‐Q) relations and to calculate spatially distributed estimates of channel hyporheic flow capacity and exchange. A sharp break in hyporheic flow capacity occurs from main stem channels to lower order tributaries, resulting in a stepped L‐Q relationship that cannot be captured by traditionally used power law models. Remotely sensed imagery is a powerful tool for mapping wetted channels at high spatial resolution.