Event Abstract Back to Event A New Murine Model Of Osmotic Demyelination Syndrome Joanna Bouchat1*, Bruno Couturier2, 3, Fabrice Gankam-Kengné2, 3, Guy Decaux2 and Charles Nicaise1 1 Université de Namur, Laboratory Neurodegeneration and Regeneration, URPhym - NARILIS, Belgium 2 Erasme Hospital, Université Libre de Bruxelles, Research Unit for the Study of Hydromineral Metabolism, Department of General Internal Medicine, Belgium 3 Université Libre de Bruxelles, Laboratory of Histology, Histopathology and Neuroanatomy, Belgium Osmotic demyelination syndrome (ODS) is a severe neurological complication occuring after inadvertant rapid correction of chronic hyponatremia. This pathology primarily affects the CNS and is characterized by large areas of demyelination in centropontine or extrapontine regions, often correlated with clinical symptoms : from lethargia to coma or even death. Historically, the most used ODS animal model is Rattus norvegicus, that mimicked myelin and oligodendrocyte loss and allowed important insights in the management and in the understanding of ODS pathology. According to an adapted protocol from the rat model, we developed a murine model of ODS that will help at unravelling the molecular and cellular pathophysiological mechanisms. Chronic hyponatremia was induced in mice using continuous desmopressin infusion and a low-sodium liquid diet. After 4 days, blood sodium dropped to 113±11 mEq/L in the hyponatremic group (compared to normonatremic group 148±1 mEq/L, p<0.001) and animals showed clinical signs of mild lethargia. Hyponatremia was next corrected by a single injection of hypertonic saline solution targeting a normal range of blood sodium. 24 hours after correction, blood natremia was measured to determine the final 24-hours sodium correction gradient (33±3 mEq/L, p<0.001 between hyponatremia and correction of hyponatremia). Interestingly, mice displayed transient increased overall locomotor activity during the first 12 hours post-correction before a drastic worsening of neurological symptoms, most likely due to occurrence of demyelination. Histopathologically, ODS mice showed large areas of demyelination in subcortical, midbrain regions and in the pons, attested by loss of myelin in Eriochrome R staining and anti-myelin binding protein immunohistochemistry. In conclusion, we successfully translated the rat model of ODS to a mouse model and reproduced the neurological as well as the histological outcomes observed in human cases of ODS. Keywords: Osmotic Demyelination Syndrome, chronic hyponatremia, demyelination, mouse model, histopathological analysis Conference: Belgian Brain Council 2014 MODULATING THE BRAIN: FACTS, FICTION, FUTURE, Ghent, Belgium, 4 Oct - 4 Oct, 2014. Presentation Type: Poster Presentation Topic: Basic Neuroscience Citation: Bouchat J, Couturier B, Gankam-Kengné F, Decaux G and Nicaise C (2014). A New Murine Model Of Osmotic Demyelination Syndrome. Conference Abstract: Belgian Brain Council 2014 MODULATING THE BRAIN: FACTS, FICTION, FUTURE. doi: 10.3389/conf.fnhum.2014.214.00017 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Jun 2014; Published Online: 27 Jun 2014. * Correspondence: Miss. Joanna Bouchat, Université de Namur, Laboratory Neurodegeneration and Regeneration, URPhym - NARILIS, Namur, Belgium, joanna.bouchat@unamur.be Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Joanna Bouchat Bruno Couturier Fabrice Gankam-Kengné Guy Decaux Charles Nicaise Google Joanna Bouchat Bruno Couturier Fabrice Gankam-Kengné Guy Decaux Charles Nicaise Google Scholar Joanna Bouchat Bruno Couturier Fabrice Gankam-Kengné Guy Decaux Charles Nicaise PubMed Joanna Bouchat Bruno Couturier Fabrice Gankam-Kengné Guy Decaux Charles Nicaise Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.