HomeCirculation ResearchVol. 24, No. 4Reduced Cardiac Myosin Adenosinetriphosphatase Activity in Dogs with Spontaneously Occurring Heart Failure Free AccessResearch ArticlePDF/EPUBAboutView PDFSections ToolsAdd to favoritesDownload citationsTrack citationsPermissions ShareShare onFacebookTwitterLinked InMendeleyRedditDiggEmail Jump toFree AccessResearch ArticlePDF/EPUBReduced Cardiac Myosin Adenosinetriphosphatase Activity in Dogs with Spontaneously Occurring Heart Failure ROBERT J. LUCHI, M.D. EVE MARIE KRITCHER, and A.B. Per T. ThyrumPh.D. ROBERT J. LUCHIROBERT J. LUCHI Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104 Search for more papers by this author , EVE MARIE KRITCHEREVE MARIE KRITCHER Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104 Search for more papers by this author and Per T. Thyrum Per T. Thyrum Department of Medicine, Hospital of the University of Pennsylvania, Philadelphia, Pennsylvania 19104 Search for more papers by this author Originally published1 Apr 1969https://doi.org/10.1161/01.RES.24.4.513Circulation Research. 1969;24:513–519AbstractCardiac myosin was isolated from the hearts of seven dogs with naturally occurring heart failure. Six of the seven dogs had heart failure secondary to acquired mitral valvular insufficiency; the seventh was believed to have a primary myocardiopathy. The characteristics of this myosin were compared to cardiac myosin from a group of normal dogs studied concurrently.Cardiac myosin was extracted from heart muscle with a phosphate salt solution and purified by repeated fractionation with ammonium sulfate in the presence of 2M lithium chloride. Myosin from dogs with heart failure had a significantly reduced adenosinetriphosphatase activity compared to myosin from the control group. Sulfhydryl group content, shown to influence myosin enzyme activity, was unchanged in myosin from dogs with heart failure. Molecular weight of myosin was similar in both groups. Sedimentation velocity of myosin from dogs with heart failure was suggestive of a configurational change in the molecule, but this was not confirmed by measurement of the intrinsic viscosity and helical content of the protein. These studies suggested that the reduced contractile performance of the myocardium in congestive heart failure complicating a disease process is, in part at least, the result of depressed myosin adenosinetriphosphatase activity. Previous Back to top Next FiguresReferencesRelatedDetailsCited ByHorowits R and Winegrad S (1987) cAMP regulation of myosin ATPase activity in the maturing rat heart., Circulation Research, 61:6, (914-924), Online publication date: 1-Dec-1987.Scheuer J and Bhan A (1979) Cardiac contractile proteins. Adenosine triphosphatase activity and physiological function., Circulation Research, 45:1, (1-12), Online publication date: 1-Jul-1979.Carey R, Natarajan G, Bove A, Coulson R and Spann J (1979) Myosin adenosine triphosphatase activity in the volume-overloaded hypertrophied feline right ventricle., Circulation Research, 45:1, (81-87), Online publication date: 1-Jul-1979.Raszkowski R, Welty J and Peterson M (1977) The amino acid composition of actin and myosin and Ca2+-activated myosin adenosine triphosphatase in chronic canine congestive heart failure., Circulation Research, 40:2, (191-198), Online publication date: 1-Feb-1977.Tomlinson C, Lee S and Dhalla N (1976) Abnormalities in heart membranes and myofibrils during bacterial infective cardiomyopathy in the rabbit., Circulation Research, 39:1, (82-92), Online publication date: 1-Jul-1976.HENRY P, AHUMADA G, FRIEDMAN W and SOBEL B (1972) Simultaneously Measured Isometric Tension and ATP Hydrolysis in Glycerinated Fibers from Normal and Hypertrophied Rabbit Heart, Circulation Research, 31:5, (740-749), Online publication date: 1-Nov-1972.ROVETTO M, MURPHY R and LEFER A (1970) Cardiac Impairment in Adrenal Insufficiency in the Cat , Circulation Research, 26:4, (419-428), Online publication date: 1-Apr-1970. April 1, 1969Vol 24, Issue 4Article InformationMetrics Download: 72 © 1969 American Heart Association, Inc.https://doi.org/10.1161/01.RES.24.4.513 Manuscript receivedJuly 10, 1968Originally publishedApril 1, 1969 Keywordsbiochemistry of myosincanine heart myosinadenosinetriphosphatase activitycardiac failurecontractile proteinPDF download