Our previous study on post‐MI male and female middle‐aged rats has revealed the regional sex‐specific differences in the adaptive changes of the left ventricular (LV) cardiac myocytes and coronary vessels. However, the impact of such structural alterations on LV systolic properties remained unclear. Therefore, our current study is aimed to determine whether post‐MI cardiac remodeling in middle‐aged rats associates with sex‐related differences in LV systolic performance, function or contractility. A large MI was induced in 12‐month‐old male (M‐MI) and female (F‐MI) Sprague‐Dawley rats by ligation of the left coronary artery. Two months after the surgery, the various LV parameters were compared between the sexes using echocardiography, hemodynamics and morphometry. We found that although both sexes had a similar scale of global post‐MI LV remodeling, the F‐MI rats showed a significantly lesser degree of cardiac myocyte hypertrophy in the epimyocardium compared to males (353.3±21.4 vs. 479.0±20.6 μm², P蠄0.01). Nevertheless, rats in M‐MI and F‐MI groups demonstrated comparable values of stroke volume (0.23±0.03 vs. 0.17±0.02 mL), ejection fraction (28.1±3.9 vs. 38.68±4.74 %) and peak dP/dt (3068.3±240.6 vs. 3307.0±214.1 mmHg/sec). Thus, our data reveal that male and female middle‐aged rats have a similar degree of deterioration in LV systolic properties associated with cardiac remodeling after a large MI.