Hypercholesterolemic Fatty Acids Research Articles

Comparison of the Fatty Acid Composition and Lipid Nutritional Indices of Meat in Two Lines of Slow-Growing Chickens and Their F1 Crosses

The study was carried out to compare the fatty acid profile and related lipid nutritional indices in the breast and thigh meat of two slow-growing lines of chickens La Belle (LB) and Bresse Gauloise (BB) and their crosses (♂LBx♀BB, ♂BBx♀LB). The birds were reared indoors in the experimental poultry farm of the Institute of Animal Science –Kostinbrod, Bulgaria. At the age of 12 weeks, 6 male chickens of each line were slaughtered and lipid analysis of the breast and thigh meat was done. The differences in the fatty acid profile and related nutritional indices of the meat between the lines were assessed by one-way ANOVA. The fatty acid composition was affected by the crossing of the lines but to a different extent in breast and thigh meat. The highest amount of monounsaturated fatty acids (MUFA) was observed in the meat of ♂BBx♀LB chickens due to the significant increase of C16:1n-7 in breast and C18:1n-9 in the thigh of these birds. On the other hand, this crossbred line exhibited the lowest levels of polyunsaturated fatty acids (PUFA). Furthermore, the lowest content of MUFA was observed in the breast of the pure lines and thigh in BB and ♂LBx♀BB, while the highest PUFA level was determined in the breast of LB and thigh of ♂LBx♀BB. The differences in the meat dietetic quality described by the lipid indices showed no consistent patterns in the pure and crossbred lines depending on the type of meat. When compared with the rest, the breast meat of the LB male chickens showed significantly higher polyunsaturated/saturated fatty acids (P/S) ratio, while lowest n-6/n-3 PUFA and thrombogenic index (TI). The values of the atherogenic index (AI) and the ration between the hypo-and hypercholesterolemic fatty acids (h/H) were also improved in this line. Thigh meat however, showed best characteristics in terms of P/S and n-6/n-3 in the ♂LBx♀BB cross.

Comparative milk metabolite profiling for exploring superiority of indigenous Indian cow milk over exotic and crossbred counterparts.

This study was planned to identify differences in the milk metabolite composition of Indian (Sahiwal), exotic (Holstein-Friesian) and their crossbred cows in intensive system of management. To mimic the management system of ancient India, indigenous cattle under extensive system (zero input) were also included. Holstein-Friesian (HF) had significantly higher amount of saturated fatty acids (SFA, 76.3%) as compared to the crossbred (73.3%) and Sahiwal (68.0%). HF had the highest concentration (42.7%) of hypercholesterolemic fatty acids and the maximum value (68.5) of athrogenecity index (AI). Sahiwal had the highest proportion (32.1%) of total unsaturated fatty acids (UFA). Mineral, vitamin, n-3 fatty acids and total amount of essential amino acids did not vary across the three groups. Milk of indigenous cattle maintained only on grazing had more favorable nutrient profile. It had low SFA (61.4%), high UFA (38.6%) and higher concentrations of both monounsaturated fatty acids (31.4%) and polyunsaturated fatty acids (7.2%). The n-6/n-3 ratio (2.7) and the AI (33.9) were significantly lower. Significantly higher concentrations of minerals (Zn, Fe, P and Cu) and vitamins except vitamin B5 were recorded in their milk. The study revealed that milk metabolite characteristics can be used to promote indigenous cattle.

Effects of soybean silage on feeding behavior, performance, and meat quality of lambs

The objective of this study was to evaluate the performance, feeding behavior, carcass traits, and meat quality of feedlot lambs using 2 types of silage, sorghum or soybean. Twenty-eight male non-castrated market lambs weighting 20 ± 2 kg were used. Diets were formulated to be isonitrogenous and isocaloric. The animals were slaughtered after 50 days of experiment, with 32.13 ± 5.09 kg of body weight. Lambs fed with soybean silage showed increased time for feed intake (P = 0.01). There was no difference for allowed daily gain, dry matter intake, feed conversion, and carcass traits between diets. Meat from lambs fed with soybean silage showed decreased shear force (P = 0.001) compared to sorghum silage, with no differences (P > 0.05) for chemical composition, color, and cooking loss. The intake of sorghum silage increased (P < 0.05) C6:0, C16:0, C20:0, C14:1, and C16:1 fatty acids content of Longissimus lumborum intramuscular fat. The intake of soybean silage showed an increased (P < 0.05) content of C18:1ω9t, C18:2ω6c, and sum of ω6 fatty acids. The feed cost and profit from the use of sorghum and soybean silage were US$ 0.12 and US$ 0.13/Lamb/d, US$ 16.68 and US$ 16.97/Lamb, respectively. The use of soybean silage provides the same performance and carcass traits of lambs compared with sorghum silage, and it shows the benefit of decrease shear force and the proportion of hypercholesterolemic fatty acids C16:0 and C16:1.

Mercury, Fatty Acids Content and Lipid Quality Indexes in Muscles of Freshwater and Marine Fish on the Polish Market. Risk Assessment of Fish Consumption.

Mercury content and fatty acids in muscles of Perca fluviatilis L. (European perch), Leuciscus idus L. (ide), Cyprinus carpio L. (European or common carp), Oncorhynchus mykiss Walb. (rainbow trout), Platichthys flesus L. (European flounder). and Clupea harengus L. (bream) from the Polish market were investigated. The total mercury was processed with AAS. The fatty acids were analyzed by gas chromatography. The concentration of mercury in muscles varied from 0.006 to 0.138 mg/kg and decreased as follows: perch ≈ ide > flounder > herring ≈ bream ≈ rainbow trout > carp (p ≤ 0.05). There were only significant positive correlations between body weight and mercury content in muscle tissue of carp (r = 0.878), flounder (r = 0.925) and herring (r = 0.982) (p ≤ 0.05). The atherogenic index (AI), thrombogenicity index (TI) and flesh-lipid quality index (FLQ) were calculated as follows 0.33–0.70 (IA), 0.16–0.31 (IT) and 13.01–33.22 (FLQ). Hypocholesterolemic (OFA) and hypercholesterolemic fatty acids (DFA) in muscles of fish ranged from 18.26 to 23.01 and from 73.91 to 78.46, respectively. In most cases, there were not significant correlations between size (body weight and total length) and fatty acids in the muscles of the examined fish (p > 0.05). The Target Hazard Quotient (THQ) values were below 1, which shows that there is no non-carcinogenic health risk to the consumer by consuming the examined fish.

Nutritional quality of different fish species farmed in the Adriatic Sea

The nutritional quality of commercially important farmed fish species, i.e. sea bream (Sparus aurata), sea bass (Dicentrarchus labrax), dentex (Dentex dentex) and turbot (Scophthalmus maximus) was investigated. Dentex was shown to have twice as high omega-3 fatty acid content and two to four times higher eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid content as compared to other fish species. The recommended n-6/n-3 ratio was determined in all fish species, whereas the recommended PUFA/SFA ratio was not seen in turbot. Except of turbot, all other fish species matched the recommended atherogenic index (AI), the thrombogenic index (TI) and the ratio of hypocholesterolemic over hypercholesterolemic fatty acids (HH), while the highest the flesh lipid quality (FLQ) value was determined in dentex.

Assessment of nutritional and lipid quality of salted cereal bars prepared with different binding agents

The aim of this paper was to assess the nutritional and lipid quality of salty cereal bars preparedusing different binding agents. The experiment consisted of preparing four cereal formulations with four different binding agents (collagen, guar gum, xanthan gum and psyllium). The physicochemical characteristics and main minerals of the bars were assessed to determine the nutritional value, atherogenic index and thrombogenic index. The ratios between hypocholesterolemic and hypercholesterolemic fatty acids were determined to assess the lipid quality of the product. The results show that the bars have considerable lipid content, carbohydrates, protein, fiber and energetic value. The atherogenicindexindex, thrombogenic index, and ratios between hypocholesterolemic and hypercholesterolemic fatty acids for all the formulations had good lipid quality, which indicates that the bars are a healthy food alternative.

The effect of cattle breed, season and type of diet on the fatty acid profile of raw milk

Abstract. The aim of the study was to determine the effect of cow breed, season and type of diet on the fatty acid (FA) profile of raw milk. A 2-year study was conducted on bulk milk samples collected from eight herds consisting of Czech Fleckvieh (CF, four herds) and Holstein (H, four herds) breeds. One half of the herds of each breed was grazed (G), while the other half was not (N). Samples were collected twice in winter (W) and twice in summer (S). Milk yield in CF (5385.50 kg) was lower than in H (7015.15 kg, P &lt; 0.05). The effect of breed was found in odd-chain, branch-chain and hypercholesterolemic FAs (P &lt; 0.05). The content of fat was lower in summer (S) than in winter (W), being 3.71 and 3.91 g 100 g−1, respectively (P &lt; 0.05). The proportion of saturated and polyunsaturated FAs was lower in S than in W (P &lt; 0.05). The content of monounsaturated FAs was higher in S (30.69 g 100 g−1) than in W (27.72 g 100 g−1, P &lt; 0.05). Milk yield in grazing herds (G, 5197.50 kg) was lower (P &lt; 0.05) than in non-grazing herds (N, 7203.75 kg). The sum of saturated and hypercholesterolemic FAs was lower and the sum of monounsaturated and odd-chain FAs was higher in G than in N (P &lt; 0.05). Content of conjugated linoleic acid (CLA) and C18:3n3 was higher in G (0.93 and 0.64 g 100 g−1) than in N (0.42 and 0.39 g 100 g−1, respectively, P &lt; 0.001).

Fatty acid profile and health lipid indices in the raw milk of ewes grazing part-time and hemp seed supplementation of lactating ewes

This experiment was designed to evaluate the effects of the feeding system (indoor or part-time grazing) of ewes and dietary supplementation with hemp seed (non-drug varieties of Cannabis sativa L.) on milk production and the composition of milk fatty acids (FAs). The experimental diets were constituted as a 2 x 2 factorial arrangement of feeding system (I: indoors and receiving 1400 g dry matter (DM) grass hay/d or pasture grazing (PG)–4 h/day + 700 g DM grass hay/d), and with or without hemp seed (Hs) supplementation (-Hs = no hemp seed vs. +Hs = 175 g/d hemp seed). All animals received 700 g DM/day of a concentrate for lactation. Feeding the PG diets increased raw milk production and yields of fat, protein and lactose. Feeding +Hs increased fat-corrected milk, milk fat content and fat yield significantly while the combination of +Hs and PG increased fat-corrected milk and fat yield significantly. Milk protein content was not affected by dietary factors. Feeding the I diets increased the proportion of short-chain FAs (C4:0 - C10:0), at the expense of medium-chain FAs (12 to 17 C units). Feeding +Hs decreased the proportion of short-chain FAs, medium-chain FAs and hypercholesterolaemic FA (sum of C12:0, C14:0, C16:0) and increased the trans-vaccenic acid (C18:1 t11, VA), α-linolenic acid (C18:3 n-3, ALA), conjugated linoleic acid (CLA), eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) concentrations in milk fat significantly. The combination of hemp seed and part-time grazing produced the highest proportion of c9,t11 CLA, t10,c12 CLA and n-3 FA (sum of ALA, EPA, DHA) in milk fat. The polyunsaturated fatty acid (PUFA)/saturated fatty acid (SFA) ratios in the milk from ewes grazing part time were significantly higher, whereas the atherogenicity index (AI), thrombogenic index (TI) and linoleic acid (LA)/ALA ratio were significantly lower compared with the milk of indoor-fed ewes. Supplementing with +Hs has a similar effect of increasing the PUFA/SFA ratio and lowering of the AI, TI and LA/ALA ratio. It was concluded that pasture grazing combined with hemp seed had a synergistic effect on milk production and fat yield without altering the milk protein content in ewes. This combination has favourable effects on milk FA profile from the human consumer’s point of view by increasing the nutritional quality of milk.Keywords: α-linolenic acid, atherogenicitiy index, Cannabis sativa, c9,t11 CLA, n-6/n-3 FA

Difference in fatty acid composition and related nutritional indices of meat between two lines of slow-growing chickens slaughtered at different ages

Abstract. The fatty acid profile and the related indices of the nutritional quality of breast and thigh muscles were studied in two lines of chickens – La Belle (LB) and White Plymouth Rock (WPR) – slaughtered at the age of 9 and 18 weeks. The fatty acid profile was more affected by the age than the line of the birds; however, the influence of both differed between the breast and thigh. The content of total saturated fatty acids (SFAs) increased in the thigh (P &lt; 0.01), while that of the monounsaturated fatty acids (MUFAs) decreased with age in both muscles (P &lt; 0.001). This corresponded to the significant decrease in C18:1 in the older chickens and the lower desaturase activity (P &lt; 0.001). The contents of C20:4n-6, C22:5n-3 and C22:6n-3 and the total amount of polyunsaturated fatty acids (PUFAs) in breast were higher (P &lt; 0.001) at the age of 18 weeks. A similar pattern in the individual and total PUFA was observed in the thighs. The effect of line was more visible in the breast, leading to a lower C14:0 content and C20:5n-3 and a higher C18:0 content in the WPR chickens (P &lt; 0.001), corresponding to the higher elongase and thioesterase indices in these birds. Both atherogenic (AI) and thrombogenic (TI) indices were lowered, while the ratio of hypocholesterolemic ∕ hypercholesterolemic fatty acids (h ∕ H) and polyunsaturated ∕ saturated fatty acids (P ∕ S) increased in the breast of the birds at 18 weeks. In breast and thigh meat, the ratio of n-6 ∕ n-3 PUFA decreased in the older chickens (P &lt; 0.001).

Fatty acid profile, total cholesterol, vitamin content, and TBARS value of turkey breast muscle cured with the addition of lycopene

The aim of this study was to determine the effect of lycopene addition for curing turkey meat on the profile of fatty acids, total cholesterol, vitamin content, and the TBARS of the final products. The analyzed material comprised 64 breast muscles, of which 16 (RBM) were immediately transported to a laboratory. Another 16 (UBM) were heat treated in a convection steam oven, and 32 muscles were cured for 3 days in two types of curing mixture: without (CBM) and with (CBM+Lyc) tomato peel extract standardized for 5% lycopene content. After completed curing, samples were steamed and grilled under the same conditions as raw samples. Statistical analysis demonstrated the highest (P ≤ 0.01) mean content of vitamin A (0.07 μg/g) in chilled muscles. The content of vitamin E was lower (P ≤ 0.01) in UBM samples than in CBM+Lyc and RBM. The TBARS value was the lowest (P ≤ 0.01) in RBM muscles (0.35 mg MDA/kg of meat). Although there were no differences between products, but lower TBARS were found in CBM+Lyc samples. The content of cholesterol was higher (P ≤ 0.01) in CBM+Lyc products than in the RBM and UBM. RBM samples contained (P ≤ 0.01) the lowest amount of saturated, monounsaturated, and hypercholesterolemic fatty acids, and the highest of unsaturated, polyunsaturated, and hypocholesterolemic fatty acids. CBM+Lyc samples contained (P ≤ 0.01) less hypercholesterolemic and more hypocholesterolemic fatty acids than CBM group. Higher (P ≤ 0.01) unsaturated/saturated and hypocholesterolemic/hypercholesterolemic fatty acid ratios were also found in CBM+Lyc products. The study demonstrated that the used processing technology caused reduction (P ≤ 0.01) of n-3 and n-6 PUFA content. Findings suggest that the addition of lycopene in the process of meat curing and heat treatment in meat industry do not change the content of vitamins and cholesterol or alter the TBARS value in turkey meat products. Nevertheless, lycopene can be used to increase the content of essential hypocholesterolemic fatty acids and decrease the content of saturated hypercholesterolemic fatty acids.

Seasonal Composition of Lipids, Fatty Acids Pigments in the Brown Alga &amp;lt;i&amp;gt;Sargassum pallidum&amp;lt;/i&amp;gt;: The Potential for Health

The present study describes the content of total lipids (TL), the main components of polar and neutral lipids (NL) and photosynthetic pigments (PSP) in edible seaweed Sargassum pallidum (Ochrophyta) from the Sea of Japan, Russia, in different months of the year, taking into account life cycle. Special attention was paid to the fatty acids (FAs) of total lipids as well as the accumulation of important polyunsaturated fatty acids (PUFAs) in glyceroglycolipids (GL) and reserve lipids in the seasonal cycles of growth. The content of TL strongly varied in the course of a year. The major lipid components were GL (20.3% - 36.4%) and NL (19.8% - 30.6%), while phospholipids (PL) were present in negligible amounts (3.2% - 6.9%). Significant seasonal variations were observed in content all classes of lipids. The PUFAs prevailed in total lipids and the content of n-6 PUFAs was higher than n-3 PUFAs on all stages of life with the exception of vegetation period. Monogalactosyldiacylglycerols (MGDG) and digalactosyldiacylglecerols (DGDG) were rich in PUFAs and n-3 PUFAs were dominated among them independently of stages of growth. Saturated fatty acids (SFA) were dominated in sulfoquinovosyldiacylglycerols (SQDG). Triacylglycerols (TAG) and diacylglycerols (DAG) contained many PUFAs, especially n-6 PUFAs. Significant concentration 20:3 n-6 was in the triacylglycerols. The concentration of chlorophylls and carotenoids was increased with the growth and maturation of S. pallidum and reached maximum in period of highest day length and water temperature. The content of pigments decreased with the onset of short days and decreasing water temperatures. Nutritional value and benefit for health of human this alga were evaluated. The n-6/n-3 PUFAs ratio, nutritional quality index (atherogenic and thrombogenic indices, and ratio between hypocholesterolemic and hypercholesterolemic fatty acids) were low, suggesting a high nutritional value of S. pallidum throughout the year.

Assessment of lactation stage and breed effect on sheep milk fatty acid profile and lipid quality indices

Information on fatty acid (FA) profile is critical for the production and promotion of sheep milk and derivative dairy products. The presence of the essential ω-3 and ω-6 FA in milk fat as well as other less common FA, like linoleic acid isomers, has gained an increasing interest due to the consumer demand for a healthy diet. This research assesses the FA profile and estimates the lipid quality indices (ratio between hypocholesterolaemic and hypercholesterolaemic fatty acids, peroxidisability index, atherogenic index, and thrombogenic index) of raw milk and cream fat from two indigenous Greek sheep breeds (Karagouniko and Chios) at different lactation stages. Raw milk and cream fat presented a favorable ω-6/ω-3 ratio below 4:1. Atherogenic and thrombogenic indices of all studied milk fat fluctuated in sufficiently low levels (<3). The FA profile and lipid quality indices in both raw milk and cream samples differed significantly depending more on the lactation stage compared to the breed type. Raw milk fat from late lactation had more beneficial fatty acid profile compared to early and middle lactation stages. Differences among breeds were highlighted when raw milk and cream samples were compared within the same lactation stage. Raw milk and cream fat from Karagouniko breed were characterised by higher ω-3 proportion, lower ω-6/ω-3 ratio and lower thrombogenic index value compared to those from Chios breed.

Dietary effect of silage type and combination with camelina seed on milk fatty acid profile and antioxidant capacity of sheep milk

The present study sought to quantify the differences between maize-based (MS) and grass-silage-based (GS) diets in terms of their effect on the milk yield, milk fatty acid composition and antioxidant capacity in dairy ewes, and to test the hypothesis that it is possible to improve yield, fatty acid (FA) composition and antioxidant capacity by supplementing diet with camelina seed (Cs). Experimental diets consisted of a 2 x 2 factorial arrangement of type of silage (GS vs. MS) and camelina seed (−Cs vs. +Cs). Feeding the MS diets increased net energy for lactation (NEL) intake, raw milk yield and fat, protein and lactose yields. Feeding +Cs increased energy corrected milk (ECM), milk fat content and fat yield. Maize silage consumption is associated with an increased proportion of hypercholesterolemic fatty acids (HFA) and a higher value of the atherogenicity index. However, an MS diet led to an increased share of t11-C18:1 and c9,t11-conjugated linoleic acid (CLA) in milk. Milk FA profile in ewes fed GS diet was of higher quality for human beings owing to higher concentrations of α-linolenic acid and a lower content of HFA. Supplementing with camelina seed resulted in a higher concentration of t11-C18:1, c9,t11-CLA and C18:3n-3 in milk fat. The trolox equivalent antioxidant capacity (TEAC) value of milk was higher in milk from MS-fed ewes compared with that of their counterparts fed GS. Dietary supplementation with camelina seed increased the oxidative stability of milk samples. These results suggest that grass-silage-based diet supplemented with camelina seed results in milk of better quality for human consumption.Keywords: Milk quality, oilseed, PUFA profile, oxidative stability of milk, TEAC assay

Desempenho produtivo e perfil de ácidos graxos do leite de vacas que receberam níveis crescentes de óleo de girassol em dietas à base de capim-elefante

Este experimento teve como objetivo principal avaliar as alterações no perfil de ácidos graxos do leite decorrentes do fornecimento de diferentes níveis de óleo de girassol (OG) para vacas leiteiras alimentadas com dietas completas à base de capim-elefante. Doze vacas da raça Holandesa receberam quatro níveis de OG na dieta (0; 1,3; 2,5 e 3,7% da MS) em delineamento quadrado latino 4 x 4. Não houve efeito (P&gt;0,05) dos níveis de OG sobre o consumo de MS, a produção de leite ou os teores de sólidos do leite. Por outro lado, o perfil de ácidos graxos (AG) do leite foi amplamante modificado com a inclusão de OG na dieta, e observou-se redução (P&lt;0,0001) dos teores dos AG de cadeia curta e média, elevação dos teores de ácido oleico (P&lt;0,0001), dos isômeros de ácido linoleico conjugado, em especial do ácido rumênico (P&lt;0,0001) e da relação entre os ácidos graxos hipo e hipercolesterolêmicos (P&lt;0,0001). Apesar da maior ingestão diária de ácido linoleico (P&lt;0,01) com o aumento do nível de OG na dieta, sua concentração na gordura do leite não foi alterada. Isso indica extensiva bio-hidrogenação ruminal deste AG no rúmen, o que é compatível com o aumento (P&lt;0,0001) da concentração de ácido esteárico e dos AG C18:1 trans, especialmente do vacênico, na gordura do leite. Em geral, os resultados observados no presente estudo indicam que a inclusão de até 3,7% de OG em dietas completas de vacas leiteiras à base de capim-elefante promove melhoria da qualidade nutricional da gordura do leite, sem comprometimento do desempenho produtivo dos animais.

Effect of cow energy status on the hypercholesterolaemic fatty acid proportion in raw milk

We evaluated the proportion of fatty acid groups, with an emphasis on hypercholesterolaemic fatty acids, in the milk of 25 Holstein cows during the 1&lt;sup&gt;st&lt;/sup&gt; period of lactation in relation to their negative energy balance (NEB). Sampling of each cow&amp;rsquo;s milk started on the 7&lt;sup&gt;th&lt;/sup&gt; day after calving. Milk samples (n = 425) were collected at 7-day periods during the first 17 weeks of lactation. The proportion (%) of saturated (SFA), hypercholesterolaemic (HCFA), volatile (VFA), unsaturated (UFA), monounsaturated (MUFA), and polyunsaturated (PUFA) fatty acids in the milk fat was determined. Body condition score and fat to protein ratio in milk were applied for precise determination of the NEB breakpoint during the observed period. The effects of parity, NEB, regression on lactation week and fat to protein ratio were evaluated using SAS 9.3. Milk contained a lower proportion of SFA as well as equally higher UFA (&amp;plusmn; 2.13%; P &amp;lt; 0.01) during the NEB period. The overcoming of NEB caused an increase in SFA, however, and simultaneously a significant decline in total HCFA (&amp;ndash;1.86%; P &amp;lt; 0.01) as well as main MUFA (&amp;ndash;1.81%, P &amp;lt; 0.05). The results document the necessity of increasing Holstein cow robustness to meet the production conditions in dairy farms in relation to the requirement of higher nutrient quality as well as the potential health benefits of cow&amp;rsquo;s raw milk for consumers.

Fatty acid composition in hybrid pigs as based on local fatty Lithuanian breed and wild boar

The study was designed to investigate the fatty acid composition in longissimus dorsi (LD) and semimembranosus muscles (SM) and subcutaneous tissue of hybrid (1/4 Lithuanian indigenous wattle, 1/4 wild boar and 1/2 Yorkshire or 1/2 Landrace) pigs. The SM showed a tendency to have lower contents of monounsaturated fatty acids (MUFA) and higher contents of polyunsaturated fatty acids (PUFA) than the LD muscle. No statistically significant differences were observed between the muscles in lipid quality indices, i.e., atherogenic (AI) and thrombogenix (TI) indices and the ratio of hypocholesterolemic and hypercholesterolemic fatty acids (h/H). In the SM, the content of PUFA from Yorkshire-sired hybrids was relatively 47.8% higher, including C18:2n-6, C20:4n-6 and DPA (C22:5n-3) that were higher, respectively, by 44.3%, 86.6% and 51.6% in comparison with those from Landrace-sired hybrids. The gilts had a lower content of MUFA, including C18:1 and C20:1n-9 fatty acids in the LD muscle and tended to have a higher content of PUFA in the SM compared to the barrows. Neither the terminal breed in crossing, nor the gender had any significant effects on the proportions of total saturated, MUFA and PUFA in the subcutaneous tissue.

Perfil de ácidos graxos no Longissimus dorsi de cordeiros Santa Inês alimentados com diferentes níveis energéticos

This study evaluated the influence of rations with different levels of metabolizable energy on the content of total lipids, cholesterol and fatty acid profile of the longissimus dorsi of Santa Ines lambs. Twenty Santa Ines lambs were used, with age and body weight of 50 days and 13.0 kg ± 0.56 kg, respectively, fed rations with different energy levels: 2.08; 2.28; 2.47 e 2.69 Mcal/kgDM. Animals were weighed weekly to follow their body weight gain (BWG); when the mean BW of the treatment reached 28 kg, the animals were slaughtered. The analysis of the lipid profile of Longissimus dorsi presented linear increasing and decreasing for the Cis-10 heptadecanoic acid (C17:1) and eicosatrienoic (C20:3), respectively, with the increasing dietary energy levels (P &lt; 0.05). Cholesterol levels (mg/100g) decreased in relation to the increase in energy levels in the diet. The relations polyunsaturated fatty acids (PUFA): saturated fatty acids (SFA); PUFA: monounsaturated fatty acids (MUA), MUA: SFA, desirable fatty acids, w-6:w-3, atherogenicity index, thrombogenicity index, relation between hypocholesterolemic and hypercholesterolemic fatty acids and the relation (C18:0+C18:1):C16:0 were not affected by energy levels (P&gt;0.05). The dietary manipulation influences the lipid profile in Longissimus dorsi of Santa Ines lambs.

Interaction between soybean oil and the lipid fraction of fried pitu prawn

The interaction between the soybean oil used to fry pitu prawn and the lipid fraction was assessed from the changes on its composition of fatty acids, cholesterol content and the formation of cholesterol oxides. The proximate composition, caloric value and nutritional quality were determined. Frying in soybean oil increased the lipid, calories and n-6 polyunsaturated fatty acids (n-6 PUFA) contents and reduced the n-3 PUFA, consequently increasing n-6/n-3 ratio. Cholesterol and its oxidation products remained unchanged after frying. The ratio of hypocholesterolemic:hypercholesterolemic fatty acids (HH) observed in raw and fried samples was high and the values of the atherogenicity (AI) and thrombogenic indexes (TI) were low and decreased after frying. The fried pitu prawn can be considered a good source of protein, monounsaturated fatty acids (MUFA), n-6 PUFA, n-3 PUFA, especially eicosapentaenoic acid (EPA), and low in cholesterol and cholesterol oxides.

Fatty acids distribution in different tissues of wild and reared Seriola dumerili

In this study, we compared the lipid and fatty acids content, between cultured and wild Seriola dumerili, in different edible portions. Results showed that cultured fish contained a higher level of lipids than wild fish. The fatty acids profiles revealed that, among all the split-fish side, palmitic (C16:0) and oleic (C18:1n-9) acid were the principal saturated and monounsaturated fatty acids, in cultured and wild S. dumerili. On the other hand, wild S. dumerili contained a higher level of saturated (38.12 ± 0.54% vs. 33.66 ± 0.15% in cultured fish), monounsaturated (33.13 ± 1.07% vs. 26.49 ± 0.17%), n-3 polyunsaturated fatty acids (PUFA) (23.90 ± 1.02% vs. 19.77 ± 0.51%) particularly docosahexaenoic acid (DHA) (18.83 ± 0.48% vs. 11.77 ± 0.42%). However, the cultured fish showed a higher level of n-6 PUFA due principally to the higher value of linoleic acid (C18:2n-6). In fact, changes in fatty acids content between anatomical areas marked differences in the muscle quality of wild and cultured S. dumerili. According to this study, both groups of wild and farmed S. dumerili have nutritional benefits for human health. Cultured fish were characterised by higher hypocholesterolaemic and hypercholesterolaemic fatty acids ratio for all samples studied and a lower n-3/n-6 ratio due to the abundance of n-3 PUFA particularly DHA in wild fish.

Variation in Milk Fatty Acid Composition with Body Condition in Dairy Buffaloes (Bubalus bubalis)

Buffaloes usually maintain higher body condition and do not produce milk at the cost of their own body reserves under tropical conditions. The mobilization of body reserves for fulfilling the demands of lactation has been extensively studied in dairy cows while limited work is available on this aspect in dairy buffaloes. Therefore, the present study was conducted to examine variations in milk fatty acid profiles with body condition in Nili-Ravi buffaloes. A total of 24 Nili-Ravi buffaloes within 60 days after parturition, were selected from a private dairy farm in the district of Peshawar. All animals consumed the same diet during the experimental period. A total of 576 raw milk samples were collected for laboratory analysis. The study continued up to 6 months during 2008. Body condition score (BCS), milk yield and composition were recorded once a week. Means for milk fatty acid profile were compared for various levels of BCS. The mean milk yield and fat content were 9.28 kg/d and 5.36%, respectively. The total saturated fatty acids (SFA) were 64.22 g/100 g and the unsaturated fatty acids (UFA) were 35.79 g/100 g. Of the SFA the highest amount was recorded for , followed by , and . The total sum of hypercholesterolemic fatty acids (HCFA, , and ) was 43.33 g/100 g. The concentrations of UFA were greater for moderate BCS followed by poor and highest BCS while SFA showed the opposite trend. The correlation analysis showed that milk yield was negatively affected by BCS and milk fat positively affected, though non-significantly. The present study suggests that Nili-Ravi dairy buffaloes produce similar milk to dairy cows regarding availability of cardioprotective fatty acids, with the highest concentration of . Two HCFA ( and ) were associated with higher body condition. Buffaloes with moderate body condition yielded milk containing healthier fatty acids.