Ventilation must constantly change to meet varying metabolic and acid‐base regulation needs of the organism. Although O2 and CO2/pH sensors have received much attention for the chemical control of breathing, metabolites such as lactate and ATP have recently been shown to play important roles in both the hypoxic and hypercapnic ventilatory responses of vertebrates. In mammals and fish, lactate anions increase ventilation through peripheral mechanisms, but the actions of lactate in the central respiratory network of any vertebrate species remain unclear. Anuran amphibians (frogs and toads) accumulate large amounts of lactate in the brain during periods of high respiratory drive, suggesting that lactate may act at central sites to stimulate breathing. For example, hypoxia increases brain lactate which may contribute to elevated ventilation following physiological hypoxia caused by breath‐holding. Furthermore, anurans perform mainly anaerobic exercise, leading to large lactate loads in the blood that may get delivered to the brain through transporters present in the blood‐brain barrier. Thus, we tested the hypothesis that lactate stimulates output from the central respiratory network in amphibians. To test this hypothesis, we measured respiratory‐related cranial nerve X (CNX) and hypoglossal nerve (SNII) activity in isolated brainstem‐spinal cord preparations from the American bullfrog, Lithobates catesbeianus, in response to sodium lactate (lac−; n=8). The brainstem‐spinal cord preparation is advantageous because we can assess the actions of lac− on rhythm generating and motor functions of the respiratory network independent of peripheral feedback. Lac− failed to increase frequency of the respiratory rhythm and amplitude of CNX output. However, we identified a striking dose‐dependent increase in amplitude of respiratory‐related SNII output in each preparation, indicating enhanced motor neuron firing and/or recruitment by lac− (P=0.008; Friedman test; 0.5 mM= 33±11, 2 mM= 56±19, 4 mM= 89±29 % increase above baseline). To control for slight increases in [Na+], osmolality, extracellular pH, and time during exposure to sodium lactate, we superfused a separate group of preparations with 0.5, 2, and 4 mM sodium bicarbonate (HCO3−; n=4). HCO3− did not increase the motor amplitude of either SNII or CNX (P>0.9; Friedman test). Thus, lactate anions lead to increased respiratory motor output of SNII, which may enhance activation of buccal floor musculature during periods of high respiratory demand such as hypoxia and exercise. Overall, this work directly implicates lactate anions in the control of central motor networks that regulate breathing in vertebrates.Support or Funding InformationThis work was funded by start‐up funds provided by UNC‐Greensboro to JS.