Aim: The aim of this study was to compare the cyclic fatigue resistance of different heat-treated nickel-titanium rotary systems at intracanal temperature. 
 Methodology: A total of 90 OneCurve (Micro-Mega, Besançon, France), VDW.ROTATE (VDW Dental, Munich, Germany), Typhoon (Clinician’s Choice, New Milford, CT, USA), HyFlex EDM (Coltene/Whaledent AG, Altstatten, Switzerland), and EndoArt Gold and Blue (Inci Dental, Istanbul, Turkey) (n = 15) rotary files (#25/0.06) were tested at intracanal temperature (35.5 ℃) using a dynamic model in a stainless-steel artificial canal with an inner diameter of 1.5 mm, 60° angle of curvature, and 2 mm radius of curvature. Testing was conducted until fracturing, at which time the device stopped automatically, and the number of rotations was calculated as seconds. Lengths of fractured parts were measured using a digital caliper. One-way ANOVA test followed by Tukey’s test was used to compare the groups. Scanning electron microscopic evaluation was performed to confirm the types of fracture.
 Results: EndoArt Blue group had a significantly higher mean time to fracture in all groups, followed by the HyFlex EDM, VDW.ROTATE, OneCurve, EndoArt Gold, and Typhoon. In addition, the HyFlex EDM and VDW.ROTATE groups had no significant differences between each other and were significantly better than the others. No significant differences were found between the OneCurve, EndoArt Gold, and Typhoon groups (p>0.05).
 Conclusion: This is the first study in the literature for EndoArt NiTi files and the second study for VDW.ROTATE that evaluated cyclic fatigue resistance. Novel EndoArt Blue files exhibited significantly greater cyclic fatigue resistance than the other NiTi files.
 
 How to cite this article: Güneç HG, Keskin NB, Haznedaroğlu F. Comparison of cyclic fatigue resistance of different and novel heat-treated nickel-titanium rotary file systems at intracanal temperature. Int Dent Res 2021;11(3):158-64. https://doi.org/10.5577/intdentres.2021.vol11.no3.4
 
 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.