Antifouling materials are highly demanded in global marine market due to increasingly aggravated marine biofouling problem and unbalanced marine ecology. Herein, poly (vinyl imidazole-co-hydroxypropyl methacrylate-co-butyl acrylate) (P(VIm-co-HPMA-co-BA)) was prepared via free radical polymerization of commercially available monomers of vinyl imidazole (VIm), hydroxypropyl methacrylate (HPMA) and butyl acrylate (BA). Then the zwitterionic terpolymer (P(VPS-co-HPMA-co-BA)) was obtained by ionicalization of P(VIm-co-HPMA-co-BA) with 1, 3-propionate lactone (1, 3-PS) in EtOH solutions. Furthermore, the membrane samples, P(VIm-co-HPMA-co-BA) and P(VPS-co-HPMA-co-BA) were prepared by tape casting method with DI water bath. Significantly, P(VPS-co-HPMA-co-BA) membranes have shown better bactericidal activity against Gram-negative Escherichia coli (E. coli) and Gram-positive Staphylococcus aureus (S. aureus) with a mean area size of 3.5 cm2. Meanwhile, the bacterial adhesion assay showed that P(VPS-co-HPMA-co-BA) membranes were found the absence of adherent bacteria, and exhibited excellent anti-adhesion property with the increase of molar ratios of PVPS by the observation of bacteria population density. The mechanical testing results indicated that the molar ratios of 65/10/25 in P(VIm-co-HPMA-co-BA) membranes and P(VPS-co-HPMA-co-BA) membranes had high breaking elongation ratio and great tensile strength. Specifically, the maximum tensile strength of P(VPS-co-HPMA-co-BA) membrane could reach up to 10.9 MPa. The results showed that the novel P(VPS-co-HPMA-co-BA) membrane exhibited both anti-bacterial, anti-adhesion property and mechanical performance, manifesting its prospectively potential applications in new highly efficient and eco-friendly marine antifouling coatings.