Hydrothermal Vent Field Research Articles

Acoustic Predictors of Active Fluid Expulsion From a Hydrothermal Vent Field, Offshore Taupō Volcanic Zone, New Zealand

Understanding fluid expulsion is key to estimating gas exchange volumes between the seafloor, ocean, and atmosphere; for locating key ecosystems; and geohazard modelling. Locating active seafloor fluid expulsion typically requires acoustic backscatter data. Areas of very-high seafloor backscatter, or “hardgrounds,” are often used as first-pass indicators of potential fluid expulsion. However, varying and inconsistent spatial relationships between active fluid expulsion and hardgrounds means a direct link remains unclear. Here, we investigate the links between water-column acoustic flares to seafloor backscatter and bathymetric metrics generated from two calibrated multibeam echosounders. Our site, the Calypso hydrothermal vent field (HVF) in the Bay of Plenty, Aotearoa/New Zealand, has an extensive catalogue of vents and seeps in <250 m water depth. We demonstrate a method to quantitatively link active fluid expulsion (flares) with seafloor characteristics. This allows us to develop predictive spatial models of active fluid expulsion. We explore whether data from a low (30 kHz), high (200 kHz), or combined frequency model increases predictive accuracy of expulsion locations. This research investigates the role of hardgrounds or surrounding sediment cover on the accuracy of predictive models. Our models link active fluid expulsion to specific seafloor characteristics. A combined model using both the 30 and 200 kHz mosaics produced the best results (predictive accuracy: 0.75; Kappa: 0.65). This model performed better than the same model using individual frequency mosaics as input. Model results reveal active fluid expulsion is not typically associated with the extensive, embedded hardgrounds of the Calypso HVF, with minimal fluid expulsion. Unconsolidated sediment around the perimeter of and between hardgrounds were more active fluid expulsion sites. Fluids exploit permeable pathways up to the seafloor, modifying and refashioning the seafloor. Once a conduit self-seals, fluid will migrate to a more permeable pathway, thus reducing a one-to-one link between activity and hardgrounds. Being able to remotely predict active and inactive regions of fluid expulsion will prove a useful tool in rapidly identifying seeps in legacy datasets, as well as textural metrics that will aid in locating nascent, senescent, or extinct seeps when a survey is underway.

Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments.

Viruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3′-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (≥70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean.

Metagenomic and metatranscriptomic analyses reveal minor-yet-crucial roles of gut microbiome in deep-sea hydrothermal vent snail

BackgroundMarine animals often exhibit complex symbiotic relationship with gut microbes to attain better use of the available resources. Many animals endemic to deep-sea chemosynthetic ecosystems host chemoautotrophic bacteria endocellularly, and they are thought to rely entirely on these symbionts for energy and nutrition. Numerous investigations have been conducted on the interdependence between these animal hosts and their chemoautotrophic symbionts. The provannid snail Alviniconcha marisindica from the Indian Ocean hydrothermal vent fields hosts a Campylobacterial endosymbiont in its gill. Unlike many other chemosymbiotic animals, the gut of A. marisindica is reduced but remains functional; yet the contribution of gut microbiomes and their interactions with the host remain poorly characterised.ResultsMetagenomic and metatranscriptomic analyses showed that the gut microbiome of A. marisindica plays key nutritional and metabolic roles. The composition and relative abundance of gut microbiota of A. marisindica were different from those of snails that do not depend on endosymbiosis. The relative abundance of microbial taxa was similar amongst three individuals of A. marisindica with significant inter-taxa correlations. These correlations suggest the potential for interactions between taxa that may influence community assembly and stability. Functional profiles of the gut microbiome revealed thousands of additional genes that assist in the use of vent-supplied inorganic compounds (autotrophic energy source), digest host-ingested organics (carbon source), and recycle the metabolic waste of the host. In addition, members of five taxonomic classes have the potential to form slime capsules to protect themselves from the host immune system, thereby contributing to homeostasis. Gut microbial ecology and its interplay with the host thus contribute to the nutritional and metabolic demands of A. marisindica.ConclusionsThe findings advance the understanding of how deep-sea chemosymbiotic animals use available resources through contributions from gut microbiota. Gut microbiota may be critical in the survival of invertebrate hosts with autotrophic endosymbionts in extreme environments.

Shallow Hydrothermal Vent Bacteria and Their Secondary Metabolites with a Particular Focus on Bacillus.

Extreme environments are hostile for most organisms, but such habitats represent suitable settings to be inhabited by specialized microorganisms. A marine shallow-water hydrothermal vent field is located offshore in northeast Taiwan, near the shallow shore of the southeast of Kueishantao Island (121°55′ E, 24°50′ N). Research on extremophilic microorganisms makes use of the biotechnological potential associated with such microorganisms and their cellular products. With the notion that extremophiles are capable of surviving in extreme environments, it is assumed that their metabolites are adapted to function optimally under such conditions. As extremophiles, they need specific culture conditions, and only a fraction of species from the original samples are recovered in culture. We used different non-selective and selective media to isolate bacterial species associated with the hydrothermal vent crab Xenograpsus testudinatus and the sediments of its habitat. The highest number of colonies was obtained from Zobell marine agar plates with an overall number of 29 genetically distinct isolates. 16S RNA gene sequencing using the Sanger sequencing method revealed that most of the bacterial species belonged to the phylum Firmicutes and the class Bacilli. The present study indicates that hydrothermal vent bacteria and their secondary metabolites may play an important role for the reconstruction of the evolutionary history of the phylum Procaryota.

Unusual aliphatic hydrocarbon profiles at hydrothermal vent fields of the Central and Southeast Indian Ridges and Mid-Indian Basin

The hydrocarbon (n-alkane and n-alkene) distribution in deep seawaters of the Mid-Indian Basin (MIB) and hydrothermal vent sites of the Central (CIR) and Southeast Indian Ridges (SEIR) were investigated to understand the impact of hydrothermal activities in deep seawater organic matter. The distinguishing feature of the detected hydrocarbons was in a bimodal distribution with a strong predominance of even-numbered n-alkenes (C16:1-C34:1) maximizing at n-C16 or n-C18, and a series of n-alkane hydrocarbons ranging from C16–C35 with slightly high content in long chain par but without an even/odd carbon number preference. The mono-unsaturated aliphatics were generally 1–4 times higher than the saturated counterpart and exhibited more complex vertical variabilities. The higher content of aliphatics in deeper water, i.e., the signals at 2500 m from the CIR and the increasing tendency towards the bottom from the SEIR in the near vent field sites, indicate a potential hydrothermal contribution. Sulfate-reducing microorganisms of bacteria and archaeon in seawater and the hydrothermal system may be responsible for the distribution feature of the saturated or unsaturated hydrocarbons, but the possibility of abiogenic sources cannot be excluded. In addition, the hydrocarbon profiles recorded the organic debris mineralization in the subsurface coinciding with dramatically hydrographic variability. The present aliphatic profiles feature reflected the complex bio-geochemical process, a minor impact of hydrothermal activity on the hydrocarbon distributions and deep water circulations in the Indian Ocean.

Soluble, Colloidal, and Particulate Iron Across the Hydrothermal Vent Mixing Zones in Broken Spur and Rainbow, Mid-Atlantic Ridge.

The slow-spreading Mid-Atlantic Ridge (MAR) forms geological heterogeneity throughout the ridge system by deep crustal faults and their resultant tectonic valleys, which results in the existence of different types of hydrothermal vent fields. Therefore, investigating MAR hydrothermal systems opens a gate to understanding the concentration ranges of ecosystem-limiting metals emanating from compositionally distinct fluids for both near-field chemosynthetic ecosystems and far-field transport into the ocean interiors. Here, we present novel data regarding onboard measured, size-fractionated soluble, colloidal, and particulate iron concentrations from the 2018 R/V L’Atalante – ROV Victor research expedition, during which samples were taken from the mixing zone of black smokers using a ROV-assisted plume sampling. Iron size fractionation (<20, 20–200, and >200nm) data were obtained from onboard sequential filtering, followed by measurement via ferrozine assay and spectrophotometric detection at 562nm. Our results showed the persistent presence of a nanoparticulate/colloidal phase (retained within 20–200nm filtrates) even in high-temperature samples. A significant fraction of this phase was retrievable only under treatment with HNO3 – a strong acid known to attack and dissolve pyrite nanocrystals. Upon mixing with colder bottom waters and removal of iron in the higher parts of the buoyant plume, the larger size fractions became dominant as the total iron levels decreased, but it was still possible to detect significant (micromolar) levels of nanoparticulate Fe even in samples collected 5m above the orifice in the rising plume. The coolest sample (<10°C) still contained more than 1μM of only nitric acid-leachable nanoparticle/colloidal, at least 200 times higher than a typical Fe concentration in the non-buoyant plume. Our results support previous reports of dissolved Fe in MAR vent plumes, and we propose that this recalcitrant Fe pool – surviving immediate precipitation – contributes to maintaining high hydrothermal iron fluxes to the deep ocean.

Microbial Communities of the Hydrothermal Scaly-Foot Snails From Kairei and Longqi Vent Fields

The microbial communities of the hydrothermal Scaly-foot Snails (SFSs) from independent hydrothermal vent fields have not been investigated in depth. In this study, we collected SFSs from two different hydrothermal environments located on the Central Indian Ridge (CIR) and the Southwest Indian Ridge (SWIR), the Kairei and Longqi vent fields, respectively. Additionally, one SFS collected from the Kairei vent field was reared for 16 days with in situ deep-sea seawater. The epibiotic and internal samples of SFSs, including ctenidium, esophageal gland, visceral mass, shells, and scales, were examined for microbial community compositions based on the 16S rRNA gene. Our results revealed significant differences in microbial community composition between SFSs samples collected from Kairei and Longqi vent fields. Moreover, the microbial communities of epibiotic and internal SFS samples also exhibited significant differences. Epibiotic SFS samples were dominated by the bacterial lineages of Sulfurovaceae, Desulfobulbaceae, Flavobacteriaceae, and Campylobacteraceae. While in the internal SFS samples, the genus Candidatus Thiobios, affiliated with the Chromatiaceae, was the most dominant bacterial lineage. Furthermore, the core microbial communities of all samples, which accounted for 78 ∼ 92% of sequences, were dominated by Chromatiaceae (27 ∼ 49%), Sulfurovaceae (10 ∼ 35%), Desulfobulbaceae (2 ∼ 7%), and Flavobacteriaceae (3 ∼ 7%) at the family level. Based on the results of random forest analysis, we also found the genera Desulfobulbus and Sulfurovum were the primary bacterial lineages responsible for the dissimilarity of microbial communities between the SFS samples collected from the Kairei and Longqi vent fields. Our results indicated that the microbial lineages involved in the sulfur cycle were the key microorganisms, playing a crucial role in the hydrothermal vent ecosystems. Our findings expand current knowledge on microbial diversity and composition in the epibiotic and internal microbial communities of SFS collected from different hydrothermal vent fields.

Structure and Connectivity of Hydrothermal Vent Communities Along the Mid-Ocean Ridges in the West Indian Ocean: A Review

To date, 13 biologically active hydrothermal vent (HTV) fields have been described on the West Indian Ocean ridges. Knowledge of benthic communities of these vent ecosystems serves as scientific bases for assessing the resilience of these ecosystems under the global effort to strike an elegant balance between future deep-sea mining and biodiversity conservation. This review aims to summarize our up-to-date knowledge of the benthic community structure and connectivity of these Indian vents and to identify knowledge gaps and key research questions to be prioritized in order to assess the resilience of these communities. The HTVs in the West Indian Ocean are home to many unique invertebrate species such as the remarkable scaly-foot snail. While distinct in composition, the macrofaunal communities of the Indian HTVs share many characteristics with those of other HTVs, including high endemism, strong zonation at the local scale, and a simple food web structure. Furthermore, Indian vent benthic communities are mosaic compositions of Atlantic, Pacific, and Antarctic HTV fauna possibly owning to multiple waves of past colonization. Phylogeographic studies have shed new light into these migratory routes. Current animal connectivity across vent fields appears to be highly influenced by distance and topological barriers. However, contrasting differences in gene flow have been documented across species. Thus, a better understanding of the reproductive biology of the Indian vent animals and the structure of their population at the local scale is crucial for conservation purposes. In addition, increased effort should be given to characterizing the vents’ missing diversity (at both the meio and micro-scale) and elucidating the functional ecology of these vents.

Extending the dataset of fluid geochemistry of the Menez Gwen, Lucky Strike, Rainbow, TAG and Snake Pit hydrothermal vent fields: Investigation of temporal stability and organic contribution

The Menez Gwen, Lucky Strike, Rainbow, TAG (Transatlantic Geotraverse) and Snake Pit hydrothermal vent fields on the Mid-Atlantic Ridge were revisited and resampled for geochemical investigation during the BIOBAZ 2013 and BICOSE 2014 research cruises. Geochemical analysis of the major and minor elements of the hydrothermal fluid and concentrations of gases extends and complements the existing dataset. Our results are consistent with values previously reported and fall within the range of the analytical error. This indicates that the hydrothermal vent field system has remained relatively stable over the last few decades. However, some differences were observed and (i) suggested a recent eruption at Menez Gwen, (ii) supported the occurrence of low-temperature serpentinisation in this same site, (iii) supported a change in the reaction zone or axial magma chamber (AMC) depth at Lucky Strike, (iv) an increase of the temperature at depth at Snake Pit and (v) supported the hypothesis of large seawater entrainment through the TAG hydrothermal mound. Besides, it is possible that small temporal and spatial scale processes may control a significant part of the geochemistry, owing to the fact that some variations in the data could not be interpreted. However, our investigation of the organic geochemistry represents a pioneering addition to research for Menez Gwen, Snake Pit and TAG and a much more comprehensive study for Lucky Strike and Rainbow. Concentrations for a wide variety of semi volatile organic compounds (SVOCs) were obtained for the first time at all sites. Our results showed that a great part of the total organic carbon (TOC) could not be allocated by the total SVOCs studied here, suggesting that other processes/sources of organic carbon remain to be identified. The TAG organic geochemistry seemed entirely based on thermogenic processes whereas mixed processes may occur at the other vent field. The presence of n-alkanes suggested the contribution of a low-temperature fluid at all sites. An additional high-temperature organic matter degradation component was likely present at Menez Gwen and Lucky Strike. Our results also indicated that both abiogenic and biogenic processes produced organic compounds. Therefore, we suggest that a portion of the fatty acids at Menez Gwen and polyaromatic hydrocarbons (PAHs) at Rainbow may be derived from abiogenic processes, whereas biogenic processes could be responsible for the presence of n-fatty acids (n-FAs) at Lucky Strike and Rainbow. Moreover, organic geochemistry data appeared to be helpful in understanding some inorganic processes.

Parent–child‐based navigation method of multiple autonomous underwater vehicles for an underwater self‐completed survey

AbstractThis study proposes a navigation method of multiple autonomous underwater vehicles (AUVs) where low‐cost AUVs (child AUVs) estimate their self‐states including their position, yaw angle, and horizontal moving velocity accurately thanks to a single high‐performance AUV (parent AUV) without any external support. Since the child AUVs have only simple navigational sensors, positioning performance is not enough. The parent AUV, on the other hand, can conduct accurate self‐state estimation using high‐grade navigational sensors without any external support. The parent AUV supports the child AUVs through all processes of deployment, surveying, recovery, and surfacing. This study focuses on the accurate positioning method for the phase of the surveying. The child AUVs have acoustic devices for positioning and communication or cameras; hence, they build a parent‐centered wireless positioning and communication network and can also estimate their self‐states as accurately as the parent AUV, thanks to the positioning relative to the parent AUV. Field experiments with AUVs were conducted in a deep sea (hydrothermal vent field) and a shallow area. We confirmed that long‐range navigation could be achieved while keeping an accuracy that is 5–10 times greater than that achieved by the child AUV alone based on state estimation using sensor data from the experiments in postprocessing. The performance of the proposed system does not depend on the child AUV's performance. We also verified that the proposed method has a certain scalability and a high‐level performance in terms of accuracy, efficiency, and cost by a comparison with other existing navigation methods.

The cabled observatory vent imaging sonar deployed on the Ocean Observatories initiative’s cabled array

The Cabled Observatory Vent Imaging Sonar (COVIS) was installed on the Ocean Observatories Initiative’s Cabled Array observatory at ASHES hydrothermal vent field on Axial Seamount in July 2018. Diffuse hydrothermal flows are identified by maps made using the standard deviation of thephase change between pings separated in time by fractions of 1 s. The results demonstrate significant influences of ocean tides and bottom currents on diffuse hydrothermal discharge. The same data are used to estimate diffuse heat flux, providing two-dimensional maps of heat flux density at a rate of one per day. Sonar data are also used to generate three-dimensional backscatter images of the buoyant plumes above major sulfide structures. These backscatter images show substantial changes in plume bending in the presence of ambient currents and potentially the variations of outflow fluxes. The intensity of acoustic backscatter decreases significantly for highly bent plumes as compared to nearly vertical plumes, reflecting enhanced mixing of plume fluids with seawater driven by ambient currents. A forward model of acoustic backscatter from a buoyancy driven plume yields a reasonable match with the observation, paving the way for inversely estimating the source heat flux of a hydrothermal plume. [Work sponsored by NSF.]

Magnetic and Gravity Surface Geometry Inverse Modeling of the TAG Active Mound

AbstractSeafloor massive sulfide deposits form in remote environments, and the assessment of deposit size and composition through drilling is technically challenging and expensive. To aid the evaluation of the resource potential of seafloor massive sulfide deposits, three‐dimensional inverse modeling of geophysical potential field data (magnetic and gravity) collected near the seafloor can be carried out to further enhance geologic models interpolated from sparse drilling. Here, we present inverse modeling results of magnetic and gravity data collected from the active mound at the Trans‐Atlantic Geotraverse hydrothermal vent field, located at 26°08′N on the Mid‐Atlantic Ridge, using autonomous underwater vehicle and submersible surveying. Both minimum‐structure and surface geometry inverse modeling methods were utilized. Through deposit‐scale magnetic modeling, the outer extent of a chloritized alteration zone within the basalt host rock below the mound was resolved, providing an indication of the angle of the rising hydrothermal fluid and the depth and volume of seawater/hydrothermal mixing zone. The thickness of the massive sulfide mound was determined by modeling the gravity data, enabling the tonnage of the mound to be estimated at 2.17 ± 0.44 Mt through this geophysics‐based, noninvasive approach.

Deep‐Sea Electric and Magnetic Surveys Over Active and Inactive Basalt‐Hosted Hydrothermal Sites of the TAG Segment (26°, MAR): An Optimal Combination for Seafloor Massive Sulfide Exploration

AbstractA GEOMAR (Kiel, Germany) research team has developed a passive electric field acquisition system for Autonomous Underwater Vehicles (AUVs) to optimize seafloor massive sulfides exploration. This sensor was made of two perpendicular and horizontal pairs of electrodes, and was successfully tested over active basalt‐hosted hydrothermal site TAG (26°N, Mid‐Atlantic Ridge) and several inactive sites in its vicinity. The resulting data underline the efficiency of combining deep‐sea electric and magnetic measurements for searching for active and inactive hydrothermal vent fields. With these datasets, it becomes possible to determine the geological nature of the targets and to constrain the characteristics of fluid circulation at depth without involving costly and invasive underwater tools such as Remotely Operated Vehicles or even manned submersibles to collect samples. Data analysis also revealed that AUV attitude variations induce distortions of the electric signal. These distortions start prevailing for dives at altitudes higher than 90 m above the seafloor, as the distance between the AUV becomes too important to guarantee that the signal produced by the geological target still dominates. To improve the acquisition system and reduce the overall noise, we discuss solutions that limit the impact of such attitude variations. These solutions consist of minor adjustments, such as masts at AUVs stern to tow damping electrodes arrays. In such configurations, we believe that deep‐sea passive electric measurements combined with high‐resolution magnetic measurements can become a highly efficient seafloor exploration tool, including for sulfide deposits associated with inactive hydrothermal systems.

Passive acoustic monitoring of hydrothermal vents at the endeavour hydrothermal vent field

Passive acoustics may provide a method for detection and long-term monitoring of hydrothermal vents. Direct measurement of vent activity can be challenging as vent plumes are often high temperature (i>300°C) and acidic. The discovery of new vent sites can also be challenging as detection of vent plumes or high-resolution seafloor mapping is required. For these reasons, remote detection and monitoring with passive acoustics can be advantageous. In this presentation, long-term passive acoustic data recorded in 2019 from an Ocean Networks Canada (ONC) bottom-mounted hydrophone in the Endeavour Hydrothermal Vent field are presented. A 6-month time window from April through October 2019 was selected for analysis, when fin whale calls were less prevalent. Statistical noise level metrics (e.g., the median) were used to reduce the influence of transient, infrequent events such as fin whale calls and ship noise, as the vent signal was expected to be relatively constant compared to these sources. Spectral analysis of the resulting time series across multiple frequency bins shows evidence of tidal-period variations in power spectral density which may be related to hydrothermal vent dynamics. Comparison is made to other nearby ONC sensors such as vent temperature, bottom pressure, and acoustic doppler current profiler measurements.

Megafauna of the German exploration licence area for seafloor massive sulphides along the Central and South East Indian Ridge (Indian Ocean).

BackgroundThe growing interest in mineral resources of the deep sea, such as seafloor massive sulphide deposits, has led to an increasing number of exploration licences issued by the International Seabed Authority. In the Indian Ocean, four licence areas exist, resulting in an increasing number of new hydrothermal vent fields and the discovery of new species. Most studies focus on active venting areas including their ecology, but the non-vent megafauna of the Central Indian Ridge and South East Indian Ridge remains poorly known.In the framework of the Indian Ocean Exploration project in the German license area for seafloor massive sulphides, baseline imagery and sampling surveys were conducted yearly during research expeditions from 2013 to 2018, using video sledges and Remotely Operated Vehicles.New informationThis is the first report of an imagery collection of megafauna from the southern Central Indian- and South East Indian Ridge, reporting the taxonomic richness and their distribution. A total of 218 taxa were recorded and identified, based on imagery, with additional morphological and molecular confirmed identifications of 20 taxa from 89 sampled specimens. The compiled fauna catalogue is a synthesis of megafauna occurrences aiming at a consistent morphological identification of taxa and showing their regional distribution. The imagery data were collected during multiple research cruises in different exploration clusters of the German licence area, located 500 km north of the Rodriguez Triple Junction along the Central Indian Ridge and 500 km southeast of it along the Southeast Indian Ridge.

The megalopal stage of the hydrothermal vent crab Austinograea rodriguezensis Tsuchida amp; Hashimoto, 2002 (Decapoda: Bythograeidae): a morphological description based on CLSM images.

The Bythograeidae is unique amongst brachyuran crab taxa as it is the only family where all 6 genera and 16 species are endemic to hydrothermal vents. During the research conducted by German Federal Institute for Geosciences and Natural Resources to identify inactive polymetallic sulphide deposits along Central and Southeast Indian Ridges, the INDEX project collected from hydrothermal vent fields 6 Bythograeidae megalopae. Entire specimens and dissected appendages were stained, mounted on slides and examined using Light Microscopy and Confocal Laser Scanning Microscopy. Additional molecular analysis using mtCOI confirmed the identification of the specimens as Austinograea rodriguezensis Tsuchida Hashimoto, 2002. The megalopal stage of A. rodriguezensis shows similarities and distinctions between the characters of two other bythograeid megalopae, Bythograea thermydron Williams, 1980, and B. microps Saint Laurent, 1984. Unlike other brachyuran megalopae, B. thermydron and A. rodriguezensis lack long serrate setae and stout serrate spines on the dactylus of the fifth pereiopod. In both species no coxal spines on the pereiopods were observed. The elliptical carapace of B. thermydron is broader than long vs longitudinally rectangular in A. rodriguezensis. This carapace shape resembles B. microps more than B. thermydron, however, the dorsal surface of B. microps carapace is densely covered in short setae vs only covered with short setae on the anterior 1/4 and the posterior 1/6 length of carapace in A. rodriguezensis. Furthermore, the amount of plumose natatory setae on the pleopods in B. microps is in total larger and more variable, than in A. rodriguezensis. Bythograeid megalopae seem quite generalized and miss specific features that reveal them distinctively as endemic vent crab. A distinction from other species is possible by observing the unique combinations of certain characters. This is the first megalopal stage description of Austinograea and the fourth within the Bythograeidae.

Deltaproteobacterium Strain KaireiS1, a Mesophilic, Hydrogen-Oxidizing and Sulfate-Reducing Bacterium From an Inactive Deep-Sea Hydrothermal Chimney.

A novel deltaproteobacterial, mesophilic, hydrogen-oxidizing, and sulfate-reducing bacterium (strain KaireiS1) was highly enriched from an inactive chimney located in the active zone of the Kairei hydrothermal vent field (Central Indian Ridge) in the Indian Ocean. Based on 16S rRNA gene analyses, strain KaireiS1 is the currently only cultured representative of a cluster of uncultured Deltaproteobacteria, positioned within the Desulfobulbaceae family, between the Desulfobulbus genus and the “Cable Bacteria.” A facultative autotrophic lifestyle of KaireiS1 is indicated by its growth in the absence of organic compounds, measurements of CO2-fixation rates, and activity measurements of carbon monoxide dehydrogenase, the key enzyme of the reductive Acetyl-CoA pathway. Apart from hydrogen, strain KaireiS1 can also use propionate, lactate, and pentadecane as electron donors. However, the highest cell numbers were reached when grown autotrophically with molecular hydrogen. Hydrogen uptake activity was found in membrane and soluble fractions of cell-free extracts and reached up to 2,981±129 nmol H2*min−1*mg−1 of partially purified protein. Commonly, autotrophic sulfate-reducing bacteria from the Deltaproteobacteria class, thriving in hydrothermal vent habitats are described as thermophiles. Given its physiological characteristics and specific isolation source, strain KaireiS1 demonstrates a previously unnoticed potential for microbial sulfate reduction by autotrophs taking place at moderate temperatures in hydrothermal vent fields.

Genomic Variation Influences Methanothermococcus Fitness in Marine Hydrothermal Systems.

Hydrogenotrophic methanogens are ubiquitous chemoautotrophic archaea inhabiting globally distributed deep-sea hydrothermal vent ecosystems and associated subseafloor niches within the rocky subseafloor, yet little is known about how they adapt and diversify in these habitats. To determine genomic variation and selection pressure within methanogenic populations at vents, we examined five Methanothermococcus single cell amplified genomes (SAGs) in conjunction with 15 metagenomes and 10 metatranscriptomes from venting fluids at two geochemically distinct hydrothermal vent fields on the Mid-Cayman Rise in the Caribbean Sea. We observed that some Methanothermococcus lineages and their transcripts were more abundant than others in individual vent sites, indicating differential fitness among lineages. The relative abundances of lineages represented by SAGs in each of the samples matched phylogenetic relationships based on single-copy universal genes, and genes related to nitrogen fixation and the CRISPR/Cas immune system were among those differentiating the clades. Lineages possessing these genes were less abundant than those missing that genomic region. Overall, patterns in nucleotide variation indicated that the population dynamics of Methanothermococcus were not governed by clonal expansions or selective sweeps, at least in the habitats and sampling times included in this study. Together, our results show that although specific lineages of Methanothermococcus co-exist in these habitats, some outcompete others, and possession of accessory metabolic functions does not necessarily provide a fitness advantage in these habitats in all conditions. This work highlights the power of combining single-cell, metagenomic, and metatranscriptomic datasets to determine how evolution shapes microbial abundance and diversity in hydrothermal vent ecosystems.

Northern Mid-Atlantic Ridge Hydrothermal Habitats: A Systematic Review of Knowledge Status for Environmental Management

Highly specialised biota occurring at hydrothermally active vents on the northern Mid-Atlantic Ridge (nMAR: from south of Iceland to the Equator) have been the subject of numerous research projects over the 36-year period since these habitats were first discovered in the region. When hydrothermal activity ceases, biota endemic to hydrothermally active habitats are lost, and a new biota colonise these sites. Little is known about the biota colonising hydrothermally inactive sulphide habitats on the nMAR, although these sites may be the target of deep-sea mining within the next decade. In this review, we seek to clarify the current knowledge of biological communities colonising hydrothermally active habitats and inactive sulphide habitats on the nMAR. To achieve this, we (1) used a systematic review process to update the species list of benthic invertebrates associated with hydrothermally active habitats, (2) conducted a regional biogeographic analysis of hydrothermally active vent fields on the nMAR, (3) undertook a comprehensive literature review to provide a descriptive account of biological communities, and (4) identified key knowledge gaps in the current understanding of nMAR hydrothermally active and inactive ecosystems. Our updated species list increases the number of benthic invertebrates recorded from hydrothermally active habitats on the nMAR to 158 taxa. Our regional biogeographic analysis separates nMAR hydrothermal vent fields into distinct clusters based on depth/latitude and chimney composition. Vent fields close to the Azores (Menez Gwen, Lucky Strike, Rainbow) formed a separate cluster from those at greater depths south of the Azores (Broken Spur, TAG, Snake Pit, Logatchev, and Ashadze-1). Moytirra, located north of the Azores, clustered separately, as did Lost City with its unique carbonate chimneys. We present detailed information on the biological communities at hydrothermally active and inactive habitats in this region, and discuss the information available on the diversity, ecosystem function, trophic relationships, connectivity, temporal variability, and resilience and recovery of these communities. Many knowledge gaps still exist, with detailed information needed on all aspects of the biological communities at hydrothermally active habitats and inactive sulphide habitats on the nMAR to understand and predict impacts from natural and human-induced disturbances in the region.

Transport of Heat by Hydrothermal Circulation in a Young Rift Setting: Observations From the Auka and JaichMaa Ja'ag' Vent Field in the Pescadero Basin, Southern Gulf of California

AbstractHeat flow measurements collected throughout the Auka and JaichMaa Ja'ag' hydrothermal vent fields in the central graben of the Southern Pescadero Basin, southern Gulf of California, indicate upflow of hydrothermal fluids associated with rifting dissipate heat in excess of 10 W/m2 around faults that have a few kilometers in length. Paradoxically, longer faults do not show signs of venting. Heat flow anomalies slowly decay to background values of ∼2 W/m2 at distances of ∼1 km from these faults following an inverse square‐root distance law. We develop a near‐fault model of heat transport in steady state for the Auka vent field based on the fundamental Green's function solution of the heat equation. The model includes the effects of circulation in fracture networks, and the lateral seepage of geothermal brines to surrounding hemipelagic sediments. We use an optimal fitting method to estimate the reservoir depth, permeability, and circulation rate. Independently derived constraints for the model, indicate the heat source is at a depth of ∼5.7 km; from the model, permeability and flow rates in the fracture system are ∼10−14 m2 and 10−6 m/s, respectively, and ∼10−16 m2 and 10−8 m/s in the basin aquitards, respectively. Model results point to the importance of fault scaling laws in controlling sediment‐hosted vent fields and slow circulation throughout low permeability sediments in controlling the brine's chemistry. Although the fault model seems appropriate and straightforward for the Pescadero vents, it does seem to be the exception to the other known sediment‐hosted vent fields in the Pacific.