Dietary Hydrolysate Research Articles

Польза элиминационной диеты из гидролизованной рыбы и рисового крахмала для диагностики неблагоприятных пищевых реакций у кошек: открытое клиническое исследование

Background: diagnosis of adverse food reaction (AFR) is based on an eight week elimination diet (ED) and is confirmed by relapse upon re-challenge with the previously fed diet. Hydrolysed EDs are commonly used for this purpose. Objective: to evaluate the commercially available hydrolysed fish protein and rice starch ED Farmina UltraHypo (FUH) for the diagnosis of feline AFR. Animals: thirty-two nonseasonally pruritic cats. Materials and methods: pruritus was assessed with a new dual Visual Analog Scale, lesions with the Scoring Feline Allergic Dermatitis scale and Quality of Life with a validated questionnaire on days 0 and 56. Short-acting corticosteroids or oclacitinib were permitted during the first six weeks. Cats showing 50% pruritus and/or lesional improvement were separately challenged with their prior diet, fish and rice. Cats not responding to the study diet were fed another hydrolysed diet for two months. Results: 25 cats completed the ED: four dropped out due to vomiting and/or diarrhoea, one owing to low palatability and two were lost to follow-up. In 17 cats, pruritus improved by >50% and these underwent dietary challenges. Of these, nine reacted to their prior diet and/or fish and/or rice and were diagnosed with AFR, while eight did not relapse (and a diagnosis of AFR was considered to be doubtful). Of the eight cats in which pruritus did not improve, four underwent a second ED with no improvement. Conclusion and clinical importance: FUH may be a useful ED for the diagnosis of feline AFR, even in cats reacting to fish or rice

Camel whey protein hydrolysate diet mitigates alkaline stress–induced biochemical disorders and restores the target of rapamycin, MAPK pathway, and autophagy-related gene expression in Nile tilapia

Camel whey protein hydrolysate diet mitigates alkaline stress–induced biochemical disorders and restores the target of rapamycin, MAPK pathway, and autophagy-related gene expression in Nile tilapia

Cholesterol-Lowering and Antioxidant Effects of Pork-Liver Protein Hydrolysate in Otsuka Long-Evans Tokushima Fatty Rats.

In this study, we investigated the effects of a porcine liver protein hydrolysate (PLH) diet on lipid metabolism in Otsuka Long-Evans Tokushima Fatty (OLETF) rats, a model of type II diabetes. OLETF rats (20-wk-old males) were pair-fed with either a PLH diet containing 20% PLH or a casein diet for 14 wk. Dietary PLH significantly lowered serum cholesterol and phospholipid concentrations, mainly by decreasing low-density lipoprotein and high-density lipoprotein fractions. Fecal cholesterol was significantly increased in the PLH diet group; however, the total bile acid concentration in the feces was not significantly different between the groups. In addition, the PLH diet significantly decreased serum thiobarbituric acid reactive substance concentrations. These results suggest that dietary PLH exerts hyperlipidemic and antioxidant effects, indicating that it is a novel functional food ingredient.

The effects of hydrolyzed protein on macronutrient digestibility, fecal metabolites and microbiota, oxidative stress and inflammatory biomarkers, and skin and coat quality in adult dogs.

Research on protein hydrolysates has observed various properties and functionalities on ingredients depending on the type of hydrolysate. The objective of this study was to evaluate the effects of hydrolyzed chicken protein that was incorporated into diets on digestibility, gut health, skin and coat health, oxidative stress, and intestinal inflammation markers in healthy adult dogs. Five complete and balanced diets were manufactured: (1) CONd: 25% chicken meal diet; (2) 5% CLHd: 5% chicken liver and heart hydrolysate plus 20% chicken meal diet; (3) CLHd: 25% chicken liver and heart hydrolysate diet; (4) 5% CHd: 5% chicken hydrolysate plus 20% chicken meal diet; (5) CHd: 25% chicken hydrolysate diet. A replicated 5 × 5 Latin square design was used which included 10 neutered adult Beagles. Each of the 5 periods consisted of a 7-d washout time and a 28-d treatment period. All diets were well accepted by the dogs. Fecal butyrate concentration was higher while fecal isovalerate and total phenol/indole were lower in dogs fed CLHd than CONd (P < 0.05). Dogs fed CHd had higher fecal immunoglobulin A concentration when compared with CLHd (P < 0.05); however, both groups were comparable to the CONd. There was no difference among groups in serum cytokine concentrations, serum oxidative stress biomarkers, or skin and coat health analyses (P > 0.05). Fecal microbiota was shifted by CLHd with higher abundance in Ruminococcus gauvreauii group as well as lower Clostridium sensu stricto 1, Sutterella, Fusobacterium, and Bacteroides when compared with CONd (P < 0.05). There was also a difference in beta diversity of fecal microbiota between CLHd and CHd (P < 0.05). In conclusion, chicken protein hydrolysate could be incorporated into canine extruded diets as a comparable source of protein to traditional chicken meal. The test chicken protein hydrolysates showed the potential to support gut health by modulating immune response and microbiota; however, functional properties of protein hydrolysates are dependent on inclusion level and source.

196 The Effect of Hydrolyzed Protein on Fecal Microbiota in Adult Dogs

Abstract Research on protein hydrolysates has observed various properties and functionalities of these ingredients depending on the type of hydrolysate. The objective of this study was to evaluate the effect of hydrolyzed chicken protein that was incorporated into diets on gut health in healthy adult dogs. Five complete and balanced treatment diets were manufactured: 1) CONd: chicken meal diet; 2) 5% CLHd: 5% substitution of chicken liver hydrolysate of chicken meal diet; 3) CLHd: chicken liver hydrolysate diet; 4) 5% CHd: 5% substitution of chicken hydrolysate of chicken meal diet; and 5) CHd: chicken hydrolysate diet. A 5×5 Latin square design was used which included 10 neutered adult beagles. Each of the 5 periods consisted of a 7-d washout time and a 28-d treatment period. All diets were well accepted by the dogs. For fecal metabolites, there was greater fecal butyrate concentration as well as reduced isovalerate, 4-ethylphenol, and indole in dogs fed CLHd than CONd (P &amp;lt; 0.05). Regarding the overall microbiota composition, Firmicutes, Bacteriodota, Fusobacteriota, Actinobacteriota, and Proteobacteria were the top 5 most abundant phyla. At the class level, samples from CHd group had greater relative abundances of Bacteriodia and Fusobacteriia as well as less Bacilli. On the other hand, CLHd group had greater abundances of Bacilli and Actinobacteria with less Fusobacteriia. At the level of order, CHd group had greater Bacteriodales and Fusobacteriales while CLHd group had greater Bifidobacteriales. At the family level, CHd group showed a greater abundance of Muribaculaceae and a decreased abundance of Bifidobacteriaceae when compared with the other groups. Alternatively, CLHd group demonstrated a greater abundance of Bifidobacteriaceae together with reduced abundance of Fusobacteriaceae, Muribaculaceae, Sutterrellaceae, and Ruminococcaceae. Fecal microbiota was shifted by CLHd with greater differential abundance in Ruminococcus gauvreauii group as well as reduced Clostridium sensu stricto 1, Sutterella, Fusobacterium, and Bacteroides compared with CONd (P &amp;lt; 0.05). There was also a difference in beta diversity of fecal microbiota between CLHd and CHd (P &amp;lt; 0.05). However, no difference was observed in alpha diversity among treatment groups (P &amp;lt; 0.05). Fecal butyrate concentration was negatively correlated with the genera Blautia, Phascolarctobacterium, Sutterella, Faecalibacterium, and Bacteriodes. Conversely, Allobaculum and Bifidobacterium were positively correlated with butyrate concentration. In conclusion, the test chicken protein hydrolysates may have potential to support gut health by modulating microbiota.

PSIX-6 The Effects of Hydrolyzed Protein on Macronutrient Digestibility, Fecal Metabolites, Oxidative Stress and Inflammatory Biomarkers, and Skin and Coat Quality in Adult Dogs

Abstract Research on protein hydrolysates has observed various properties and functionalities of these ingredients depending on the type of hydrolysate. The objective of this study was to evaluate the effects of hydrolyzed chicken protein that was incorporated into diets on digestibility, gut health, skin and coat health, oxidative stress, and inflammation in healthy adult dogs. Five complete and balanced treatment diets were manufactured: 1) CONd: chicken meal diet; 2) 5% CLHd: 5% substitution of chicken liver hydrolysate of chicken meal diet; 3) CLHd: chicken liver hydrolysate diet; 4) 5% CHd: 5% substitution of chicken hydrolysate of chicken meal diet; and 5) CHd: chicken hydrolysate diet. A 5×5 Latin square design was used which included 10 neutered adult beagles. Each of the 5 periods consisted of a 7-d washout time and a 28-d treatment period. All diets were well accepted by the dogs. There was greater (P &amp;lt; 0.05) fecal butyrate (226.8 μmol/g DMB) concentration as well as decreasedr isovalerate (6.8 μmol/g DMB), 4-ethylphenol (1.4 μmol/g DMB), and indole (0.7 μmol/g DMB) in dogs fed CLHd than CONd (149.3, 10.8, 2.5, and 1.5 μmol/g DMB, respectively). Doges fed CHd had greater (P &amp;lt; 0.05) fecal immunoglobulin A concentration (2.86 mg/g) when compared with CLHd (0.91 mg/g); however, both groups were comparable to the control. There was no difference among groups in serum cytokine concentrations, serum oxidative stress biomarkers, or skin and coat health analyses (P &amp;gt; 0.05). In conclusion, chicken protein hydrolysate could be incorporated into canine extruded diets as a comparable source of protein to traditional chicken meal. The test chicken protein hydrolysates may have potential to support gut health by modulating immune response and fermentative activity; however, more research is needed to confirm the effect.

Raspberry ketone feeding makes Queensland fruit fly, Bactrocera tryoni (Froggatt) more vulnerable to desiccation but not starvation.

Queensland fruit fly (Qfly) males exhibit accelerated sexual maturation when their diet is supplemented with raspberry ketone (RK) for 48 hours following emergence which is beneficial for Sterile Insect Technique (SIT) operation. The present study tests whether RK supplementation makes Qfly more vulnerable to starvation or desiccation. Flies were fed for 48 hours with yeast hydrolysate and sugar diet (1:3) that contained 0% RK (control), 1.25% RK (low dose) or 5% RK (high dose) to test subsequent vulnerability to starvation and desiccation. RK feeding decreased body weight and water content in males and increased lipid levels in both sexes before exposure to any stress treatment. Under nutritional stress, flies fed the low RK dose, but not the high RK dose, had higher survival than controls. Under desiccation stress, flies fed both the low and high RK doses had lower survival than the controls. Body weight, water content and lipid reserves at death were all affected by RK dose when under nutritional stress, but not when under desiccation stress. In the absence of stress, body weight at death was higher than controls in flies provided the high RK dose and lipids were lower than controls in flies provided the low RK dose. Feeding with RK makes Qflies more vulnerable to desiccation but not starvation. In most conditions, it is expected that the disadvantage of increased desiccation vulnerability would be outweighed by the benefits of accelerated sexual maturation in RK-fed young adult Qflies. This article is protected by copyright. All rights reserved.

Salmon hydrolysate as a protein source for Atlantic salmon; prion content and effects on growth, digestibility and gut health

Feeding unhydrolyzed protein to the same species from which it came from have caused prion disease in some mammals, but this has not been observed in fish. In this study, we characterized a salmon protein hydrolysate (FPH) for prion content and investigated how partially replacing fish meal with salmon hydrolysate, affected growth, digestibility and gut health of Atlantic salmon (Salmo salar). The salmon hydrolysate was analysed for prion content by shotgun and targeted proteomics, but no prions were found. Atlantic salmon post smolts were fed one of the following: a control diet containing 30% fish meal (FM), a 20% FM with 9% salmon hydrolysate diet (FPH-09) or a 10% FM with 18% salmon hydrolysate diet (FPH-18). Salmon fed the hydrolysate diets had higher specific growth rate over the first 25 days. The FPH-18 diet contained more digestible proteins and amino acids than the FM diet, and the ash digestibility increased linearly with the FPH content, indicating increased mineral uptake. Histological analysis of midgut and hindgut did not reveal any significant differences between the FM and FPH-18 diets, and the intestines were assessed to be normal and healthy. These results imply that salmon hydrolysate is a promising novel feed ingredient for Atlantic salmon, due to its excellent amino acid profile and high digestibility.

A Novel Approach in the Development of Larval Largemouth Bass Micropterus salmoides Diets Using Largemouth Bass Muscle Hydrolysates as the Protein Source.

This study's objectives were to determine the effect of Largemouth Bass (LMB) muscle hydrolysates obtained using same-species digestive enzymes and the degree of LMB muscle hydrolysis when included in the first feeds of growth performance and survival, skeletal development, intestinal peptide uptake, and muscle-free amino acid composition of larval LMB. LMB muscle was mixed with digestive enzymes from adult LMB, and hydrolyzed for 1.5, 3, and 6 h, respectively. Five diets were produced, the intact diet containing non-hydrolyzed muscle and four diets with 37% muscle hydrolysate inclusion. Those diets were characterized by their level of each hydrolysate (presented as a ratio of 1.5, 3, and 6 Ts hydrolysates): 1:1:1, 1:3:6, 1:3:1, 6:3:1 for diets A, B, C, and D, respectively. To account for gut development, one group of larval LMB was fed a weekly series of diets B, C, and D to provide an increasing molecular weight profile throughout development. This group was compared against others that received either; (1) diets D, C, and B; (2) diet A; or (3) intact diet. The initial inclusion of the hydrolysates significantly improved the total length of the larval LMB; however, neither the hydrolysate inclusion nor the series of dietary molecular weight profiles improved the overall growth of larval LMB. The inclusion of hydrolysates significantly decreased the occurrence of skeletal deformities. The degree of hydrolysis did not have a significant effect on the parameters measured, except for intestinal peptide uptake, which was increased in the group that received the most hydrolyzed diet at the final time of sampling. The lack of overall growth improvement suggests that while the hydrolysates improve the initial growth performance, further research is necessary to determine the optimal molecular weight profile, hydrolysate inclusion level, and physical properties of feeds for larval LMB.

The anti-anxiety/depression effect of a combined complex of casein hydrolysate and γ-aminobutyric acid on C57BL/6 mice.

In view of a series of adverse side effects of drugs for anxiety/depression on the market at present, it is imminent to extract and develop novel anti-anxiety and depression drugs from plants and proteins (like casein hydrolysate) as adjuncts or substitutes for existing anti-anxiety and depression drugs. Consequently, this study investigated the improvement of the anxiety/depression function by the compound of casein hydrolysate and γ-aminobutyric acid (GABA) (casein hydrolysate: GABA = 4:1; CCHAA) on mice induced by chronic restraint stress-corticosterone injection. Animal experiments revealed that oral gavage administration of CCHAA significantly reversed the anxiety/depression-like behaviors. Compared to the model control group, body weights were increased after treatment with CCHAA groups [1.5, 0.75 mg/(g⋅d)]. As a diagnostic index of anxiety and depression, we assessed GABA and 5-HT levels in response to CCHAA ingestion. The GABA and 5-HT levels were increasingly enhanced by the CCHAA diet. In addition, histopathological changes in the hippocampus CA3 region of the anxious/depressed mice were also alleviated after the treatment with the CCHAA. Thus, the casein hydrolysate and GABA formula diets may induce beneficial effects on the mice with anxiety/depression.

A Preliminary Study of Modulen IBD Liquid Diet in Hospitalized Dogs with Protein-Losing Enteropathy.

Simple SummaryModulen IBD is an oral liquid food that results in remission rates similar to immunosuppressive drugs in children with inflammatory bowel disease. This diet has not been previously investigated in dogs. We aimed to describe the use of Modulen IBD in hospitalized dogs with inflammatory protein-losing enteropathy (PLE) when combined with whey powder and a multivitamin/mineral blend to ensure this was complete and balanced for dogs. Five dogs hospitalized for PLE that had an esophagostomy feeding tube placed were eligible and prospectively enrolled. All dogs received Modulen IBD without concurrent immunosuppressive drugs and tolerated tube feedings. All dogs had resolution of anorexia, three had stable or improved serum albumin concentrations, four had improved or normalized serum globulin concentrations, and four dogs had improved or normalized serum cholesterol concentrations 2–3 days after initiating the diet. In conclusion, the Modulen IBD liquid diet was well-tolerated in-hospital and resolved anorexia in all dogs and helped to improve selected biochemical parameters in some dogs. Further studies are needed to assess the long-term effects of feeding this diet to dogs with inflammatory PLE. Modulen IBD is an enteral liquid diet that can induce remission rates similar to glucocorticoids in children with inflammatory bowel disease. The Modulen IBD liquid diet has not been previously investigated in dogs. Our study aimed to describe the use of the Modulen IBD liquid diet in hospitalized dogs with inflammatory protein-losing enteropathy (PLE), including its tolerance and effects on appetite and gastrointestinal signs, and laboratory parameters during hospitalization. Of the 14 dogs hospitalized for PLE that had an esophagostomy feeding tube placed at the time of endoscopy, 5 were eligible and prospectively enrolled. The Modulen IBD liquid diet was supplemented with whey powder isolate and a multivitamin/mineral blend to ensure the diet was complete and balanced for canine adult maintenance and had a macronutrient profile desirable for PLE. All five dogs tolerated tube feedings with the Modulen IBD liquid diet, allowing an increase of 75 to 100% of the resting energy requirement (RER) by day 3 to 4. The diet was administered without glucocorticoid in all five dogs. All five of these dogs had a resolution of anorexia allowing the voluntary intake of a commercial hydrolyzed protein diet prior to the use of glucocorticoids. Of these five dogs, three (60%) had stable or improved serum albumin concentrations (median % increase: 10.3, range: 0–31.1), four (80%) had improved or normalized serum globulin concentrations (median % increase: 12.9, range: 5.1–66.2) and four (80%) had improved or normalized serum cholesterol concentrations (median % increase: 31.5, range: 4.8–63) 2–3 days after initiating the diet. However, there were no significant differences in these selected biochemical parameters pre- and post-feeding with the diet (p > 0.080). In conclusion, the Modulen IBD liquid diet, fed via an esophagostomy feeding tube was well-tolerated in-hospital and resolved anorexia in all dogs and helped to improve selected biochemical parameters in some dogs. Further studies are needed to assess the long-term effects of feeding this diet on the rate of serum albumin increase and remission in dogs with inflammatory PLE.

A recommended amount of hydrolyzed protein improves physiological function by regulating gut microbiota in aged mice

Dietary proteins play a critical role in maintaining the health of elderly people. Although experts recommend that elderly people consume more protein, a high-protein diet may add to the burden of elderly people with degraded digestion and absorption functions. The effects of a normal or high-protein diet and those of a whole or hydrolyzed protein diet on bone and muscle health and gastrointestinal function were evaluated in aged female C57BL/6J mice. The hydrolyzed protein diet with 14.7% protein energy ratio (HNP) contributed to the maintenance of weight and an increase in bone and muscle mass. Further, the overall aging situation was improved by the consumption of this diet. However, the hydrolyzed protein diet with 21.3% protein energy ratio (HHP) increased the levels of LPS, IL-6, IL-10, and TNF-α in serum. Additionally, the small intestine structure was damaged, and the goblet cell number was decreased in the HHP and whole protein with 21.3% protein energy ratio (HP) groups. The relative abundances of Streptococcus and Peptococcus were decreased while that of Bifidobacterium was increased in HNP group compared with the whole protein with 14.7% protein energy ratio (NP) and HP groups. Undigested proteins entering the intestine may cause undesirable changes in gut microbiota, which adversely affect the aging body in NP and HP groups. In summary, hydrolyzed proteins are more advisable than untreated dietary protein in aged mice. This study aimed to provide guidance for daily diet for elderly people, and provide additional information to industry in order to guide their future food development.

Gall bladder ejection fractions in dogs investigated for chronic altered appetite: 14 cases (2015-2017).

To determine if gall bladder dysmotility occurs in dogs investigated for chronic altered appetite and to determine if gall bladder dysmotility warrants further investigation as a contributing factor to altered appetite. Case series of dogs investigated for chronic gastrointestinal disease. Gastrointestinal clinical signs were assessed before and after a 6-week hydrolysed protein diet. Gall bladder ejection fractions were determined at the end of the 6-week hydrolysed protein diet as part of an investigation that included a full blood cell count, biochemistry, abdominal X-rays and ultrasound. The gall bladder ejections fraction results of dogs with normal appetite were compared to dogs with general inappetence and dogs with diurnal inappetence in the morning. In this retrospective case series of 14 dogs, altered appetite was the most frequent and persistent clinical sign associated with chronic gastrointestinal disease. Nine dogs had suboptimal gall bladder function and this occurred in dogs with, and without, gravity-dependent biliary sludge. Gall bladder function and volumes of dogs in this study were comparable to those of dogs with nongravity-dependent gall bladder sludge or gall bladder mucoceles in other studies. There was an observable difference in gall bladder ejection fractions between groups defined by appetite but no statistically significant difference was present. Small sample sizes meant the effect size was large. Gall bladder dysmotility and distension can occur in the absence of gall bladder sludge and mucocoeles in younger dogs. Gall bladder dysmotility requires further investigation as a potential contributing factor to altered appetite in dogs.

Anti-Atherogenic Effect of 10% Supplementation of Anchovy (Engraulis encrasicolus) Waste Protein Hydrolysates in ApoE-Deficient Mice.

Fish protein consumption exerts beneficial metabolic effects on human health, also correlating with a decreased risk for cardiovascular disease. Fish waste contains high amount of proteins and utilization may offer the opportunity for generating compounds advantageous for human health. Especially, fish waste protein hydrolysates beneficially influence pathways involved in body composition, exerting anti-inflammatory and antioxidant activities, making their potential supplementation in human disorders of increased interest. This study assessed the effect of a 10% (w/w) anchovy waste protein hydrolysate (APH) diet for 12 weeks in reducing atherosclerosis in ApoE−/− mice, through histological and immunohistochemical methods. In addition, monitoring of plaque development was performed, using high-frequency ultrasound and magnetic resonance imaging. Overall, the APH diet attenuated atherosclerotic plaque development, producing a regression of arterial lesions over time (p < 0.05). Twelve weeks on an APH diet had an anti-obesity effect, improving lipid metabolism and reducing hepatic enzyme activity. A significant reduction in plaque size and lipid content was observed in the aortic sinus of APH-fed mice, compared to the control (p < 0.001), whereas no differences in the extracellular matrix and macrophage recruitment were observed. Supplementation of APH significantly attenuates atherosclerosis in ApoE−/− mice, exerting a lipid-lowering activity. The opportunity to use fish waste protein hydrolysates as a nutraceutical in atherosclerosis is worthy of future investigations, representing a low cost, sustainable, and nutritional strategy with minimal environmental impact.

Comparative evaluation of feeding effects of A1 and A2 cow milk derived casein hydrolysates in diabetic model of rats

• The study was designed with Type-1 diabetes as the backdrop. During the trial period of 60 days, none of the rats became pre-diabetic due to respective hydrolysate feeding. • Anti-diabetic potential of indigenous cow milk (A2) derived casein hydrolysates was not indicated as it failed to offer resistance to diabetes induction using streptozotocin. • Feeding of A1 or A2 cow milk derived casein hydrolystaes did not cause any deteriorative effect on the blood biochemical profile and histopathology of major organs like heart, liver and kidney. The present study was designed to test diabetogenic activity of A1 and A2 casein hydrolysates and other related pathological conditions, in murine species. 6 weeks old adult male wistar rats were selected and first divided into 3 groups (n = 12) as Control, A1CH and A2CH were fed with respective A1 and A2 casein hydrolysate diets for 50 days. On 51st day, each group was divided into 2 sub groups (n = 6) and diabetes was induced in one subgroup of each group using Streptozotocin, which resulted in 6 sub groups. The fasting blood glucose level and other biochemical, diabetic specific parameters and histopathological findings post sacrifice, did not differ significantly (p > 0.05) among control, A1CH and A2CH groups. Whereas, the diabetic rats showed significant (p < 0.05) increase in the fasting blood glucose levels, blood biochemicals and significant (p < 0.05) decrease in body weight, HDL, insulin and C-peptide levels when compared to healthy rats. However, there was no significance (p < 0.05) difference in various biochemical, diabetic specific parameters and histopathological findings among the diabetic groups viz. , STZ, A1CH + STZ, A2CH + STZ except the significantly ( p < 0.05) higher levels of cholesterol and LDL noticed in A1CH + STZ as compared to STZ group. The results suggest that A1 and A2 casein hydrolysates did not have any marked effect on various health parameters when fed for 60 days to the healthy rats and the STZ induced rats for the last 10 days of the study.

Quantifying myelin content in brain tissue using color Spatial Light Interference Microscopy (cSLIM).

Deficient myelination of the brain is associated with neurodevelopmental delays, particularly in high-risk infants, such as those born small in relation to their gestational age (SGA). New methods are needed to further study this condition. Here, we employ Color Spatial Light Interference Microscopy (cSLIM), which uses a brightfield objective and RGB camera to generate pathlength-maps with nanoscale sensitivity in conjunction with a regular brightfield image. Using tissue sections stained with Luxol Fast Blue, the myelin structures were segmented from a brightfield image. Using a binary mask, those portions were quantitatively analyzed in the corresponding phase maps. We first used the CLARITY method to remove tissue lipids and validate the sensitivity of cSLIM to lipid content. We then applied cSLIM to brain histology slices. These specimens are from a previous MRI study, which demonstrated that appropriate for gestational age (AGA) piglets have increased internal capsule myelination (ICM) compared to small for gestational age (SGA) piglets and that a hydrolyzed fat diet improved ICM in both. The identity of samples was blinded until after statistical analyses.

The use of hydrolysed diets for vomiting and/or diarrhoea in cats in primary veterinary practice.

To describe responses of cats prescribed a hydrolysed diet with or without concurrent medication for chronic vomiting and/or diarrhoea of undetermined aetiology. Anonymised records of 512,213 cats under UK veterinary care in 2016 from the VetCompass database were searched using relevant terms for hydrolysed diets. The records of 5000 (90%) of 5569 cats with evidence of receiving a hydrolysed diet were randomly reviewed for gastrointestinal indication, prior and concurrent medication and response after hydrolysed dietary intervention. A poor response was defined as evidence of receiving antibiotic or glucocorticoid treatment for vomiting/diarrhoea at visits after the onset of the diet or death from gastrointestinal signs for at least 6months follow-up. Of 977 cats prescribed a hydrolysed diet for chronic vomiting/diarrhoea, 697 (71%) were first prescribed the diet without concurrent antibiotics or glucocorticoids while 280 (29%) first received the diet with these medications. Thirty-four per cent of cats in the former group and 61% in the latter had a poor response. Cats older than 6 years and cats prescribed antibiotic and/or glucocorticoid for vomiting/diarrhoea before and concurrently with the diet had higher odds of poor response. Although variations in our observations may reflect severity of signs or prescribing habits of primary-care veterinary surgeons, our study suggests there is merit in trialling a hydrolysed diet first as a sole therapy in cats with chronic vomiting/diarrhoea when diagnostic investigations do not reveal a cause, before resorting to antibiotic and/or glucocorticoid therapy for cases that respond poorly.

Dietary Fiber-Bound Polyphenols Modify Canine Fecal Endocannabinoid Levels and Improve Stool Firmness

Abstract Objectives Not all dietary fibers are equal in effect. Fiber may entrap bioactive polyphenols (PP), enabling them to bypass digestion and arrive in the colon. We documented effects of fiber combinations on stool firmness and fecal metabolomics in dogs fed a hydrolyzed meat diet, hypothesizing that fiber-bound PP would deliver these bioactives to the colon, improve stool firmness and alter the fecal metabolome. Methods Dogs were group housed, provided daily exercise in outdoor grassy runs, had access to natural light that varied with season and were fed to healthy body weight. Health was monitored through clinical and physical indices. Fecal collections were under IACUC protocols. Fibers were measured by AOAC and PP by Folin-Ciocalteu methods. Nine foods using a base of hydrolyzed chicken and cornstarch together allowed parsing of the effects of fiber-bound PP-containing food ingredients (active blend; AB). Control food had cellulose as a fiber source, AB added pecan, citrus, beet, flax and cranberry. Cellulose, psyllium and pumpkin were explored as supplements to AB. Additionally, other fiber sources were tried alone and in combination: pea fiber, tomato pomace, maize dextrin, silica dioxide inulin, fructooligosaccharide. Dogs (n = 6) offered a diet for 10 days, with daily stool scoring on day 6–10 for firmness on an ordinal scale. A sample for fecal metabolomics by LC-MS was frozen within one hour of defecation on day 10. Linear Mixed Modeling was used to examine diet effects. Results The AB was 65% fiber and 20% PP by weight, with PP mostly in bound form. Stool firmness was highest from dogs fed the AB-containing foods; inclusion of cellulose was not required for maximal stool score. AB-containing foods increased fecal fiber-derived sugars, PP and microbial products of PP metabolism; this was not apparent from foods devoid of PP sources. Across foods, inclusion of whole flax increased fecal levels of long chain 2-acylglycerol endocannabinoids while decreasing levels of very long chain acylethanolamide endocannabinoids. Colonic microbial lipolysis may have contributed to the appearance of 1-acyl and 2-acylglycerols. Conclusions It would appear that provision of microbiome-nourishing fibers, along with fiber-bound PP, may differentially alter acylglycerol and ethanolamide endocannabinoid levels and increase stool firmness. Funding Sources Hill's Pet Nutrition.

Alterations in some physiological processes of Bactrocera oleae Rossi (Diptera: Tephritidae) following pyridalyl treatment

Pyridalyl is one of the recently discovered insecticides which has shown toxicity against several insect pests with slight- or non-lethal effects against natural enemies. Cytotoxicity and prevention of protein synthesis have been reported as pyridalyl mode of action. In the current study, adults of Bacterocera oleae, as a major pest of olive, were fed on protein hydrolysate diet containing LC50 concentration of pyridalyl to determine the possible mode of action through gene expression and amino acid profile. Results showed that treated flies had significantly higher gene expressions of cytochrome P450, insulin receptor-like, antioxidant enzymes (catalase, peroxidase, superoxide dismutase) and the enzymes involved in cell death; caspase1, caspase3, caspase8 and caspase9 compared to control. Moreover, determination of amino acid profile in the whole body of both control and pyridalyl-treated B. oleae revealed the significant higher amounts of glutamic acid, glutamine, histidine, threonine, citrulin, arginine, taurine, alanine, tyrosine, α-amino butric acid, methionine and isoleucine in the treated adults while the control flies had the higher amounts of serine, glycine and tryptophane. No significant differences were found in the amounts of asparagine and phenylalanine between control and treatments. These results are in accordance with earlier findings on mode of action of pyridalyl through activation of cytochrome P450 system and alterations in protein synthesis machinery although here other mechanisms were also reported including activation of insulin receptor and antioxidant enzymes. Moreover, it is the first report on cytotoxicity through apoptosis with engagement of caspases.

Hydrolyzed diets may stimulate food-reactive lymphocytes in dogs.

Hydrolyzed proteins are often prescribed for dogs with food hypersensitivity in food elimination programs. However, the potential of these diets to stimulate lymphocyte-mediated hypersensitivity is currently unknown. In this study, two commercially available hydrolyzed diets for dogs, D-1 (Aminopeptide Formula Dry, Royal Canin Japon, Tokyo, Japan), and D-2 (Canine z/d Ultra Dry, Hill’s-Colgate (Japan) Ltd., Tokyo, Japan), were analyzed to identify residual proteins or peptides, as well as activated helper T-lymphocyte reactions in dogs with suspected food hypersensitivity. Proteins and peptides with molecular weights >1 kDa (majority 1.5–3.5 kDa) were detected in both diet extracts with sodium dodecyl sulfate polyacrylamide gel electrophoresis, and size exclusion chromatography. When peripheral blood mononuclear cells (PBMC’s) from 316 dogs with suspected food allergies were cultured with hydrolyzed diet extracts, flow cytometry analysis revealed detectable levels of CD25low helper T-lymphocytes stimulated by D-1 and D-2 in 91 of 316, (28.8%), and 75 of 316 (23.7%) samples, respectively. These data indicated that the extracts contained proteins or peptides large enough to activate the lymphocytes. The percentages of CD25low helper T-lymphocytes stimulated by D-1 and D-2 extracts increased to 38.7% and 29.6%, respectively, in 186 of the original 316 samples (186/316, 58.9%), also reactive to poultry-related antigens. Thus, both poultry-related antigens, and D-1 and D-2 diet extracts may activate helper T-lymphocytes. These results demonstrate that hydrolyzed diets may contain proteins that stimulate helper T-lymphocytes, and may not be effective for treating all dogs with food hypersensitivity.