Hemicellulosic Hydrolysate Research Articles

Optimisation of alkaline pretreatment of spent coffee grounds for microbial oil production by Cryptococcus curvatus

In a world of finite resources, the transition to a sustainable post-fossil resources economy is essential. Within this concept, spent coffee grounds (SCGs) could be used for the production of bio-based chemicals, biopolymers and value-added products. Lipids and phenolics were initially extracted from SCGs. Alkaline pretreatment of the remaining SCGs solids has been optimised in this study via Central Composite Design to maximise lignin removal and overall sugar to polysaccharide conversion yield. The optimal SCGs pretreatment conditions were 0.06% (w/v) NaOH and 99.5 °C resulting in 35.7% lignin removal at 60 min pretreatment duration with low glucan and hemicellulose losses (ca. 2.5%). Enzymatic hydrolysis of the remaining SCGs alkaline pretreated solids resulted in 79.4% glucan, 74.5% mannan and 54.8% hemicellulose hydrolysis yield. The SCGs hydrolysate was used as fermentation feedstock in bioreactor cultures of Cryptococcus curvatus resulting in 36.1 g/L microbial oil with 0.25 g/g yield and 0.41 g/(L∙h) productivity. The total dry weight reached 62.7 g/L corresponding to 57.5% (w/w) intracellular lipid content. The microbial oil was rich in oleic acid (55.6%) followed by palmitic acid (18.9%) and stearic acid (14.0%). The proposed process led to the production of 7.81 g microbial oil per 100 g antioxidant- and oil-free SCGs.

Production of hemicelluloses sugars, cellulose pulp, and lignosulfonate surfactant using corn stalk by prehydrolysis and alkaline sulfite cooking

Full components utilization of corn stalk was investigated by the combine processes of prehydrolysis and alkaline sulfite cooking. Prehydrolysis was employed to remove hemicelluloses prior to cooking. Glucose yield of 76.8 g/kg and xylose yield of 118.4 g/kg corn stalks were obtained from the hydrolysis of hemicelluloses at 150 °C for 3 h. Significantly, cellulose was largely preserved during prehydrolysis as indicated by the pulp yield from 54 % to 62 % from the alkaline sulfite cooking. Paper sheet showed superior physical strength over hardwood pulp, e.g. tensile index 97.4 N·m/g, tear index 6.6 mN·m2/g and burst index 5.2 kPa·m2/g. In addition, lignin in spent liquor of alkaline sulfite cooking was recovered as lignosulfonate surfactant which reduced the surface tension of water to 38.2 mN/m at a concentration of 15 g/L. The combined processes of prehydrolysis and alkaline sulfite cooking enables full utilization of cellulose, hemicelluloses, and lignin, and therefore has great potential to industrial application.

Mapping potential solvents for inhibitors removal from sugarcane bagasse hemicellulosic hydrolysate and its impact on fermentability

Mapping potential solvents for inhibitors removal from sugarcane bagasse hemicellulosic hydrolysate and its impact on fermentability

Enhancing the compost maturation of deer manure and corn straw by supplementation via black liquor

In this paper, the relationship between black liquor and microbial growth, enzymatic secretion and humus formation in composting was studied. The results showed that black liquor inoculation is an effective way to promote fermentation process. After black liquor inoculation, the abundance of Corynebacterium, Aequorivita, and Pedobacter, which have the catalase and oxidase activity, has been significantly increased. The enzymatic activity of alkaline phosphatase, catalase, peroxidase and invertase was 40 mg/(g·24h), 6.5 mg/(g·20 min), 13 100 mg/(g·24h), and 6100 mg/(g·24h) respectively at day 18. Humic acid and fulvic acid concentration was 12 g/kg and 11 g/kg which is higher than that of the treatments of no black liquor inoculation. The results suggested that black liquor inoculation was beneficial to indigenous microorganisms reproduce efficiently, then the secretion of enzymes related to cellulose, hemicellulose, and lipid hydrolysis, and the formation of humic substances.

Micro- and nano-scale mechanisms of enzymatic treatment on the interfacial behaviors of sisal fiber reinforced bio-based epoxy resin

Theoretically, sisal fiber (SF) is an ideal reinforcing material for green composites due to its high specific strength and specific modulus. However, the weak interface performance between SFs and resin affects the mechanical properties of composites. Therefore, the surface modification of SFs was carried out by enzymatic treatment to enhance the interfacial properties between SFs and bio-based epoxy resin in this work. It was found that the interfacial strength was effectively increased by 108% after the SF was treated with xylanase and pectinase for 6 h. The experiment study showed that the enzymatic treatment mainly caused the hydrolysis and cleavage of pectin and hemicellulose of SFs, which increased the surface grooves and surface roughness of SFs. The further finite element analysis proved that the mechanical interlocking effect at the nano-scale induced by surface roughness of SFs was the primary reason for the increase of interfacial strength between SFs and bio-based epoxy resin. Enzymatic treatment provides a green and large-scale production method for interface modification of SFs reinforced composites, which also can be used for the other bast fiber reinforced composites.

Characterization of the recombinant GH10 xylanase from Trichoderma orientalis EU7-22 and its synergistic hydrolysis of bamboo hemicellulose with α-glucuronidase and α-L-arabinofuranosidase

Here the recombinant GH10 xylanase (HoXyn10) was investigated for biochemical characteristics and synergistic effect with α-glucuronidase (AnGus67) and α-L-arabinofuranosidase (AnAxh62A) on extracted Maso bamboo hemicellulose (BCH) to produce xylooligosaccharides (XOS). The GH10 endo-β-1, 4-xylanase from Trichoderma orientalis EU7–22 is heterologously expressed in Pichia pastoris GS115. The crude HoXyn10 showed three bands on the SDS-PAGE profile and one of the proteins was N-glycosylated. The optimum pH and temperature of HoXyn10 were pH= 6.0 and 65 °C, respectively. It showed a wide range of pH stability and remained more than 80% of optimal activity at pH4.5–8.0 after holding at 40 °C for 60 min. HoXyn10 was highly stable at 50 °C without substrate and the residue xylanase activities were retained 97% and 83% after incubating for 2 h and 18 h, respectively. HoXyn10 displayed high activities and almost the same catalytic efficiency to oat-spelt xylan (OSX), Beechwood xylan (BWX) and wheat arabinoxylan (WAX). Notably, the HoXyn10 exhibited apparent activity on Avicel, but showed no activity on CMC-Na. The hydrolytic products from oligosaccharides (X3-X6) by HoXyn10 were all xylose (X1) and xylobiose (X2). The results from FT-IR and NMR showed the backbone of extracted BCH was (1→4)-linked β-D-xylopyranose and the main side chains were α-L-arabinofuranose residues and 4-O-methyl-D-glucuronic acid residues. Based on the characterized structure, when BCH was synergistically hydrolyzed with HoXyn10, AnGus67 and AnAxh62A using appropriate strategy, the yield of xylooligosaccharide (XOS) and xylose were 50.3% and 14.7%. The results indicated HoXyn10 might be a promising tool for various hemicellulose hydrolysis and Maso bamboo hemicellulose is a promising source for XOS production.

The Brazilian Amazonian rainforest harbors a high diversity of yeasts associated with rotting wood, including many candidates for new yeast species.

This study investigated the diversity of yeast species associated with rotting wood in Brazilian Amazonian rainforests. A total of 569 yeast strains were isolated from rotting wood samples collected in three Amazonian areas (Universidade Federal do Amazonas-Universidade Federal do Amazonas [UFAM], Piquiá, and Carú) in the municipality of Itacoatiara, Amazon state. The samples were cultured in yeast nitrogen base (YNB)-d-xylose, YNB-xylan, and sugarcane bagasse and corncob hemicellulosic hydrolysates (undiluted and diluted 1:2 and 1:5). Sugiyamaella was the most prevalent genus identified in this work, followed by Kazachstania. The most frequently isolated yeast species were Schwanniomyces polymorphus, Scheffersomyces amazonensis, and Wickerhamomyces sp., respectively. The alpha diversity analyses showed that the dryland forest of UFAM was the most diverse area, while the floodplain forest of Carú was the least. Additionally, the difference in diversity between UFAM and Carú was the highest among the comparisons. Thirty candidates for new yeast species were obtained, representing 36% of the species identified and totaling 101 isolates. Among them were species belonging to the clades Spathaspora, Scheffersomyces, and Sugiyamaella, which are recognized as genera with natural xylose-fermenting yeasts that are often studied for biotechnological and ecological purposes. The results of this work showed that rotting wood collected from the Amazonian rainforest is a tremendous source of diverse yeasts, including candidates for new species.

Oleaginous yeasts for biochemicals, biofuels and food from lignocellulose-hydrolysate and crude glycerol.

Microbial lipids produced from lignocellulose and crude glycerol (CG) can serve as sustainable alternatives to vegetable oils, whose production is, in many cases, accompanied by monocultures, land use changes or rain forest clearings. Our projects aim to understand the physiology of microbial lipid production by oleaginous yeasts, optimise the production and establish novel applications of microbial lipid compounds. We have established methods for fermentation and intracellular lipid quantification. Following the kinetics of lipid accumulation in different strains, we found high variability in lipid formation even between very closely related oleaginous yeast strains on both, wheat straw hydrolysate and CG. For example, on complete wheat straw hydrolysate, we saw that one Rhodotorula glutinis strain, when starting assimilatingD-xylosealso assimilated the accumulated lipids, while a Rhodotorula babjevae strain could accumulate lipids onD-xylose.Two strains (Rhodotorula toruloides CBS 14 and R. glutinis CBS 3044) were found to be the best out of 27 tested to accumulate lipids on CG. Interestingly, the presence of hemicellulose hydrolysate stimulated glycerol assimilation in both strains. Apart from microbial oil, R. toruloides also produces carotenoids. The first attempts of extraction using the classical acetone-based method showed that β-carotene is the major carotenoid. However, there are indications that there are also substantial amounts of torulene and torularhodin, which have a very high potential as antioxidants.

Strategy to reduce acetic acid in sugarcane bagasse hemicellulose hydrolysate concomitantly with xylitol production by the promising yeast Cyberlindnera xylosilytica in a bioreactor.

The yeast Cyberlindnera xylosilytica UFMG-CM-Y309 has been identified as a promising new xylitol producer from sugarcane bagasse hemicellulosic hydrolysate (SCHH). However, SCHH pretreatment process generates byproducts, which are toxic to cell metabolism, including furans, phenolic compounds, and carboxylic acids, such as acetic acid, typically released at high concentrations. This research aims to reduce acetic acid in sugarcane hemicellulose hydrolysate concomitantly with xylitol production by yeast strain Cy. xylosilytica UFMG-CM-Y309 in a bioreactor by strategically evaluating the influence of volumetric oxygen transfer coefficient (kLa) (21 and 35h-1). Experiments were conducted on a bench bioreactor (2L volumetric capacity) at different initial kLa values (21 and 35h-1). SCHH medium was supplemented with rice bran extract (10gL-1) and yeast extract (1gL-1). Cy. xylosilytica showed high xylitol production performance (19.56gL-1), xylitol yield (0.56gg-1) and, maximum xylitol-specific production rate (μpmáx 0.20 gxylitol·g-1h-1) at kLa value of 21h-1, concomitantly slowing the rate of acetic acid consumption. A faster acetic acid consumption (100%) by Cy. xylosilytica was observed at kLa of 35h-1, concomitantly with an increase in maximum cellular growth (14.60gL-1) and reduction in maximum xylitol production (14.56gL-1 and Yp/s 0.34gg-1). This study contributes to pioneering research regarding this yeast performance in bioreactors, emphasizing culture medium detoxification and xylitol production.

Sequential production of hydrogen and methane using hemicellulose hydrolysate from diluted acid pretreatment of sugarcane straw

Sugarcane straw is not effectively used in the industry currently. However, sugarcane straw hemicellulose hydrolyzate (HH) application as raw material for H2 and CH4 production is a promising alternative. In this work, the two-stage anaerobic digestion (TS-AD) approach led to 37.86 mLH2/L.h and 40.12 mLCH4/L.d, while the single-stage anaerobic digestion (SS-AD) generated 46.11 mLCH4/L.d. Hence, the two-stage process was energetically favorable than the single-stage by approximately 33%. Additionally, the comparison with standard medium (composed of glucose, xylose, and arabinose) applied as raw material indicated that although hydroxymethylfurfural and furfural from HH were not responsible for the decrease in H2 production, they extended the adaptive phase of methanogenic archaea during the methanogenesis. Hemicellulose hydrolysate is an attractive raw material for two-stage anaerobic digestion.

Evaluation of Preparation and Detoxification of Hemicellulose Hydrolysate for Improved Xylitol Production from Quinoa Straw.

Quinoa straw is rich in hemicellulose, and it could be hydrolyzed into xylose. It is a promising energy resource alternative that acts as a potential low-cost material for producing xylitol. In this study, quinoa straw was used as a substrate subjected to the hydrolysis of dilute sulfuric acid solution. Based on the production of xylose and inhibitors during hydrolysis, the optimal conditions for the hydrolysis of hemicellulose in quinoa straw were determined. Detoxification was performed via activated carbon adsorption. The optimal detoxification conditions were determined on the basis of major inhibitor concentrations in the hydrolysate. When the addition of activated carbon was 3% at 30 °C for 40 min, the removal of formic acid, acetic acid, furfural, and 5-HMF could reach 66.52%, 64.54%, 88.31%, and 89.44%, respectively. In addition to activated carbon adsorption, vacuum evaporation was further conducted to perform two-step detoxification. Subsequently, the detoxified hydrolysate was used for xylitol fermentation. The yield of xylitol reached 0.50 g/g after 96 h of fermentation by Candida tropicalis (CICC 1779). It is 1.2-fold higher than that obtained through the sole vacuum evaporation method. This study validated the feasibility of xylitol production from quinoa straw via a biorefinery process.

Cellulases, hemicellulases and ligninolytic enzymes: mechanism of action, optimal processing conditions and obtaining value-added compounds in plant matrices

This review article has focused on the enzymatic hydrolysis (HE) of plant matrices (MV), the mechanisms of action of cellulases, hemicellulases and ligninases; the optimal process conditions most used to obtain products of interest; as well as some factors that reduce catalytic activity. Based on the enzymatic mechanism, a compendium of the applications on different substrates is elaborated, with the idea of reorienting the current application of the hydrolysis of MV (biofuels) and providing an alternative for its use and obtaining compounds of added value. For example, the extraction of phenolic compounds with antioxidant capacity, of xylooligosaccharides and aromatic phenolic compounds from the hydrolysis of cellulose, hemicellulose and lignin, respectively.

Xylitol production by different yeasts: Kinetic study and biosynthesis from cashew apple bagasse hydrolysate

AbstractXylitol is a polyol with sweetening and anti‐cariogenic properties and is highly relevant to the food and pharmaceutical industries. This work evaluates xylitol production by three yeasts (Candida tropicalis, Kluyveromyces marxianus CCA510, and Kluyveromyces marxianus ATCC 36907) from different carbon sources: D‐xylose (medium xylose [MX]) and D‐xylose plus D‐glucose (medium xylose and glucose [MXG]). The potential of xylitol production from hemicellulose hydrolysate of cashew bagasse was evaluated. In MX medium, K. marxianus CCA510 was the strain that produced higher xylitol concentration (17.04 g · L−1). However, K. marxianus ATCC 36907 and C. tropicalis produced 13.22 and 9.54 g · L−1, respectively. On the other hand, in MXG medium, probably due to the presence of glucose as a carbon source, lower xylitol production was observed for all microorganisms: the CCA510 strain produced 13.30 g · L−1 of xylitol, while C. tropicalis and ATCC 36907 produced 11.42 and 0.64 g · L−1, respectively. Additionally, the production of ethanol as a secondary product was also noted. According to the results of the kinetic study, xylitol formation is associated with the growth and consumption of substrate (xylose), which is a typical behaviour of a primary metabolite for the three yeasts. Furthermore, the strain K. marxianus CCA510 was able to produce xylitol from cashew apple bagasse hydrolysate (CABH), evidencing its potential for use in bioprocesses related to biorefinery. In view of the results reported here, it was possible to clarify in detail the kinetics of xylitol production by the yeast strains evaluated, which had the ability to produce xylitol from CABH, providing added value to this agro‐industrial residue.

The influence of NaCl during hydrothermal carbonization for rice husk on hydrochar physicochemical properties

NaCl was used to solubilize organic matter from rice husk (RH), followed by subsequent hydrothermal carbonization (HTC) to explore the catalysis role of Cl− to components of lignocellulose during the HTC due to the interactions of NaCl (Na+ and Cl−) with –OH group would promote cellulose and hemicellulose hydrolysis to improve carbonization. Ultrasonic pretreatment was also used to enhance the process. The results demonstrated that ultrasonic pretreatment could effectively destroy the structure of RH, and enhanced the hydrolysis of RH during the HTC. The ultrasonic pretreatment could benefit dehydration and inhibit decarboxylation during the HTC for its free radical role. The addition of NaCl could improve the hydrolysis, dehydration and decarboxylation performance and the NaCl would be absorbed on the surface of hydrochar. It was found that NaCl would promote the removal of hemicellulose and enhance the depolymerization of lignin to form monomers during the HTC, resulting in crack formation on the surface of hydrochar for RH. The results would give more help to understand the role about Cl− during HTC of lignocellulose and other organic waste.

Production, Physicochemical and Structural Characterization of a Bioemulsifier Produced in a Culture Medium Composed of Sugarcane Bagasse Hemicellulosic Hydrolysate and Soybean Oil in the Context of Biorefineries

Biosurfactants are amphipathic molecules, biodegradable, with reduced toxicity. They can be synthesized by fermentative processes from oleaginous compounds and agro-industrial by-products. In this context, the present study describes the production and the physical, chemical, and structural characterization of the bioemulsifier secreted by the yeast Scheffersomyces shehatae 16-BR6-2AI in a medium containing hemicellulosic sugarcane bagasse hydrolysate combined with soybean oil. The bioemulsifier was produced in Erlenmeyer flasks and isolated; then, the physicochemical and structural characterization of the formed molecule was carried out. The following fermentation parameters were obtained: YX/S = 0.45, YP/S = 0.083, and productivity of 0.076 g/L/h. The bioemulsifier was found to be a polymer containing 53% of carbohydrates, 40.92% of proteins, and 6.08% of lipids, respectively. The FTIR spectrum confirmed the presence of functional groups such as amides, amines, and carbonyls. The bioemulsifier was stable over a range of temperature (−20 °C to 120 °C), salinity (1–15%), and pH (2–12). It was observed that the biomolecule has a better emulsifying action in organic solvents with a non-polar character. Therefore, this biomolecule is a potential substitute for synthetic surfactants and can be used in different applications.

Биоконверсия целлюлозосодержащего сырья. Ферментативный гидролиз целлюлозы (обзор литературы)

Lignocellulose is the most abundant biopolymer on earth. The hydrolysis of lignocellulose to fermentable sugars is a prerequisite for its successful use as a substrate for the large-scale production of value-added industrial products. Enzymatic hydrolysis of lignocellulose is carried out by cellulases, hemicellulases, and ligninases in synergy or individually. The review describes the enzymatic hydrolysis of cellulose, hemicellulose, and lignin. Information on solid-phase and submerged microorganisms fermentation used to obtain cellulases was given. Methods for increasing the level of production and activity of cellulases were characterized. The industrial application of cellulases, including for the production of bioethanol, was described.

Hydrothermal pretreatment of Eucalyptus by-product and refining of xylooligosaccharides from hemicellulosic hydrolysate

Xylooligosaccharides (XOS) are highly effective food bioproducts of value to the prebiotic market. The use of hydrothermal pretreatment (HP) on Eucalyptus by-product (EB) under suitable conditions can allow to obtain valuable XOS-rich hemicellulosic hydrolysate (HH). This work aimed to design an efficient methodology to refine XOS-rich HH, boosting the development of new applications for natural XOS obtained from EB. The approach includes the refining of XOS-rich HH, obtained after the HP, in which the HH was separated and fractioned using a liquid–liquid extraction (LLE) process with organic solvents, a vacuum evaporation, and an integrated downstream processing using the two operations. Both refining processes were efficient in removing unwanted components from HH, mainly total aromatics, including hydroxymethylfurfural (HMF) and furfural. LLE performance was maximized by adjusting the volumetric ratio of ethyl acetate (EtOAc). This operation was highly efficient for removing the aromatic fraction, with a removal efficiency of 63–82% of the total aromatics and only a small loss of XOS (of circa 15%). The use of vacuum evaporation to refine the HH allowed to remove 55.4% of total aromatics with an XOS average loss of 23.8%. The combination of vacuum evaporation and LLE (1:1 vol ratio of HH: EtOAc) was beneficial for the removal of total aromatics (75.7%), but negatively affected the content of XOS in the refined HH (average loss of XOS of 29.3%). Anyway, the integrated platform resulted in a purification index of 75.5%, which represents an increase of 34.1% when compared to raw HH. This work demonstrated that the use of LLE using EtOAc, vacuum evaporation and an integrated processing using both operations are effective and simple approaches to refine XOS-rich HH, especially for the removal of aromatics contaminants, presenting a potential to improve the industrial hemicellulose biorefining processes.

RNA-seq based transcriptomic analysis of the non-conventional yeast Spathaspora passalidarum during Melle-boinot cell recycle in xylose-glucose mixtures

The Melle-Boinot is a promising process for second-generation ethanol production by xylose-fermenting yeasts. However, the impact of this process on the physiology of the non-conventional yeast Spathaspora passalidarum during second-generation ethanol production remains unclear. Therefore, we performed a transcriptomic analysis of S. passalidarum to determine the differences and global responses of differentially expressed genes (DEGs) during five fed-batch fermentations with cell recycle. A cycle-to-cycle metabolic reprogramming was observed resulting in an increase in ethanol yield (32%), volumetric productivity (33%), and titer (33%); and an expressive decrease of 94% of xylitol production. A broad set of pathways operated synergically such as fatty acid metabolism, N-glycan biosynthesis, glyoxylate and dicarboxylate metabolism, oxidative phosphorylation, glutathione metabolism and sulfur metabolism, indicating those as important mechanisms for cell recycling adaptations due to increased ethanol concentration, long-term ethanol exposure and osmotic stress. Together these results suggest that cellular energy was redirected towards the production of cell wall components enabling S. passalidarum cells to thrive on consecutive recycles. Furthermore, these results are instrumental to guide genetic-engineering efforts of xylose-fermenting yeasts to improve the productiveness and feasibility of renewable energy production from lignocellulosic biomass and hemicellulosic hydrolysates.

Two carriages for efficient furfural production from biomass: Rational design of porous biochar catalyst and clever utilization of butyrolactone-water medium

In the conversion of lignocellulosic biomass to furfural, a low yield is often obtained due to the natural degradation-resistant structure of the biomass and the high reactivity of the furfural molecule. However, in this study, the efficient conversion of biomass to furfural (90.5 % furfural yield) was achieved by working in the coordination of both carbon-based solid acid catalyst and water-butyrolactone co-solvent system using bagasse pith biomass as feedstock. The solid acid catalyst prepared by loaded sulfonic acid groups and Fe3+ on biochar showed a high specific surface area and well-developed pore channels, which can facilitate the breakdown of the solid structure of bagasse pith and the adequate hydrolysis. Meanwhile, by adjusting the volume ratio of the co-solvent system, the water in the system can not only foster the hemicellulose hydrolysis in bagasse pith but also facilitate the dehydration of xylose to form furfural. Furthermore, it enables the butyrolactone in the system to form a protective layer around the furfural molecule in time to avoid the degradation condensation reaction of the furfural molecule in the acidic system. Eventually, the 90.5 % furfural yields were obtained at water-butyrolactone volume ratio, reaction temperature, and reaction time were 1:29, 190, and 3 h, respectively.

Improving 3-hydroxypropionic acid production in E. coli by in silico prediction of new metabolic targets

3-Hydroxypropionic acid (3-HP) production from renewable feedstocks is of great interest in efforts to develop greener processes for obtaining this chemical platform. Here we report an engineered E. coli strain for 3-HP production through the β-alanine pathway. To obtain a new strain capable of producing 3-HP, the pathway was established by overexpressing the enzymes pyruvate aminotransferase, 3-hydroxyacid dehydrogenase, and L-aspartate-1-decarboxylase. Further increase of the 3-HP titer was achieved using evolutionary optimizations of a genome-scale metabolic model of E. coli containing the adopted pathway. From these optimizations, three non-intuitive targets for in vivo assessment were identified: L-alanine aminotransferase and alanine racemase overexpression, and L-valine transaminase knock-out. The implementation of these targets in the production strain resulted in a 40% increase in 3-HP titer. The strain was further engineered to overexpress phosphoenolpyruvate carboxylase, reaching 0.79 ± 0.02 g/L of 3-HP when grown using glucose. Surprisingly, this strain produced 63% more of the desired product when grown using a mixture of glucose and xylose (1:1, C-mol), and gene expression analysis showed that the cellular adjustment to consume xylose had a positive impact on 3-HP accumulation. Fed-batch culture with xylose feeding led to a final titer of 29.1 g/L. These results reinforce the value of computational methods in strain engineering, enabling the design of more efficient strategies to be assessed. Moreover, higher production of 3-HP under a sugar mixture condition points towards the development of bioprocesses based on renewable resources, such as hemicellulose hydrolysates.