The hydrothermal systems of Kamchatka are unique natural laboratories where minerals are formed under the natural conditions at the present time. Elucidation of the role of bacteria, algae, and diatoms from thermal waters and bacterial mats in the geochemical transformation of rocks has attracted the special attention of researchers. The involvement of microorganisms in the processes of precipitation and formation of new mineral forms has been studied for a long time and has remained an essential piece of evidence for their active role in the functioning of thermal ecosystems [1]. In the present work, we studied certain characteristics of the waters of the Viluchinskaya hydrothermal system. We analyzed the chemical composition of the water, the total number of bacteria and the proportion of microorganisms in the aquatic microbial community that exhibited enzymatic activity. Actual evidence of the formation of amorphous precipitates of silicon, iron, and calcium on the surface of bacterial cells is also given. Water samples were collected in March and October, 2002 from hydrogeological wells nos. 1 and 2, located on the right bank of the Spokoinyi stream, from natural thermal outlets 1 and 2 of the travertine cupola and from the arsenical spring, located on the travertine terrace along the right bank of the Vilucha river. The physicochemical parameters of the water (water temperature, pH, and concentration of dissolved oxygen) were determined under field conditions. The chemical composition of the water was analyzed by methods of colorimetry, a combination of photometry and atomic absorption and atomic emission spectrophotometry, mass-spectrometry (ICP-MS), and X-ray fluorescence analysis. To assay the total number of bacteria (TNB), the samples were prepared as described earlier [2]. The numbers of bacteria exhibiting enzymatic activity (BEA) were determined with 5-carboxyfluorescein diacetate (CFDA); its intracellular decomposition by esterases results in the formation of a fluorochrome carboxyfluorescein. CFDA (Molecular Probes Inc.) was added directly during collection of thermal water samples up to the final concentration of 0.1 mM [3]. The samples of autoclaved thermal water served as a negative control. Cell counts were performed with an epifluorescent microscope (Nikon EFD-3; digital camera system: Nikon COOLPIX E995, Japan) equipped with a set of appropriate color filters at 100 × magnification. The average and the standard deviation were assayed by the results of cell counts in 10‐20 random microscope fields in all filters. The portion of EAB was expressed as a percentage of the TNB. The samples were prepared for scanning electron microscopy (SEM) by the method of cryophilic drying [4]. The samples were analyzed by the SEM equipped with energy spectrometer (SEM: JEOLJSM-5200LV; EDS: Philips-EDAX PV9800 STD, Japan). The grids for transmission microscopy were fastened on the slides with a carbonic suspension to assess the morphological diversity of microorganisms of thermal waters. The slides were incubated under the natural conditions for a day, fixed with 2.5% glutaric aldehyde, dried in the air, and analyzed with a JEOL 2000EX transmission microscope (Japan) at accelerating voltage 80‐120 kV. All samples were characterized by low concentrations of dissolved oxygen, which did not exceed 1.63 mg/ml (at well 1, the temperature 57°C ), and high temperatures ( 57–74°C ). All samples had pH values close to neutral (from 6.85 to 7.75). The waters varied
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