Hydrocarbon Degradation Rates Research Articles

Remediation of diesel contaminated soil by using activated persulfate with Fe3O4 magnetic nanoparticles: effect and mechanisms.

In this study, Fe3O4 magnetic nanoparticles (Fe3O4 MNPs) were assessed for their ability to enhance the activity of persulfate (PS). Various controlling factors including PS dosages, initial pH, water-soil ratio, ratio of Fe2+, and Fe3O4 MNPs to PS were considered in both the Fe2+/PS system and the Fe3O4 MNPs/PS system. Results showed that the Fe3O4 MNP-activated PS system exhibited high processing efficiency owing to the gradual release of Fe2+. This process occurred in a wide pH range (5-11), attributed to the synergistic action of sulfate radicals (SO4-·) and hydroxyl radicals (OH·) under alkaline conditions, effectively mitigating soil acidification. The ratio of Fe3O4 MNPs to PS and water-soil ratio significantly influenced the degradation rate with the highest petroleum hydrocarbon degradation rate exceeding 80% (82.31%). This rate was 3.1% higher than that achieved by the Fe2+/PS system under specific conditions: PS dosage of 0.05 mol/L, Fe3O4 MNPs to PS ratio of 1:10, water-soil ratio of 2:1, and initial pH of 11. Meanwhile, oxidant consumption in the Fe3O4 MNPs/PS system was halved compared to the Fe2+/PS system due to the slow release of Fe2+ and less ineffective consumption of SO4-·. Mechanistically, the possible degradation process was divided into three parts: the initial chain reaction, the proliferating chain reaction, and the terminating chain reaction. The introduction of Fe3O4 MNPs accelerated the degradation rate of pentadecane, heneicosane, eicosane, tritetracontane, and 9-methylnonadecane.

Long‐term evaluation of hydrocarbon degradation rates within the source zone under natural and enhanced attenuation for site closure purposes

AbstractDespite addressing the cleanup of petroleum hydrocarbon contamination plumes in groundwater, challenges with site closure persist due to the ongoing long‐term contaminant release from source zones. Thus, source zone monitoring even in the presence of residual light nonaqueous‐phase liquid (LNAPL) is mandated by many countries' regulatory agencies before site closure. In this study, four independent controlled‐release sites, each containing a particular fraction of ethanol in gasoline, that is, 10%, 24%, 25%, and 85% (v/v), were subjected to monitored natural attenuation (MNA) alone (E24 and E85), and with nitrate (E25) and sulfate (E10) biostimulation at the vicinity of the source zone. To the best of the authors' knowledge, this is the longest controlled gasohol release monitoring study performed to date at the source zone for site closure (nearly 18 years of monitoring). Both natural source zone depletion and biostimulation were found to reduce benzene concentrations to acceptable remediation target levels according to environmental regulatory councils. Benzene biodegradation rates (λs) were one order of magnitude higher than those reported in the literature for diluted concentrations in groundwater plumes. The decay rate of benzene varied between 0.45 and 1.75/year and was strongly influenced by the mass of ethanol. Compared to biostimulation with nitrate (half‐life of 1.52 years), MNA alone resulted in faster benzene removal rates (half‐life of 0.96 years). Anaerobic biostimulation with sulfate had negligible effects on benzene, toluene, ethylbenzene, and xylene (o‐, m‐, and p‐xylene [BTEX]) biodegradation compared to natural attenuation alone. The highest ethanol concentration (E85) led to faster benzene removal (with a half‐life of 0.40 years), which was even higher than MNA under lower ethanol concentrations (E24). Benzene half‐life was 2.2‐fold slower in the site with the lowest ethanol (E10) compared to E85, indicating that the negative effects of ethanol on BTEX biodegradation appeared to be short‐lived and may need to be re‐evaluated over the long term. Benzene decay rates were approximately six times slower in the source zone than the rates obtained for groundwater plumes, emphasizing the importance of targeting the LNAPL after plume retreat. The study's findings can assist practitioners in better predicting the lines of evidence for BTEX bioremediation and remediation cleanup times at a lower cost in the presence of ethanol, as well as providing guidance for determining whether biostimulation is necessary to expedite source zone remediation for site closure.

Effect of biomass adsorbent on non-thermal plasma activated treatment of oil-based drilling cutting: Residual toxicity assessment

The oil-based drilling cutting (OBDC), which contain petroleum hydrocarbons produced during the oil and shale gas exploration process, are considered toxic solid waste for the environment. In this study, we introduce a promising technique by coupling non-thermal dielectric barrier discharge (DBD) plasma with biomass adsorbents (wheat stalk, straw fiber, or rice husk) to remove toxic compounds from OBDC. The results show the plasma treatment can achieve up to 90.9% degradation rate of total petroleum hydrocarbons (TPH). The introduction of 3 wt% of biomass adsorbent exhibited a significant enhancement of the energy efficiency (up to 21.9% increase using rice husk). Importantly, toxicity evaluations, such as the corn seed germination test, demonstrate a substantial reduction in the toxicity of drilling cuttings after plasma treatment, with seed germination and growth rates (using straw fiber) comparable to uncontaminated soil. Adding biomass adsorbents can also enhance the hydrophilicity of treated OBDC, increasing the presence of HCOO–, CH3HCOO–, NO3- species which further contributes to the safety and fertility of treated OBDC. Coupling non-thermal DBD plasma discharge with biomass adsorbents presents an efficient and environmentally friendly choice for the environmental treatment of OBDC in deep shale gas development.

Unraveling the functional instability of bacterial consortia in crude oil degradation via integrated co-occurrence networks.

Soil ecosystems are threatened by crude oil contamination, requiring effective microbial remediation. However, our understanding of the key microbial taxa within the community, their interactions impacting crude oil degradation, and the stability of microbial functionality in oil degradation remain limited. To better understand these key points, we enriched a crude oil-degrading bacterial consortium generation 1 (G1) from contaminated soil and conducted three successive transfer passages (G2, G3, and G4). Integrated Co-occurrence Networks method was used to analyze microbial species correlation with crude oil components across G1-G4. In this study, G1 achieved a total petroleum hydrocarbon (TPH) degradation rate of 32.29% within 10 days. Through three successive transfer passages, G2-G4 consortia were established, resulting in a gradual decrease in TPH degradation to 23.14% at the same time. Specifically, saturated hydrocarbon degradation rates ranged from 18.32% to 14.17% among G1-G4, and only G1 exhibited significant aromatic hydrocarbon degradation (15.59%). Functional annotation based on PICRUSt2 and FAPROTAX showed that functional potential of hydrocarbons degradation diminished across generations. These results demonstrated the functional instability of the bacterial consortium in crude oil degradation. The relative abundance of the Dietzia genus showed the highest positive correlation with the degradation efficiency of TPH and saturated hydrocarbons (19.48, 18.38, p < 0.05, respectively), Bacillus genus demonstrated the highest positive correlation (21.94, p < 0.05) with the efficiency of aromatic hydrocarbon degradation. The key scores of Dietzia genus decreased in successive generations. A significant positive correlation (16.56, p < 0.05) was observed between the Bacillus and Mycetocola genera exclusively in the G1 generation. The decline in crude oil degradation function during transfers was closely related to changes in the relative abundance of key genera such as Dietzia and Bacillus as well as their interactions with other genera including Mycetocola genus. Our study identified key bacterial genera involved in crude oil remediation microbiome construction, providing a theoretical basis for the next step in the construction of the oil pollution remediation microbiome.

Remediation of Petroleum-contaminated Soil by Highly Efficient Oil-degrading Bacteria and Analysis of Its Enhancement Mechanism

A 120-day in situ remediation of oil-contaminated soil was carried out by using highly efficient oil-degrading bacteria. The effects of bio-enhanced remediation and changes in soil physicochemical properties and enzyme activities were investigated. Combined with metagenomic sequencing and bioinformatics analysis, the strengthening mechanism was revealed. The results showed that compared with the blank control group (Ctrl), the degradation rate of total petroleum hydrocarbons in the bioremediation group (Exp-BT) was significantly increased, reaching 81.23%. During enhanced bioremediation by highly efficient oil-degrading bacteria, the pH of the soil was stable, the oxidation capacity of the system was improved, and the electrical conductivity was in the range suitable for agricultural activities. Lipase and dehydrogenase maintained high activity during repair. In addition, the analysis of the initial contaminated soil (B0), the highly efficient oil-degrading bacteria obtained from domestication (GZ), and the soil samples after bioremediation (BT) in the obtained samples showed that, at the phylum level, the total proportion of Proteobacteria and Actinobacteria increased by 17.1%. At the genus level, the abundance of Nocardioides, Achromobacter, Gordonia, and Rhodococcus increased significantly. The species and function contribution analysis of COG and KEGG proved that the above bacterial genera had important contributions to the degradation of petroleum hydrocarbons. A high abundance of petroleum hydrocarbon-related metabolic enzymes and five petroleum hydrocarbon-related degradation genes was found in the soil after remediation:alkM, tamA, rubB, ladA, and alkB. The analysis showed that the introduction of the exogenous petroleum hydrocarbon-degrading bacteria group enhanced the metabolic activity of microorganism-related enzymes and the expression of corresponding functional genes.

Degradation of crude oil by the microbial populations of Lagos and Ologe lagoon waters

Introduction: hydrocarbon contamination of surface waters as a result of anthropogenic activity poses threat to ecosystems and counter their beneficial uses. Some indigenous microbial communities have the potential to purify such waters unaided. Aims: To show the biodegradative potential of microbial communities in Lagos and Ologe Lagoons during minimal pollution with crude oil. Materials and Methods: The total heterotrophic bacteria and hydrocarbon utilising bacterial and fungal populations were estimated from Lagos and Ologe lagoon water samples contaminated with 1% crude oil over 42-day incubation period by plate count and vapour-phase transfer techniques. Residual hydrocarbons were determined by Gas chromatography. Results: The predominant bacterial genera identified from the lagoons include Enterobacter, Klebsiella and Proteus, while Escherichia, and Morganella. Aspergillus and Mucor were the predominant fungal genera in both waters. The hydrocarbon degradation rate in the Lagos Island microcosm was 65.391±0.370 mg/l/d, degradation rate constant 0.05±0.01 /d, half-life 9.559±0.093 /d and percentage degradation of 95.315 ± 0.134. Corresponding values in the Ologe water were 61.190± 8.542 mg/l/d, 8.725 ±0.389 /d, 0.055±0.003/d and 96.345±0.488 respectively. There was almost complete disappearance of the various fractions of the oil in the two samples. The microbial communities from both lagoons effectively utilised majority of the hydrocarbon fractions after 42 days where 66.890±1.075 and 100±000 were recorded for benzene, toluene had 100±000 percent degradation, anthracene 96.755±0.119 and 99.726±0.026, and pristane had 91.674±0.222 and 99.943±0.015 while phytane had 96.44±0.058 and 99.670±0.104 respectively. Conclusion: Efficient biodegradation of moderate contamination crude oil could be achieved by indigenous microbial flora present in Lagos and Ologe lagoon waters.

Influence of Spent Lubricating Oil Spiked Compost on Microbial Counts and Hydrocarbon Degradation Rate in Soils

This study evaluated the influence of organic fertilizers produced from spent lubricating oil (SLO) spiked aerobic composting technique on hydrocarbon degradation rate in soils. The compost windrows (Ft2 and Ft4), consisting of kitchen and agricultural wastes, were spiked with varying concentrations (2% and 4%) of SLO. The resultant organic fertilizers were employed as amendment in pollution simulated potted soils laid out in a complete randomized block design with three replications for 90 days. Results revealed higher counts of hydrocarbon utilizing microbes (HUB: 4.2±0.02×104cfu/g in Ft2, 3.0±0.02×104cfu/g in Ft4; HUF: 3.9±0.2×104cfu/g in Ft2, 2.5±0.02×104cfu/g in Ft4) in spiked compost compared to the control, Ft0 (HUB: 7.9±0.02×103cfu/g; HUF: 6.0±0.2×103cfu/g). Mean count in amended soils reflected a dose-dependent increase which followed the trend: Ft2 ˃ Ft0 ˃ Ft4 for the 5% (3.7×108 cfu/g), 10% (9.2×107 cfu/g) and 15% (6.9×107 cfu/g) levels of fertilizer treatments respectively. There was a significant (P&lt;0.05) reduction in the TPH content of soils after 90days treatment with organic fertilizers. Generally, remediation efficiency followed the order: Ft2 ˃ Ft0 ˃ Ft4, with the highest (11.51%) achieved at 5% Ft2 application. Spiking technique was responsible for the higher counts of hydrocarbon utilizing microbes and enhanced bioremediation associated with the use of fertilizers Ft2.

Dose–effect of nitrogen regulation on the bioremediation of diesel contaminated soil

Nitrogen regulation is an effective method to enhance the bioremediation of hydrocarbon contamination. In this study, various dosages of two types of nitrogen sources were spiked to the diesel contaminated soil in a 60-day microcosmic experiment. The results showed that the total petroleum hydrocarbon (TPH) degradation rate improved from control test of 32.03% to the highest of 44.74% with nitrogen spiking. Peptone and KNO3 significantly improved the bioremediation of diesel-contaminated soil, peptone was more effective than KNO3 at low dosage. The soil C:N ratio of 20:1 (T1 treatment with the addition of peptone) was the optimal treatment. The effect of two nitrogen on soil pH was reverse, high dose of peptone addition significantly increased soil pH, but KNO3 addition significantly decreased soil pH. The soil bacteria diversity decreased significantly in the high dose Peptone treated soil, while the changes of bacteria diversity of KNO3 treated soil was just opposite. Furthermore, nitrogen regulation significantly changed the structure of soil bacterial community, Rubrobacter, Solirubrobacter and Gaiella, which belonging to Actinomycetota, were identified as the three common genus with hydrocarbon degrading ability in different nitrogen amended soil. Peptone and KNO3 had different mechanisms on the bioremediation of diesel contaminated soil. The properties of these two nitrogen sources provides us with more options for the bioremediation of hydrocarbon contaminated acid or alkaline soil.

Enhanced phytoremediation of petroleum-contaminated soil by biochar and urea

Application of bioremediation in petroleum-contaminated soils is limited by its low efficiency. Although biochar and urea are commonly used soil additives, their potential beneficial effect on the bioremediation of petroleum contamination have rarely been discussed. In this study, biochar and urea were combined to test their effects on the phytoremediation of petroleum-contaminated soil in pot experiments. Our results showed that the degradation rate of total petroleum hydrocarbons reached 49.6%, 38.3%, 42.5%, and 77.9% when the soil was treated with biochar, urea, ryegrass, and their integrated application treatment (PBCN), respectively. A number of soil physicochemical properties (e.g., pH, elements, aggregate distribution, and organic matter composition) altered by the treatments were found to be linked to the accelerated degradation of petroleum hydrocarbons. The activities of soil dehydrogenase, lipase, and urease, and the abundances of 16 s rRNA gene and alkane degradation-related genes could be increased simultaneously when biochar, urea, and ryegrass were co-applied. Furthermore, urea significantly reduced soil bacterial α-diversity, while soil bacterial community dissimilation was mainly driven by urea and ryegrass. Lysobacter, xanthomonadaceae, and longimicrobia could be biomarker species in the PBCN group. Soil bacterial network analysis showed that biochar and urea application decreased the network complexity and robustness, while ryegrass behaved inversely. Lastly, soil metabolomic analysis revealed that root soil metabolites were greatly affected by urea-addition during phytoremediation, and co-application of biochar and urea could activate the putative metabolism pathway of petroleum hydrocarbons in root soil (e.g., naphthalene and anthracene degradation, and pyruvate metabolism). In summary, this study confirmed the enhancement of biochar and urea application in the phytoremediation of petroleum-contaminated soil and explored the internal mechanism of the interactive effect, which can potentially improve the development of eco-friendly and cost-effective in-situ bioremediation technology for petroleum-contaminated soils.

A sustainable approach to enhance heavy hydrocarbons removal in landfarming treatment.

The present study aimed to evaluate the best strategy to enhance the degradation rate of heavy petroleum hydrocarbons (HPH) contaminated soil in a landfarming plant. Samples of real contaminated soil, further spiked with HPH, were treated in mesocosm reactors simulating the landfarming system. One reactor was operated without any modification compared to the real landfarming plant. The other three reactors were operated with different strategies to improve the removal rate: biostimulation (BS) through the addition of nitrogen and phosphorus; bioaugmentation (BA) with the inoculation of sludge produced in the treatment of the process water from the oil re-fining plant of the same industrial area; combination of biostimulation and bioaugmentation (BAS). The biostimulation (BS) was the most effective strategy, leading to a reduction of the remediation time by 35% as compared to the traditional treatment. Bioaugmentation (BA) also provided positive effects leading to a reduction of the remediation time by 24%; its performance improved further when the addition of sludge was combined with the increase of phosphorous (BAS). Therefore, the key tool was represented by the phosphorous availability, whereas the application of sludge was most useful to provide waste with a new possibility of reuse, thus fulfilling the principles of the circular economy. The final characterization showed that the treated soil was suitable for reuse in industrial areas according to the legislation in force.

Lead or cadmium co-contamination alters benzene and toluene degrading bacterial communities

Co-contamination of hydrocarbons with heavy metals in soils often complicates and hinders bioremediation. A comprehensive characterization of site-specific degraders at contaminated sites can help determine if in situ bioremediation processes are sufficient. This study aimed to identify differences in benzene and toluene degradation rates and the microbial communities enriched under aerobic conditions when different concentrations of Cd and Pb are introduced. Microcosms were used to study the degradation of 0.23 mM benzene or 0.19 mM toluene under various concentrations of Pb (up to 240 µM) and Cd (up to 440 µM). Soil collected from a stormwater retention basin receiving runoff from a large parking lot was utilized to seed the microcosms. The hydrocarbon degradation time and rates were measured. After further rounds of amendment and degradation of benzene and toluene, 16S rRNA gene amplicon sequencing and quantitative PCR were used to ascertain the microbial communities enriched under the various concentrations of the heavy metals. The initial degradation time for toluene and benzene was 7 to 9 days and 10 to 13 days, respectively. Degradation rates were similar for each hydrocarbon despite the concentration and presence of metal co-contaminant, however, the enriched microbial communities under each condition differed. Microcosms without metal co-contaminant contained a diversity of putative benzene and toluene degrading bacteria. Cd strongly reduced the richness of the microbial communities. With higher levels of heavy metals, genera such as Ralstonia, Cupriavidus, Azoarcus, and Rhodococcus became more dominant under various conditions. The study finds that highly efficient benzene- and toluene-degrading consortia can develop under variations of heavy metal co-contamination, but the consortia are dependent on the heavy metal type and concentrations.

Predicting selected kinetic parameters of hydrocarbon interactions with alum-based water treatment residuals

Kinetic parameters of hydrocarbon interactions with alum-based water treatment residuals (WTR) in the biostimulation of crude oil-polluted soil are very important because they reveal how long the WTR bind with the hydrocarbon to control its fate and transport in the soil. In this study, we used Michaelis-Menten biokinetic model to determine two important kinetic parameters, the maximum specific rate constant (μmax) and Michaelis-Menten constant (Km), as well as total petroleum hydrocarbon (TPH) degradation rate equation in the biostimulation of crude oil-polluted Niger Delta Oxisols using WTR synthesized from local fishpond effluents. Using the completely randomized design, the experimental setup consisted of 6-duplicate reactors including the control (labelled A to F) containing mixtures of soil, crude oil, and WTR in the ratios of 4:1:0, 4:1:1, 4:1:1.5, 4:1:2, 4:1:2.5, and 4:1:3, respectively. Treatment lasted for 70 days and TPH concentration was determined by gas chromatography-mass spectrometry (GC-MS). Results showed that a rounded average of 51% TPH reduction was achieved after 70 days of treatment. The rounded average of μmax was 0.5 mg/kg-day and Km was 270 mg/kg, while the TPH degradation rate equation was deduced as r=−0.5S/(270+S) at any TPH concentration, S. With an average root-mean-square error of 4.998 mg/kg, residual prediction deviation of 18.08, and corresponding validation r2 of over 99%, the model prediction was categorized as excellent with an error of only 1%. This shows the potential of the model to predict TPH in the biostimulation of crude oil-polluted Niger Delta Oxisols with alum-based WTR.

Root exudate glycine synergistically promotes phytoremediation of petroleum-contaminated soil

The exploitation of petroleum, the production and use of petroleum products causes pollution that is harmful to the ecology and environment. At present, petroleum hydrocarbon pollution has become a universal concern in the world. As one of the bioremediation methods, plant root exudate repair has the effect of eliminating toxic substances in the environment. To explore the role of root exudates glycine in phytoremediation of petroleum-contaminated soil, seed germination and pot experiments were carried out to study the effects of glycine on the degradation of petroleum hydrocarbons in different plants and soil types. The results showed that when the concentration of petroleum pollutants was constant, the order of seed germination rate of the eight plants was ryegrass &amp;gt; sudan grass &amp;gt; white clover &amp;gt; tall fescue &amp;gt; alfalfa &amp;gt; pennisetum &amp;gt; canine root &amp;gt; maize grass.1000 mg L−1 of glycine could effectively promote plant biomass and the petroleum hydrocarbons degradation rate. The degradation effect was ryegrass + glycine &amp;gt; ryegrass &amp;gt; sudan grass + glycine &amp;gt; sudan grass &amp;gt; white clover + glycine &amp;gt; white clover. The degradation rate of ryegrass + glycine in 18,000 mg kg−1 petroleum-contaminated soil reached 55.7%. All plants had the highest biomass and plant height in loamy soil, while the highest degradation rate of petroleum hydrocarbons was observed in sandy soil, ranging from 50.36% to 59.36%. Among them, ryegrass combined with 1000 mg L−1 of glycine reached the highest petroleum hydrocarbons degradation rate of 59.36% in sandy soil. Ryegrass, sudan grass and white clover had the potential to remediate petroleum-contaminated soils. In the three types of soil, ryegrass had a strong remediation efficiency, and the degradation effect to petroleum hydrocarbons was more significant after the addition of glycine. Therefore, ryegrass can be planted with glycine as a priority for remediation in oil field areas contaminated by petroleum hydrocarbons.

Coupling biostimulation and phytoremediation for the restoration of petroleum hydrocarbon-contaminated soil

Total petroleum hydrocarbons (TPH) continue to be among the most common pollutants in soil worldwide. Bioremediation and phytoremediation have become sustainable ways of dealing with TPH contamination and biostimulation-assisted phytoremediation is considered as a potential approach for the treatment of pollutants. In this study, the response surface was used to optimize the single-factor biological stimulation experiment of moisture content, leavening agent content and compound fertilizer content and got the best experimental plan of biological stimulation. It was found that TPH degradation rate was 28.6% by biostimulation after 70 days. Further, from 20 kinds of plant seeds, 5 kinds of suitable or growth and high germination rate were selected for petroleum hydrocarbon degradation experiment. In the phytoremediation, peanut was selected as the best plant species by measuring the TPH degradation rate, bacteria count, growth of test plants, germination rate and amount of catalase in the soil and it could achieved 31.1% degradation rate of petroleum hydrocarbons after 70 days. Finally, the artificial biostimulation and phytoremediation combined degradation experiment of petroleum hydrocarbons-contaminated soil was designed and it achieved 38.9% TPH degradation rate after 70 days.

Comparative Analysis of Microbial Consortiums and Nanoparticles for Rehabilitating Petroleum Waste Contaminated Soils.

Nano-bioremediation application is an ecologically and environmentally friendly technique to overcome the catastrophic situation in soil because of petroleum waste contamination. We evaluated the efficiency of oil-degrading bacterial consortium and silver nanoparticles (AgNPs) with or without fertilizer to remediate soils collected from petroleum waste contaminated oil fields. Physicochemical characteristics of control soil and petroleum contaminated soils were assessed. Four oil-degrading strains, namely Bacillus pumilus (KY010576), Exiguobacteriaum aurantiacum (KY010578), Lysinibacillus fusiformis (KY010586), and Pseudomonas putida (KX580766), were selected based on their in vitrohydrocarbon-degrading efficiency. In a lab experiment, contaminated soils were treated alone and with combined amendments of the bacterial consortium, AgNPs, and fertilizers (ammonium nitrate and diammonium phosphate). We detected the degradation rate of total petroleum hydrocarbons (TPHs) of the soil samples with GC-FID at different intervals of the incubation period (0, 5, 20, 60, 240 days). The bacterial population (CFU/g) was also monitored during the entire period of incubation. The results showed that 70% more TPH was degraded with a consortium with their sole application in 20 days of incubation. There was a positive correlation between TPH degradation and the 100-fold increase in bacterial population in contaminated soils. This study revealed that bacterial consortiums alone showed the maximum increase in the degradation of TPHs at 20 days. The application of nanoparticles and fertilizer has non-significant effects on the consortium degradation potential. Moreover, fertilizer alone or in combination with AgNPs and consortium slows the rate of degradation of TPHs over a short period. Still, it subsequently accelerates the rate of degradation of TPHs, and a negligible amount remains at the end of the incubation period.

Microbial community analysis of biopiles in Antarctica provides evidence of successful hydrocarbon biodegradation and initial soil ecosystem recovery

Microorganisms comprise the bulk of biodiversity and biomass in Antarctic terrestrial ecosystems. To effectively protect and manage the Antarctic environment from anthropogenic impacts including contamination, the response and recovery of microbial communities should be included in soil remediation efficacy and environmental risk assessments. This is the first investigation into the microbial dynamics associated with large scale bioremediation of hydrocarbon contaminated soil in Antarctica. Over five years of active management, two significant shifts in the microbial community were observed. The initial shift at 12–24 months was significantly correlated with the highest hydrocarbon degradation rates, increased microbial loads, and significant increases in alkB gene abundances. ANCOM analysis identified bacterial genera most likely responsible for the bulk of degradation including Alkanindiges, Arthrobacter, Dietzia and Rhodococcus. The second microbial community shift occurring from 36 to 60 months was associated with further reductions in hydrocarbons and a recovery of amoA nitrification genes, but also increasing pH, accumulation of nitrite and a reduction of oligotrophic bacterial species. Over time, the addition of inorganic fertilisers altered the soil chemistry and led to a disruption of the nitrogen cycle, most likely decoupling ammonia oxidisers from nitrite oxidisers, resulting in nitrite accumulation. The results from this study provide key insights to the long-term management of hydrocarbon bioremediation in Antarctic soils.

Anaerobic Corrosion of Zero-Valent Iron at Elevated Temperatures.

Increasing groundwater temperatures caused by global warming, subsurface infrastructure, or heat storage projects may interfere with groundwater remediation techniques using zero-valent iron (ZVI) technology by accelerating anaerobic corrosion. The corrosion behavior of three ZVIs widely used in permeable reactive barriers (PRBs), Peerless cast iron (PL), Gotthart-Maier cast iron (GM), and an ISPAT iron sponge (IS), was investigated at temperatures between 25 and 70 °C in half-open batch reactors by measuring the volume of hydrogen gas generated. Initially, the corrosion rates of all tested ZVIs increased with temperature; at temperatures ≤40 °C, a material-specific steady state is reached, and at temperatures >40 °C, passivation causes a decrease in long-term corrosion rates. The observed corrosion behavior was therefore assumed to be superimposed by accelerating and inhibiting effects, caused by surface precipitates where the fitting of measured corrosion rates by a modeling approach, using the corroded amount of Fe0 to account for passivating minerals, yields intrinsic activation energies (Ea, ZVI) of 81, 90, and 107 kJ mol-1 for IS, GM, and PL, respectively. An increase in H2 production might not be directly transferable to an increase in general ZVI reactivity; however, the results suggest that an increase in chlorinated hydrocarbon degradation rates can be expected for ZVI-PRBs in the immediate vicinity of low-temperature underground thermal energy storages (UTESs) or in the impact areas of high-temperature UTES with temperatures of ≤40 °C.

Plant-Soil-Microbiota Combination for the Removal of Total Petroleum Hydrocarbons (TPH): An In-Field Experiment.

The contamination of soil with total petroleum hydrocarbons (TPH) may result in dramatic consequences and needs great attention, as soil rehabilitation would need more effort from a sustainability perspective. However, there is still no known general method since the remediation technology is strictly site-specific. Adaptive biological system dynamics can play a key role in understanding and addressing the potential of situ-specific biological combinations for soil pollutants removal. The potential worst-case of TPH contamination reflects soil affected by heavy industrial activities, such as oil refineries. Therefore, the experimental trial was conducted on a 2,000 m2 area from a contaminated site located in northern Italy. We evaluated the remediation potential over time (270 days) assessing (i) the phytoremediation efficiency of two species of Poaceae (Festuca arundinacea Schreb. and Dactylis glomerata L.) and two species of Fabaceae (Medicago sativa L. and Lotus corniculatus L.) and (ii) the role of the indigenous bacteria flora and endo-mycorrhizae consortium addition in plant growth promotion. We also induced resistance to contamination stress in a field experiment. Thirty-three indigenous bacteria selected from the contaminated soils showed marked plant growth promotion. Moreover, functional metagenomics confirmed the metabolic capability of hydrocarbon-degrading microorganisms living in the polluted soil. Our data showed that soil enzymatic activities increased with hydrocarbon degradation rate after 60 days. Both Poaceae and Fabaceae resulted in remarkable remediation potential. Stress markers and antioxidant activity indicated that the selected plant species generally need some time to adapt to TPH stress. In conclusion, our evaluation implied both the rhizosphere effects and functional features of the plant and suggested that plants should (i) have marked tolerance to specific contaminants, (ii) be characterized by an extensive root system, and (iii) be susceptible to arbuscular mycorrhizal fungi (AMF) infection.

Remarkable shift in structural and functional properties of an animal charcoal-polluted soil accentuated by inorganic nutrient amendment

BackgroundSoils polluted with animal charcoal from skin and hide cottage industries harbour extremely toxic and carcinogenic hydrocarbon pollutants and thus require a bio-based eco-friendly strategy for their depuration. The effects of carbon-free mineral medium (CFMM) amendment on hydrocarbon degradation and microbial community structure and function in an animal charcoal-polluted soil was monitored for 6 weeks in field moist microcosms consisting of CFMM-treated soil (FN4) and an untreated control (FN1). Hydrocarbon degradation was monitored using gas chromatography-flame ionization detector (GC-FID), and changes in microbial community structure were monitored using Kraken, while functional annotation of putative open reading frames (ORFs) was done using KEGG KofamKOALA and NCBI’s conserved domain database (CDD). ResultsGas chromatographic analysis of hydrocarbon fractions revealed the removal of 84.02% and 82.38% aliphatic and 70.09% and 70.14% aromatic fractions in FN4 and FN1 microcosms in 42 days. Shotgun metagenomic analysis of the two metagenomes revealed a remarkable shift in the microbial community structure. In the FN4 metagenome, 92.97% of the population belong to the phylum Firmicutes and its dominant representative genera Anoxybacillus (64.58%), Bacillus (21.47%) and Solibacillus (2.39%). In untreated FN1 metagenome, the phyla Proteobacteria (56.12%), Actinobacteria (23.79%) and Firmicutes (11.20%), and the genera Xanthobacter (9.73%), Rhizobium (7.49%) and Corynebacterium (7.35%), were preponderant. Functional annotation of putative ORFs from the two metagenomes revealed the detection of degradation genes for aromatic hydrocarbons, benzoate, xylene, chlorocyclohexane/chlorobenzene, toluene and several others in FN1 metagenome. In the FN4 metagenome, only seven hydrocarbon degradation genes were detected. ConclusionThis study revealed that though CFMM amendment slightly increases the rate of hydrocarbon degradation, it negatively impacts the structural and functional properties of the animal charcoal-polluted soil. It also revealed that intrinsic bioremediation of the polluted soil could be enhanced via addition of water and aeration.

Remediation potential of immobilized bacterial strain with biochar as carrier in petroleum hydrocarbon and Ni co-contaminated soil

ABSTRACT The remediation of organic pollutant-heavy metal co-contaminated soil is a great challenge. Immobilized microorganism technology (IMT) is a potential approach to remediate co-contaminated soil. In this study, we evaluated the feasibility of IMT for the remediation of petroleum hydrocarbon-heavy metal nickel (Ni) co-contaminated soil. The Ni resistant and hydrocarbon-degrading bacteria strain Citrobacter sp. was added to co-contaminated soil by immobilizing on corncob biochar. The potential performance in biodegradation of petroleum hydrocarbon and changing the mobility and speciation of nickel (Ni) in soil were determined, with consideration of the influences of the soil properties and dehydrogenase activity. The results demonstrated that the degradation rate of petroleum hydrocarbons by immobilized microorganisms group (IM) was 45.52%, significantly higher than that of the free bacteria (30.15%), biochar (25.92%) and blank group (18.47%) (P<0.05). At the same time, IM was more effective in immobilizing Ni in the soil by transforming available Ni to a stable fraction with a maximum residual concentration increasing by 101.50 mg·kg−1, and the carcinogenic nickel sulfide was not detected after remediation in IM. IM exhibited a higher level of soil dehydrogenase activity (0.3956 μg·mL−1·h−1·g−1) than that of free bacteria (0.2878 μg·mL−1·h−1·g−1). A linear correlation was found between the petroleum pollutants degradation rate and dehydrogenase activity (P<0.05). This study indicates the effectiveness and potential of IMT application in degrading petroleum hydrocarbon and immobilizing heavy metals in co-contaminated soil.