Addressing the critical need for precise streamflow measurements in hydro-environmental research, this study evaluates large-scale particle image velocimetry (LSPIV) using cost-effective closed-circuit television (CCTV) cameras, providing a detailed sensitivity analysis in both laboratory and real-world canal settings. In laboratory conditions, a 45° camera angle notably enhanced performance, exhibiting a 12% decrease in MAE and a remarkable 40% reduction in RMSE compared to the performance of orthographic form. Tracer particles further enhanced LSPIV accuracy, decreasing both mean absolute error (MAE) and root mean square error (RMSE) by around 0.05 m/s. Optimal velocity coefficients for the lab ranged between 0.85 and 0.90. Nighttime measurements, using projection-based illumination, showed a minor 3% MAE variation and 0.02 RMSE difference versus daytime. In field experiments, a 45° upstream CCTV camera configuration notably improved LSPIV accuracy, achieving a 3% MAE and 0.055 m/s RMSE. For best results across different turbidity levels, we recommend a velocity coefficient range of 0.84 to 0.88. This study highlights the robustness and cost-efficiency of LSPIV as a transformative method for streamflow gauging, demonstrating its wide applicability in diverse hydro-environmental scenarios.