Hydrazyl Free Radical Scavenging Activity Research Articles

PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF PTEROCARPUS SANTALINUS L.F. PLANT PARTS

Objective: Oxidation is a normal process in many biological metabolisms in living beings, but in this technology era radiation, pollution, irregular life style, and unhealthy diet leads to excessive oxidation resulting in imbalance between antioxidants and free radicals in body called oxidative stress, which may result in cardiac problems, diabetes, and carcinogenic diseases. Therefore, exploration of natural antioxidants in flora and fauna is becoming important. In this motive a phytochemical study was taken up on various parts of Pterocarpus santalinus L.f. plant belonging to Leguminosae family to check the presence of antioxidant activity (AA).
 Methods: The leaves, flowers, fruits, and roots of this plant were extracted with six different organic solvents from polar to non-polar. To estimate the AA of these extracts, four types of antioxidant tests were done on them, namely, α, α – Diphenyl – β – Picryl - Hydrazyl free radical scavenging activity (DPPH RSA) assay, relative reducing power (RRP) assay, Ferric thiocyanate (FTC) assay, and β - carotene bleaching assay with Butylated Hydroxy Toluene (BHT) as synthetic standard.
 Results: In extraction, Me extracts showed highest yields. Qualitative phytochemical screening has revealed many bioactive components in the Me and Et extracts of plant parts majorly. In DPPH RSA assay, Me extracts of leaves showed RSA activity almost equal to reference standard. In RRP assay, Me floral extracts showed AA higher than standard. FTC and β – Carotene assays showed that Me sample extracts possess good AA than BHT. In FTC assay, lower absorption values specify high AA, whereas in β – Carotene assay, high absorption values indicate high AA of the sample extracts.
 Conclusion: Among six solvents methanol showed highest extraction efficiency and also revealed highest number of phytochemicals in its extracts. These four AA assays revealed that the methanol extracts of all plant parts, that is., leaves, roots, fruits, and flowers possess better AA compared other solvent extracts.

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana).

Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of ZnO and CuO ENPs on in vitro roots formation, non-enzymatic antioxidant activities, and production of steviol glycosides (SGs) in regenerants of Candyleaf, Stevia rebaudiana. ENPs were applied in 0, 2, 20, 200, and 2000 mg/L of concentration in the MS medium containing plant shoots. The percentage of rooting induced was 91% and 94% by applying ZnO ENPs (2 mg/L) and CuO ENPs (20 mg/L), respectively. Moreover, at 2 mg/L of ZnO and 20 mg/L of CuO ENPs, the high performance liquid chromatography studies determined the significantly greatest content of SGs; rebaudioside A (4.42 and 4.44) and stevioside (1.28 and 1.96). Phytochemical studies including total flavonoid content, total phenolic content, and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated highest by the regenerants grown in 2 mg/L of ZnO and 20 mg/L of CuO ENPs dosage. Both ZnO and CuO ENPs at 200 mg/L and 2000 mg/L of concentration induced adverse effects on plant biomass, antioxidant activities, and SGs content up to 1.22 and 1.77 for rebaudioside A and 0.21 and 0.25 for stevioside. Hence, the biochemical and morphophysiological responses of Candyleaf were elicited as a defense against ZnO and CuO ENPs applied under threshold limit. This artificial biotechnological technique holds great promise for continued production of natural antioxidants on commercial scale and our study has further strengthened this impact.

In-vitro Re-evaluation of Antioxidant activity by 2, 2-Diphenyl-1picrylhydrazyl Free Radical (DPPH) Assay in Medicinal Plants of Andhra Pradesh, India.

Medicinal plants are a major source of raw materials on the globe from ancient to present century for the traditional system like Ayurveda, Siddha and Unani. Even the modern system of medicine has more than 25 percent of drugs in use which are either plant based or plant products. In day to day life, particularly in India people are suffering from menacing health diseases from common cold to amnesia and poisonous snake bites. These diseases can be treated by using some herbal extracts from plants which has antioxidant activity. Medicinal plants like Azadirachta indica, Ocimum sanctum, Lawsonia intermis, Murraya koenigii, Curcuma longa and Cuminum cymium belonging to different families play a vital role in day to day usage of different indigenous communities due to its sacred and medicinal value. Recently there has been an upsurge of interest in the therapeutic potential of medicinal plants as antioxidants. In the course of finding potential antioxidant from plant source, six medicinal tree species belonging to different families has been selected. Leaves were dried and extracted with methanol solvent systems. Antioxidant activity using 1, 1-diphenyl-2-picryl hydrazyl radical scavenging assay, of six extracts from six genus of different families are reported and a comparison of the free radical scavenging ability of the extracts is emphasized. The highest percentage of 1,1-diphenyl-2-picryl hydrazyl free radical scavenging activity is found in Azadirachta indica (81.8% of inhibition) which shows antimicrobial, anti inflammatory and anticancerous properties where Ascorbic acid (68.5 µg/ml) is taken as standard for comparison.

Abiotic stress of ZnO‐PEG, ZnO‐PVP, CuO‐PEG and CuO‐PVP nanoparticles enhance growth, sweetener compounds and antioxidant activities in shoots of Stevia rebaudiana Bertoni

Nanoparticles are known to play remarkable role as abiotic stress elicitors in plants. This study reports the comparative analysis of effects produced by capped [zinc oxide (ZnO)-polyethylene glycol (PEG), ZnO-polyvinyl pyrrolidone (PVP), copper oxide (CuO)-PEG, CuO-PVP] and uncapped (ZnO and CuO) nanoparticles on the medicinal plant, Stevia rebaudiana raised in vitro for the production of commercially important sweetener compounds. In context of shoot organogenesis, ZnO-PEG, ZnO-PVP, CuO-PEG, CuO-PVP were employed to the growth medium that resulted in increased growth parameters, and larger content of steviol glycosides as compared to the shoots raised in medium containing ZnO and CuO, revealed by high-performance liquid chromatography. In the meanwhile, non-enzymatic antioxidant activities including total phenolic content, total flavonoid content, total antioxidant capacity, total reducing power and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated and showed comparatively greater amounts in shoots grown in medium containing capped ZnO or CuO nanoparticles. Furthermore, the ZnO and its derivatives revealed to be more reactive at 1 mg/l of concentration. Whereas, the CuO and its derivatives produced greater response on Stevia at 10 mg/l concentration of nanoparticles. This study paves the way for more such studies encompassing capped and uncapped nanoparticles and their ultimate effect on in-vitro grown plant tissues for the production of active metabolites on industrial scale.

Differential anticancer effect of fermented squid jeotgal due to varying concentrations of soymilk additive

Fermentation plays a vital role in the nutritional enrichment of food. Korea has a long tradition of adding fermented food to the daily diet and jeotgal is one of the common fermented and salted foods in Korean cuisine. In our study, we added soymilk as an additive to squid jeotgal to improve its functionality. We mixed different concentrations of soymilk (2, 5, and 10 mg/g) with squid jeotgal samples, fermented them for one week, and then tested their antioxidant and anticancer activities to compare with those of squid jeotgal samples without soymilk additive. To investigate the anticancer characteristics, glutathione-S-transferase (GST)-pi enzyme assay was used. To test the antioxidant activities, various assays were performed, including 2,2-diphenyl-1-picryl hydrazyl free radical scavenging activity, 2,2-azino-bis(3-ethyl-benzothiazoline-6-sulfonic acid) diammonium saltradical cation scavenging assay, and reducing power assay. Samples fermented with a small amount of soymilk showed excellent anticancer activity. The addition of only 2 mg/g of soymilk to squid jeotgal inhibited the activity of GST-pi by almost 50% when compared with the sample with no addition. Moreover, no undesirable bitterness or astringency was noticed. Our results could help to improve the current food status of squid jeotgal and it could be used to reduce the risk of chronic disease along with its basic nutritional function.

Stabilization of winterized cottonseed oil with chia (Salvia hispanica l.) seed extract at ambient temperature

Antioxidant activity of ethanolic chia seed extract for the stabilization of winterized cottonseed oil was investigated. Refined, bleached, winterized and deodorized cottonseed oil was supplemented with ethanolic chia seed extract at 250, 500 and 750 ppm concentrations (T1, T2 and T3, respectively). Cottonseed oil without any addition of chia seed extract served as control. Cottonseed oil added with 200 ppm butylated hydroxyl anisole was used as positive control. Experimental samples and control were packaged in transparent, 250 mL polyethylene terephthalate bottles, stored at ambient temperature (30–32 °C) for 90 days, sampled at 0, 45 and 90 days for the assessment of storage stability. Diphenyl picryl hydrazyl free radical scavenging activity of ethanolic chia seed extract and butylated hydroxyl anisole was 85.3 and 87.1 %. The concentration of caffeic acid, quercetin, phenolic glycoside k, glycoside Q and Kaempferol in ethanolic chia seed extract was 0.22, 0.015, 0.07, 0.19 and 0.45 mg/mL. Fatty acid composition of 90 days stored T3 and 200 ppm butylated hydroxyl anisole samples was not different from each other (p > 0.05). Induction period of control, T3 and 200 ppm butlated hydroxyl supplemented cottonseed oil was 3.57, 8.45 and 8.62 h. After 90 days of storage period, peroxide value of control, T3 and 200 ppm butylated hydroxyl anisole supplemented samples was 2.75, 1.16 and 1.05 (meqO2/kg). The results of this study evidenced that ethanolic chia seed extract can be used for the long term preservation of winterized cottonseed oil at ambient temperature.

Online antioxidant activity and ultra-performance LC-electrospray ionisation-quadrupole time-of-fight mass spectrometry for chemical fingerprinting of Indian polyherbal formulations

A HPLC–DAD–DPPH method was developed for evaluating the 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging activity of ethylacetate extracts of different polyherbal formulations (draksarista, draksava, lohasava and arvindasava) by using RP-18e column. The ethylacetate extract from polyherbal, ‘draksarista’ exhibited maximum free radical scavenging activity (99.9 ± 0.38%) followed by draksava (99.8 ± 0.34%), lohasava (98.5 ± 0.30%) and arvindasava (42.3 ± 0.34%) at 100 μg mL− 1. Simultaneously, ultra-performance liquid chromatography coupled with electrospray ionisation-quadrupole time-of-flight mass spectrometry (UPLC-ESI-QTOF-MS) was used to study chemical composition of the ethylacetate extracts of formulations. The characteristic electrospray mass ionisation reveals the dominance of polyphenols and their glycosides in the four polyherbal formulations.

Anti-inflammatory and anti-nociceptive activities of extracts and compounds isolated from Solenostemmaarghel (Del.) Hayne

AIM: Validation of the anti-inflammatory activity of Solenostemmaarghel (Del.) Hayne, which isused in folk medicine. Settings and Design: Total alcohol extract, successive extracts (ether, chloroform, ethyl acetate and butanol) and isolated compounds of S. arghel were evaluated at different doses for their anti-inflammatory, antinociceptive, anti-pyretic and anti-oxidant activities. MATERIALS AND METHODS: Carrageenin-induced rat paw oedema, yeast-induced hyperthermia in rats, writhing test and hot plate test techniques in mice and in vitro Di-phenyl picryl hydrazyl free radical scavenging activity test were used. Identification of the isolated compounds were established by m.p., UV, 600 MHz 1H and 13C NMR. STATISTICAL ANALYSIS: Analysis of variances was carried out in two ways using ANOVA test followed by Tukey HSD test using SPSS, version 14. RESULTS: Total alcohol extract (250, 500 and 1000 mg/kg), successive extracts (250 mg/kg) and isolated compounds (100 mg/kg) showed different potentials of the reported pharmacological activities, where the ethyl acetate successive extract showed the highest activity. Two flavonoides were isolated from the ethyl acetate extract and identified as C1 (kaempferol-3, 4'-diglucoside) and C2 (kaempferol 3-rutinoside). Total alcohol extract (1000 mg/kg) administrated daily to rats for 14 consecutive days did not show any alterations on liver and kidney functions and on the oxidative balance. CONCLUSIONS: Results revealed that C1 and C2possessed potent anti-inflammatory and anti-nociceptive activities and they seem to be the active constituents of the investigated extract. The reported anti-inflammatory and analgesic properties and the anti-oxidant potential of C1 and C2 could be due to their flavonoid structure.

Aqueous extract of Boerhaavia diffusa root ameliorates ethylene glycol-induced hyperoxaluric oxidative stress and renal injury in rat kidney

Introduction: Boerhaavia diffusa Linn. (Nyctaginaceae) is widely used in traditional Indian medicines against renal afflictions including calcium oxalate (CaOx) urolithiasis and is known for antioxidant activity.Objective: The present study was designed to investigate the ameliorating effect of aqueous extract of B. diffusa roots (BDE) in hyperoxaluric oxidative stress and renal cell injury.Material and methods: In vitro antioxidant activity of BDE was estimated in terms of total phenolic content and 1,1-diphenyl-2-picryl hydrazyl free radical scavenging activity. Wistar albino rats were given 0.75% v/v ethylene glycol in drinking water to induce chronic hyperoxaluria and simultaneously BDE was given to nephrolithiasic treated rats at the dose of 100 and 200 mg/kg b.w. orally for 28 days. Urinary volume, oxalate, serum creatinine, blood urea nitrogen (BUN), malondialdehyde (MDA) and antioxidant enzyme (SOD, CAT, GST, GPx) were evaluated.Results and discussion: BDE extract was found to posses a high total phenolic content and exhibited significant free radicals scavenging activity. Oxalate excretion significantly increased in hyperoxaluric animals as compared to control which was protected in BDE-treated animals. BDE treatment significantly reduced level of MDA and improved the activity of antioxidant enzymes followed by reduction in BUN and serum creatinine. In addition, BDE reduced the number of CaOx monohydrate crystals in the urine. Histological analysis depicted that BDE treatment inhibited deposition of CaOx crystal and renal cell damage.Conclusion: The present study reveals that antioxidant activity of BDE significantly protects against hyperoxaluric oxidative stress and renal cell injury in urolithiasis.

Antidiabetic and Free Radicals Scavenging Potential of Euphorbia hirta Flower Extract.

The present study was carried out to evaluate antidiabetic and in vitro free radicals scavenging effects of flower extract of Euphorbia hirta. The ethanolic and petroleum ether extracts (250 and 500 mg/kg) were orally tested for 21 days in alloxan induced diabetic mice and blood glucose level was measured with glucometer. Administration of extract resulted in significant reduction in serum cholesterol, triglycerides, creatinine, urea, alkaline phosphatase levels but high density lipoprotein levels and total proteins were found to be increased after treatments. Free radicals scavenging effect of ethanolic extract was also evaluated by various antioxidant assays, including 1, 1-diphenyl-2-picryl hydrazyl free radical scavenging activity, superoxide anion radical scavenging, nitric oxide scavenging, and reducing power assay. It was compared with standard antioxidants compounds such as butylated hydroxyl anisole and ascorbic acid. All the extracts showed antioxidant activity in all the tested methods.