Soil hydraulic properties are essential for understanding soil hydrological and erosion processes. The importance of roots on soil hydraulic parameters has been widely recognized, but current studies have focused mainly on the root effect of a single plant species. However, few studies have been carried out to investigate the effect of mixed plant roots on soil hydraulic parameters. Undisturbed soil samples were collected from three natural sampling sites with single plant types and four sites with mixed plant types. Both soil hydraulic parameters were measured: soil saturated hydraulic conductivity (Ks) and soil saturated water content (SW). Then the root effects on Ks and SW were subsequently evaluated. The results showed that the Ks and SW at the sites with mixed plants were higher than those at the sites with a single plant species. At all the sites, Ks and SW both exhibited a decreasing-increasing-decreasing trend with increasing root density. The critical root density for the above change was quantified. When fibrous roots were mixed with tap roots, Ks was mainly affected by tap roots, while SW was mainly affected by fibrous roots. The influence of roots on Ks and SW was mainly achieved by affecting soil organic matter, not by directly affecting Ks and SW. Based on the root density, the herbaceous coverage with optimal saturated hydraulic conductivity and water content was estimated to be 66.71%.