Skin Hydration Research Articles

Poly-γ-Glutamic Acid from a Novel Bacillus subtilis Strain: Strengthening the Skin Barrier and Improving Moisture Retention in Keratinocytes and a Reconstructed Skin Model

A novel Bacillus subtilis HB-31 strain was isolated from Gotjawal Wetland in Jeju Island, Republic of Korea. A mucus substance produced by this strain was identified as high-molecular-weight poly-γ-glutamic acid (γ-PGA) using NMR, Fourier transform infrared spectroscopy, and size-exclusion chromatography/multi-angle light scattering analyses. We evaluated whether γ-PGA strengthened the skin barrier using keratinocytes and a reconstructed skin model. In keratinocytes, γ-PGA treatment dose-dependently increased the mRNA expression of skin barrier markers, including filaggrin, involucrin, loricrin, serine palmitoyl transferase, fatty acid synthase, and 3-hydroxy-3-methylglutaryl coenzyme A reductase. γ-PGA also enhanced hyaluronic acid synthesis by upregulating hyaluronic acid synthase-1, -2, and -3 mRNA levels and promoted aquaporin 3 expression, which is involved in skin hydration. In the reconstructed skin model, topical application of 1% γ-PGA elevated filaggrin, involucrin, CD44, and aquaporin 3 expression, compared to the control. These results suggest that the newly isolated HB-31 can be used as a commercial production system of high-molecular-weight γ-PGA, which can serve as an effective ingredient for strengthening the skin barrier and improving moisture retention. Further research is needed to explore the long-term effects of γ-PGA on skin health and its application in treating skin conditions.

Optical Based Methods for Water Monitoring in Biological Tissue.

Skin homeostasis is strongly dependent on its hydration levels, making skin water content measurement vital across various fields, including medicine, cosmetology, and sports science. Noninvasive diagnostic techniques are particularly relevant for clinical applications due to their minimal risk of side effects. A range of optical methods have been developed for this purpose, each with unique physical principles, advantages, and limitations. This review provides an in-depth examination of optical techniques such as diffuse reflectance spectroscopy, optoacoustic spectroscopy, optoacoustic tomography, hyperspectral imaging, and Raman spectroscopy. We explore their efficacy in noninvasive monitoring of skin hydration and edema, which is characterized by an increase in interstitial fluid. By comparing the parameters, sensitivity, and clinical applications of these techniques, this review offers a comprehensive understanding of their potential to enhance diagnostic precision and improve patient care.

An International Survey on the Use of a Polyrevitalizing Solution With or Without Other Aesthetic Procedures in the Daily Practice of Aesthetic Physicians.

NCTF135HA, a versatile polyrevitalizing solution, is a potent agent for enhancing skin quality, radiance, moisture, vitality, and diminishing fine wrinkles caused by aging factors. Data demonstrate a divergence in its application from skin quality enhancement to treatment of vitiligo lesions. To know more precisely about the protocol of use among providers, alone or in combination with other procedures, we performed an international survey. A Steering Committee of dermatologists, aesthetic surgeons, and physicians developed a 32-question questionnaire based on a literature review. Hosted online from January to March 2024, it targeted healthcare professionals experienced in polyrevitalization. Responses were analyzed anonymously and reported descriptively. Practitioners adopt a balanced approach: half of their patients receiving classic Polyrevitalization (NCTF135HA alone) and the other half undergoing combination therapy (NCTF135HA with another aesthetic procedure). Most administer NCTF135HA across multiple sessions, typically three (39.7%). In combination therapy, 55.5% of practitioners use NCTF135HA for medical purposes, targeting Melasma (53.1%) and Rosacea (45.0%) for instance. Aesthetic use is prevalent, with 94.2% employing NCTF135HA for skin wrinkles (79.9%), and refreshment, rejuvenation, and hydration (73.4%). Combining NCTF135HA with hyaluronic acid (71.2%) and botulinum toxin (49.1%) is common, alongside microneedling (50.2%), peelings (32.4%), and fractional radiofrequency (25.6%). Our survey showed a homogenous distribution of NCTF135HA utilization, indicating a consensus on its application across diverse demographics. This consistency highlights its widespread acknowledgment and efficacy in various aesthetic and clinical settings. Limitations include a large respondent group from Mexico and many respondents with less than 10 years of experience.

Influence of Fluid Intake on Biophysical Properties of the Stratum Corneum in Elderly Individuals: A Cross-Sectional Study

Objective: Water plays a crucial role in human health. Inadequate water intake can lead to various health issues, particularly dehydration in the elderly. The skin, constituting approximately 30.0% of the body’s surface area, is essential in preventing dehydration, and water contributes significantly to its resilience, elasticity, and durability. This study aimed to establish a definitive relationship between fluid intake and various biophysical properties of the stratum corneum in elderly Individuals. Methods: This cross-sectional study was conducted on individuals aged 65–80 years at the Elderly Social Institution Tresna Budi Mulia 3 in Jakarta, between April and August 2023. Correlation analysis (Pearson and Spearman tests) was used to evaluate relationships between variables, and non-parametric tests (Mann–Whitney and Friedman tests) were used to assess differences. Fluid intake was assessed over 7 days. On the 8th day, the hydration status of the stratum corneum was evaluated by examining their urine concentration, skin dryness through subjective symptoms, skin water loss based on the rate of water evaporation from the skin, and skin capacitance (SCap) according to the skin’s ability to retain moisture. Results: The study involved 67 subjects with a median age of 70 years. Multiple regression analysis showed that fluid intake significantly predicted the specified symptom sum score (SRRC) (coefficient = −9.15 × 10⁻5, P = 0.001), urine specific gravity (coefficient = −0.01, P = 0.016), and SCap (coefficient = 0.005, P = 0.022). Additionally, a statistically significant weak negative correlation was found between fluid intake and hydration status (P < 0.0001) and between fluid intake and SRRC (P < 0.0001). However, no significant correlation was observed between fluid intake and transepidermal water loss (P = 0.613) or SCap (P = 0.060). Conclusion: This study highlights the potential role of fluid intake in influencing hydration status among elderly individuals. While fluid intake is significantly associated with certain biophysical properties of the skin, it does not show a consistent correlation with all measures of skin hydration and moisture retention.

Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo.

Background:Zanthoxylum piperitum (L.) DC., commonly known as Japanese pepper, is a deciduous shrub native to East Asia. Its berries are widely used as a spice, known for imparting a distinctive, tingly numbing sensation. Biologically, Z. piperitum has antimicrobial, antioxidant, and anti-inflammatory properties, and it is studied for its potential benefits in pain relief and digestive health. This study proposed a novel biotechnological Z. piperitum phytocomplex (ZPP) obtained by plant cell culture for skin health, specifically targeting collagen synthesis, extracellular matrix stability, and resilience against cellular stress. Given the bioactivity of Z. piperitum, we aimed to analyze its efficacy as a sustainable alternative for skin-supportive applications in cosmetics and supplements. Methods: ZPP was produced through stable plant cell cultures, yielding a lignan-rich (3.02% w/w) phytocomplex. Human fibroblasts (HFFs) were treated with varying ZPP concentrations to assess cellular viability, collagen metabolism, and ECM-related enzyme activities, both under normal and cell stress conditions. The in vivo assessment was performed by measuring biophysical skin parameters such as hydration, elasticity, and roughness in female volunteers for a period of six weeks. Results: In vitro, ZPP exhibited non-cytotoxicity at all concentrations tested. Under hyperosmotic stress, ZPP reduced cellular damage, suggesting enhanced resilience. ZPP upregulated lysyl oxidase (LOX) protein levels, critical for collagen cross-linking and ECM stability, with protective effects observed under oxidative/inflammatory conditions. Additionally, ZPP selectively inhibited collagenase, attenuating collagen breakdown, though antioxidant activity was modest. In vivo evaluation highlighted improved skin hydration, elasticity, and roughness. Conclusions: ZPP shows promise as a biotechnological agent for skin health, particularly in supporting collagen integrity, ECM stabilization, and cellular resilience under stress. While further studies are needed to explore its full efficacy, especially for aging and environmentally stressed skin, these findings highlight ZPP's potential as a new ingredient for cosmetic formulations aimed at skin care and the treatment of alterations caused by aging or environmental conditions.

Investigating the antioxidant and anti-inflammatory potential of Nypa fruticans: a multifaceted approach to skin protection and aging

Reactive oxygen species (ROS) produced in the mitochondria of skin cells play a significant role in the degradation of the extracellular matrix and induction of inflammatory responses, both of which are major contributors to skin aging. Antioxidants that reduce ROS production and inhibit inflammatory skin lesions are considered beneficial for the treatment of inflammatory skin diseases and prevention of skin aging. In this study, we evaluated the potential of Nypa fruticans (NF), which is known for its antioxidant properties, to mitigate tumor necrosis factor-alpha (TNF-α)- and interferon-gamma (IFN-γ)-induced damage in normal human epidermal keratinocytes. The major active constituents identified in NF include protocatechuic acid, hydroxybenzoic acid, procyanidin B, catechin, and epicatechin. NF significantly suppressed the production of ROS, nitric oxide (NO), and prostaglandin E2 (PGE2), while also reducing the levels of inflammatory cytokines interleukin-6 (IL-6) and interleukin-1 beta (IL-1β), which were elevated by TNF-α/IFN-γ stimulation. Furthermore, NF restored the expression of key skin barrier-related proteins such as serine peptidase inhibitor kazal type 5 (SPINK5), collagen type I alpha 1 chain (COLIA1), loricrin (LOR), aquaporin-3 (AQP3), and filaggrin (FLG). Additionally, NF significantly upregulated the expression of hyaluronan synthase (HAS) -1 and − 2 and human β-defensin (HBD) -2 and − 3, which are important for skin hydration and innate immune defense. These findings underscore the potential therapeutic applications of Nypa fruticans (NF) in mitigating oxidative stress, inflammation, skin barrier dysfunction, dehydration, and microbial imbalances. By targeting multiple pathways implicated in skin aging, NF represents a promising comprehensive approach for preserving skin health and addressing age-related dermatological conditions. Moreover, NF holds significant potential not only to alleviate the manifestations of skin aging but also to provide a basis for the development of innovative dermatological therapies. Future investigations should aim to further elucidate the clinical applications of NF in dermatology to maximize its therapeutic benefits.

Facial assessment methods for inhibiting glycation and aging effects and the correlation between glycation and aging parameters

This study aimed to develop in vivo methods for assessing facial anti-glycation and anti-aging effects and to investigate the link between glycation and aging signs. We utilized an AGE reader to measure AGEs levels on the face and arms, establishing a correlation to validate the reader’s use for facial AGEs detection. Then the product’s 7-day anti-glycation effect was evaluated. And its 56-day anti-aging effects were evaluated using non-invasive probes and VISIA CR, assessing skin tone (skin brightness L* and yellowness b*, skin gloss), texture (skin elasticity R2 and firmness F4, skin pores), and hydration (skin moisture content). Correlations between facial AGEs levels and aging parameters were analyzed. Results indicated a strong correlation between facial and arm AGEs after product application, supporting facial AGEs level as an assessment parameter. The product demonstrated substantial anti-glycation and anti-aging effects, suggesting the methods’ efficacy for cosmetic evaluation. A stronger overall correlation was found between AGEs levels and skin tone than with skin texture and hydration, highlighting glycation’s impact on skin color.

Efficacy and safety of topical cream containing aminoacryl tRNA synthetase complex interacting 1-derived peptide in mitigating radiation dermatitis after adjuvant radiotherapy for breast cancer

PurposeAcute radiation dermatitis (ARD) is a frequent side effect experienced by breast cancer patients undergoing radiotherapy. This study aimed to assess the efficacy and safety of a topical cream containing aminoacryl tRNA synthetase complex interacting 1 (AIMP1)-derived peptide (AdP) in mitigating radiation dermatitis (RD) in breast cancer patients undergoing radiotherapy.MethodsAn 8-week single-center, prospective pilot study was conducted to compare the clinical efficacy and safety of an AdP-containing cream with a control cream lacking AdP for the mitigation of RD. Fifteen patients undergoing radiotherapy applied the test cream to the right side and the control cream to the left side of the radiation exposure site, bisected by the nipple line. RD was evaluated at baseline and at weeks 2, 4, 6, and 8, employing the 5-point grading system advocated by the Radiation Oncology Group (RTOG).ResultsThe average RTOG score was lower on the test side in comparison to the control side, and a less pronounced increase in melanin index was observed on the test side. However, these differences were not statistically significant. Both sides exhibited increased skin hydration and decreased transepidermal water loss. Analyzing the maximum RTOG scores throughout the study, RD of maximum grades 1 and 2 was noted in 54.5% and 45.5% of patients on the test side. On the control side, the maximum grades 1 and 2 were observed in 45.5% and 54.5% of patients respectively.ConclusionThe AdP-containing cream did not prove to be more effective than the control cream without AdP in mitigating RD. However, the total incidence of RD in our study was notably lower than previously documented, illustrating the protective effects of both the test and control creams.

Skin Hydration by Natural Moisturizing Factors, a Story of H-Bond Networking.

Dry skin is a common condition that is experienced by many. Besides being particularly present during the cold season, various diseases exist all year round, leading to localized xerosis. To prevent it, the skin is provided with natural moisturizing factors (NMFs). They are small amino acids or derivatives found in the outermost layer of the skin, the stratum corneum (SC). They are often claimed to be highly efficient humectants, increasing the water content to maintain the fluidity of the skin. However, alternative mechanisms have been proposed, suggesting that NMFs themselves may act as lipid mobility amplifiers. This work aims at investigating the role of three NMFs, namely, urea (URE), glycerol (GLY), and urocanic acid/urocanate (UCA/UCO) in SC in silico models, considering two different levels of humidity. Molecular dynamic simulations showed an increase in the diffusion of different lipid components, mainly free fatty acids (FFAs) and ceramide acyl chain moieties, in the presence of either high water content or NMFs. The membrane properties were modified, as seen by an increased thickness and greater lateral stiffness. All NMFs exhibited a similar impact, whereas UCA revealed slight differences according to its charged state. By studying NMF-water intermolecular interactions, we highlighted the role of NMF as a regulator of membrane perturbations while ensuring membrane fluidity. This role allows NMFs to prevent destabilization of the skin membrane in the presence of high water content. This study, performed at an atomistic resolution, highlighted a strong H-bond network between lipids involving mainly ceramides but also all other components. This network can be modified in the presence of a high water concentration or NMFs, resulting in modifications of membrane properties, rationalizing hydration effects.

In vivo efficacy of a stabilized Vitamin C-based serum at pH 6 on some ageing facial signs of women of different ages and phototypes.

To assess, clinically and instrumentally, the efficacy of a stabilized 12% Vitamin C (VC)-based serum (pH 6) on skin photoageing of women of various age and phototype. Eighty women, with Phototypes I to VI, of various age (45-70y) living in Mauritius, participated in an open, uncontrolled study. After a two-week period of washout, they applied the Vitamin C-based serum twice a day for 2 months onto their whole face as well as a hydrating cream every evening to ensure a comparable skin hydration level in all subjects. At set time intervals (D0, D immediate, D14, D28 and D56), a local dermatologist graded eight facial signs by visual or tactile assessments, using a 0-9 structured scale. At each time interval, the facial skin was imaged by standardized technique (Colorface™) and through echography (Dermascan™) to record its thickness and density. Skin elasticity was recorded by a the Cutometer® device under a controlled suctional stress. A questionnaire was filled out by all subjects recording their self-assessments of the serum's effects and several organoleptic properties. Compared with the baseline values (D0), the skin of all subjects showed progressive and significant changes in glow, tone evenness, radiance, firmness, softness and smoothness, associated with a reduction in fine lines. Deep wrinkles, only observed on 26 subjects, were found to be reduced. Self-assessments corresponded well with both clinical and instrumental results and indicated excellent skin tolerance. Ultrasound recordings exhibited significantly thicker and denser skin at D56. If certain changes in some skin facial signs (skin tone and related items, firmness, sagging…) are linked to the high concentration of VC, a complementary impact of increased skin hydration remains possible due to the glycerol present in the serum and hydrating cream. These significant performance results invivo showed that a very acidic medium (∼pH 3) is not a prerequisite to an efficient VC cosmetic formula, as has long been accepted in the cosmetics industry. Such a low pH can fragilize the skin equilibrium over time. As second originality, the studied formula seemed to fit all phototypes.

Lipidomic and Instrumental Evaluation of a Melatonin-Based In & Out Strategy Versus Topical Treatment in Skin Aging: A Randomized Prospective Trial.

This study aimed to evaluate the efficacy of a novel "In & Out" strategy, combining topical and oral melatonin supplementation, in managing skin aging compared to topical treatment alone. A randomized, prospective study was conducted on 39 healthy females aged 55-69 years. Participants were divided into two groups: one received both the topical formula and oral melatonin supplementation (Group A), while the other received a topical melatonin-based formula (Group B). Clinical evaluations included lipidomic analysis, skin moisturization, and wrinkle depth analysis at baseline and after 84 days. The addition of oral melatonin supplementation to the topical regimen led to improvements in the skin's lipid profile and moisturization levels. These findings suggest that combining topical and oral melatonin may provide a more comprehensive approach to managing skin aging by addressing both local and systemic factors. Background/Objectives: With age, the endogenous antioxidant capacity of the skin decreases, including melatonin (Mel) synthesis. Skin aging is also associated with alterations in epidermal lipids, particularly a reduction in triglycerides and ceramides, which are essential for maintaining skin structure and hydration. The administration of exogenous melatonin could, therefore, be an effective anti-aging strategy. While some data suggest that melatonin may positively influence the lipid profile, specific data on its effects on skin aging are lacking. This study aimed to evaluate the anti-aging effects of an "In & Out" regimen consisting of a Mel-based cream and dietary supplement in comparison with topical treatment alone, focusing on clinical and lipidomic changes involved in skin homeostasis. Results: A statistically significant variation was observed in both groups compared to baseline (T0) in terms of moisturization (+23.6% in Group A, +18.3% in Group B) and wrinkle depth (-18.5% in Group A, -9.4% in Group B, p < 0.05). Both groups showed improvements in the lipid content of the skin, which typically decreases with age. The "In & Out" strategy resulted in a statistically significant increase in triacylglycerols and ceramides, key lipids that exhibit water-holding properties. Conclusions: The "In & Out" melatonin-based regimen demonstrated greater efficacy in clinical improvement and positive lipid profile modifications compared to topical treatment alone, highlighting its potential as a comprehensive anti-aging strategy.

Clinical efficacy of OS-01 peptide formulation in reducing the signs of periorbital skin aging.

The aging of the skin, particularly around the periorbital region, is a complex process characterized by the accumulation of senescent cells, decreased collagen production, and reduced skin elasticity, leading to visible signs such as fine lines, wrinkles, and sagging. This study investigates the efficacy of a novel topical formulation, OS-01 EYE, containing the senomorphic peptide, OS-01, along with other active ingredients, in improving the skin around the eyes. A 12-week clinical study was conducted with 22 participants who applied OS-01 EYE twice daily. Assessments included bioinstrumental measurements of skin hydration, barrier function, firmness, and elasticity, as well as expert photographs grading fine lines, wrinkles, under-eye puffiness, and dark circles. After 12 weeks of product use, transepidermal water loss (TEWL) decreased by 17.33%, hydration increased by 32.49%, skin firmness improved by 10.19%, and elasticity increased by 25.58%, compared to baseline, which was all statistically significant. Expert grading revealed a decrease in fine lines and wrinkles, under-eye puffiness, and dark circles over the study period. Furthermore, subjective assessments showed that 95.46% of participants reported improvements in overall appearance. The OS-01 EYE formulation was found to be safe and effective in mitigating the visible signs of aging in the periorbital region, making it a potent treatment option for enhancing periorbital skin health, function, and appearance.

Potential of Cissampelos pareira L. Pectin as a Bioactive Compound in Moisturizing and Anti-Aging Applications

Cissampelos pareira is a plant known for its edible and medicinal properties, with bioactive potential in various applications. This study aimed to isolate and characterize pectin from C. pareira (PCP) and assess its safety, as well as its efficacy in skin moisturizing and anti-aging effects on human volunteers. The highest yield of PCP extraction was achieved after a 45 min extraction period. Chemical composition analysis using Fourier transform infrared (FT-IR) spectroscopy confirmed the presence of a characteristic pectin structure. Optimized PCP samples revealed high methoxy pectin content, degree of esterification, and galacturonic acid concentration, along with consistent physicochemical properties. PCP exhibited antioxidant activity and showed no cytotoxicity at 1 mg/mL, and effectively reduced hydrogen peroxide-induced oxidative damage in NIH/3T3 cells. Stability assessment indicated that PCP maintains its physical appearance and total polysaccharide content after heating–cooling periods. None of the PCP formulations caused skin irritation among volunteers. In an 8-week clinical trial, subjects who used serum formulations containing 0.5% w/w and 1.0% w/w PCP showed significant improvements in skin hydration and elasticity compared to the placebo serum group. While skin smoothness, scaliness, and wrinkling also improved, these anti-aging results were not statistically significant. The results indicated PCP is a promising candidate for skin care applications due to its safety, stability, antioxidant activity, and demonstrated efficacy in enhancing skin hydration and elasticity.

Evaluation of operational parameters for clinical evaluation of skin hydration by corneometry method

Abstract The Corneometer CM® 825 from Courage &amp; Khazaka electronic GmbH is widely utilized in clinical efficacy testing of cosmetics. Assessing methodological variables is crucial for ensuring the reliability of the process. The aim of this study was to evaluate the operational and instrumental parameters of the Corneometer CM® 825 in the clinical assessment of skin hydration levels. Precision and accuracy of the measurements were assessed by measuring hydration levels on 10 participants in specific areas of the forearm and face. Three different skin conditions (dry, normal, and hydrated) resulting from the use or non-use of moisturizing cosmetics and a drying promoter were evaluated within 120 minutes of their application. The electrical capacitance method demonstrated good repeatability, with coefficients of variation mostly below 10%. However, higher variability was observed in inter-observer readings. The equipment exhibited high sensitivity and accurately discriminated the differences between hydrated and dry skin. Operational training and standardization of the methodology are essential to ensure accurate, reproducible, and reliable results in studies utilizing the Corneometer equipment for assessing skin hydration levels.

κ-Carrageenan tetrasaccharide ameliorates particulate matter-induced defects in skin hydration of human keratinocytes cells and skin barrier disorders

κ-Carrageenan tetrasaccharide ameliorates particulate matter-induced defects in skin hydration of human keratinocytes cells and skin barrier disorders

Skin Hydration Augmentation Following Solar Protective and Repair Skincare Regimen

Skin Hydration Augmentation Following Solar Protective and Repair Skincare Regimen

Evaluation of the Effectiveness of Using LED Light Combined With Chromophore Gel in Treating Acne Vulgaris - Preliminary Study.

The aim was to quantitatively evaluate the effectiveness of LED light therapy combined with photoacceptor substances having anti-acne properties in reducing the symptoms of acne vulgaris. 15 subjects aged 20 to 24 who suffered from moderate or severe acne lesions. The treatments were performed using a LED device (465-880 nm). Blue light - 465 nm in combination with red light - 640 nm in pulsed mode and near-infrared light - 880 nm were used in the treatments. Each patient underwent 6 treatments, with intervals between treatments of 7 days. 3D images and biomechanical measurements were performed before first and fourth treatments and 6weeks after the last. Additionally, photographic documentation was made 7 days after the 6th treatment. The series of treatments significantly influenced the clinical condition of the patients' skin. The treatments had a statistically significant impact on the intensity of erythema on the left and right cheeks. The hydration of the stratum corneum in all the examined areas of the face changed significantly after the treatments. Greater hydration was achieved six weeks after six treatments compared to the values obtained prior to treatment and after three treatments. The activity of sebaceous glands and, consequently, the amount of sebum on the epidermal surface in all the examined areas of the face changed significantly after the treatments. The treatments had a statistically significant impact on the volume of atrophic scars. Therapy combining LED light with photoacceptor substances is an effective method of reducing the symptoms of acne vulgaris. The treatments lead to a significant reduction in erythema, reduction in the volume of atrophic scars, improvement of skin hydration and regulation of sebum secretion. These effects can be identified quantitatively. Further studies are necessary to confirm the long-term effectiveness and safety of this method.

Clinical evaluation of a topical ceramide lotion on skin hydration and skin barrier in healthy volunteers with dry skin

Objectives: Ceramides are epidermal lipids important for normal skin barrier function. Reduced ceramide content is associated with dry skin and atopic dermatitis (AD) due to increased transepidermal water loss (TEWL) and worsening symptoms. Topical ceramide application restores barrier function and improves hydration by reducing TEWL. The objective of the present study was to evaluate the effect of a test product on skin hydration and TEWL on the volar forearms of adults, by comparing measurements at 12 and 24 h post-application. Materials and Methods: This single-center and non-randomized study evaluated the effect of Venusia CeraPlus lotion on volar forearms in volunteers with dry skin. Primary outcomes included MoistureMeterSC reading for hydration and VapoMeter reading for TEWL at 12- and 24-h post-application, comparing occluded and unoccluded sites. Results: The study enrolled 32 subjects with a mean age of 34.03 ± 9.41 years, out of which 87.5% were female. Application of Venusia CeraPlus lotion enhanced skin hydration compared to the control under both occluded and unoccluded conditions. Hydration increased from 13.57 ± 2.67 at baseline to 31.61 ± 11.34 at 12 h and 36.36 ± 10.77 at 24 h under occlusion (P &lt; 0.001). TEWL was lower at the test site, with VapoMeter readings of 3.08 ± 2.32 at 24 h compared to 4.54 ± 2.76 at the control site, suggesting that the test product effectively preserved skin hydration and reduced TEWL compared to the control. No adverse reactions were reported. Conclusion: This study demonstrates that Venusia CeraPlus Lotion effectively maintains optimal skin hydration for up to 24 h and enhances barrier function, making it a promising option for treating dry skin and improving overall skin health in adults.

Munir Rashid Chowdhury

Background: Acne vulgaris is a common dermatological condition influenced by factors such as pore blockage, excessive sebum production, and inflammation. This study evaluates the comedogenicity, skin tolerability, and functional benefits of Siodil acne treatment products, including Sebi Gel, Sebi Serum, Sebi Cleanser, and ATO Gel. Methods: A total of 26 participants aged 18–40 years underwent a 28-day open-label trial to assess the impact of Siodil products on pore blockage, skin hydration, and acne reduction. Comedogenicity was scored using a standardized acne grading scale, and patch tests were conducted to evaluate erythema and edema. Participant satisfaction and product tolerability were also assessed. Results: All products demonstrated significant improvements in skin hydration, with ATO Gel showing the highest improvement (32.47%), followed by Sebi Gel (30.56%). Acne breakout reduction was highest for Sebi Cleanser (85%), while Sebi Serum showed the greatest whitehead/blackhead reduction (76.92%). Patch tests revealed no occurrences of erythema or edema at 24 and 48 hours. Participant feedback indicated high satisfaction, with Sebi Gel receiving the highest satisfaction rating (90%). Conclusion: Siodil acne treatment products effectively reduce acne-related outcomes while improving skin hydration and demonstrating excellent tolerability. These findings suggest Siodil products are safe and effective for managing acne-prone skin, though further research is warranted to confirm these results in larger populations.

Assessment of Skin Condition and Efficacy of Liquid and Cream Hand Sanitizers

Hand sanitizer has become a popular alternative to traditional hand washing due to its ease of use, convenience, and effectiveness. Most hand sanitizers on the market are formulated with high concentrations of alcohol and lack of moisturizing agents, which can cause skin problems by altering the skin barrier's integrity and function with repeated use. To counteract these drying and irritating effects, a moisturizing hand sanitizer cream was developed. This study aims to assess the moisturizing efficacy of the developed cream and compare skin condition and antimicrobial effectiveness after using two different forms of hand sanitizer. The in vivo study involved comparing skin conditions after two weeks of using the developed moisturizing hand sanitizer cream and a commercial hand sanitizer liquid. Biophysical parameters such as trans epidermal water loss (TEWL), skin hydration, and elasticity were measured. Fifteen volunteers completed the study. Results showed a significant decrease in TEWL for the hand sanitizer liquid, but a gradual decrease for the cream. Skin hydration significantly decreased with the liquid but increased with the cream. Additionally, skin elasticity significantly improved with the cream compared to the liquid. Microbiological analysis indicated some contamination, but the hand sanitizer cream generally was more effective in reducing bacterial and fungal counts compared to the liquid sanitizer. In conclusion, these findings suggest that the moisturizing hand sanitizer cream not only supports skin health but also provides effective antimicrobial protection, highlighting its potential as a beneficial alternative to traditional hand sanitizers.