Hybrid Status Research Articles

Care Facilities for Older People With Long-Term Substance Use: Promising Practices From Sweden

The stigma of alcohol and long-term substance use is well-known and may be even greater for older people. This is a presentation on “wet” eldercare facilities, i.e. care settings designed for older people with long-term substance use problems, where abstinence is abandoned for well-being. Wet eldercare facilities exist in several European countries and the Swedish ones have a hybrid formal organization: They target people over 50 years, but are regarded as nursing homes and residents lease their own flats inside the setting, which makes it correct to describe residents as tenants. Guided by symbolic interactionism, the aim is to analyze how residents in wet eldercare facilities manage to view these places in a positive light. Forty-two residents of four facilities were interviewed, revealing how the hybrid status of these places enabled residents to frame their situation as being “in the right place”, but for different reasons. Some framed the place as a nursing home, others as an ordinary flat. Although wet eldercare facilities are undisputedly linked to stigma and the inability to become sober, the formal hybrid organization enabled residents to construct less stigmatized characterizations of the place and of themselves. The study suggests that it is an (often-neglected) gerontological responsibility to counter stigma and improve the sense of dignity for older people living in stigmatized settings. Based on promising practices in the Swedish system, the study therefore presents strategies that enable older people to ascribe positive characteristics to themselves and to the place where they live.

Of men and beasts: an anthropological analysis of the movie "Men and Chicken"

Following the path established in Serbian anthropology by the subdiscipline of the anthropology of film, as well as the anthropological "section" for the study of science fiction, the analytical focus of this paper is the film Men and Chicken. Contrary to critics who saw in this film "an unreadable, morbid and cynical postmodern horror", the basic thesis of this paper is that the film helps us not only to question public ideas about science, but also to shed light on the hopes, fears and conceptual dilemmas caused by the advance of biotechnologies. It helps us to, using a product of popular culture close to the experience of the general public, analyze the way in which the imaginarium of the film deals with the "paradox at the heart" of western thought - the simultaneous claim that humans are animals and that animality is the polar opposite of what we hold to be human. The paradox in question need not be an intellectual chew toy for a bored mind, nor an opportunity for demonstrating the wondrous power of an analytical process of Levi-Straussian calibre, rather a very relevant question in current institutional and public discussions on creating hybrid embryos/species at the heart of which are attempts to establish the biological, moral and cultural status of humans, non-humans and hybrids.

Who are the elephants living in the hybridization zone? How genetics may guide conservation to better protect endangered elephants

After a long-standing debate, African elephants are now considered by the IUCN as two distinct species: savannah elephants ( Loxodonta africana ), listed as endangered on the IUCN Red List of Threatened Species, and forest elephants ( Loxodonta cyclotis ), critically endangered. Both are severely threatened by forest loss, fragmentation and degradation due to agriculture expansion, as well as by illegal ivory trade. Although the two species have different habitat preferences, their range overlaps in some ecotones; despite an ancient separation between these two species, hybrids have been reported in five locations. The main hybrid hotspot is located on the Democratic Republic of Congo-Uganda border and still remains understudied. Using 15 microsatellites, we investigated this hybridization zone by determining the species and hybrid status of 177 fecal samples collected in the area of Sebitoli, at the extreme North of Kibale National Park. Surprisingly for a forest area, no pure forest elephants were detected. Out of the 91 individuals sampled, a very large proportion (81.3%) were hybrid individuals mainly from a second generation or more. Only 18.7% of pure savannah elephants were detected, all originating from the DRC-Uganda border. Further analyses are necessary to assess the age of this hybridization zone. Our results emphasize that hybrids and savannah elephants can successfully range in forested area. They also show that forest elephants are rare even in their native habitat. In the current context of high threat faced by African elephant species, it is crucial to strengthen conservation efforts for these species before it is too late. • 91 individuals were sampled in the Ugandan elephant hybridization zone. • No forest elephants were detected in the sampled forested area. • 81% of the sampled elephants were hybrids. • Hybrids are fertile with 98.6% of hybrids belonging to second generation or more. • Pure savannah elephants seem to originate from the DRC-Uganda border.

Гибрид подсолнечника Статус

The widespread saturation of crop rotations with sunflower crops contributed to the creation of favorable conditions for the emergence and wide distribution of new more aggressive races of broomrape, downy mildew and other pathogens of this crop. Sunflower varieties and hybrids resistant to previously common races have become severely affected. One of the ways to solve this problem is to develop sunflower hybrids resistant to new virulent races. The breeders had to combine high productivity and genes of resistance to new more aggressive races of broomrape and downy mildew in one genotype. The first stage of the work was development of a new breeding germplasm and selection of parental lines with breeding valuable traits from it. Based on these lines, there were developed hybrid combinations resistant to new highly virulent races of broomrape and downy mildew. The hybrid Status was developed by hybridization of a maternal line ED 47 and a paternal line ED 193. The maternal line ED 47 is single-headed, low height, resistant to the broomrape races: E, F, G, has a good combining ability. The paternal line ED 193 is branched, also resistant to broomrape (races E, F, G) and three races of downy mildew (330, 710, and 730). The hybrid Status belongs to the middle-early maturity group, duration of the growing season is 95 days. The average plant height over the years of testing was 140 cm; the plants are uniformed in height and the duration of the development phases. Seed yield of the hybrid Status exceeded the standard one (a hybrid Gorstar) by 0.25 t/ha, and oil yield – by 0.14 t/ha. The oil content of the seeds was 48.0%, the huskiness – 28.7%, the 1000 seeds weight – 60.6 g. The new hybrid is resistant to seven races of broomrape (A, B, C, D, E, F, and G) and three races of downy mildew (330, 710, and 730). A distinctive feature of it is resistance of both parent lines to broomrape. The originator of a simple interline sunflower hybrid Status is V.S. Pustovoit All-Russian Research Institute of Oil Crops.

Culture, Technology and the New Death: Deadly Disputes in Japan and North America

"Brain death" was institutionalized 25 years ago primarily to make organs "harvested" from brain dead bodies on an artificial ventilator available for transplant. Doubts exist as to whether the donors used in the first heart transplants conducted in South Africa in 1967, and in Japan in 1968, were dead. Reaction to these two experiments was remarkably different. In Japan the surgeon was arrested for murder, a national debate about brain death continues today, and transplants from brain dead bodies have only been made permissible since June 1997. This debate contrasts with that in North America, where transplant technology is routinized and an organ "shortage" is recognized. In Japan the argument is politicized, focuses on death, and draws on cultural knowledge about Self and Other; Japan and the West; harmony and individualism; tradition and modernity / postmodernity. North American discussion focuses on saving lives; making death meaningful; remaking death yet again; and assumes technology to be culturally and politically autonomous. These respective debates are discussed using textual analysis and the results of interviews with philosophers, physicians, and nurses in both cultural settings. An effort is made to suggest why the Japanese debate is central in constituting contemporary cultural identity, whereas the North American debate is of little public interest. The significance for anthropology of the hybrid status of brain dead bodies and organ recipients together with the national and global circulation and commodification of bodies and body parts associated with this technology is considered.

HER2 Amplification by Next-Generation Sequencing in Lung Carcinoma: A Comparison of NGS Amplified and Non-amplified Cases by Immunohistochemistry and In Situ Hybridization

Background:Although the role of HER2 amplification and its evaluation methods are well known in breast carcinoma, methods for detection of HER2 amplification in non-small cell lung carcinoma are unclear. Next-generation sequencing is widely used in searching multiple therapeutic targets, and it is possible to evaluate copy number variation of genes by next-generation sequencing.Aims:To re-evaluate the HER2 status of non-small cell lung carcinoma cases detected as HER2 amplified and non-amplified by next-generation sequencing via the most commonly used HER2 investigation methods in routine pathology practice, namely immunohistochemistry and in situ hybridization.Study Design:Retrospective cross-sectional study.Methods:Among the 256 patients whose mutation profiles were examined by next-generation sequencing, HER2 amplified (13 cases) and non-HER2-amplified (13 cases) were determined as study and control groups, respectively, by next-generation sequencing. HER2 next-generation sequencing amplified tumors were investigated for HER2 expression and amplification using immunohistochemistry and silver in situ hybridization.Results:From a group of 256 non-small cell lung carcinoma, 33 tumors (12.8%) showed HER2 amplification with next-generation sequencing. Although we observed more frequent HER2 positivity by immunohistochemistry in next-generation sequencing-amplified cases, when compared to non-amplified cases (50% and 23% respectively), the difference was not significant (P = .221). Within the HER2 amplified group, inter-method-agreement was very good between next-generation sequencing results amplification and in situ hybridization status. Next-generation sequencing results showed a strong interclass correlation coefficient with HER2/cell (P = .009, r = 0.777) and HER2/CEP17 ratio (P = .001, r = 0.805). The median HER2/CEP17 ratio was higher in the next-generation sequencing amplified group (P = .013); however, three cases were found to be amplified by silver in situ hybridization among the next-generation sequencing non-amplified cases. EGFR and FGFR1 amplification were more frequent in HER2 next-generation sequencing amplified group than next-generation sequencing non-amplified group (P < .001).Conclusion:Until the effects of HER2 amplification on the HER2 protein are well understood and pulmonary carcinoma algorithms are defined, non-small cell lung carcinomas found to be amplified by next-generation sequencing should be verified by additional methods.

Evaluation of Complete Fertilizer in the Aspect of the Antioxidant Enzyme System of Maize Hybrids

Studies on physiological and biochemical processes in crops are highly relevant for breeders to produce hybrids with high yield. Two different maturity groups of maize hybrids were tested in this study. The research site was located at the Látókép Experimental Station of the University of Debrecen and the experiment lasted for 2 years. The examined nitrogen ranges were separated into two parts. Firstly, the effects of nitrogen fertilizer ranging from 120–300 kg ha−1 were examined, supplemented with a constant, high-level P2O5 and K2O. Secondly, the optimal ratio of N:P:K was measured. In order to monitor the health status of maize hybrids, stress indicators including the activity of ascorbate peroxidase (APX), and superoxide dismutase (SOD), the rate of lipid peroxidation (LP), and grain yield were measured. The samples were taken in five phenological stages. Variance analysis based on nitrogen fertilizer showed variation in sampling times and fertilizers on APX, LP, and SOD. Variance analysis based on NPK indicated variation in sampling times, years, and fertilizer levels on APX, LP, and SOD. Correlation analysis showed that yield correlated negatively with SOD during the use of NPK fertilizer, as the use of nitrogen fertilizer cannot make corrections to yield with SOD but phosphorus and potassium can correlate with yield, and SOD. Principal component analysis showed that NPK5 and N5 had maximum stability and effect on yield. The activity of APX had the highest value during silking, and LP was in the V14 leaf stage. The correlation and principal component analysis showed that silking and the V14 leaf stage are the most important stages for yield, thus, higher attention must be paid to these stages in the LP and the activation of APX to achieve maximum yield.

Maize yield performance altered by trinexapac-ethyl rates and plant population

Increases in plant density associated with the use of plant growth regulators, aside from modifying the plant architecture, can also change the maize yield performance. This study aims to investigate the yield components and grain yield of maize hybrids with contrasting leaf architecture, in response to different plant populations and trinexapac-ethyl rates (TE). Field experiments were conducted in two growing seasons, in a (5 x 5) factorial randomized block design. The treatments consisted of: five plant population (40, 60, 80, 100, and 120 thousand plants ha-1) and five TE rates (0, 100, 200, 300, and 400 g a.i. ha-1), with four replications, using the hybrids 2B710 HX and Status TL. We evaluated the number of rows per ear, ear length, number of grains per row, 1000-grain mass, and yield. An increased plant density reduces ear length, number of grains per row and 1000-grain mass, without changing the number of grain rows per ear. TE rates increases ear length and number of grains per row and reduces 1000-grain mass, mainly under favorable cultivation conditions. TE interacts with the plant density, changing the maize yield components with increases in grain yield, but these gains variy, according to the environment and genotype characteristics.

Genetic Signature of a Past Anthropogenic Transportation of a Far-Eastern Endemic Cladoceran (Crustacea: Daphniidae) to the Volga Basin

Most studies of water flea (Crustacea: Cladocera) invasions are concentrated on a few taxa with an obvious harmful influence on native ecosystems, while our knowledge of cases of anthropogenic introduction with not-so-obvious consequences, in most other taxa, is poor. We found in the Volga basin (European Russia) a population that contained D. curvirostris Eylmann, 1887 and its hybrids with D. korovchinskyi Kotov et al. 2021. The latter taxon is endemic to the Far East and it has appeared in the Volga basin as a result of past human-mediated transportation. The population from Bakhilovo is represented by two strongly different groups of the COI haplotypes belonging, respectively, to (1) D. curvirostris and (2) D. korovchinskyi. We detected SNPs in the position 60 of the HSP-90ex3 locus and in the 195 positions of 28S rRNA locus, which differentiate two species. Part of the specimens from Bakhilovo belonged to D. curvirostris s.str., demonstrating homozygote SNP sites in two loci, but two specimens had heterozygote SNP sites in both nuclear loci. They belong to D. curvirostris x korovchinskyi hybrids. Most morphological traits of the females were characteristic of D. curvirostris. We found in some specimens some characters which could suggest their hybrid status, but this opinion is a hypothesis only, which needs to be checked on more ample material. The exact hybrid system in this pond is not known. Moreover, we have no evidences of sexual reproduction of the hybrids; they could reproduce by parthenogenesis only as is known for hybrids of the D. pulex group, or continuously crossing with parents like some members of D. longispina group. However, poor parental D. korovchinskyi was not detected in the pond either morphologically or genetically. The exact vector of its past anthropogenic transportation to the Volga is unknown. Most probably, just ephippia of D. korovchinskyi were translocated replaced from the Khabarovsk Territory to the Samara Area somehow. This is the first report on hybrids within the D. curvirostris species complex. Here, we demonstrated that accurate studies with deep resolution increase the number of revealed cryptic invasions. We expect that the number of revealed cases of cryptic interspecific invasions will grow rapidly.

Tank mixture of glyphosate and 2,4-D applied on RR® maize crop

Herbicide tank-mixing is often carried out by farmers aiming to control weeds in crops. Thus, it is important to evaluate the effects of these mixtures regarding the selectivity of crops. The aim of this work is to assess the selectivity of increasing doses of herbicides (glyphosate and 2,4-D), applied alone or as a mixture, on maize crop. Two experiments were carried out in randomized blocks, with four replications, during two crop years. The evaluated variables were: phytotoxicity, plant height, stem diameter, ear length, number of rows per ear, number of grains per row, mass of 1,000 grains and grain yield. The herbicide, 2,4-D, when applied in mixture with glyphosate caused severe phytotoxicity symptoms on maize. Grain yield and growth characteristics were not affected by the herbicides applied alone or as a mixture. Increasing doses of 2,4-D caused severe phytotoxicity symptoms on hybrid Status VIP3. Increasing doses of glyphosate did not show phytotoxicity symptoms and are not directly correlated to any injury caused by 2,4-D in the SX 5885 VIP3 maize hybrid.

Breeding Aspects of Selected Ornamental Bulbous Crops

This article provides an overview of the origin, genetic diversity and methods and trends in breeding of selected ornamental geophytes (Lilium, Tulipa, Narcissus and Hippeastrum). The role of interspecific hybridisation and polyploidisation in assortment development is reviewed. A great variety of cultivars with traits of interest have been generated over the last century by using classical breeding. Geophyte breeders have been interested in a diversity of traits, including resistance to diseases, flower colour and shape, long lasting flowering and a long vase life. Shortening the long breeding process of many geophytes by reducing the juvenile phase and using in vitro techniques are reviewed. Currently, the breeding process has been enhanced by using modern molecular cytogenetic techniques. Genomic in situ hybridisation is frequently used, among other techniques, for genome differentiation in interspecific hybrids, and for assessment of the extent of intergenomic recombination in backcross progenies. Furthermore, several molecular marker techniques are used for verification of hybrid status, identification of genetic diversity, confirmation of the genetic fidelity of in vitro propagated plants and construction of high-density linkage maps. Recently, a myriad of new plant breeding technologies, such as cisgenetics and genome editing technologies have been used to improve the traits of ornamental geophytes, an endeavour that is discussed here. Breeding trends, cultivar novelties as well a new cultivars registered by international authorities during the last five years are presented in detail.

LESS CARE, MORE STRESS: A RHYTHMIC POEM FROM THE ROMAN EMPIRE

This article considers a short text that was widely circulated in the mid- Roman Empire, in both a four-line and a six-line version, usually on gemstones. The text is a poem of sorts, but of a quite distinctive type. Part of it can be scanned according to the rules of classical (quantitative) metre, but more striking is the consistent rhythmic (stressed) pattern. Stressed poetry is not otherwise attested so early; this text may point to a substrate, now largely hidden from view, of popular verse that preceded the metrical revolutions of late antiquity and the Byzantine world. The poem is also a piece of visual artistry, designed to be looked at (particularly in its gemstone format). This hybrid status, between high art and popular culture, can also be detected in the content of the poem, which gestures towards both the poetics of intellectual elitism (using intertextual allusion, and dismissing the views of the masses) and a level of sexually aggressive assertion of embodied selfhood. It is a valuable witness to a form of middling literature (and a middling demographic), caught between aspirations to elite-style individuality and the mimetic imperative of an empire-wide consumer culture.

Ivan Sokolov and his post-mortem studies of the “Hairy Woman” Julia Pastrana and her son

In this article we document the role of Ivan Matveevich Sokolov, anatomy professor at Moscow University, in the mummification of Julia Pastrana, born in Mexico (afterwards an American citizen by marriage), and her son. Sokolov had investigated and described the corpse of this famous “hairy woman” as an example of a congenital anomaly of the genus Homo. Due to the art of Sokolov’s embalming, the mummies of Julia and her son were presented to the scientific world, which made it possible to study similar cases of deformity in the human population. However, the historical role of Sokolov was not limited to his study of a congenital disease. His thorough postmortem examination and description of Pastrana’s and her son’s bodies allowed Sokolov to make an indirect contribution to evolutionary thought. Sokolov’s confirmation that Pastrana belonged to the genus Homo refuted all speculation about her hybrid origins and status as a missing link in the evolution of apes into humans.

Computed tomography radiomics to predict EBER positivity in Epstein-Barr virus-associated gastric adenocarcinomas: a retrospective study.

The relevance of Epstein-Barr virus (EBV) in gastric carcinoma has been represented by the existence of EBV-encoded small RNA (EBER) in the tumor cells and has prognostic significance in gastric cancer, while gastric adenocarcinoma represents the most frequently occurring gastric malignancy. To observe the capacity of radiomic features extracted from contrast-enhanced computed tomography (CE-CT) images to differentiate EBER-positive gastric adenocarcinoma from EBER-negative ones. A total of 54 patients with gastric adenocarcinoma (EBER-positive: 27, EBER-negative: 27) were retrospectively examined. Radiomic imaging features were extracted from all regions of interest (ROI) delineated by two experienced radiologists on late arterial phase CT images. We distinguished related radiomic features through the two-tailed t test and applied them to construct a decision tree model to evaluate whether EBER in situ hybridization positive had appeared. Nine radiomics features were significantly related to EBER in situ hybridization status (P < 0.05), four of which were used to build the decision tree through backward elimination: Correlation_ AllDirection_offset7, Correlation_ angle135_offset7, RunLengthNonuniformity_ AllDirection_offset1_SD, and HighGreyLevelRunEmphasis_ AllDiretion_offset1_SD. The decision tree model consisted of seven decision nodes and six terminal nodes, three of which demonstrated positive EBER in situ hybridization. The specificity, sensitivity, and accuracy of the model were 84%, 80%, and 81.7%, respectively. The area under the curve of the decision tree model was 0.87. Radiomics based on CE-CT could be applied to predict EBER in situ hybridization status preoperatively in patients with gastric adenocarcinoma.

The trajectory of hybrid and hydrogen technologies in North American heavy haul operations

The central aim of this paper is to provide an up-to-date snapshot of hybrid and hydrogen technology-related developments and activities in the North American heavy haul railway setting, placed in the context of the transportation industry more broadly. An overview of relevant alternative propulsion technologies is provided, including a discussion of applicability to the transportation sector in general and heavy haul freight rail specifically. This is followed by a discussion of current developments and research in alternative and blended fuels, discussed again in both general and specific settings. Key factors and technical considerations for heavy haul applications are reviewed, followed by a discussion of non-technical and human factors that motivate a move toward clean energy in North American Heavy Haul systems. Finally, current project activities are described to provide a clear understanding of both the status and trajectory of hybrid and hydrogen technologies in the established context.

Enabling positive framings of stigmatised settings: a neglected responsibility for social work

ABSTRACT Settings in social work may be associated with spatial stigma. This study uses the case of a highly stigmatised setting to investigate ways of ascribing positive characteristics to tainted contexts. Guided by symbolic interactionism, the aim is to analyse how residents in ‘wet’ eldercare facilities manage to view these in a positive light. Wet eldercare facilities are designed for older people with long-term substance use problems, where abstinence is abandoned for well-being. Forty-two residents of four such facilities were interviewed, revealing how the hybrid status of these places enabled residents to frame their situation as being ‘in the right place’, but for different reasons. Some framed the place as a care home, others as an ordinary flat. Both frames were made credible by the formal hybrid organisation: Swedish wet eldercare facilities are part of the eldercare system, and residents’ rooms are formally regarded as flats. The study suggests that it is social work’s (often neglected) responsibility to counter spatial stigma and improve residents’ sense of dignity. Based on promising practices in the Swedish system, the study presents three strategies that enable residents in nominally tainted settings to ascribe positive characteristics to the place where they live.

Rethinking the systematics of history with Jean Baechler beyond Reinhart Koselleck – the challenge of the three M's (meta, macro, micro)

ABSTRACT The article develops three complementary lines of argument. It first points to the great theoretical gap that characterizes Koselleck's work: quasi-transcendental anthropology on the one hand, and historical semantics on the other. The hybrid status of historical semantics, both macro- and micro- sociological, is also specified. Secondly, the article critiques the neo-Hobbesianism implicit in Koselleck's historical ontology. Departing from Jean Baechler's anthropology, it reformulates the conceptual framework within which the initial foundational ambition could be maintained without however renewing the aporiai of the Koselleckian systematics. Finally, it asks how to connect the three M's (the meta-historical, the macro- and the micro-sociological levels). The solution focuses on the core aspects of the intermediate, macro-sociological level to make the case for a heuristic reciprocal hybridization of meta-historical anthropology, of comparative historical sociology and of the pragmatic history of historical semantics.

Introgression between Sphyrapicus nuchalis and S. varius sapsuckers in a hybrid zone in west‐central Alberta

Studying species interactions at hybrid zones allows biologists to understand the forces that promote speciation. Hybridization among Sphyrapicus nuchalis, S. varius and S. ruber has long been acknowledged, and hybrid zones between S. nuchalis/S. ruber and S. varius/S. ruber have been characterized with both genetic and genomic data. Using a combination of next‐generation restriction site‐associated DNA sequencing (RAD‐Seq) and traditional genetic methods, we examined patterns of introgression in the poorly characterized S. nuchalis/S. varius contact zone; the two most similar species in the complex, though they are not each other's closest relatives. We found high introgression rates, with several early and many advanced generation hybrids along a 275 km stretch of Rocky Mountain foothill, pointing to a well‐established hybrid zone with hybrid individuals backcrossing with individuals from the parental species and each other. Plumage colouration in the hybrid zone was a relatively poor indicator of parental or hybrid status, which could be attributed to the possible involvement of few large effect genes.

A LIKELY INTERSECTIONAL HYBRID IN VACCINIUM (ERICACEAE) ON SAN BRUNO MOUNTAIN, SAN MATEO COUNTY, CALIFORNIA

A population of Vaccinium from San Bruno Mountain in San Mateo County, California has been confused with V. cespitosum Michx. (V. sect. Myrtillus), a species documented from the mountain, since its initial collection in 1961. These plants resemble V. cespitosum and other species in V. sect. Myrtillus in several characters, but differ most notably in their evergreen habit and well developed calyx lobes. The latter characters are shared by V. ovatum Pursh (V. sect. Pyxothamnus), the only other species of Vaccinium known from San Bruno Mountain, suggesting that the population is a hybrid between V. cespitosum and V. ovatum. We used data from gross morphology, leaf anatomy, and DNA sequences from the nuclear ribosomal internal transcribed spacer (ITS) and plastid matK and ndhF regions to test the hybrid status of this population. Hybrid status is supported by: 1) a plastid sequence profile identical to that of V. cespitosum from San Bruno Mountain and different from the profiles of all other Vaccinium samples in the study, 2) an ITS profile that differs from V. cespitosum from the mountain by only two polymorphic sites, and 3) the possession of gross morphological and anatomical characters that are either shared with one putative parent or the other, or are intermediate between them. Although ITS variation was non-additive and closely matched one of the putative parents, uniparental inheritance has been observed in other hybrids. Two novel gross morphological characters were also observed in the hybrid. The study documents a case of likely intersectional hybridization within Vaccinium, only rarely observed under natural conditions. Vaccinium cespitosum may have been outcompeted by the hybrid to the point of its extirpation from San Bruno Mountain. The population is here newly described as the nothospecies Vaccinium ×brunoense P.W.Fritsch, occurring in an area of only ca. 200 m2 with the number of individuals remaining uncertain because of clonal growth.

A Revised Phylogeny of the Mentha spicata Clade Reveals Cryptic Species.

The genus Mentha is taxonomically and phylogenetically challenging due to complex genomes, polyploidization and an extensive historical nomenclature, potentially hiding cryptic taxa. A straightforward interpretation of phylogenetic relationships within the section Mentha is further hindered by dominant but outdated concepts on historically identified hybrid taxa. Mentha spicata is traditionally considered to be of hybrid origin, but the evidence for this is weak. Here, we aim to understand the phylogenetic relationships within the section Mentha using large sample sizes and to revisit the hybrid status and identity of M. spicata. We show that two of three traditional species in the subsection Spicatae are polyphyletic, as is the subsection as a whole, while the real number of cryptic species was underestimated. Compared to previous studies we present a fundamentally different phylogeny, with a basal split between M. spicata s.s. and M. longifolia s.s. Cluster analyses of morphological and genotypic data demonstrate that there is a dissociation between morphologically and genotypically defined groups of samples. We did not find any evidence that M. spicata is of hybrid origin, and we conclude its taxonomic status should be revised. The combination of genetic and phenotypic information is essential when evaluating hyperdiverse taxonomic groups.