Relevance. Gender phytoregulators occupy an important place in the seed production of parental forms of cucumber, they allow you to shift the sex of the plant in the right direction, increase the intensity of selection, obtain purely female lines, and increase the efficiency of hybrid seed production.Results. The paper presents the regulations for the use of Esfon on the breeding line ZhL 24, which is the mother form of the hybrid Rich garden F1. To obtain the maximum number of female flowers, without significant inhibition of vegetative growth, it is necessary to treat vegetative plants three times with an interval of 5 days, starting from the phase of the second true leaf, with a solution of Esfon (BP. 65%) at a concentration of 0.03% (300 mg/l) with the purpose of preventing closely related crosses and increasing the hybridity of seeds. This technique stimulates the earlier development of pistillate flowers, minimizes the formation of male flowering, increases the number of fruits per plant, their size and weight, which allows increasing the yield per unit area by 40%.