Hybrid Varieties Research Articles

Identifying candidate genes for sugar accumulation in sugarcane: an integrative approach

BackgroundElucidating the intricacies of the sugarcane genome is essential for breeding superior cultivars. This economically important crop originates from hybridizations of highly polyploid Saccharum species. However, the large size (10 Gb), high degree of polyploidy, and aneuploidy of the sugarcane genome pose significant challenges to complete genome sequencing, assembly, and annotation. One successful strategy for identifying candidate genes linked to agronomic traits, particularly those associated with sugar accumulation, leverages synteny and potential collinearity with related species.ResultsIn this study, we explored synteny between sorghum and sugarcane. Genes from a sorghum Brix QTL were used to screen bacterial artificial chromosome (BAC) libraries from two Brazilian sugarcane varieties (IACSP93-3046 and SP80-3280). The entire region was successfully recovered, confirming synteny and collinearity between the species. Manual annotation identified 51 genes in the hybrid varieties that were subsequently confirmed to be present in Saccharum spontaneum. This study employed a multifaceted approach to identify candidate genes for sugar accumulation, including retrieving the genomic region of interest, performing a gene-by-gene analysis, analyzing RNA-seq data for internodes from Saccharumofficinarum and S. spontaneum accessions, constructing a coexpression network to examine the expression patterns of genes within the studied region and their neighbors, and finally identifying differentially expressed genes (DEGs).ConclusionsThis comprehensive approach led to the discovery of three candidate genes potentially involved in sugar accumulation: an ethylene-responsive transcription factor (ERF), an ABA 8’-hydroxylase, and a prolyl oligopeptidase (POP). These findings could be valuable for identifying additional candidate genes for other important agricultural traits and directly targeting candidate genes for further work in molecular breeding.

The characteristics and analysis of the complete chloroplast genome of Hemerocallis cultivar Small orange lamp 2019 (Asphodelaceae)

Hemerocallis cultivar Small orange lamp is a hybrid variety. Its whole chloroplast genome was 156,053 bp in size, consisting of 135 genes in total, including 89 mRNA genes, 38 tRNA genes, and 8 rRNA genes. The chloroplast genome contained a large single copy region (LSC 84,805 bp), a small single copy region (SSC 18,510 bp) and a pair of inverted repeats (IRa and IRb 26,369 bp). The overall GC content was 37.34%. Phylogenetic analysis showed that Hemerocallis lilioasphodelus and H. cultivar Small orange lamp are most closely related. This will provide valuable reference for further research on the genetic information of plants in the Hemerocallis.

Degradation of gluten using plant proteases

The paper describes the fact of the presence of a proteolytic enzyme in the leaves of the natural hybrid variety of grape - Isabella (Vitis Labrusca L.) and the perspective of its practical application for breaking down wheat gluten. As our research has shown, this enzyme effectively degradates the constituent components of the protein fraction of wheat flour-the gluten fraction. It is known that the wheat gluten is a strong allergen for many people, which can initiate a disease such as celiac disease. Protease from Isabella leaves carries out deep hydrolysis of gluten to low molecular weight soluble peptides and amino acids. This effect can be successfully used in the production of gluten-free bread products.

A Comparative Study on the Antioxidant Potentials of Hybrid and Generic Papayas Available in Jaffna, Sri Lanka

Generic papaya is a tropical plant abundant in Sri Lanka. Traditionally, it has been used therapeutically as it is a good source of polyphenols, carotenoids and an excellent source of vitamins that are powerful antioxidants. Specially, unripe fruit and leaf of papaya are recognized as a remedy for cancer and heart diseases due to their high antioxidant content. Recently, consumption of hybrid papaya fruits has increased as they are readily available in the local markets. However, the phytochemical constituents and quantity of these hybrid papaya varieties may differ from those available in the generic plant and hence their antioxidant potentials may also vary. Meanwhile there is concern within the community whether hybrid papaya has the same nutritional values as generic papaya. However, there have been no investigation done on the antioxidant activity of hybrid papaya available in Jaffna. Therefore, this study focused on investigating the in-vitro antioxidant potential of leaf and unripe fruit of generic and selected hybrid papaya varieties using 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. The unripe fruits and leaves of generic and selected hybrid (Red lady, Maradona, Tanin and Vega F1) varieties were collected from the Agricultural Research and Training Centre in Jaffna, Sri Lanka. The unripe fruit was sliced after removing the skin and seeds. The sliced unripe fruit and leaves were shade dried and powdered. The powdered plant materials were macerated with methanol and ethyl acetate separately for 48 hours at room temperature. The DPPH assay was carried out on the methanol and ethyl acetate extracts of the individual plant materials by employing ascorbic acid as the standard. The obtained data were analyzed using one-way ANOVA at 5% significance level and a significant difference between the antioxidant activities of the standard (IC50 value = 12.394 μg/mL) and the selected papaya extracts (IC50 values vary from 430.641 – 6,652.267 μg/mL) was found. Further, the selected hybrid papayas exhibited better antioxidant properties than the generic one; but, did not show a common trend within them. The leaf extracts were found to possess more antioxidant potential than the unripe fruit extracts of the same papaya variety. With respect to the solvents employed in extraction, methanolic extracts of the selected papayas showed better antioxidant activity than the corresponding ethyl acetate extracts. Preliminary phytochemical screening of the leaf and unripe fruit extracts of hybrid papayas revealed the presence of alkaloids, flavonoids, polyphenols and glycosides, which could be responsible for their antioxidant activity. The finding of the study revealed that hybrid papaya could be used as a raw material for functional food as generic papaya. However, there is a need to evaluate the other nutritional values to confirm it further.

Impact of Training Techniques and Hybrid Varieties on Tomato Yield in Shade Net House Cultivation

This study investigates the impact of training systems and hybrid selection on the yield of tomato (Solanum Lycopersicon) grown under a shade net house environment. The experiment, conducted at the Hi-Tech Horticulture Unit, Centre of Excellence for Vegetables, Karnal, utilized a Factorial Randomized Block Design with three replications. Two training systems (one-stem and two-stem) and four indeterminate hybrids (STH-39, STH-901, STH-801, and STH-701) were evaluated for their influence on key vegetative and reproductive parameters. Results revealed that the two-stem system significantly improved yield per plant (6.93 kg) and per square meter (15.36 kg) due to increased cluster formation and fruit set percentage. Conversely, the one-stem system promoted larger fruit size, with an average fruit weight of 105.50 g, indicating its suitability for markets valuing larger produce. Hybrid STH-801 exhibited superior performance, recording the highest yield per plant (6.52 kg) and yield per square meter (15.68 kg), along with the greatest number of fruits per cluster (7.75) and highest fruit set percentage (93.17%). Additionally, the STH-39 hybrid produced the largest fruits with an average diameter of 6.30 cm and volume of 157 cc. The findings underscore the importance of selecting appropriate training systems and hybrids to optimize productivity and fruit quality under protected cultivation. This research offers practical insights for growers seeking to enhance profitability through sustainable agricultural practices, particularly during off-season periods under challenging climatic conditions.

Survey and molecular characterization of begomovirus, and assessment of yield losses caused by leaf curl disease of sunflower (Helianthus annuus)

A comprehensive survey was conducted during winter (rabi) and rainy (kharif) seasons of 2019–20 to 2021–22 in the Kurnool, Nandyal, and Prakasam districts of Andhra Pradesh, focusing on the incidence of leaf curl disease in hybrid sunflower (Helianthus annuus L.) crop. The results revealed a high incidence of the disease, ranging from 40–94.5% across most of the surveyed hybrid varieties. Leaf curl-infected samples collected from the surveyed fields were analyzed by PCR using specific primers for the DNA-A component of the virus to confirm the presence of the pathogen. The PCR-amplified fragments were cloned and sequenced. Sequence analysis revealed that the sunflower isolate (Snf-AP) shared 99.2% nucleotide identity with the tomato leaf curl Karnataka virus (ToLCKV), which infects sunflower crops in Karnataka. This indicates a strong geographical and genetic connection between the viral strains affecting sunflowers in Andhra Pradesh and Karnataka. This genetic similarity is significant, as it suggests that the same or closely related viral strains are responsible for sunflower leaf curl disease across broader regions. The disease was found to affect sunflowers at all growth stages, with the highest incidence (42.3%) observed at the star bud stage. Infection at the star bud stage leads to substantial seed yield losses, with reductions of up to 82.8%. This level of damage underscores the economic impact of early infections, as yield losses of this magnitude can severely affect the profitability of sunflower farming. Additionally, a positive correlation was found between the whitefly population, weather parameters, and disease development.

Comparative Evaluation of Tomato Varieties: A Comprehensive Analysis of Yield Attributes

This article presents a comprehensive comparative evaluation of tomato varieties. The study focused on key yield attributes, including plant height, fruits per cluster, fruit diameter, fruit length, yield per hectare, days to 50% flowering, and maturity. Significant variations in plant height were observed among the varieties. Monoprecos had the tallest plant height (125.73 cm), while 2-HRDTOM035 was the shortest (83.26 cm). The number of fruits per cluster varied, with HRD109 producing the most (7.33) and HRDTOM084 the least (3.86). HRDTOM084 excelled in fruit dimensions, having the largest fruit diameter (19.88 mm) and fruit length (5.94 cm), whereas HRD109 showed the smallest diameter (10.16 mm). Yield per hectare also differed significantly, with HRDTOM084 delivering the highest yield (11.06 t/ha), and 2-HRDTOM035 recording the lowest (4.84 t/ha). The study also analyzed days to 50% flowering, revealing HRDTOM084 as the slowest to flower (48.60 days) and Monoprecos as the quickest (30.60 days). Days to maturity mirrored these results, with HRDTOM084 taking the longest to mature (93.73 days). In comparison, earlier studies with nine open-pollinated and hybrid varieties in other regions, such as Khajura and Lumle, revealed similar patterns in yield and growth, confirming the genetic diversity in tomato production. Overall, HRDTOM084 emerged as the most promising variety for commercial cultivation due to its superior yield, fruit size, and productivity, while Monoprecos and 2-HRDTOM035 may be suitable for environments that require shorter growth cycles. This review highlights the importance of selecting tomato varieties based on specific traits, such as plant height, yield potential, fruit quality, and adaptability to local growing conditions, to optimize production. Future research could focus on enhancing disease resistance and studying performance under different climatic conditions to further improve tomato cultivation practices in Nepal.

Analysis of inter-varietal hybridization in rice (Oryza sativa L.) indicate transgressive segregation

Traits under continuous stabilizing selection may yield transgressive phenotypes in suitable cross combinations. Farmers maintained Njavara (Oryza sativa L. landrace Njavara), a poor yielding medicinal rice landrace in Kerala state over centuries for medicinal applications using short duration (60-70 days) as a selection criterion. This implies that the heading date together with several of the yield related traits, which are co-controlled by pleiotropism, were under constant stabilizing selection in Njavara. We examined the level of transgression in 13 yield related traits in F2 populations raised from crosses between Njavara and a high yielding hybrid variety Jyothi. The cross yielded transgressive phenotype in all of the 13 traits in F2 populations. Difference in the genetic architecture between Njavara and Jyothi due to the difference in the selection pressure experienced by them may have contributed to the excessive transgression observed in the study. The results highlight the value of unattractive poor yielding land races in providing alleles to lift yield barrier in high yielders, and suggest the need to examine the genetic architecture of the trait of interest in parental lines for better genetic advancement in crop improvement programmes.

Comparative transcriptome analysis of maize (Zea mays L.) seedlings in response to copper stress.

Copper (Cu) is considered one of the major heavy metal pollutants in agriculture, leading to reductions in crop yield. To reveal the molecular mechanisms of resistance to copper stress in maize (Zea mays L.) seedlings, transcriptome analysis was conducted on the hybrid variety Zhengdan 958 exposed to 0 (control), 5, and 10 mM Cu stress using RNA-seq. In total, 619, 2,685, and 1,790 differentially expressed genes (DEGs) were identified compared to 5 mM versus 0 mM Cu, 10 mM versus 0 mM Cu, and 10 mM versus 5 mM Cu, respectively. Functional categorization of DEGs according to Gene Ontology revealed that heme binding, defense response, and multiorganism processes were significantly enriched under copper stress. Additionally, Kyoto Encyclopedia of Genes and Genomes enrichment analysis suggested that the copper stress response is mediated by pathways involving phenylpropanoid biosynthesis, flavonoid biosynthesis, and glutathione metabolism, among others. The transcriptome data demonstrated that metabolite biosynthesis and glutathione metabolism play key roles in the response of maize seedlings to copper stress, and these findings provide valuable information for enhancing copper resistance in maize.

Examination of the Effectiveness of Fruit Thinning in the Case of Idaho and Gala Must Early Apple Varieties

Abstract Apple (Malus domestica Borkh.) is the most important fruit in the EU. The total yield is more than 11 million tons.year-1, 15% of which is that of the early apple varieties. Several papers have already been published on the efficacy of crown and fruit thinning in the 20th century. Nowadays, hybrid varieties and intensive crown types are used in modernized systems. Our research was carried out in a Hungarian orchard during two cultivation periods. We studied two early apple varieties (Idaho, Gala Must) to determine how fruit thinning affected the quantity and quality of fruits. We found that the effectiveness of non-thinned technology is similar or slightly better compared to fruit thinning, after the second harvest. The average yield of Idaho apples per tree per harvest was 35.9 vs. 24.8 kg, while that of Gala Must was 50.8 vs. 59.2 kg (with thinning vs. without thinning). To assess quality, we measured water-soluble sugars, NO3 -, NO2 and vitamin C content. Immediately, after the harvest, a surprisingly high vitamin C content was found in the fruits (4.4 mg.100 g-1 on average), which, however, decomposed after 3 months of storage. We also determined the pathway of the decomposition of vitamin C.

Functional, Antioxidant, Antibacterial, and Antifungal Activity of Edible Flowers.

Edible flowers have been used since ancient times, but their potential for improving human health has not been explored. This study aimed to evaluate the profile of bioactive compounds (organic acids, phenolics, and carotenoids) and the antioxidant and antimicrobial activity of nine flower varieties with high concentrations of carotenoids or total phenolic compounds. Ninety-three edible flowers were analysed for physicochemical characteristics, total phenolic and carotenoid concentrations, and antioxidant activity (ABTS). Bioactive profiles were determined by rapid resolution liquid chromatography (RRLC), and antimicrobial activity was determined against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Streptococcus mutans, and Candida albicans and Candida tropicalis. Chrysanthemum x hybrid orange, Helianthus annuus yellow, Tagetes patula orange, Canna indica red, and Hibiscus rosa-sinensis (orange1 and yellow) showed significant concentrations of total carotenoids. In contrast, Pelargonium hortorum orange2, Hibiscus rosa-sinensis red1, and Rosa x hybrid variety medium yellow showed high levels of total phenolics. The predominant compounds in these species were citric acid (991.4 mg/g DW in Hibiscus rosa-sinensis red1), 4-hydroxybenzoic acid (936.2 mg/100 g DW in P. hortorum orange2), kaempferol (971. 9 mg/100 g DW in T. patula orange), quercetin glucoside (958.8 in C. x hybrid), quercetin (919.3 mg/100 g DW in T. patula), α-carotene, and β-carotene in T. patula orange (989.5 and 601.2 mg/100 g DW, respectively). Regarding antimicrobial activity, T. patula orange and P. hortorum orange2 inhibited bacterial growth, while C. x hybrid orange and P. hortorum orange2 inhibited Candida albicans, and the latter inhibited Candida tropicalis. These results indicate the potential of edible flowers as a natural source of bioactive compounds and as a tool in the fight against antimicrobial resistance.

Combining Ability of Capsicum annuum Hybrid for Antioxidant Activities, Polyphenol Content, α-Glucosidase Inhibitory, Yield, and Yield Components.

Chili (Capsicum annuum) consumption is often suggested, and using functional food cultivars is the most effective strategy post COVID-19 pandemic. Controlling chili breeding activity is one of the most effective methods to produce new hybrid varieties. However, the general combining ability (GCA), specific combining ability (SCA), and heterotic effect of functional biochemicals (polyphenol content, antioxidant activities, and α-glucosidase inhibitory compounds) remain poorly known in C. annuum. This study aimed to estimate these parameters in C. annuum by using five different genotypes and their hybrid combinations based on growth characteristics, yield, yield components, and fruit functional biochemicals. The F1 and F1R progenies were obtained from crosses in a greenhouse with a full diallel mating design. Each parent used in this study had a GCA advantage for each characteristic. The hybrid combination of IPB074 × IPB005 and IPB435 × IPB367 displayed the best yield results. However, the results indicated the opposite regarding α-glucosidase inhibitory compounds. The heterotic effect of functional biochemicals was observed for traits related to genotypes, polyphenol content, antioxidant activity, α-glucosidase inhibitory compounds, and similar properties related to yield and yield components, indicating their use in hybrid chili production.

Enhancing physio-biochemical characteristics in okra genotypes through seed priming with biogenic zinc oxide nanoparticles synthesized from halophytic plant extracts

Poor seedling germination and growth can result in large financial losses for farmers, thus, there is an urgent need for sustainable agricultural techniques to enhance seed germination and early growth. As an outcome, sustainable agriculture-which emphasizes the smart and effective utilization of resources-has gained popularity worldwide. At numerous levels, the field of nanotechnology is capable of significant benefit in achieving sustainable agricultural practices. Zinc oxide nanoparticles (ZnO NPs) have been shown to have biostimulatory properties and serve as effective solutions for addressing environmental and biotic stressors. The purpose of this study, investigating Salvadora persica halophytic leaf extract -synthesized zinc oxide nanoparticles (S-ZnONPs) as nano-priming agents to ensure okra seeds germinated under stress-free conditions. From an application perspective, we examined the effect of seed priming with varying concentrations of S-ZnO NPs (0, 20 and 40 ppm) for 18 and 24 h of soaking. Results indicated that the germination rate of hybrid variety improved with 20 ppm at 18 h, increasing by 58.22%, while mean germination time reduced by 24.62%. An enhancement trend was observed in the shoot, root length, shoot and root fresh weight, shoot and root dry weight of hybrid variety at 20ppm with 18 h priming by 34.2, 84.3, 80.2, 47.4, 50.3, and 36.2%, respectively. However, chlorophyll pigments chl a, chl b, and carotenoids was significantly raised in desi variety by 42.4, 79.31, and 142.29% with 20 ppm at 18 h priming. Hydrogen per oxide decreased up to 87.8% with 40 ppm at 24 h in hybrid variety, while, in desi variety H2O2 was reduced 88.3% with 20 ppm at 24 h. Non enzymatic antioxidant activities such as ascorbic acid, was highly increased 130.6% in hybrid at 24 h priming with 20 ppm dose. Flavonoids raised in same variety by 166.1% with 20 ppm at 18 h. Proline content was increased by 144.5% with 40ppm at 18 h. Moreover, Antioxidant enzymes, superoxide dismutase, peroxidase and catalase were significantly increased in both varieties with both levels of S-ZnO NPs and priming time. This cost-effective and environmentally safe technique to produce nanoparticles of different halophytic plants can maximize resource utilization, supporting sustainable agriculture by minimizing adverse environmental effects without compromising efficiency.

Comparison of residual heterosis in F2 and F3 segregating generations of Indian Mustard [Brassica juncea (L.) Czern & Coss.] crosses

Abstract The present investigation was undertaken to study the residual heterosis in F2 and F3 populations of Indian mustard [Brassica juncea (L.) Czern & Coss.]. The study material included eight parents and the 28 crosses of Indian mustard in two generations, namely F2 and F3. The experiment was conducted over two years at Uttar Banga Krishi Viswavidyalaya, Cooch Behar, West Bengal, during Rabi season 2020-21 and 2021-22. Out of the 28 crosses, three crosses namely, Npj-194 × DRMR-15-16, Npj-194 × RW- 85-59 (Sarma) and Npj-194 × SKJM-05 were found to show significantly positive residual standard heterosis over the standard variety (SKJM-05) for both the characters siliquae per plant and seed yield per plant. This provides us evidence that by improving siliquae per plant, the character seed yield per plant can be improved. The promising cross populations which have shown significant standard heterosis for seed yield in F2 and F3, can be utilized for the development of hybrid varieties, which would be very much beneficial to the marginal farmers of our country as they would be able to use the F2 and F3 seeds of the hybrid varieties without any compromise with seed yield. Keywords: Residual relative heterosis, Residual heterobeltiosis, Residual standard heterosis, Indian Mustard, Segregating generations

Molecular mechanisms of heterosis under drought stress in maize hybrids Zhengdan7137 and Zhengdan7153.

Maize is one of the most successful crops in utilizing heterosis which significantly improves maize stresses resistance and yield. Drought is a destructive abiotic stress that significantly reduces crop yield, particularly in maize. Drought stress and re-watering frequently occur during the growth and development of maize; however, the molecular mechanisms of heterosis under drought stress and re-watering have rarely been systematically investigated. Zhengdan7137 and Zhengdan7153 are two maize hybrid varieties with robust heterosis, and separately belongs to the SS×NSS and Reid×Tangsipingtou heterotic groups. 54 transcriptomes of these two hybrids and their parental inbred lines were analyzed under well-watering (WW), water-deficit (WD), and re-watering (RW) conditions using RNA-Seq. In this study, we identified 3,411 conserved drought response genes (CDRGs) and 3,133 conserved re-watering response genes (CRRGs) between Zhengdan7137 and Zhengdan7153. When comparing CDRGs and CRRGs to overdominance and underdominance genes, we identified 303 and 252 conservative drought response overdominance genes (DODGs) and underdominance genes (DUDGs), respectively, and 165 and 267 conservative re-watering response overdominance genes (RODGs) and underdominance genes (RUDGs), respectively. DODGs are involved in stress response-related processes, such as L-phenylalanine metabolism, carbohydrate metabolism, and heat response, whereas DUDGs are associated with glucose metabolism, pentose-phosphate shunt, and starch metabolism. RODGs and RUDGs contribute to the recovery of hybrids from drought stress by upregulating cell propagation and photosynthesis processes, and repressing stress response processes, respectively. It indicated overdominant and underdominant genes conservatively contributed to hybrid heterosis under drought stress. These results deepen our understanding of the molecular mechanisms of drought resistance, uncover conservative molecular mechanisms of heterosis under drought stress and re-watering, and provide potential targets for improving drought resistance in maize.

Micropropagation of Selected Hybrid Tomato (Solanum lycopersicum L.) Varieties Using Shoot Tip Culture

Tomato (Solanum lycopersicum L.) is one of the most important vegetable crops in the world. The use of hybrid varieties is a great option to increase its production but using F2 seed leads to lack of uniformity as a result of segregation. Therefore mass propagation using tissue culture could help to solve these problems. The objective of this study was to develop an optimum micropropagation protocol for Valouro, Uwezo and Shelter hybrid tomato varieties using shoot tip culture. Three successive experiments: shoot initiation; shoot multiplication and root inductions were conducted. Different concentrations of BAP (0.0, 0.25, 0.5, 0.75, 1.0 and 1.25 mg/l) and (0.0, 0.5, 1.0, 1.5, 2.0, 2.5 and 3 mg/l) were used for shoot initiation and shoot multiplication, respectively. While different concentrations of IBA (0.0, 0.25, 0.5, 0.75, 1.0, 1.25 and 1.5 mg/l) were used for root induction. The three separate experiments were laid out as factorial arranged in completely randomized design. The result of shoot initiation showed that the interaction of BAP*Varieties highly significant (P<0.01) effect. In the multiplication experiment, all parameters showed highly significant difference (P<0.01). In rooting experiment the interaction effect of IBA*Variety showed highly significant difference for the studied parameters. In conclusion MS medium without growth regulators was the optimum for shoot initiation. For shoot multiplication experiment 2mg/l, 0.5mg/l and 1.5mg/l of BAP was the optimum concentration for number of shoots per explant, shoot length, and leaf number respectively. For in vitro rooting MS medium containing 0.5 mg/l IBA was optimum for all varieties.

Network Topologies in Optical Systems

Abstract: Topology is the process of arranging objects in a certain connection, order, or configuration. Network topology, then, is the process of configuring or joining various devices inside a network. Network topologies are the various ways that devices can be connected to one another. Based on how devices are connected to one another and how data moves between them, these network topologies are categorized into several groups. System architecture uses a variety of topologies, including star, ring, hybrid, mesh, tree, and bus, in different sectors. This study has examined the advantages and disadvantages of several topologies based on connections and data flow. In this study, the significance of network topology will be discussed

Influence of Different Rates of Salinity on Flowering, Yield and Fruit Nutritional Value of Three Okra [<i>Abelmoschus esculentus </i>(L.) Moench] Cultivars in far North Region of Cameroon

<i>Context: </i>Salinity is in coastal, arid and semi-arid regions a major constraint in the productivity and agricultural development around the world. <i>Objectifs: </i>The objective of this study is to evaluate the effect of salinity on the growth, the nutritional value of the fruits of three okra (<i>Abelmoschus esculentus </i>L.) cultivars including two local (Javia and Parkwa) and a hybrid variety (Hire). <i>Methodology: </i>This is how four solutions of different NaCl concentrations from 0, 60, 120 to 240 mM were used to water okra plants at the four-leaves stage and this for two months in completely randomized device with four repetitions. <i>Results: </i>The results have differneces and similarities between the three varities during saline treatments. Salinity causes a decrease in growth, performance yield (from 0 to 240 mM NaCl to 28%, 23.6% and 22% in Parkwa, Hire, Javia cultivars respectively), mineral elements, antioxidants components and accumulation of Na content (to 45% in Parkwa, 23% in Hire and 18.4% in Javia from 0 to 240 mM NaCl) and flowering period (from 0 to 240 mM NaCl to 27.5%, 23.1% et 21.9% in Parkwa, Hire, Javia respectively). The reductions generated by salt have been less strong in Javia and Hire cultivars while the reductions were stronger at Parkwa cultivar. In addition, NaCl, at high concentrations, advantage of osmoticum accumulation involved in the osmotic ajustement mechanisms and would also serve as osmoprotector. Accumulation of osmolytes is salinity tolerance index that explains the maintenance of good water status in okra.<i> Conclusion:</i> Cultivars Javia and Hire were the most salt tolerant while the Parkwa was the most sensitive. The good behaviour of Javia and Hire varieties in the face of salinity can be considered for their use to better enhance the sahelian and coastal areas.

Cytoplasmic male sterility in plants with special emphasis on sugar beet

The phenomenon of cytoplasmic male sterility (CMS) in plants is characterized by a disturbance in the development of functional pollen. The cause of this disorder is the incompatibility of the mitochondrial and nuclear genome. Determinants of CMS are found in the mitochondrial genome and are inherited maternally. Nuclear Rf genes are responsible for restoring male fertility in CMS plants most often by posttranscriptional and/or posttranslational activity on mitochondrial gene expression. Two fertility restoring genes have been identified in sugar beet plants, of which Rf1 is the best characterized. It is concluded that the translation product of this gene acts as a molecular chaperone leading to inactivation of a specific mitochondrial protein that is a marker for the CMS trait in sugar beet. The CMS phenomenon is applied in the commercial breeding of hybrid varieties of many crop species. Exploiting heterosis is easier with the knowledge of cytoplasmic (mitochondrial) determinants of sterility and corresponding restorer genes.

THE INFLUENCE OF THE CONTENT OF TOTAL NITROGEN, POTASSIUM AND THE METHOD OF CULTIVATION ON SOME QUALITATIVE FEATURES OF STRAWBERRY

The qualitative composition of the ripe fruits of strawberry (hybrid variety- Alba) was examined on growing areas in Glumova (open field) and Shishova (in greenhouse conditions) in the municipality of Saraj- Skopje, with some different agrochemical characteristics, i.e. in Glumova the average total nitrogen values of 0.182% and potassium (K2O) 29.50mg/100g soil were significantly higher than those found in Shishova (0.116% and 20.24mg/100g soil). In ripe strawberry fruits, the amounts of total sugars, ascorbic acid (vitamin C), total nitrogen and potassium found were significantly dependent on the agrochemical properties and the method of their cultivation. In strawberry fruits grown in greenhouse conditions (Shishova), the amount of total sugars 3.68%, ascorbic acid (64.57 mg/100g FW), total nitrogen 9.10 mg/g-1 DM and potassium (K) 1.64 % in DM were significantly lower in relation to the average values found in strawberry samples grown in an open field (Glumova).