Cytoplasmic Hyaline Research Articles

THE PATHOLOGY OF CORTICOSTEROID-TREATED ASTHMA.

Abstract The following are the structural changes in cases of asthma that are related to long-term, high-dose corticosteroid therapy: (1) an external appearance of Cushing's disease, (2) a decrease in the inflammatory reaction, mucus gland activity, basement membrane prominence, and smooth muscle hypertrophy of the bronchial and bronchiolar walls, (3) a hypertrophy of the myocardial fibers, (4) an atrophy of the zona fasciculata of the adrenal cortices, (5) a cytoplasmic hyalinization and vacuolization of pituitary basophils and a hyperplasia of pituitary acidophils, (6) an atrophy of the lymphatic tissue of the spleen, and (7) a slight and infrequent osteoporosis (Table I). Long-term, high-dose corticosteroid therapy for asthma that begins after 40 years of age and is continuous is justified. The favorable antiinflammatory effect of corticosteroids on the bronchi and bronchioles probably accounts for the extended longevity. The other structural tissue alterations related to corticosteroids seem to be lesser evils when one is confronted with the consequences of this type of asthma.

A new factor associated with the implantation of the mouse egg.

of normal pregnancies. The pregnant uteri were obtained from females killed at intervals of 2 hr from 72 to 120 hr after timed matings. The uteri were fixed in aqueous Bouin, in Zenker or in Carnoy's fluid. Sections were cut in paraffin wax at 7 to 15 μ and stained with haematoxylin and eosin. Shortly after entering the uterus at about 72 hr post coitum, the blastocysts become randomly distributed throughout the uterine lumen. Between 84 and 96 hr post coitum they become antimesometrially situated in the lumen, then they lose their zona pellucidae and become orientated with their embryonic poles directed mesometrially. When the blastocysts reach their implantation sites, certain cells with a quite distinct morphology can be seen within the inner-cell-mass. The cells of the trophoblast and the inner-cell-mass generally have basophilic cytoplasm and large, oval, vesicular nuclei. Some cells within the inner-cell-mass, however, are seen to have eosinophilic, hyaline cytoplasm and rounded, dense, homo¬ geneous nuclei. These latter cells then apparently migrate from the inner-cell-

フークス科藻類の発生力学XVII.

Eggs of Coccophora Langsdorfii was ultracentrifuged just after fertilization at 25, 000 times gravity for five minutes. As a result, the following was discovered.(1) The intracellular materials are stratified into at least five layers. Arranged in order from the centripetal end, they are: the oil cap, the transparent layer of free water, the plastids and nucleus layer, the hyaline cytoplasm layer, and the granules layer.(2) When the centrifuged egg is immersed in distilled water, or in hypotonic sea water below 30per cent in concentration, the granules stratified in the centrifugal zone are darkened in color and the darkening begins at or near the centrifugal end and then spreads towards the centripetal side, then the plasmoptysis occurs in the centrifugal half. This indicates that the water permeability is highest at the centrifugal end.(3) When the centrifuged egg is immersed in the solution of the starfish toxin at or over 10-4 in concentration, the plasma membrane is destroyed in the subcentripetal zone surrounding the layer of hyaline cytoplasm. This indicates that a certain differentiation in susceptibility to starfish toxin has taken place in the plasma membrane or the cortical layer with the centrifugation. That is, the cortical layer including the plasma membrane is also movable by centrifuging, though the movement is invalid in determining the polarity axis.

OBSERVATIONS ON AMOEBOID MOTION OF LIVING HAEMOCYTES IN THE WING VEINS OF BLABERUS GIGANTEUS (L.) (ORTHOPTERA: BLATTIDAE)

Haemocytes with finely granular cytoplasm moved independently in partly occluded wing veins of B. giganteus by two related but fundamentally distinct methods: (a) typical amoeboid motion that occurred generally on unobstructed vein walls and was characterized by cytoplasmic streaming into amorphous pseudopodia, and (b) atypical amoeboid motion, without visible cytoplasmic streaming, that involved the projection of hyaline ectoplasm into tactile and adhesive filiform or lamellar pseudopodia. By the atypical method the cells became oriented and entered into confined spaces. Speed of movement varied but approximated 5 microns per minute with typical amoeboid motion and 3.5 with the atypical during pronounced cell migration. Haemocytes with hyaline cytoplasm, coarsely granular cytoplasm, or cytoplasm that contained numerous globules moved comparatively little and only by the atypical method.

A selective stain for cytoplasmic hyaline bodies in Laennec's cirrhosis.

Journal Article A Selective Stain for Cytoplasmic Hyaline Bodies in Laennec’s Cirrhosis* Get access Gert L. Laqueur, M.D. Gert L. Laqueur, M.D. From the Department of Pathology, Stanford University School of Medicine, San Francisco, California Search for other works by this author on: Oxford Academic Google Scholar American Journal of Clinical Pathology, Volume 20, Issue 7_ts, July 1950, Pages 689–690, https://doi.org/10.1093/ajcp/20.7_ts.689 Published: 01 July 1950 Article history Received: 11 March 1950 Published: 01 July 1950

Scarring and Precirrhosis of the Liver in Chronic Phosphorus Poisoning of Guinea Pigs

SummaryIn an attempt to produce portal cirrhosis, guinea pigs were fed white phosphorus in olive oil by mouth for periods up to 35 weeks. Microscopic examination of livers from animals killed after one week showed inconstant, slight to moderate cytoplasmic hyalinization and occasional to few necrotic liver cells. There was also moderate cellular infiltration of portal areas and parenchymal fatty metamorphosis; the latter was sometimes periportal in location. These changes continued to be observed in most animals throughout the experimental period with increasing amounts of collagen in portal areas appearing as the experiment progressed. The increase in collagen was definite but never great. In 4 animals thin collagenous trabecule connected portal areas and surrounded lobules. In a number of other animals fibrous extensions of portal areas were present but incomplete. There was neither prominent lobule distortion nor parenchymal hyperplasia. The failure to produce clear-cut portal cirrhosis is considered t...

ORIGIN AND FUNCTION OF THE PROTOPLASMIC CONSTITUENTS IN PELOMYXA CAROLINENSIS

1. Centrifugation causes the protoplasmic constituents of Pelomsyxa carolinensis to stratify into definite layers which, in order of decrease in weight, are as follows: refractive bodies, food-vacuoles, nuclei, crystals, beta granules, hyaloplasm, contractile vacuoles, and fat.2. The presence of at least one nucleus is necessary for normal locomotion and digestion, but not for ingestion of food in Pelomyxa. The length of life of Pelomyxa during starvation varies directly with the number of nuclei present.3. The contractile vacuoles arise de novo in the hyaline cytoplasm. Their formation and functioning is not dependent on the presence of nuclei or beta granules.4. The beta granules (mitochondria) do not play an active role in cell functions but, probably, merely give visible evidence of submicroscopic changes taking place in the hyaloplasm.5. The vacuole-refractive bodies and the crystals are formed from the food in the food-vacuoles of Pelomyxa and are used in the formation of the refractive bodies.6. The refractive bodies function as food reserve in the cell. Their complete removal does not impair any of the cellular activities.

Some structural transformations of the blood cells of vertebrates

The chief results of a study of structural transformations in the living blood cells of fishes, amphibians, reptiles, birds, and mammals are given in this paper.The blood cells were usually prepared for observation by mixing fresh blood with Ringer's fluid containing sufficient hirudin to prevent coagulation.Dark field illumination proved to be satisfactory for the observation of structural transformations in blood cells.The following five forms or phases may be assumed by vertebrate white blood cells:1. The hyaline surface phase which is characterized by a hyaline change and great increase in viscidity of the surface layer.2. The hyaline phase in which the globular component is localized in a small area of the extremely hyaline cytoplasm.3. The fine pseudopodial phase which is characterized by the protrusion of fine long motionless protoplasmic processes.4. The ciliated phase of the white blood cell shows numerous beating cilium-like processes.5. The flagellated phase is distinguished by the presence of ...