To study the influence of the Chinese high-speed railway ballastless track spectrum and the German low-interference spectrum on riding comfort, a long-span highway-railway suspension bridge is studied as an example, and a passenger–train–bridge coupling model is established based on the structural dynamics. The vibration responses of the passenger–train–bridge system during the CRH2C train running on the bridge are studied by a combination of the self-written computer program and general finite element software, considering the hunting movement, and the riding comfort of trains and passengers is evaluated using [Formula: see text] and ISO-2631 standard, respectively. The results show that track irregularity has a few effects on the lateral vibration of the bridge. Compared with the German low-interference spectrum, the Chinese high-speed railway ballastless track spectrum has less exciting effects on the vibration of the passenger–train–bridge system and better riding comfort. Passengers in the middle of the train and on the middle seats of the same train have better riding comfort, and with the increase of train speed, the riding comfort of passengers will gradually reduce. Instead of passenger responses, the riding comfort classification based on the car body is overestimated.