Hundreds Of Pores Research Articles

On the use of neural networks for the structural characterization of polymeric porous materials

The structural characterization is an essential task in the study of porous materials. To achieve reliable results, it requires to evaluate images with hundreds of pores. Current methods require large time amounts and are subjected to human errors and subjectivity. A completely automatic tool would not only speed up the process but also enhance its reliability and reproducibility. Therefore, the main objective of this article is the study of a deep-learning-based technique for the structural characterization of porous materials, through the use of a convolutional neural network. Several fine-tuned Mask R–CNN models are evaluated using different training configurations in four separate datasets each composed of numerous SEM images of diverse polymeric porous materials: closed-pore extruded polystyrene (XPS), polyurethane (PU), and poly(methyl methacrylate) (PMMA), and open-pore PU. Results prove the tool capable of providing very accurate results, equivalent to those achieved by time-consuming manual methods, in a matter of seconds.

Understanding the electronic pi-system of 2D covalent organic frameworks with Wannier functions

We investigate a family of hexagonal 2D covalent organic frameworks (COFs) with phenyl and biphenyl spacer units and different chemical linker species. Chemical trends are elucidated and attributed to microscopic properties of the pi-electron-system spanned by atomic p_z-orbitals. We systematically investigate the electronic structure, delocalization of electronic states, effects of disorder, bond torsion, and doping, and correlate these with variable pi-conjugation and nucleus-independent chemical shift (NICS) aromaticity. Molecular orbitals are obtained from maximally localized Wannier functions that have sigma- and pi-character, forming distinct sigma- and pi-bands for all valence states. The Wannier-orbital description goes beyond simple tight-binding models and enables a detailed understanding of the electronic topology, effective electronic coupling and delocalization. It is shown that a meaningful comparison between COFs with different chemical elements can only be made by examining the entire pi-electron system, while a comparison of individual bands (e.g., bands near the Fermi energy) can be a insufficient to derive general design rules for linker and spacer monomer selection. We further identify delocalized states that are spread across tens or hundreds of pores of the 2D COFs and analyze their robustness against structural and energetic disorders like out-of-plane rotations of molecular fragments, different strength of energetic disorder and energetic shifts due to chemical doping.

Tissue distribution of ethanol after intraprostatic injection using a porous needle.

To develop a safe and precise method for intraprostatic injection, and to establish correlation between the volume of ethanol injectate and the volume of subsequent infiltrated prostate tissue. We performed intraprostatic injection of 96% ethanol using a needle which has a segment of its wall made of capillary membrane with hundreds of pores in an acute and chronic canine experiment, in heart-beating cadaveric organ donors, and in a xenograft model of human prostate cancer. Whole mount tissue sections were used for three-dimensional reconstruction of the necrotic lesions and calculation of their volumes. The ethanol injection resulted in oval shaped lesions of well-delineated coagulative necrosis. In both healthy human and canine prostates, the prostatic pseudocapsule and neurovascular bundle remained intact without evidence of disruption. There was a linear correlation between administered volume of ethanol and the volume of necrotic lesion. Regression analysis showed strong correlation in the acute canine experiments and in experiments performed on xenografts of human prostate cancer. A formula was calculated for each experiment to estimate the relationship between the injected volume and the volume of infiltrated prostate tissue area. Intraprostatic injection using a porous needle allows for effective and predictable tissue distribution of the injectate in the prostate. Through varying the volume of the agent injected and use of needles with a different length of the porous segment, the volume of infiltrated tissue could be adjusted allowing for targeted focal treatment.

Mechanisms and kinetics of pore closure in thick aluminum plate

Interrupted in situ tests at high temperature characterized with X-ray microtomography were employed to study porosity evolution under loading conditions representative of aluminum hot rolling. Coupling digital volume correlation and simulations, it is possible to access, for each pore, its morphological evolution, and the mechanical fields it experienced. The closure kinetics of hundreds of pores were studied, which is described as the pore volume as a function of the hydrostatic integration. Closure kinetics are a function of the shape of the pore. Focus is put on pores with a complex shape which are more detrimental to mechanical properties. Their closure kinetics are exponential due to the pore fragmentation during closure. The closure of complex pores is fostered by an initial orientation perpendicular to the loading direction and a low initial fragmentation. Based on the closure kinetics of 243 complex pores, a new pore closure model is proposed.

An Electroactive Filter with Tunable Porosity Based on Glycolated Polythiophene

The porosity of filters is typically fixed; thus, complex purification processes require application of multiple specialized filters. In contrast, smart filters with controllable and tunable properties enable dynamic separation in a single setup. Herein, an electroactive filter with controllable pore size is demonstrated. The electroactive filter is based on a metal mesh coated with a polythiophene polymer with ethylene glycol sidechains (p(g3T2)) that exhibit unprecedented voltage‐driven volume changes. By optimizing the polymer coating on the mesh, controllable porosity during electrochemical addressing is achieved. The pores reversibly open and close, with a dynamic range of more than 95%, corresponding to over 30 μm change of pores’ widths. Furthermore, the pores’ widths could be defined by applied potential with a 10 μm resolution. From among hundreds of pores from different samples, about 90% of the pores could be closed completely, while only less than 1% are inactive. Finally, the electroactive filter is used to control the flow of a dye, highlighting the potential for flow control and smart filtration applications.

Reconstitution of Ultrawide DNA Origami Pores in Liposomes for Transmembrane Transport of Macromolecules.

Molecular traffic across lipid membranes is a vital process in cell biology that involves specialized biological pores with a great variety of pore diameters, from fractions of a nanometer to >30 nm. Creating artificial membrane pores covering similar size and complexity will aid the understanding of transmembrane molecular transport in cells, while artificial pores are also a necessary ingredient for synthetic cells. Here, we report the construction of DNA origami nanopores that have an inner diameter as large as 30 nm. We developed methods to successfully insert these ultrawide pores into the lipid membrane of giant unilamellar vesicles (GUVs) by administering the pores concomitantly with vesicle formation in an inverted-emulsion cDICE technique. The reconstituted pores permit the transmembrane diffusion of large macromolecules, such as folded proteins, which demonstrates the formation of large membrane-spanning open pores. The pores are size selective, as dextran molecules with a diameter up to 28 nm can traverse the pores, whereas larger dextran molecules are blocked. By FRAP measurements and modeling of the GFP influx rate, we find that up to hundreds of pores can be functionally reconstituted into a single GUV. Our technique bears great potential for applications across different fields from biomimetics, to synthetic biology, to drug delivery.

Multi-pore carbon phase plate for phase-contrast transmission electron microscopy.

A new fabrication method of carbon based phase plates for phase-contrast transmission electron microscopy is presented. This method utilizes colloidal masks to produce pores as well as disks on thin carbon membranes for phase modulation. Since no serial process is involved, carbon phase plate membranes containing hundreds of pores can be mass-produced on a large scale, which allows "disposal" of contaminated or degraded phase modulating objects after use. Due to the spherical shape of the mask colloid particles, the produced pores are perfectly circular. The pore size and distribution can be easily tuned by the mask colloid size and deposition condition. By using the stencil method, disk type phase plates can also be fabricated on a pore type phase plate. Both pore and disk type phase plates were tested by measuring amorphous samples and confirmed to convert the sinus phase contrast transfer function to the cosine shape.

Morphology, Genesis, and Distribution of Nanometer-Scale Pores in Siliceous Mudstones of the Mississippian Barnett Shale

Research on mudrock attributes has increased dramatically since shale-gas systems have become commercial hydrocarbon production targets. One of the most significant research questions now being asked focuses on the nature of the pore system in these mudrocks. Our work on siliceous mudstones from the Mississippian Barnett Shale of the Fort Worth Basin, Texas, shows that the pores in these rocks are dominantly nanometer in scale (nanopores). We used scanning electron microscopy to characterize Barnett pores from a number of cores and have imaged pores as small as 5 nm. Key to our success in imaging these nanopores is the use of Ar-ion-beam milling; this methodology provides flat surfaces that lack topography related to differential hardness and are fundamental for high-magnification imaging. Nanopores are observed in three main modes of occurrence. Most pores are found in grains of organic matter as intraparticle pores; many of these grains contain hundreds of pores. Intraparticle organic nanopores most commonly have irregular, bubblelike, elliptical cross sections and range between 5 and 750 nm with the median nanopore size for all grains being approximately 100 nm. Internal porosities of up to 20.2% have been measured for whole grains of organic matter based on point-count data from scanning electron microscopy analysis. These nanopores in the organic matter are the predominant pore type in the Barnett mudstones and they are related to thermal maturation. Nanopores are also found in bedding-parallel, wispy, organic-rich laminae as intraparticle pores in organic grains and as interparticle pores between organic matter, but this mode is not common. Although less abundant, nanopores are also locally present in fine-grained matrix areas unassociated with organic matter and as nano- to microintercrystalline pores in pyrite framboids. Intraparticle organic nanopores and pyrite-framboid intercrystalline pores contribute to gas storage in Barnett mudstones. We postulate that permeability pathways within the Barnett mudstones are along bedding-parallel layers of organic matter or a mesh network of organic matter flakes because this material contains the most pores.

The three-dimensional architecture of collagen fibrils in the lamina cribrosa and the anterior part of the optic nerve

The three-dimensional architecture of the collagen fibrillar network forming the lamina cribrosa of the optic disc and the associated septal plates of the anterior part of the optic nerve was investigated using monkey eyeballs treated by the NaOH cell-maceration procedure and scanning electron microscopy. Arising from the scleral collagen fibrils branches joined together to form thick bundles of collagen in lamina plates encompassing hundreds of pores. More than ten plates were observed stacked on top of each other forming the pore channels. Adjacent piled up plates were interconnected with branching collagen fibrils. Septal plates derived from pia mater collagen fibrils were formed by radially and obliquely arranged thick bundles of collagen piled up longitudinally in the optic nerve and merging into the most posterior lamina plate. In the septal plates many ovally shaped windows were observed, suggesting branching and regional irregularity in the shape of the myelinated nerve fiber bundle. Pia mater and dura mater collagen fibrils of the optic nerve merged into the outer layer of the scleral collagen fibris. The present results suggest that the observed three-dimensional network of collagen fibrils constitutes the skeletal framework of these tissues maintaining their morphological specificity and providing a suitable microenvironment for their physiological functions.

Theory of mobilization pressure gradient of flowing foams in porous media: I. Incompressible foam

A theory is presented for the minimum pressure gradient (∇ p) min required to keep a foam flowing through a porous medium. The theory accounts for pore shape, foam texture, contact-angle hysteresis, and bubble separation at pore throats, but not for the effects of gas compressibility or of the Plateau borders where liquid lamellae contact the pore wall. Quantitative estimates of (∇ p) min depend on pore shape, foam texture, and surface tension. For medium-textured foams (bubbles 400 μm in diameter in bulk), reasonable parameter values give (∇ p) min = 18–32 kPa/m (0.8–1.4 psi/ft) for CO 2 foams and 125–240 kPa/m (5–11 psi/ft) for others foams. Lower gas/liquid surface tension causes the lower estimate for CO 2 foams. For most foams, foam texture must coarsen substantially as foam flows away from the well in oilfield application, or formation plugging results. Further, to flow at 20–45 kPa/m (1–2 psi/ft), foam bubbles must be at least 1 or more cm, or hundreds of pores, in length. CO 2 foams, on the other hand, can still flow if bubbles are somewhat smaller. The highest values of (∇ p) min are predicted for foams in pores with high aspect ratio (body radius/throat radius), sharp corners, and narrow throats, and at low capillary pressures (wet foams).

Cytoskeletal reorganization and plasma membrane fusion in conjugating Tetrahymena.

The conjugation junction of Tetrahymena is the specialized site where plasma membrane fusion occurs between two cells of complementary mating types. The junction is constructed through a series of cooperative interactions and morphogenetic steps. A contact-mediated interaction between free-swimming, sexually mature and mating-competent cells of two complementary mating types induces a morphological transformation of the anterior tips. Cells then join in pairs aligned by the apposition of their modified tips. Thin sections show that the plasma membranes of the tips are separated by approximately 500 A of extracellular space, in which some strands of matrix material can be identified. The cytoplasmic face of the membrane is in contact with a junction-specific thick layer of electron-dense material. At hundreds of independent sites in this junction plasma membranes fuse in a limited manner, thereby establishing hundreds of separate membrane-ensheathed cytoplasmic channels that connect the two cells. At the same locations the thick submembrane layer is interrupted. Consequently, the junction appears to be a structure that is perforated with hundreds of pores. This study poses the question of whether the junction's submembrane layer is, or includes, a skeletal element. Cells were extracted with the non-ionic detergent Triton X-100 under conditions that yield cytoskeletal frameworks (CFs) that maintain the morphological integrity of the cells. The CFs include chromatin and also cortical structures such as microtubule bands, basal bodies, ciliary axonemes, kinetodesmal fibres and fibrillar epiplasm. CFs of conjugant pairs are also paired, indicating that the junction contains a skeletal element that is responsible for integrating the individual CFs into a higher-order complex. At the ultrastructural level the skeletal structure of the junction includes membrane lamina and a submembrane scaffold, residues of the plasma membrane and thick submembrane layer, respectively, both of which are interrupted at the pores. However, the two separate scaffolds are joined at the rims of the pores. This provides a means by which the separate CFs become integrated. On the basis of images of junctional CFs, which show interruptions of the scaffold without concomitant membrane fusion, but where laminae are pressed close together, a specific model of membrane fusion is proposed. According to this model, the submembrane skeletal scaffold regulates membrane fusion by limiting its occurrence, and the extent of its occurrence.

Two-Phase Flow in Random Network Models of Porous Media

Abstract To explore how the microscopic geometry of a pore space affects the macroscopic characteristics of fluid flow in porous media, we have used approximate solutions of the Navier-Stokes equations to calculate the flow of two fluids in random networks. The model pore space consists of an array of pores of variable radius connected to a random number of nearest neighbors by throats of variable length and radius. The various size and connectedness distributions may be arbitrarily assigned, as are the wetting characteristics of the two fluids in the pore space. The fluids are assumed to be incompressible, immiscible, Newtonian, and of equal viscosity. In the calculation, we use Stokes flow results for the motion of the individual fluids and incorporate microscopic capillary force by using the Washburn approximation. At any time, the problem is mathematically identical to a random electrical network of resistors, batteries, and diodes. From the numerical solution of the latter, we compute the fluid velocities and saturation rates of change and use a discrete timestepping procedure to follow the subsequent motion. The scale of the computation has restricted us so far to networks of hundreds of pores in two dimensions (2D). Within these limitations, we discuss the dependence of residual oil saturations and interface shapes on network geometry and flow conditions.