Abstract This study examined the reliability, validity, and factor structure of the sense of humor scale (SHS; McGhee, Paul E. 1999. Health, healing and the amuse system: Humor as survival training, 3rd edn. Dubuque: Kendall/Hunt), a 24-item questionnaire developed to measure overall sense of humor. Participants included 99 adults, 105 undergraduates, and 111 comedians. One-week test-retest reliability was good (r = 0.75). Internal consistency of the overall scale was excellent, and acceptable-to-excellent for the six subscales. Item-total correlations were generally strong. Comedians scored higher than undergraduates and adults, supporting the construct validity of the SHS. Convergent validity was strong as the SHS was positively correlated with the Humor Styles Questionnaire total and its subscales. Our analyses of SHS’s associations with the Big Five personality dimensions led to findings that are consistent with prior research, as the SHS was positively correlated with extraversion and openness to experience, but uncorrelated with neuroticism, agreeableness, and conscientiousness. Factor analyses found a bifactor model to be the best fitting model for the SHS. Ancillary bifactor fit indices provided additional support for the notion that the SHS may not be best described as unidimensional. Thus, it can be argued that the subscales are relevant for both research and applied work as they offer unique contributions.