The stability of loess subgrade is affected by many factors. Dry-wet cycle is a common factor that causes damage to the strength of loess subgrade. In order to study the influence of dry-wet cycle on the stability of loess subgrade, reveal the mechanism of dry-wet cycle damage to the strength of loess subgrade It is necessary to sample the loess soil samples in this area, and perform compaction test, dry-wet cycle test, unconfined compression test and scanning electron microscope test on the soil sample to analyze the influence of dry-wet cycle on the strength and microstructure of loess soil. . In this way, the potential relationship between the microstructure and the strength of the soil can be obtained. The results show that the loess drywet cycle causes the loess's tensile strength and compressive strength to decrease with the increase of the number of dry-wet cycles. The dry-wet cycle effect gradually reduces the shear structural strength of the undisturbed loess and eventually disappears. Scanning electron microscope tests found that the humidification process caused slight movement of the particles, while the drying process resulted in permanent deformation of small pores.