Rapid urbanization increases the densely built-up blocks, the population and vehicles. Large amounts of particulate matter (PM), especially PM2.5 (PM with an aerodynamic diameter of 2.5μm or less), from vehicle exhaust are critical to human health. In typical street canyons in hot and humid regions, traffic-source PM usually diffuses to the densely built-up blocks through roadside trees. Roadside trees are a double-edged sword, serving as “guards” to absorb PM2.5 while may lead to PM2.5 gathering in street levels, thereby influencing the PM2.5 dispersion in the densely built-up blocks. To quantify the dispersion process, this study proposed traffic-source PM2.5 dynamic dispersion models considering the capture capability of roadside trees and built-up blocks based on the OSPM model. Due to the difficulty in obtaining the adsorption and deposition rate of the proposed models, the numerical simulations by ENVI-met software were used to solve and obtain the relationship between capture capability and characteristic index of roadside trees. Subsequently, The accuracy and effectiveness of the proposed traffic-source PM2.5 dynamic dispersion models were verified through field experimental data. Results show that the calculated PM2.5 concentration significantly linearly increased with the measured values with the determined coefficient (R2) of 0.98, and the first-order coefficient close to 1. It indicates that the proposed traffic-source PM2.5 dispersion model accurately quantified the impact of roadside trees on PM2.5 and its concentration dispersion process to the built-up blocks. This study provides suggestions for designing characteristic indexes of roadside trees and built-up blocks to improve the air quality of urban street canyons.