Summary This investigation in the humid-tropical mountains of New Guinea is part of a series of comparative studies about the altitudinal variation of physiological and anatomical leaf characteristics in different mountain regions of the world. The microscopic anatomical features and the diffusive conductance for water vapour in leaves of sclerophyll shrubs, dwarf shrubs, tussock grasses and herbs were studied in open habitats between 1,100 and 4,420 m altitude. Particular attention was directed to the Ericaceae family and the genera Rhododendron, Vaccinium and Gaultheria. The field studies were supplemented by simultaneous measurements of leaf and air temperature, air humidity and photon flux area density. The following results were obtained: As in altitudinal gradients in other mountains, plant size, leaf size and specific leaf area tend to decrease with increasing elevation. However, in contrast to the results of previous studies, maximum leaf conductance decreases with altitude in New Guinea. The diurnal variation of leaf conductance decreases. Stomatal frequency and particularly the ratio between the number of stomata and the total number of epidermal cells also decrease. The size of stomata does not change, as cell size in both epidermis and mesophyll does not reveal any significant change. The thickness of cell walls generally increases. This is most pronounced in the epidermis. Also cuticula thickness increases with altitude, but this does not lead to a lowered minimum leaf conductance. Comparing these results with those from other mountain areas it can be concluded that the leaf characteristics of plants from high altitudes can be divided at least in two groups: 1. Characteristics like size and scleromorphy of leaves, which tend to change in a similar manner in all mesic or humid mountain areas, more or less independently of the specific local climate, and are probably determined by direct or indirect effects of the mean temperature during leaf growth. 2. Characteristics whose altitudinal change is determined by climatic elements which are not generally coupled with altitude as for instance the dosis of photosynthetic active radiation received by a leaf (which drastically decreases with altitude in New Guinea). Maximum leaf conductance seems to be controlled largely by those factors. Temperature and partial pressure of CO 2 both declining similarly in all mountains, do not seem to be chief determinants for modification of leaf conductance with altitude. The study provides experimental evidence that similar altitudinal changes in the physiognomy of plants in mountain areas of different regions are not necessarily accompanied by similar physiological and anatomical alterations in the leaves.
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