Human Environment Research Articles

Integrated transcriptomic and metabolomic analysis reveals the underlying mechanisms for male reproductive toxicity of polystyrene nanoplastics in mouse spermatocyte-derived GC-2spd(ts) cells

BackgroundPolystyrene nanoplastics (PS-NPs), are ubiquitous pollution sources in human environments, posing significant biosafety and health risks. While recent studies, including our own, have illustrated that PS-NPs can breach the blood-testis barrier and impact germ cells, there remains a gap in understanding their effects on specific spermatogenic cells such as spermatocytes. Methods and resultsHerein, we employed an integrated approach encompassing phenotype, metabolomics, and transcriptomics analyses to assess the molecular impact of PS-NPs on mouse spermatocyte-derived GC-2spd(ts) cells. Optimal exposure conditions were determined as 24 h with 50 nm PS-NPs at 12.5 μg/mL and 90 nm PS-NPs at 50 μg/mL for subsequent multi-omics analysis. Our findings revealed that PS-NPs significantly influenced proliferation and viability, causing alterations in transcriptome and metabolome profiles. Transcriptomics analysis of GC-2spd(ts) cells exposed to PS-NPs indicated the pivotal involvement of cell proliferation and cycle, autophagy, ferroptosis, and redox reaction pathways in PS-NP-induced effects on the proliferation and viability of GC-2spd(ts) cells. Furthermore, metabolomics analysis identified major changes in amino acid metabolism, cyanoamino acid metabolism, and purine and pyrimidine metabolism following PS-NP exposure. ConclusionOur integrated approach, combining metabolomics and transcriptomics profiles with phenotype data, enhances our understanding of the adverse effects of PS-NPs on germ cells.

Emerging Microorganisms and Infectious Diseases: One Health Approach for Health Shared Vision.

Emerging infectious diseases (EIDs) are newly emerging and reemerging infectious diseases. The National Institute of Allergy and Infectious Diseases identifies the following as emerging infectious diseases: SARS, MERS, COVID-19, influenza, fungal diseases, plague, schistosomiasis, smallpox, tick-borne diseases, and West Nile fever. The factors that should be taken into consideration are the genetic adaptation of microbial agents and the characteristics of the human host or environment. The new approach to identifying new possible pathogens will have to go through the One Health approach and omics integration data, which are capable of identifying high-priority microorganisms in a short period of time. New bioinformatics technologies enable global integration and sharing of surveillance data for rapid public health decision-making to detect and prevent epidemics and pandemics, ensuring timely response and effective prevention measures. Machine learning tools are being more frequently utilized in the realm of infectious diseases to predict sepsis in patients, diagnose infectious diseases early, and forecast the effectiveness of treatment or the appropriate choice of antibiotic regimen based on clinical data. We will discuss emerging microorganisms, omics techniques applied to infectious diseases, new computational solutions to evaluate biomarkers, and innovative tools that are useful for integrating omics data and electronic medical records data for the clinical management of emerging infectious diseases.

A smartphone-assisted portable on-site detection system for organophosphorus pesticides in vegetables and fruits based on all-in-one paper-based sensors: 2,2-Dichlorovinyl dimethyl phosphate as a model

The improper use of organophosphate pesticides (OPs) can lead to residue posing a serious threat to human health and environment. Therefore, the development of a simple, portable, and sensitive detection method is crucial. Herein, a bioenzyme-nanozyme-chromogen all-in-one paper-based sensor was synthesized. Initially, the Ce/Zr-MOF with peroxidase-like activity was grown on filter paper (FP) using in-situ solvent thermal method, resulting in Ce/Zr-MOF@FP. Subsequently, the AChE-ChO-TMB system was immobilized onto Ce/Zr-MOF@FP using biocompatible gelatin, which enhanced cascade catalysis efficiency through the proximity effect. Based on the inhibition principle of OPs on AChE, we integrated this sensor with Python-based image recognition algorithm to achieve detection of OPs. Using 2,2-dichlorovinyl dimethyl phosphate (DDVP) as a model of OPs, it has good detection performance with a detection limit of 0.32 ng mL−1 and a recovery rate range of 95–107%. The potential for on-site detection of DDVP residues in vegetables and fruit samples is highly promising.

Authentication of sushi and sashimi products from northeastern Pará, coastal Amazon, by DNA barcoding reveals commercial frauds and potential threats to human health and environment

The Japanese cuisine has become a huge success in Brazil over the last years, but little is known about the fisheries resources commercialized to supply this specific market. In the present study, we carried out the first molecular characterization of sushi and sashimi products available in northeastern Pará, coastal Amazon, in order to infer what species have been actually exploited in this trade. We sequenced two mitochondrial DNA regions from 172 samples (138 of fish and 34 of shrimp-derived products) that were divided into 28 haplotypes. The comparative analyses with public DNA barcode databases revealed eight species of fish and three of shrimps among the samples, including cases of commercial frauds. For instance, Ruvettus pretiosus and Lepidocybium flavobrunneum were traded as “anchova” (bluefish), a valid and specific commercial name of Pomatomus saltatrix. Both species can cause gastrointestinal issues, which has led to restrictions on their sale in several countries. All commercialized shrimp-derived products were labeled as “camarão” (shrimp), as generic trade name that could not be applied to identify each species. Furthermore, potentially threatened species have also been recorded in the commercial products, such as Salmo salar and Cynoscion acoupa, classified as “nearly threatened” and “vulnerable”, respectively. The later, popularly known as “pescada-amarela” (acoupa weakfish) has been commercialized as “peixe-branco” (white fish), a designation that is not recognized by the Brazilian regulatory agencies. Both DNA markers (COI and 16S rRNA) were highly efficient in identifying the species from all samples, providing the first list of exploited species for Japanese cuisine along the coastal Amazon. Besides revealing issues in the commercialization of Japanese food items, we recorded a largely overlooked diversity of traded species. These data represent a potential risk to public health and to the environmental conservation such as the presence of exotic species and the exploitation of threatened species that should be considered to support effective policies for the regional regulation of this market.

Research on Artificial-Intelligence-Assisted Medicine: A Survey on Medical Artificial Intelligence.

With the improvement of economic conditions and the increase in living standards, people's attention in regard to health is also continuously increasing. They are beginning to place their hopes on machines, expecting artificial intelligence (AI) to provide a more humanized medical environment and personalized services, thus greatly expanding the supply and bridging the gap between resource supply and demand. With the development of IoT technology, the arrival of the 5G and 6G communication era, and the enhancement of computing capabilities in particular, the development and application of AI-assisted healthcare have been further promoted. Currently, research on and the application of artificial intelligence in the field of medical assistance are continuously deepening and expanding. AI holds immense economic value and has many potential applications in regard to medical institutions, patients, and healthcare professionals. It has the ability to enhance medical efficiency, reduce healthcare costs, improve the quality of healthcare services, and provide a more intelligent and humanized service experience for healthcare professionals and patients. This study elaborates on AI development history and development timelines in the medical field, types of AI technologies in healthcare informatics, the application of AI in the medical field, and opportunities and challenges of AI in the field of medicine. The combination of healthcare and artificial intelligence has a profound impact on human life, improving human health levels and quality of life and changing human lifestyles.

Efecto del uso de boquillas de aire inducido y adyuvante sobre la calidad de aplicación de fitosanitarios

Spraying of phytosanitary products must ensure efficient pest control while avoiding losses that could damage human health and environment. The objective of the study was to evaluate the effect of conventional hollow cone and air-induced hydraulic nozzles, and the addition of an adjuvant (Methylated Seed Oil [MSO]) upon the application quality of a fungicide (penetration, distribution, and uniformity) and losses due to endodrift on wheat and soybean canopies. In each crop, the experimental design was completely randomized,with four replications and a factorial arrangement of nozzles x adjuvant. Droplet spectrum was evaluated by using water-sensitive cards, and brilliant blue as a tracer in the application mixture. According to the results, the MSO did not homogenize the distribution of droplets generated by air-induced nozzles in the wheat canopy, as it depended on the crop height evaluated. In addition, the MSO reduced endodrift independently of the type of nozzle used. Thedistribution of droplets on the soybean canopy depended on the height and not on the type of nozzle nor the addition of MSO. The MSO and its combination with the nozzle type did not reduce endodrift in this crop.

Fabrication of ternary Bi2S3/BiVO4/g-C3N4 composite material for enhanced photocatalytic degradation of antibiotics

Antibiotic contaminants are known to pose a serious threat to the human health and environment, thus a great deal of effort has been made to develop destruction technologies for antibiotic treatment in the water environment. Photocatalytic oxidation technology is regarded as one of the most promising strategies for decomposing antibiotics from water. In this work, ternary Bi2S3/BiVO4/g-C3N4 composite material is dexterously designed and synthesized to obtain the enhanced photocatalytic performance for tetracycline (TC) degradation via a facile strategy. Therein, the optimal Bi2S3/BiVO4/g-C3N4 material showed the excellent degradation efficiency under visible light irradiation in comparison to the nude g-C3N4 and BiVO4/g-C3N4 photocatalysts, and it can continuously degrade the TC pollutant in 90 min and achieve a degradation percentage of 80.7 %. The designed materials can not only intensify photo-absorption efficiency of g-C3N4 material, but also improve its separation and transfer efficiency of photo-generated charge carriers, which is attributed to the incorporation of BiVO4 and Bi2S3 materials. The active species trapping experiments verified that the superoxide radicals (•O2−) is the main active species, and holes (h+) also played an important role in the Bi2S3/BiVO4/g-C3N4 system. Furthermore, the photoelectrochemical measurement (PEC) clearly demonstrated that the incorporation of BiVO4 and Bi2S3 materials could accelerate the separation and transfer of photo-generated electron and hole pairs. As expected, our present work could spark new inspirations to further develop the efficient photocatalysts for solar energy conversion.

Natural Products of Licorice for Uranium Decorporation with Low Toxicity and High Efficiency.

The development and exploration of uranium decorporation agents with straightforward synthesis, high removal ability, and low toxicity are crucial guarantees for the safety of workers in the nuclear industry and the public. Herein, we report the use of traditional Chinese medicine licorice for uranium decorporation. Licorice has good adsorption performance and excellent selectivity for uranium in the simulated human environment. Glycyrrhizic acid (GL) has a high affinity for uranium (p(UO2) = 13.67) and will complex with uranium at the carbonyl site. Both licorice and GL exhibit lower cytotoxicity compared to the commercial clinical decorporation agent diethylenetriamine pentaacetate sodium salts (CaNa3-DTPA). Notably, at the cellular level, the uranium removal efficiency of GL is eight times higher than that of CaNa3-DTPA. Administration of GL by prophylactic intraperitoneal injection demonstrates that its uranium removal efficiency from kidneys and bones is 55.2 and 23.9%, while CaNa3-DTPA shows an insignificant effect. The density functional theory calculation of the bonding energy between GL and uranium demonstrates that GL exhibits a higher binding affinity (-2.01 vs -1.15 eV) to uranium compared to DTPA. These findings support the potential of licorice and its active ingredient, GL, as promising candidates for uranium decorporation agents.

Direct Air Capture (DAC) for Achieving Net-Zero CO2 Emissions: Advances, Applications, and Challenges

Carbon dioxide (CO2), as the primary greenhouse gas, has significant impacts on global climate change, leading to severe and irreversible adverse consequences for ecosystems and human environments. To address the issue of excessive CO2 emissions, efforts in recent years have yielded significant progress in the development of clean energy sources and the promotion of carbon capture, utilization, and storage (CCUS) technologies. Conventional CO2 capture techniques are limited in addressing global atmospheric CO2 excess effectively, as they target only high-concentration CO2 emissions and require implementation at specific emission points. Direct air capture (DAC) technology has emerged as a promising solution due to its flexibility in deployment, avoidance of land competition, and ability to capture legacy CO2 emissions. Additionally, DAC offers opportunities for producing synthetic clean fuels, thereby reducing reliance on traditional fossil fuels and aiding in reducing greenhouse gas emissions. This study provides a comprehensive review of DAC technology, encompassing its principles, technological advancements, real-world applications, challenges, and future research directions. By offering insights into the current state and potential of DAC technology, this study aims to guide global efforts in scaling up DAC deployment, ultimately contributing to achieving global carbon neutrality or even negative emissions.

How Do the Home Country Regulations Promote the Responsibility for Overseas Farmland Investment?

Restricted by economic and social development conditions and the level of domestic regulatory system construction, the traditional host country-centered regulatory approach makes it difficult to ensure “responsible investment” by overseas farmland investment companies. The jury is still out on whether international law can directly impose obligations and responsibilities on companies. Therefore, it is realistically necessary for the investor’s home country to regulate any behavior that infringes on the human rights and environment of residents of the host country during the company’s overseas farmland investment. We used a panel fixed effects model and the dummy variable method to construct regression indicators based on international treaties. This study finds that promulgating responsible investment treaties can significantly promote the scale and number of international farmland investments, which also further led to the problem of differentiation among overseas farmland investors. We further discussed the lack of binding force of current international rules and the difficulty in coordinating the interests of multiple parties. In the regulatory system of the home country, there are still problems, such as insufficient supply of adequate laws, and lack of administrative guidance. Measures such as deepening negotiations on international rules for responsible overseas farmland investment, further leveraging the role of land ownership in responsible overseas farmland investment, improving existing domestic legislation, and strengthening administrative guidance on overseas farmland investment can be used as countermeasures.

P-241 The in vivo human uterine pH and uterine fluid composition differs from pH and composition of clinically used in vitro preimplantation embryo culture media

Abstract Study question To study the pH and uterine fluid composition in the human uterus three days after a positive LH test or ovum pick-up. Summary answer The uterine pH three days after a positive LH test or ovum pick-up was 6.8. Concentrations of 37 components were determined in uterine fluid. What is known already In assisted reproductive technologies (ART), the in vitro culture of human preimplantation embryos is known to play a crucial role in the success of In Vitro Fertilization (IVF) treatments. It affects live birth rates and child outcomes such as birth weight. Numerous commercial embryo culture media are routinely used in clinical practice worldwide, but their composition is primarily based on laboratory-based clinical insights and experimental studies with animal models. Studies on the natural human preimplantation embryo environment during the physiologically relevant time of the menstrual cycle are limited. Study design, size, duration A cross sectional clinical study was conducted to study the uterine pH and the composition of human uterine fluid. The uterine pH was measured in 53 women on the third day after LH surge (N = 46) or ovum pickup (N = 7), as that time in the menstrual cycle is associated with endometrial receptivity for embryo implantation. Twenty-two samples of aspirated uterine fluid, that were collected at the same time, were analyzed for the concentrations of thirty-seven components. Participants/materials, setting, methods Healthy women of reproductive age with normal functional uterine anatomy were included. Uterine pH was determined using pH probe inserted through outer sheath of an Embryo Transfer (ET) catheter at the lithotomy position. Uterine fluid was then aspirated using a syringe connected to outer sheath of this ET catheter. Collected samples were analyzed using a Cobas 8000 chemistry analyser and ultra-performance liquid chromatography-tandem mass spectrometry. Main results and the role of chance The mean pH in the uterine cavity on the third day after a positive LH test (6.75 ± 0.22) or ovum pick-up (6.82 ± 0.18) was clearly lower than the pH used in routine clinical practice for preimplantation embryo culture (pH 7.3 ± 0.1). Calcium, phosphate, lactate(L), pyruvate, albumin and 21 amino acids were present at different concentrations in human uterine fluid compared to most commonly used preimplantation embryo culture media. Immunoglobulins, citrulline, ornithine and uric acid were present in uterine fluid, but not in clinically used culture media. Limitations, reasons for caution As the uterine cavity is only one part of the reproductive tract, our measurements do not fully represent the complete in vivo environment of preimplantation embryo development. We only studied 37 components, recognizing that uterine fluid likely contains additional components. Wider implications of the findings The observed differences between the uterine pH and the composition of uterine fluid and the pH and composition of preimplantation embryo culture media used in routine clinical practice could be a starting point to further optimize in vitro embryo culture in ART treatments. Trial registration number Not applicable

Design, Synthesis, Nematicidal Evaluation, and Molecular Docking Study of Pyrano[3,2-c]pyridones against Meloidogyne incognita.

Root-knot nematodes pose a serious threat to crops by affecting production and quality. Over a period of time, substantial work has been done toward the development of effective and environmentally benign nematicidal compounds. However, due to the inefficiencies of previously reported synthetics in achieving the target of safe, selective, and effective treatment, it is necessary to develop new efficacious and safer nematicidal agents considering human health and environment on top priority. This work aims to highlight the efficient and convenient l-proline catalyzed synthesis of pyrano[3,2-c]pyridone and their use as potential nematicidal agents. In vitro results of larval mortality and egg hatching inhibition revealed maximum nematicidal activity against Meloidogyne incognita from compounds 15b, 15m, and 15w with LC50 values of 28.8, 46.8, and 49.18 μg/mL at 48 h, respectively. Under similar conditions, pyrano[3,2-c]pyridones derivatives 15b (LC50 = 28.8 μg/mL) was found at par with LC50 (26.92 μg/mL) of commercial nematicide carbofuran. The in vitro results were further validated with in silico studies with the most active compound 15b nematicidal within the binding to the pocket of acetylcholine esterase (AChE). In docking, binding free energy values for compound 15b were found to be -6.90 kcal/mol. Results indicated that pyrano[3,2-c]pyridone derivatives have the potential to control M. incognita.

Ecological assessment and identification strategy of key conservation areas: A case study of the south Taihang Mountains region

The aim of this study was to foster the harmonious advancement of economic development and ecological preservation as well as address concerns regarding the inadequate protection of flagship species, insufficient coverage of protected areas, and anthropogenic influences in the evaluation and establishment of nature reserves. Using the southern Taihang Mountain region as a case, parameters such as habitat prediction, ecosystem services, landscape diversity, and human disturbances were comprehensively evaluated. Data from multiple sources were integrated to identify and demarcate key conservation areas as well as priority conservation areas and potential conservation zones. Our findings revealed the necessity for enhancing ecological service capacity in the southern Taihang Mountain region and highlighted an unequal distribution of animal habitats. Consequently, we propose that efforts should be concentrated on the preservation of flagship species and the creation of key conservation areas that surpass administrative borders. Based on the assessment results, it is advisable to implement conservation strategies tailored for different geomorphic environments within the key conservation areas, accompanied by relevant functional zoning. In this study, we introduce a systematic approach for assessing and designating pivotal conservation zones. Additionally, this study achieves large-scale habitat distribution predictions through minor terrestrial surveys, making it applicable to regions with pronounced anthropogenic disturbance as well as high-elevation mountainous areas. This approach enables enhanced connectivity of nature reserves and facilitates coordinated ecological conservation both within and beyond reserve boundaries, leading to an improved natural environment for humans and the creation of liberated, suitable habitats for protected animals.

Economic Determinants of Smart and Sustainable Urban Development: What Answers Does the Cities in Motion Index Give?

The goal of the article is to determine which components of sustainable and smart development of urban areas are the most important for the economy of a city. For this, regression, cluster and discriminant analysis are applied, using the data of the ranking positions of 180 cities of the world according to the Cities in Motion Index (CIMI) and its components for 2022. The Stata and Statgraphics 19 software packages are used for the calculations. The statistical significance of the input data is confirmed using descriptive statistics, and the normality of the data distribution was determined according to the Shapiro-Wilk test. A regression analysis (based on the least squares method) of the influence of the integral value of CIMI and its components (Human capital, Social cohesion, Environment, Governance, Urban planning, International profile, Technology, Mobility and Transportation) on its first component – Economy, is carried out. It testifies that only four indicators have a statistically significant impact: Cities in Motion, Environment, Urban planning, and International profile. Multiple regression, constructed using the strict screening procedure, confirms these findings; and discriminant analysis proves that the regression equation coefficients is used to predict the Economy variable. Analysis of Spearman’s and Kendall’s correlation matrices prove a close relationship between the Economy, Human capital, Governance, and Cities in motion; direct dependence between Cities in motion and such indicators as Technology, Urban planning, and International profile; average direct connection between Economy, Social cohesion and Mobility and transportation. Cluster analysis using the k-means method in the R Studio software environment made it possible to distinguish eight clusters of cities according to their ranking positions in relation to various parameters of the CIMI index (their number was calculated according to the Sturgess formula, and the optimality of their number is confirmed by the agglomeration scheme according to the Ward method). For the cities of the first cluster (17 cities, 9.44% of the total number analyzed, mostly world capitals), Cities in motion has the greatest impact on the Economy component, while Mobility and Transportation has a lesser impact; for the cities of the second cluster (23 cities, 12.78%, mostly large cities of the United States and China) it is Technology that has the greatest impact; for cities of the third cluster (35 cities, 19.44%, primarily powerful regional centers)it is Cities in motion, International profile, Mobility and transportation, Social cohesion, and Urban planning; for clusters four (9 cities, 5%) and five (6 cities, 3.33%), the regressions are not significant, so these clusters require further study for each city separately; for the cities of the sixth cluster (33 cities, 18, 33%, mostly developed European cities) the most important are Cities In motion, Environment, Governance, Mobility and transportation, Social cohesion, and Urban planning; for the cities of the seventh cluster (10 cities, 5.56%) – Human capital, Social cohesion, and Technology; for cities in the eighth cluster (47 cities, 26.11%, mostly cities facing economic obstacles to their development) – Cities in motion, Environment, Technology, and Urban planning. The discriminant analysis shows that the Environment indicator has the greatest impact on the division of clusters into groups.

ОПТИМИЗАЦИЯ СИСТЕМЫ ДОСТАВКИ ПЕРСОНАЛА НА ГОРНОРУДНЫЕ КОМПЛЕКСЫ АРКТИЧЕСКИХ ТЕРРИТОРИЙ С ПОМОЩЬЮ ИМИТАЦИОННОГО МОДЕЛИРОВАНИЯ

В исследовании анализируется возможность перевода вахтовых автобусов, используемых для доставки персонала, участвующего в разработке месторождений Арктической зоны, на газомоторное топливо с использованием имитационных моделей. The strategy of spatial development of the country for the period up to 2024, based on the infrastructure of a specific type of transport, provides for the connection of the territories of settlements with modern communications; phased reconstruction and modernization; personnel, technical and technological support for interaction and digital transformation of the Russian transport complex. The development of the Russian Arctic is a priority area, since it has a significant natural resource, socio-economic and transport potential, which must be maximized, taking into account all the features of this region. It is important to develop the northern territories in such a way that the transport infrastructure of the Arctic meets the requirements in the field of comfort and safety of the human environment. This can be achieved through the use of vehicles of increased environmental friendliness and energy efficiency. The study analyzes the possibility of converting shift buses used to deliver personnel involved in the development of fields in the Arctic zone to gas motor fuel using simulation models.

Designing and developing a programmable in vitro gastrointestinal model to assess the impact of pasteurization on the bioaccessibility of lycopene nanoparticles

To evaluate potential health impact of engineered nanoparticles, evaluation of digestion effect is necessary. Digestion requires micro-management and sequential execution of complex enzymatic mechanisms with controlled human digestion environment. The objective of this study was to develop an automated in-vitro digestive system to assess bio-accessibility of encapsulated lycopene nanoparticles in model fruit juice. Lycopene was encapsulated in polylactic acid (PLA-LNP) and polylactic co-glycolic acid (PLGA-LNP) and evaluated the bio-accessibility in a programmable in-vitro human digestive system operated with high sensitivity (25–30μs) and volume accuracy (97.37–100%). Pasteurized Model juice containing PLA-LNP, revealing higher bio-accessibility (100%) compared to PLGA-LNP (73%) counterparts. Absorption profile of both types of nanoparticles followed Korsmeyer-Peppas diffusion model at R2 value of 0.98. All types of nanoparticles with different treatments followed non-Fickian types of diffusion except PLGA-PLA with microwave pasteurization. This automated in-vitro digestive system holds significant potential for advancing drug discovery and delivery studies in the future.

Study on the multifunctional spatial-temporal evolution and coupling coordination of cultivated land: A case study of Hebei Province, China.

The rational use of cultivated land can guarantee food security and thus is highly important for ensuring social stability, economic development and national security. The current study investigated the multifunctional temporal and spatial variation characteristics of cultivated land and explored the spatial and temporal characteristics of the multifunction and coupling coordination degrees of cultivated land throughout Hebei Province. Based on the administrative division data, statistical yearbook data and land use status data of the impacted areas, a multifunctional evaluation index system of cultivated land was established. The CRITIC weight method and entropy weight method were used to determine the weight of the index, the comprehensive index model was used to determine the production, social security, ecology and landscape functions of cultivated land of Hebei Province in different periods, the coupling coordination model was used to explore the multifunctional coupling coordination degree of cultivated land in each county, and spatial autocorrelation analysis was performed to determine the correlation of the multifunctional coupling coordination degrees. From 2000 to 2020, the production, social security and landscape function of cultivated land in Hebei Province trended upward; the ecological function trended slightly downward. The multifunctional coupling coordination degree of cultivated land in Hebei Province trended significantly upward and changed from limited coordination to intermediate coordination. Furthermore, it exhibited strong agglomeration and a significant positive spatial correlation, forming a 'V'-type change rule of first decreasing and then increasing. Hebei Province exhibited remarkable spatial and temporal characteristics of the multifunction and coupling coordination degrees of cultivated land. Regions could thus customize different cultivated land functions to maximize the benefits of cultivated land use. The findings of this study may provide a scientific basis and theoretical support for sustainably using and managing cultivated land resources in areas with similar human geographical environments.

A Multidimensional Exploration of the Interplay between Human Health and Environmental Factors

This research article explores the relationship between human health and the environment, emphasizing the dire need for a holistic view to understand and addressing health challenges. Drawing upon interdisciplinary perspectives from environmental science, public health, epidemiology, and social sciences, the study investigates different environmental factors for example air and water quality, biodiversity loss, environment change, and pollution, and their impact on human health outcomes. Moreover, the paper discusses the relevance of ecosystem services in supporting human health and well-being and examines the potential of nature-based solutions for mitigating health risks associated with environmental degradation. human activities and health-related behaviors exert substantial pressure on the environment. Industrialization, urbanization, and unsustainable resource consumption degrade ecosystems, compromise biodiversity, and exacerbate climate change, thereby threatening environmental health. Deforestation, for instance, not only diminishes biodiversity but also amplifies the risk of zoonotic diseases transmission, as human encroachment brings humans closer to wildlife habitats. Promoting sustainable practices, such as renewable energy adoption and waste reduction, can mitigate environmental degradation while fostering public health. Additionally, enhancing access to healthcare services, particularly in underserved areas, and implementing policies to regulate pollution and safeguard natural resources are paramount. By elucidating the interconnectedness between health and the environment, this research contributes to a deeper understanding of the challenges facing global health and provides insights for developing effective policies and interventions to promote sustainable development and improve population health outcomes.

Gastric Cancer Assembloids Derived from Patient-Derived Xenografts: A Preclinical Model for Therapeutic Drug Screening.

The construction of reliable preclinical models is crucial for understanding the molecular mechanisms involved in gastric cancer and for advancing precision medicine. Currently, existing in vitro tumor models often do not accurately replicate the human gastric cancer environment and are unsuitable for high-throughput therapeutic drug screening. In this study, droplet microfluidic technology is employed to create novel gastric cancer assembloids by encapsulating patient-derived xenograft gastric cancer cells and patient stromal cells in Gelatin methacryloyl (GelMA)-Gelatin-Matrigel microgels. The usage of GelMA-Gelatin-Matrigel composite hydrogel effectively alleviated cell aggregation and sedimentation during the assembly process, allowing for the handling of large volumes of cell-laden hydrogel and the uniform generation of assembloids in a high-throughput manner. Notably, the patient-derived xenograft assembloids exhibited high consistency with primary tumors at both transcriptomic and histological levels, and can be efficiently scaled up for preclinical drug screening efforts. Furthermore, the drug screening results clearly demonstrated that the in vitro assembloid model closely mirrored in vivo drug responses. Thus, these findings suggest that gastric cancer assembloids, which effectively replicate the in vivo tumor microenvironment, show promise for enabling more precise high-throughput drug screening and predicting the clinical outcomes of various drugs.

Nature in law: the environment and sustainable development in Portugal’s democratic political-legal framework

This study focuses on the environmental issue in Portugal after the 25th of April, analysing the problem from three main perspectives: the contextual diachronic perspective of the critical Anthropocene construction; the relevance of the environmental issue at national and international level and environmental policies in the Portuguese legislative framework, involving questions relating to the normativity of environmental law, legislative discourse and the semantic value of the expressions “environmental quality”, “nature conservation”, “selfsustaining development”, “sustainable development”, “environmental education”, “natural environment”, and “human environment” as concepts associated with the evolution of environmental policies in post-April 25th Portugal. The question is that the Portuguese legal framework use of these concepts may contain an ambivalent valuation, oscillating between conservationist or environmentalist goals and developmentalist aims. The qualitative research methodology is mixed, using the exploratory and the comparative method according to the principles of concordance and difference in analysing legislative discourse.