Deformable silicone-molded multi-layer vocal folds replicas are commonly used in physical studies of vocal folds auto-oscillation. Nevertheless, few studies deal with the reproducibility or the mechanical properties of silicone moldings. In the current study, the effective elastic Young’s modulus of molded silicone bone-shaped specimens consisting of parallel and serial stacked layers is sought. The Young’s modulus of each layer is pertinent for one of the anatomical vocal folds layers whereas layer dimensions are designed so that the dimension ratio between adjacent layers varies in the range observed on human vocal folds. The effective Young’s modulus for one-layer, two-layer and three-layer specimens is then determined experimentally (between 4 kPa and 65 kPa) with uni-axial mechanical press tests or with precision loading tests so that both methods are cross-validated (difference less than 3.5 kPa). The overall layer dimension molding accuracy (1.5 mm) has no significant impact (less than 1.2 kPa) on the sought effective Young’s modulus and the molding procedure is considered reproducible. Furthermore, an analytical model approach for multi-layer stacked specimen is validated (overall accuracy of 2.4 kPa) against the experimental data.