Human Rib Research Articles

A Pilot Study of Microbial Succession in Human Rib Skeletal Remains during Terrestrial Decomposition.

ABSTRACTThe bones of decomposing vertebrates are colonized by a succession of diverse microbial communities. If this succession is similar across individuals, microbes may provide clues about the postmortem interval (PMI) during forensic investigations in which human skeletal remains are discovered. Here, we characterize the human bone microbial decomposer community to determine whether microbial succession is a marker for PMI. Six human donor subjects were placed outdoors to decompose on the soil surface at the Southeast Texas Applied Forensic Science facility. To also assess the effect of seasons, three decedents were placed each in the spring and summer. Once ribs were exposed through natural decomposition, a rib was collected from each body for eight time points at 3 weeks apart. We discovered a core bone decomposer microbiome dominated by taxa in the phylum Proteobacteria and evidence that these bone-invading microbes are likely sourced from the surrounding decomposition environment, including skin of the cadaver and soils. Additionally, we found significant overall differences in bone microbial community composition between seasons. Finally, we used the microbial community data to develop random forest models that predict PMI with an accuracy of approximately ±34 days over a 1- to 9-month time frame of decomposition. Typically, anthropologists provide PMI estimates based on qualitative information, giving PMI errors ranging from several months to years. Previous work has focused on only the characterization of the bone microbiome decomposer community, and this is the first known data-driven, quantitative PMI estimate of terrestrially decomposed human skeletal remains using microbial abundance information.IMPORTANCE Microbes are known to facilitate vertebrate decomposition, and they can do so in a repeatable, predictable manner. The succession of microbes in the skin and associated soil can be used to predict time since death during the first few weeks of decomposition. However, when remains are discovered after months or years, often the only evidence are skeletal remains. To determine if microbial succession in bone would be useful for estimating time since death after several months, human subjects were placed to decompose in the spring and summer seasons. Ribs were collected after 1 to 9 months of decomposition, and the bone microbial communities were characterized. Analysis revealed a core bone decomposer microbial community with some differences in microbial assembly occurring between seasons. These data provided time since death estimates of approximately ±34 days over 9 months. This may provide forensic investigators with a tool for estimating time since death of skeletal remains, for which there are few current methods.

A comparison of rib cortical bone compressive and tensile material properties: Trends with age, sex, and loading rate

The objectives of this study were to develop novel methods for quantifying human rib cortical bone material properties in compression and to compare the compressive material property data to existing tensile data for matched subjects. Cylindrical coupons were obtained from the rib cortical bone of 30 subjects (M = 19, F = 11) ranging from 18 to 95 years of age (Avg. = 48.5 ± 24.3). Two coupons were obtained from each subject. One coupon was tested in compression at 0.005 strain/s, while the other coupon was tested in compression at 0.5 strain/s. Load and displacement data were recorded so that the elastic modulus, yield stress, yield strain, ultimate stress, ultimate strain, elastic strain energy density (SED), plastic SED, and total SED could be calculated. All compressive material properties were significantly different between the two loading rates. An ANOVA revealed that sex alone had no significant effect on the compressive material properties. The interaction between sex and age was significant for some material properties, but this may have been a consequence of the lack of older females in the subject pool. None of the compressive material properties were significantly correlated with age, but were more correlated with sample density. This finding differed for the tensile material properties, which showed stronger correlations with age. When comparing between tension and compression, significant differences were observed for all material properties except for the total SED, once the effects of loading rate and age had been accounted for. This was the first study to quantify the material properties of human rib cortical bone in compression. The results of this study demonstrated that rib and thorax finite element models should consider the effects of loading rate, loading mode, and age when incorporating material properties published in the literature.

Rib Cortical Bone Fracture Risk as a Function of Age and Rib Strain: Updated Injury Prediction Using Finite Element Human Body Models.

To evaluate vehicle occupant injury risk, finite element human body models (HBMs) can be used in vehicle crash simulations. HBMs can predict tissue loading levels, and the risk for fracture can be estimated based on a tissue-based risk curve. A probabilistic framework utilizing an age-adjusted rib strain-based risk function was proposed in 2012. However, the risk function was based on tests from only twelve human subjects. Further, the age adjustment was based on previous literature postulating a 5.1% decrease in failure strain for femur bone material per decade of aging. The primary aim of this study was to develop a new strain-based rib fracture risk function using material test data spanning a wide range of ages. A second aim was to update the probabilistic framework with the new risk function and compare the probabilistic risk predictions from HBM simulations to both previous HBM probabilistic risk predictions and to approximate real-world rib fracture outcomes. Tensile test data of human rib cortical bone from 58 individuals spanning 17–99 years of ages was used. Survival analysis with accelerated failure time was used to model the failure strain and age-dependent decrease for the tissue-based risk function. Stochastic HBM simulations with varied impact conditions and restraint system settings were performed and probabilistic rib fracture risks were calculated. In the resulting fracture risk function, sex was not a significant covariate—but a stronger age-dependent decrease than previously assumed for human rib cortical bone was evident, corresponding to a 12% decrease in failure strain per decade of aging. The main effect of this difference is a lowered risk prediction for younger individuals than that predicted in previous risk functions. For the stochastic analysis, the previous risk curve overestimated the approximate real-world rib fracture risk for 30-year-old occupants; the new risk function reduces the overestimation. Moreover, the new function can be used as a direct replacement of the previous one within the 2012 probabilistic framework.

Experimental study exploring the factors that promote rib fragility in the elderly

Rib fractures represent a common injury type due to blunt chest trauma, affecting hospital stay and mortality especially in elderly patients. Factors promoting rib fragility, however, are little investigated. The purpose of this in vitro study was to explore potential determinants of human rib fragility in the elderly. 89 ribs from 13 human donors (55–99 years) were loaded in antero-posterior compression until fracture using a material testing machine, while surface strains were captured using a digital image correlation system. The effects of age, sex, bone mineral density, rib level and side, four global morphological factors (e.g. rib length), and seven rib cross-sectional morphological factors (e.g. cortical thickness, determined by μCT), on fracture load were statistically examined using Pearson correlation coefficients, Mann–Whitney U test as well as Kruskal–Wallis test with Dunn-Bonferroni post hoc correction. Fracture load showed significant dependencies (p < 0.05) from bone mineral density, age, antero-posterior rib length, cortical thickness, bone volume/tissue volume ratio, trabecular number, trabecular separation, and both cross-sectional area moments of inertia and was significantly higher at rib levels 7 and 8 compared to level 4 (p = 0.001/0.013), whereas side had no significant effect (p = 0.989). Cortical thickness exhibited the highest correlation with fracture load (r = 0.722), followed by the high correlation of fracture load with the area moment of inertia around the longitudinal rib cross-sectional axis (r = 0.687). High correlations with maximum external rib surface strain were detected for bone volume/tissue volume ratio (r = 0.631) and trabecular number (r = 0.648), which both also showed high correlations with the minimum internal rib surface strain (r = − 0.644/ − 0.559). Together with rib level, the determinants cortical thickness, area moment of inertia around the longitudinal rib cross-sectional axis, as well as bone mineral density exhibited the largest effects on human rib fragility with regard to the fracture load. Sex, rib cage side, and global morphology, in contrast, did not affect rib fragility in this study. When checking elderly patients for rib fractures due to blunt chest trauma, patients with low bone mineral density and the mid-thoracic area should be carefully examined.

Dietary shifts and diversities of individual life histories reveal cultural dynamics and interplay of millets and rice in the Chengdu Plain, China during the Late Neolithic (2500–2000 cal. BC)

We undertook a more comprehensive analyses than our previous study (Yi et al., International Journal of Osteoarchaeology, 2018, 28, 636-644) at the Gaoshan site (~2500-2000 cal. BC) to expand our understanding of the different roles of rice and millets to human subsistence strategies, diachronic shift of human diets, diversity of human life histories and cultural influence (dental ablation) to human population. Carbon and nitrogen isotopic analyses of human (ribs and long bones, n = 68) and animal (n = 15) bones as well as carbonized seeds from rice and millets (n = 8) were undertaken. Human bones were directly AMS-14 C dated. In addition, sequential sampling of 16 individuals with varied age and sex and cultural contexts for isotopic analysis was also conducted. The calibrated dating results (n = 27) indicate that there existed two periods of occupation at the site, ~2500-2200 BC and ~2200-1900 BC. Moderate high δ15 N values were found in the crops. Isotopic data from bone collagen suggest that the humans mainly relied on C3 -based animal protein and were supplemented by C4 -based foods. This isotopic pattern is also seen in pigs. Significant difference of human δ13 C values (Mann-Whitney U test, p < 0.05) between the two periods was observed. Compared to the bones, the isotopic data of human dentine serial sections show a much wider variability and higher δ13 C values. The crops (millets and rice) were probably manured. Human individuals subsisted on rice/millet agriculture and pigs in general. In combination with the radiocarbon dates, the increase of C3 (rice) consumption by the humans was found from the early (2500-2200 cal. BC) to late (2200-2000 cal. BC) periods, indicating the intensification of rice agriculture through time. However, four categories of human life histories during childhood are identified given different isotopic profiles of dentine sections, demonstrating that C3 (rice) and C4 (millets)-based foods played different roles in human lives. Even though, there were similar weaning practices among the human populations. It is surprising that human individuals with dental ablation, cultural characteristic in the Middle and Lower Yangtze River Valley, consumed more millets during childhood in the early period than those without dental ablation in the late period. Our study here provides novel insights into cultural dynamics and the interplay between rice and millets in rice-millet agricultural system during the Late Neolithic in Southwest China.

JOHNSON–COOK MODEL COMBINED WITH COWPER–SYMONDS MODEL FOR BONE CUTTING SIMULATION WITH EXPERIMENTAL VALIDATION

Constitutive models are widely used to predict the mechanical behavior of different kinds of materials. Although the Johnson–Cook model for bovine bone and Cowper–Symonds model for human thoracic rib and tibia was developed, the predictability of these models was found good only at particular strain rates. This study addresses this lack of information by investigating the Cowper–Symonds model, Johnson–Cook model, and Johnson–Cook model combined with Cowper–Symonds model at different strain rates to utilize in the bone cutting simulation. Specimens prepared using two rear femurs harvested from a 3.50-year-old bovine were investigated at different strain rates (0.00001–1/s). A comparative study made among the stresses predicted from these models showed 29.41%, 10.91%, and 11.11% mean absolute percentage errors using Cowper–Symonds model, and 2.03%, 7.19%, and 3.62% mean absolute percentage errors using Johnson–Cook model, respectively, at 0.0001, 0.001 and 1/s strain rates. However, the Johnson–Cook model combined with the Cowper–Symonds model predicted the stress with a maximum of only 2.03% mean absolute percentage error. The potential of each model to utilize in the orthogonal bone cutting was also evaluated using Ansys® and found that the combined model predicted the cutting force close to experimental cutting force with minimal error (5.20%). The outcomes of this study can be used in the surgical practice and osteotomy procedure before commencing actual surgery.

Subject-specific rib finite element models with material data derived from coupon tests under bending loading

Rib fractures are common thoracic injuries in motor vehicle crashes. Several human finite element (FE) human models have been created to numerically assess thoracic injury risks. However, the accurate prediction of rib biomechanical response has shown to be challenging due to human variation and modeling approaches. The main objective of this study was to better understand the role of modeling approaches on the biomechanical response of human ribs in anterior-posterior bending. Since the development of subject specific rib models is a time-consuming process, the second objective of this study was to develop an accurate morphing approach to quickly generate high quality subject specific rib meshes. The exterior geometries and cortical-trabecular boundaries of five human 6th-level ribs were extracted from CT-images. One rib mesh was developed in a parametric fashion and the other four ribs were developed with an in-house morphing algorithm. The morphing algorithm automatically defined landmarks on both the periosteal and endosteal boundaries of the cortical layer, which were used to morph the template nodes to target geometries. Three different cortical bone material models were defined based on the stress-strain data obtained from subject-specific tensile coupon tests for each rib. Full rib anterior-posterior bending tests were simulated based on data recorded in testing. The results showed similar trends to test data with some sensitivity relative to the material modeling approach. Additionally, the FE models were substantially more resistant to failure, highlighting the need for better techniques to model rib fracture. Overall, the results of this work can be used to improve the biofidelity of human rib finite element models.

Intra-skeletal vascular density in a bipedal hopping macropod with implications for analyses of rib histology.

Human ribs are thought to be less affected by mechanical strain at the microscopic level than limb bones, implying that rib remodelling better reflects bone physiological homeostasis. Here, we test the hypothesis that rib tissue will be well vascularized and thus enhance susceptibility to metabolic influence. An intra-skeletal comparison of bone vascular canal density was conducted using a macropod animal model adapted to bipedal habitual hopping. The right humerus, ulna, radius, femur, tibia, fibula, a mid-thoracic and upper-thoracic rib of an eastern grey kangaroo (Macropus giganteus) were sectioned at the midshaft, from which histological sections were prepared. Bone vascularity from a maximum of 12mm2 of sub-periosteal parallel-fibred and lamellar bone was recorded, resulting in a total of 2047 counted vessels. Vascular canal density data were corrected by cortical width, maximum length, and midshaft circumference robusticity indices computed for each bone. The fibula consistently had the highest vascular canal density, even when corrected for maximum length, cortical width and midshaft circumference robusticities. This was followed by the mid- and upper-thoracic ribs. Vascularity differences between bones were relatively consistent whether vascular canal density was controlled for by cortical width or midshaft circumference robusticities. Vascular canal density and robusticity indices were also positively and negatively correlated (p < 0.05). Results confirm that the ribs are well vascularized, which facilitates bone metabolic processes such as remodelling, but the fibula also appears to be a well vascularized bone. Future research investigating human bone metabolism will benefit from examining thoracic rib or fibula samples.

Study of Scalene Tubercle and Morphological Features of the First Rib with Clinical Implications

Aim and Objectives: The study aims to study the scalene tubercle and morphological features of the first rib with clinical importance. Materials and Methods: Two hundred and forty adult human dry first ribs of unknown sex were studied. The morphological features studied were scalene tubercle, vascular groove, oblique ridge, tubercle, and the head of the first rib. The obtained data were recorded and analyzed. Results: Scalene tubercle was absent in 13.75%, rudimentary in 23.33%, and hypertrophied in 1.67%. Of 240 first ribs, 1.25% was found with the attached cervical rib. The vascular groove was absent in 11.25%. Oblique ridge was absent in 5.83%. The head and tubercle were observed to be rudimentary in 16.67% and 12.5%, respectively. Conclusions: The findings of the present study will be useful for anatomists, anesthetists, forensic surgeons, and general surgeons for the identification of the first rib and management of anomalies related to the first rib.

Biomechanical Response Targets of Adult Human Ribs in Frontal Impacts.

Thorax injuries mainly due to rib fractures have been associated with high rates of morbidity and mortality in motor vehicle crashes. Thoracic biomechanics has been studied extensively, but there are no robust biomechanical response targets for ribs that consider age, sex, body size, and vulnerability factors. The objective of this study was to generate biomechanical targets for human rib response with respect to age, sex, and body size. Two-hundred sixty-one ribs from 171 individuals were dynamically loaded to failure in anterior-posterior bending. Force and displacement at the time of fracture in young adults were greater than in older adults (p < 0.0001). Sex differences were found in those over 40years old (p < 0.0001). Fracture force from 5th percentile female ribs was lower than 50th and 95th male (p < 0.005). Vulnerable ribs were successfully identified by examining the percentile of both force and displacement at the time of fracture in the proposed samples. The biomechanical targets generated in this study will have useful applications to computational thorax and rib models to aid in injury prevention measures.

Morphology of direct and indirect rib fractures.

Rib fractures are a common finding in legal medicine and information on the impact mechanism is relevant for trauma reconstruction. This study focuses on morphological characteristics of rib fractures resulting from direct or indirect force. Fresh human ribs (n = 312) were divided into two groups and broken through local force (direct) and bending (indirect) in anterolateral areas. The ribs were macerated, visually investigated and the results statistically analysed. The indirect fractures showed a significant larger lateral offset of the internal and external fracture ends while the fracture ends of the direct fractures were more often straight, in line. Also, the morphology of the inner and outer fracture edges was significantly related to fracture type. Direct fractures mostly had rough and jagged inner edges (tension side) and straight, smooth outer edges (compression side), whereas indirect fractures more often showed the characteristics vice versa. The results were more convincing in combination and in ribs from persons aged ≤ 75years at death. In summary, the direct and indirect rib fractures showed significantly different characteristics regarding orientation and offset of the fracture lines and roughness of the inner and outer fracture edges, which can be helpful to distinguish the traumatizing impact mechanisms in forensic autopsy routine.

A fast full-wave solver for calculating ultrasound propagation in the body

Therapeutic ultrasound is a promising non-invasive method for inducing various beneficial biological effects in the human body. In cancer treatment applications, high-power ultrasound is focused at a target tissue volume to ablate the malignant tumour. The success of the procedure depends on the ability to accurately focus ultrasound and destroy the target tissue volume through coagulative necrosis whilst preserving the surrounding healthy tissue. Patient-specific treatment planning strategies are therefore being developed to increase the efficacy of such therapies, while reducing any damage to healthy tissue. These strategies require to use high-performance computing methods to solve ultrasound wave propagation in the body quickly and accurately. For realistic clinical scenarios, all numerical methods which employ volumetric meshes require several hours or days to solve the full-wave propagation on a computer cluster. The boundary element method (BEM) is an efficient approach for modelling the wave field because only the boundaries of the hard and soft tissue regions require discretisation. This paper presents a multiple-domain BEM formulation with a novel preconditioner for solving the Helmholtz transmission problem (HTP). This new formulation is efficient at high-frequencies and where high-contrast materials are present. Numerical experiments are performed to solve the HTP in multiple domains comprising: (i) human ribs, an idealised abdominal fat layer and liver tissue, (ii) a human kidney with a perinephric fat layer, exposed to the acoustic field generated by a high-intensity focused ultrasound (HIFU) array transducer. The time required to solve the equations associated with these problems on a single workstation is of the order of minutes. These results demonstrate the great potential of this new BEM formulation for accurately and quickly solving ultrasound wave propagation problems in large anatomical domains which is essential for developing treatment planning strategies.

An assessment of the Arabic RH5 Neolithic fishing community’s mortuary practices by examining human rib cross-sections for bioerosion patterns

The purpose of this research is to offer a micro-anatomical skeletal means for understanding the funeral practices of the Neolithic fishing community of Ra’s al Hamra 5, which is located on the Arabian Peninsula. The archaeological site is located on the Oman gulf of the Arabian Sea coast near the suburb of Qurum; located near the Omani capital Muscat. Archaeological evidence dates the graveyard between 3800 BCE. and 3300 BCE. The burials focused on in this research come from Area 43, a part of the site that held partially burnt and commingled human remains. The study was conducted on 24 histological samples taken from ribs. The traces of bioerosion found on the non-burnt and burnt samples are analyzed. The percentage of bioerosion for each samplehas been documented (area of involvement; Ct.BAD/Ct.Ar.). Less than 5% of the rib cross-sections lack diagenetic alteration. Of the remaining samples, 52.2% show less than 49% diagenetic alteration of the cortical cross-sectional area, while 47.8% of the samples show greater than 50% or more of diagenetic change to the bone matrix. The variability in bioerosion indicates that different lengths of surface decomposition time were involved during funeral practices.This suggests that there may have been a degree of cultural variation employed as these Neolithic people buried their dead. Our results suggest that some of the remains were buried shortly after death. Other remains were exposed to open-air decomposition prior to both burning and burial.

Postmortem Analysis of Benzodiazepines in Human Bone by Gas Chromatography-Mass Spectrometry.

A procedure based on gas chromatography-mass spectrometry was developed for the analysis of benzodiazepines (nordiazepam, oxazepam, lormetazepam, lorazepam, clonazepam, bromazepam and alprazolam) in postmortem human ribs. Powdered bone samples, including marrow remains inside, with the internal standard diazepam-d5 were subjected to enzymatic hydrolysis with 100μL of β-glucoronidase and were incubated in sodium hydroxide for 1h in a 70°C oven. Samples underwent liquid phase extraction and ethyl acetate was used as eluent. Chromatography was performed on a fused silica capillary column and the selected-ion-monitoring mode was used for analytes determination. The method was validated in the range 0.1-0.5ng/mg (depending on the benzodiazepine) to 100ng/mg with average values of recovery, matrix effect and process efficiency ranged from 83.2 to 94.3%, from 97.3 to 102.1% and from 80.5 to 91.2%, respectively. The intra- and inter-day accuracy was <15%. The procedure was tested in rib specimens obtained during routine autopsies from 20 cases where these benzodiazepines were found in blood. Benzodiazepines were detected in the combined bone and marrow samples in 60% of cases. Lorazepam was detected in bone in the range of 0.3-0.7ng/mg, nordiazepam at 1.3-4.2ng/mg and oxazepam at 1.1-1.2ng/mg. To our knowledge, this protocol for the simultaneous analysis of these benzodiazepines is the first performed and validated using human ribs.

A Novel Approach to Scaling Age-, Sex-, and Body Size-Dependent Thoracic Responses using Structural Properties of Human Ribs.

Thoracic injuries are frequently observed in motor vehicle crashes, and rib fractures are the most common of those injuries. Thoracic response targets have previously been developed from data obtained from post-mortem human subject (PMHS) tests in frontal loading conditions, most commonly of mid-size males. Traditional scaling methods are employed to identify differences in thoracic response for various demographic groups, but it is often unknown if these applications are appropriate, especially considering the limited number of tested PMHS from which those scaling factors originate. Therefore, the objective of this study was to establish a new scaling approach for generating age-, sex-, and body size- dependent thoracic responses utilizing structural properties of human ribs from direct testing of various demographics. One-hundred forty-seven human ribs (140 adult; 7 pediatric) from 132 individuals (76 male; 52 female; 4 pediatric) ranging in age from 6 to 99 years were included in this study. Ribs were tested at 2 m/s to failure in a frontal impact scenario. Force and displacement for individual ribs were used to develop new scaling factors, with a traditional mid-size biomechanical target as a baseline response. This novel use of a large, varied dataset of dynamic whole rib responses offers vast possibilities to utilize existing biomechanical data in creative ways to reduce thoracic injuries in diverse vehicle occupants.

Generic finite element models of human ribs, developed and validated for stiffness and strain prediction – To be used in rib fracture risk evaluation for the human population in vehicle crashes

To enable analysis of the risk of occupants sustaining rib fractures in a crash, generic finite element models of human ribs, one through twelve, were developed. The generic ribs representing an average sized male, were created based on data from several sources and publications. The generic ribs were validated for stiffness and strain predictions in anterior-posterior bending. Essentially, both predicted rib stiffness and rib strain, measured at six locations, were within one standard deviation of the average result in the physical tests. These generic finite elements ribs are suitable for strain-based rib fracture risk predictions, when loaded in anterior-posterior bending.To ensure that human variability is accounted for in future studies, a rib parametric study was conducted. This study shows that the rib cross-sectional height, i.e., the smallest of the cross-sectional dimensions, accounted for most of the strain variance during anterior-posterior loading of the ribs. Therefore, for future rib fracture risk predictions with morphed models of the human thorax, it is important to accurately address rib cross-sectional height.

Ontogenetic changes to bone microstructure in an archaeologically derived sample of human ribs

There is considerable variation in the gross morphology and tissue properties among the bones of human infants, children, adolescents, and adults. Using 18 known-age individuals (nfemale =8, nmale =9, nunknown =1; birth to 21years old), from a well-documented cemetery collection, Spitalfields Christ Church, London, UK, this study explores growth-related changes in cortical and trabecular bone microstructure. Micro-CT scans of mid-shaft middle thoracic ribs are used for quantitative analysis. Results are then compared topreviously quantified conventional histomorphometry of the same sample. Total area (Tt.Ar), cortical area (Ct.Ar), cortical thickness (Ct.Th), and the major (Maj.Dm) and minor (Min.Dm) diameters of the rib demonstrate positive correlations with age. Pore density (Po.Dn) increases, but age-related changes to cortical porosity (Ct.Po) appear to be non-linear. Trabecular thickness (Tb.th) and trabecular separation (Tb.Sp) increase with age, whereas trabecular bone pattern factor (Tb.Pf), structural model index (SMI), and connectivity density (Conn.D) decrease with age. Sex-based differences were not identified for any of the variables included in this study. Some samples display clear evidence of diagenetic alteration without corresponding changes in radiopacity, which compromises the reliability of bone mineral density (BMD) data in the study of past populations. Cortical porosity data are not correlated with two-dimensional measures of osteon population density (OPD). This suggests that unfilled resorption spaces contribute more significantly to cortical porosity than do the Haversian canals of secondary osteons. Continued research using complementary imaging techniques and a wide array of histological variables will increase our understanding of age- and sex-specific ontogenetic patterns within and among human populations.

Human embryonic ribs all progress through common morphological forms irrespective of their position on the axis.

We aimed to analyze the morphogenesis of all ribs from 1st to 12th rib pairs plus vertebrae to compare their differences and features according to the position along the cranial-caudal axis during the human embryonic period. Rib pair formation was analyzed using high-resolution digitalized imaging data (n = 29) between Carnegie stage (CS) 18 and CS23 (corresponding to ED13-14 in mouse; HH29-35 in chick). A total of 348 rib pairs, from 1st to 12th rib pairs of each sample were subjected to Procrustes and principal component (PC) analyses. PC1 and PC2 accounted for 76.3% and 16.4% (total 92.7%) of the total variance, respectively, indicating that two components mainly accounted for the change in shape. The distribution of PC1 and PC2 values for each rib showed a "fishhook-like shape" upon fitting to a quartic equation. PC1 and PC2 value position for each rib pair moved along the fitted curve according to the development. Thus, the change in PC1 and PC2 could be expressed by a single parameter using a fitted curve as a linear scale for shape. Human embryonic ribs all progress through common morphological forms irrespective of their position on the axis.

Effects of sex, age, and two loading rates on the tensile material properties of human rib cortical bone

The objective of this study was to evaluate the effects of sex, loading rate, and age on the tensile material properties of human rib cortical bone over a wide range of subject demographics. Sixty-one (n = 61) subjects (M = 32, F = 29) ranging in age from 17 to 99 years of age (Avg. = 56.4 ± 26.2 yrs) were used in this study. Two rectangular coupons of cutaneous rib cortical bone were obtained from each subject and milled into dog-bone coupons for testing. For each subject, one coupon was tested to failure in tension on a material testing system at a targeted strain rate of 0.005 strain/s, while the other coupon was tested at 0.5 strain/s. A reaction load cell was used to measure axial load, and an extensometer was used to measure displacement within the gage length of the coupon. Data were obtained from fifty-eight (n = 58) subjects at 0.005 strain/s and fifty-eight (n = 58) subjects at 0.5 strain/s, with fifty-five (n = 55) matched pairs. The elastic modulus, yield stress, yield strain, failure stress, failure strain, ultimate stress, elastic strain energy density (SED), plastic SED, and total SED were then calculated for each test. There were no significant differences in material properties between sexes and no significant interactions between age and sex. In regard to the differences in material properties with respect to loading rate, yield stress, yield strain, failure stress, ultimate stress, elastic SED, plastic SED, and total SED were significantly lower at 0.005 strain/s compared to 0.5 strain/s. Spearman correlation analyses showed that all material properties had significant negative correlations with age at 0.005 strain/s except modulus. At 0.5 strain/s, all material properties except yield strain had significant negative correlations with age. Although the results revealed that the material properties of human rib cortical bone varied significantly with respect to chronological age, the R2 values only ranged from 0.15 to 0.62, indicating that there may be other underlying variables that better account for the variance within a given population. This is the first study to analyze the effects of sex, loading rate, and age on tensile material properties of human rib cortical bone using a reasonably large sample size. Overall, the results of this study provide data that will allow FEMs to better model and assess differences in the material response of the rib cage for nearly all vehicle occupants of driving age.

The influence of the chosen in vitro bone simulation model on intraosseous temperatures and drilling times

There is no consensus about the most suitable in vitro simulating material investigating heat generation during bone preparation. The aim was to compare heat increases and drilling times of bone removals in different bone simulating materials and to compare them to fresh human cadaver bone. A cavity was drilled in the following samples: (1) bovine rib; (2) pig rib; (3) 20 PCF (lb/ft3) polyurethane (PU) block with 3 mm (50 PCF) cortical layer; (4) 20PCF PU without cortical; (5) 30 PCF PU with 2 mm (40 PCF) cortical; (6) 30 PCF PU with 1 mm (40 PCF) cortical; (7) 30PCF PU without cortical; (8) poly-methyl-methacrylate (PMMA); (9) fresh human cadaver rib. Data were analyzed with ANOVA followed by Tukey’s post hoc tests. P < 0.05 was considered significant. Highest heat increases and slowest drilling times were found in bovine ribs (p < 0.001). Regarding temperatures, human ribs were comparable to the pig rib and to PUs having cortical layers. Considering drilling times, the human rib was only comparable to the 20 PCF PU with 3 mm cortical and to 30 PCF PU without cortical. By the tested in vitro bone removals, only the 20 PCF PU with 3 mm cortical was able to simulate human ribs, considering both temperature increases and drilling times.