Various combinations of digestive lipases were tested in vitro under conditions simulating the earlier phases of gastrointestinal lipolysis in the stomach and the duodenum. A solid/liquid test meal was mixed first with either human gastric juice or a solution containing gastric lipase, followed by either the addition of human pancreatic juice and bile or the addition of a solution containing pancreatic lipase, colipase, and bile salts. The rate of lipolysis and the composition of the lipolysis products were assessed as a function of time after lipid extraction and analysis by thin-layer chromatography coupled to flame ionization detection. The lipolytic potential of a crude rabbit gastric extract (RGE) associated with porcine pancreatic extract (PPE) was assessed and compared with the rates of lipolysis of the meal triacylglycerols by human digestive lipases recorded under the same in vitro conditions. RGE combined with PPE appeared to be a good substitute for human gastric and pancreatic lipases. RGE and PPE could therefore be used to simulate the gastrointestinal lipolysis of various foods and emulsions in vitro, as well as that of pharmaceutical lipid formulations.