Human-modified Environments Research Articles

Striped Field Mouse Invading Human-Modified Environments of Lithuania during Last Five Decades

The striped field mouse (Apodemus agrarius) is expanding in several European countries, but the details of this process are not always documented due to a lack of long-term studies. We conducted an analysis of changes in relative abundances and proportions of A. agrarius in small mammal communities in nine different habitat groups in Lithuania during 1975–2023. We found an increase in the abundance and proportion of A. agrarius, increasing synanthropization of the species, and tolerance to anthropogenization of habitats. Temporal variations in the relative abundance and proportion of species were observed, with lower values in February–July and higher values in September–January. The main finding is a remarkable increase in species presence in the country after the 1990s, with a peak observed in the 2010s–2020s, confirmed by eight local studies. During these decades, it was the third most abundant species, representing 19.8–20.0% of the individuals caught. It is also important to note that A. agrarius has recently been most abundant in commensal habitats (0.029 ± 0.006 individuals/trap day), with the highest species proportion in agricultural areas (24.8 ± 1.8%). There are many indications that the expansion of A. agrarius in Lithuania is becoming invasive.

Exploring black kite (Milvus migrans) dynamics: Seasonal abundance and habitat preferences in an urban gradient

Urban expansion globally has altered biodiversity by transforming urban habitats, leading to habitat loss and changes in ecological systems. Black kites (Milvus migrans) are adaptable raptors across diverse landscapes, from natural to highly urbanized areas. This study focuses on Gujranwala City, Pakistan, a unique habitat offering insights into black kite ecology along an urban gradient. The research assesses seasonal abundance, temperature and humidity impacts, behavioral activities, and habitat preferences of black kites. Results show higher abundance in urban areas, influenced by environmental factors and food availability from anthropogenic sources. Black kites exhibit time-dependent associations with temperature and humidity, especially before sunset. Additionally, a significant correlation is observed between black kite abundance and solid waste components, particularly food and plastic waste, in the urban area. These findings contribute to understanding urban ecology, highlighting the adaptability of black kites to human-modified environments and the potential implications for conservation and management strategies.

Navigating the Fusarium species complex: Host-range plasticity and genome variations

The Ascomycete genus Fusarium, first introduced by Link in 1809, currently consists of 431 species and 3558 unclassified isolates and hybrids (according to NCBI Taxonomy lists). Collectively, these fungi have diverse lifestyles and infection cycles exploiting a wide range of environments, hosts, ecological niches, and nutrient sources. Here, we carried out a pan-Fusarium species review to describe and explore the glamorous, and the less attractive niches, exploited by pathogenic and endophytic species. We survey species that infect plant, human, animal and/or invertebrate hosts, free-living non-pathogenic species dwelling in land, air or water-based natural ecosystems, through to those species that exploit human-modified environments or are cultivated in industrial production systems. Fully sequenced, assembled and annotated reference genomes are already available for 189 Fusarium species, many at chromosome scale. In addition, for some of the world's most important species extensive single species pangenomes or closely related formae speciales genome clusters are readily available. Previous comparative genomics studies have focussed on taxonomically restricted clusters of Fusarium species. We now investigate potential new relationships between these vastly contrasting Fusarium biologies, niches and environmental occupancies and the evolution of their respective genomes.

PREDATION OF Aspidoscelis guttatus (TEIIDAE) BY Oxybelis microphthalmus (COLUBRIDAE) IN AN URBAN ENVIRONMENT OF PUERTO ESCONDIDO, OAXACA, MEXICO

We record a new prey species for Oxybelis microphthalmus, based on an adult specimen preying on an adult male of Aspidoscelis guttatus lizard. Although three species of lizards in the genus Aspidoscelis have been reported as prey for O. microphthalmus, we did not find any information related to the predation of this lizard species. Therefore, our observation represents the first documented record of interactions between O. microphthalmus and A. guttatus in a human-modified environment.

A Comparative Study of Behaviour, Breeding and Conservation Status of Species of Sparrows Found in India: A Review

Sparrows (Passeridae) are small passerines found in human-modified environments worldwide. This review examines the behavior, breeding patterns, and conservation status of three sparrow species in India: House Sparrow (Passer domesticus), Eurasian Tree Sparrow (Passer montanus), and Chestnut-shouldered Petronia (Petronia xanthocollis). These species exhibit unique ecological niches and behaviors, from urban adaptability in House Sparrows to arboreal tendencies in Eurasian Tree Sparrows and sociable behaviors in Chestnut-shouldered Petronias. Sparrows face significant conservation challenges due to habitat loss, pollution, climate change, and urbanization. House Sparrows and Eurasian Tree Sparrows, although globally classified as "Least Concern," experience regional declines. Chestnut- shouldered Petronias are impacted by habitat fragmentation and degradation. Conservation efforts in India include nest box installations, public education, habitat restoration, and policy advocacy. The primary factors contributing to sparrow population declines are habitat degradation and reduced food availability, exacerbated by pollution and climate change. Effective conservation requires a holistic approach, integrating habitat preservation, pollution control, and community engagement. Continuous monitoring and adaptive management strategies are essential to mitigate these threats and ensure the long-term survival of sparrows, underscoring their ecological and cultural importance. Collaborative efforts among researchers, conservationists, and policymakers are crucial to safeguarding these species and their habitats.

Cyanobacteria in the Anthropocene: Synanthropism forged in an era of global change.

The Anthropocene has driven a transformative era where human activities exert unprecedented influence on Earth's biosphere. Consequently, synanthropic organisms, adept at thriving in human-modified environments, have emerged. While well studied in terrestrial ecosystems, the presence and ecological importance of synanthropic species in aquatic ecosystems, specifically among cyanobacteria, are less understood. Cyanobacteria blooms, notorious for their detrimental effects on ecosystems and human health, are increasing in frequency and intensity globally. In this perspective, we explore the evidence supporting this rise of cyanobacteria blooms, emphasizing the roles of human-induced eutrophication and climate change on select cyanobacteria genera. Cyanobacteria are not a monolith, with certain genera showing an observable increase within anthropogenically modified environments. We propose the establishment of a new sub-branch of phycology that explicitly investigates the ecology and physiology of synanthropic cyanobacteria. Understanding the intricate interactions between synanthropic species and human populations is imperative for managing human-altered ecosystems and conserving freshwater resources, particularly in the face of increasing global water insecurity. PRACTITIONER POINTS: The rise in cyanobacteria blooms is driven by a small subset of human-adapted genera-synanthropic cyanobacteria. Research is needed to characterize synanthropic cyanobacteria, which will aid in developing tailored management approaches. A paradigm shift from domesticating to "rewilding" landscapes and modifying behaviors to facilitate cohabitation are solutions to reducing risks.

Risk-sensitive learning is a winning strategy for leading an urban invasion

In the unpredictable Anthropocene, a particularly pressing open question is how certain species invade urban environments. Sex-biased dispersal and learning arguably influence movement ecology, but their joint influence remains unexplored empirically, and might vary by space and time. We assayed reinforcement learning in wild-caught, temporarily captive core-, middle-, or edge-range great-tailed grackles—a bird species undergoing urban-tracking rapid range expansion, led by dispersing males. We show, across populations, both sexes initially perform similarly when learning stimulus-reward pairings, but, when reward contingencies reverse, male—versus female—grackles finish ‘relearning’ faster, making fewer choice-option switches. How do male grackles do this? Bayesian cognitive modelling revealed male grackles’ choice behaviour is governed more strongly by the ‘weight’ of relative differences in recent foraging payoffs—i.e., they show more pronounced risk-sensitive learning. Confirming this mechanism, agent-based forward simulations of reinforcement learning—where we simulate ‘birds’ based on empirical estimates of our grackles’ reinforcement learning—replicate our sex-difference behavioural data. Finally, evolutionary modelling revealed natural selection should favour risk-sensitive learning in hypothesised urban-like environments: stable but stochastic settings. Together, these results imply risk-sensitive learning is a winning strategy for urban-invasion leaders, underscoring the potential for life history and cognition to shape invasion success in human-modified environments.

Risk-sensitive learning is a winning strategy for leading an urban invasion.

In the unpredictable Anthropocene, a particularly pressing open question is how certain species invade urban environments. Sex-biased dispersal and learning arguably influence movement ecology, but their joint influence remains unexplored empirically, and might vary by space and time. We assayed reinforcement learning in wild-caught, temporarily captive core-, middle-, or edge-range great-tailed grackles-a bird species undergoing urban-tracking rapid range expansion, led by dispersing males. We show, across populations, both sexes initially perform similarly when learning stimulus-reward pairings, but, when reward contingencies reverse, male-versus female-grackles finish 'relearning' faster, making fewer choice-option switches. How do male grackles do this? Bayesian cognitive modelling revealed male grackles' choice behaviour is governed more strongly by the 'weight' of relative differences in recent foraging payoffs-i.e., they show more pronounced risk-sensitive learning. Confirming this mechanism, agent-based forward simulations of reinforcement learning-where we simulate 'birds' based on empirical estimates of our grackles' reinforcement learning-replicate our sex-difference behavioural data. Finally, evolutionary modelling revealed natural selection should favour risk-sensitive learning in hypothesised urban-like environments: stable but stochastic settings. Together, these results imply risk-sensitive learning is a winning strategy for urban-invasion leaders, underscoring the potential for life history and cognition to shape invasion success in human-modified environments.

Impact of human created environments in the pathogenic potential and antimicrobial resistance of staphylococci from wild neotropical primates in Brazil

The non-human primate (NHP) Leontopithecus rosalia is an endangered species native of Brazil and lives in forest fragments with different levels of contact with humans (natural, private and urban). Other NHPs – Callithrix spp. - were introduced by humans and co-exist and interact with the native species in these forests.To evaluate if living in or close to human-modified environments could constitute a risk for L. rosalia, we compared the prevalence, genetic background, antibiotic susceptibility and virulence gene content of staphylococci collected from the native and the introduced species from different forest fragments.We found that presence in human-dominated environments increased the colonization rate of L. rosalia with Mammaliicoccus sciuri (former Staphylococcus sciuri) from 18 % to 85 % (p = 0.0001) and of Callithrix spp with Staphylococcus aureus from 6 % to 100 % (p = 0.0001). According to molecular typing data obtained differences probably resulted from dissemination of these bacterial species from the invader NHP species and from humans. Changes in microbiota were paralleled by an increase in the prevalence of Panton-Valentine Leukocidin gene and in resistance to beta-lactams, macrolides and/or lincosamides as exposure to human environment increased. In particular, erythromycin resistance in S. aureus from Callithrix spp. increased from 0 % to 50 % and resistance rate to at least one antibiotic in coagulase-negative staphylococci species from L. rosalia increased from 13 % to 56 % (p = 0.0003).Our results showed that contact of native animal species with human-created environments increased the content of antimicrobial resistant and pathogenic bacteria on their commensal microbiota, which ultimately can impact on their health. ImportanceEndangered animal species are vulnerable to environmental alterations and human activities have been repeatedly identified as factors driving drastic changes in the natural landscape. It is extremely important to monitor changes in the environment surrounding protected species, because this could lead to early detection of any potential threats. In this study, we found that the contact of L. rosalia - a protected non-human primate from Brazil - with human environments is related to changes in their commensal microbiota. These included an increase in the number of pathogenic and antibiotic resistant bacteria, which have a higher potential to cause infections that are more difficult to treat. We provided evidence for the harmful impact human contact has on L. rosalia. Also, our results suggest that monitoring of commensal microbiota of protected animal species might be a useful way of sensing the risks of protected species to human exposure.

Foraging and mating behaviors of Hypsignathus monstrosus at the bat-human interface in a central African rainforest.

Studying wildlife space use in human-modified environments contributes to characterize wildlife-human interactions to assess potential risks of zoonotic-pathogens transmission, and to pinpoint conservation issues. In central African rainforests with human dwelling and activities, we conducted a telemetry study on a group of males of Hypsignathus monstrosus, a lek-mating fruit bat identified as a potential maintenance host for Ebola virus. During a lekking season in 2020, we investigated the foraging-habitat selection and the individual nighttime space use during both mating and foraging activities close to villages and their surrounding agricultural landscape. At night, marked individuals strongly selected agricultural lands and more generally areas near watercourses to forage, where they spent more time compared to forest ones. Furthermore, the probability and duration of the presence of bats in the lek during nighttime decreased with the distance to their roost site but remained relatively high within a 10 km radius. Individuals adjusted foraging behaviors according to mating activity by reducing both the overall time spent in foraging areas and the number of forest areas used to forage when they spent more time in the lek. Finally, the probability of a bat revisiting a foraging area in the following 48 hours increased with the previous time spent in that foraging area. These behaviors occurring close to or in human-modified habitats can trigger direct and indirect bat-human contacts, which could thus facilitate pathogen transmission such as Ebola virus.

Preliminary checklist of butterfly diversity from the Himachal Pradesh Agricultural University, Palampur, India

Butterflies are the bio-indicator species for monitoring the health of the ecosystem. A preliminary checklist of the butterfly diversity was prepared by a long-term survey from 2019 to 2022 in varied habitats in the agriculture landscape of the Himachal Pradesh Agriculture University (HPAU), India. The study recorded 77 butterfly species belonging to six families Nymphalidae, Pieridae, Lycaenidae, Hesperiidae, Papilionidae and Riodinidae. In addition, there are some rare records of butterfly species such as Common Wall (Lasiommata schakra), Dark Blue Tiger (Tirumala septentrionis), Ringed Argus (Callerebia annada) and Pioneer (Belenois aurota). Some butterflies are habitat specific and few also show local migration from high-elevation areas of the surrounding Dhauladhar ranges. The preliminary checklist prepared from the present study was also compared with Central University of Himachal Pradesh (CUHP) located in the similar landscape of the study area. This will help to understand the long-term effect of habitat degradation from human-modified environment and agricultural activities to facilitate effective conservation strategies to protect Himalayan ecosystem.

Retaining natural vegetation to safeguard biodiversity and humanity.

Global efforts to deliver internationally agreed goals to reduce carbon emissions, halt biodiversity loss, and retain essential ecosystem services have been poorly integrated. These goals rely in part on preserving natural (e.g., native, largely unmodified) and seminatural (e.g., low intensity or sustainable human use) forests, woodlands, and grasslands. To show how to unify these goals, we empirically derived spatially explicit, quantitative, area-based targets for the retention of natural and seminatural (e.g., native) terrestrial vegetation worldwide. We used a 250-m-resolution map of natural and seminatural vegetation cover and, from this, selected areas identified under different international agreements as being important for achieving global biodiversity, carbon, soil, and water targets. At least 67 million km2 of Earth's terrestrial vegetation (∼79% of the area of vegetation remaining) required retention to contribute to biodiversity, climate, soil, and freshwater conservation objectives under 4 United Nations' resolutions. This equates to retaining natural and seminatural vegetation across at least 50% of the total terrestrial (excluding Antarctica) surface of Earth. Retention efforts could contribute to multiple goals simultaneously, especially where natural and seminatural vegetation can be managed to achieve cobenefits for biodiversity, carbon storage, and ecosystem service provision. Such management can and should co-occur and be driven by people who live in and rely on places where natural and sustainably managed vegetation remains in situ and must be complemented by restoration and appropriate management of more human-modified environments if global goals are to be realized.

Relationships between soil and badger elemental concentrations across a heterogeneously contaminated landscape

Understanding the links between environmental and wildlife elemental concentrations is key to help assess ecosystem functions and the potential effects of legacy pollutants. In this study, livers from 448 European badgers (Meles meles) collected across the English Midlands were used to investigate the relationship between elemental concentrations in topsoils and wildlife. Mean soil sample concentrations within 2 km of each badger, determined using data from the British Geological Survey's ‘Geochemical Baseline Survey of the Environment’, were compared to badger liver elemental concentrations, focusing primarily on Ag, As, Cd, Cr, Cu, K, Mn, Pb, Se, Zn. Generally, the badgers appeared to have elemental concentrations comparable with those published for other related animals, though Cu concentrations tended to be lower than expected. While there was no relationship between soil and badger liver concentrations for most biologically essential elements, biologically non-essential elements, specifically Pb, Cd, As, and Ag, were positively correlated between soil and badger livers. Lead and Cd, the elements with the strongest relationships between soils and badger livers, were primarily elevated in badgers collected in Derbyshire, a county with a millennia-long history of Pb mining and significant Pb and Cd soil pollution. Cadmium concentrations in badgers were also, on average, almost nine times higher than the local soil concentrations, likely due to Cd biomagnification in earthworms, a dietary staple of badgers. While badgers are good models for studying associations between soil and wildlife elemental concentrations, due to their diet, burrowing behaviours, and site fidelity, all flora and fauna local to human-modified environments could be exposed to and impacted by legacy pollutants.

Environmental stress - what can we learn from chlorophyll a fluorescence analysis in woody plants? A review.

Chlorophyll a fluorescence (ChF) signal analysis has become a widely used and rapid, non-invasive technique to study the photosynthetic process under stress conditions. It monitors plant responses to various environmental factors affecting plants under experimental and field conditions. Thus, it enables extensive research in ecology and benefits forestry, agriculture, horticulture, and arboriculture. Woody plants, especially trees, as organisms with a considerable life span, have a different life strategy than herbaceous plants and show more complex responses to stress. The range of changes in photosynthetic efficiency of trees depends on their age, ontogeny, species-specific characteristics, and acclimation ability. This review compiles the results of the most commonly used ChF techniques at the foliar scale. We describe the results of experimental studies to identify stress factors that affect photosynthetic efficiency and analyse the experience of assessing tree vigour in natural and human-modified environments. We discuss both the circumstances under which ChF can be successfully used to assess woody plant health and the ChF parameters that can be useful in field research. Finally, we summarise the advantages and limitations of the ChF method in research on trees, shrubs, and woody vines.

Global effects of forest modification on herpetofauna communities.

As the area covered by human-modified environments grows, it is increasingly important to understand the responses of communities to the novel habitats created, especially for sensitive and threatened taxa. We aimed to improve understanding of the major evolutionary and ecological processes that shape the assemblage of amphibian and reptile communities to forest modifications. To this end, we compiled a global data set of amphibian and reptile surveys in natural, disturbed (burned, logged), and transformed (monocultures, polyspecific plantations) forest communities to assess the richness, phylogenetic diversity, and composition of those communities, as well as the morphological disparity among taxa between natural and modified forest habitats. Forest transformations led to a diversity reduction of 15.46% relative to the statistically nonsignificant effect of disturbances. Transformations also led to a community composition that was 39.4% dissimilar to that on natural forests, compared with 16.1% difference in disturbances. Modifications did not affect the morphological disparity of communities (p=0.167 and 0.744), and we found little evidence of taxon-specific responses to anthropic impacts. Monocultures and polyspecific plantations detrimentally affected the conservation and ecological value of both amphibian and reptile communities and altered the evolutionary processes shaping these communities, whereas forests with lower impact disturbances might, to some extent, serve as reservoirs of species. Although different mechanisms might buffer the collapse of herpetological communities, preserving remaining natural forests is necessary for conserving communities in the face of future anthropic pressures.

Coupling Data- and Knowledge-Driven Methods for Landslide Susceptibility Mapping in Human-Modified Environments: A Case Study from Wanzhou County, Three Gorges Reservoir Area, China

Landslide susceptibility mapping (LSM) can provide valuable information for local governments in landslide prevention and mitigation. Despite significant improvements in the predictive performance of LSM, it remains a challenge to be carried out in areas with limited availability of data. For example, in the early stage of road construction, landslide inventory data can be particularly scarce, while there is a high need to have a susceptibility map. This study aims to set up a novel procedure for coupling the knowledge-driven and data-driven models for LSM in an area with limited landslide inventory data. In particular, we propose a two-step approach. The first step consists of applying four data-driven models (logistic regression, decision tree, support vector machines, and random forest (RF)) to derive a regional susceptibility map. In the second step, the application of a heuristic model (analytic hierarchy process, AHP) is proposed to calculate a local susceptibility map for the areas with incomplete landslide inventories. The final landslide susceptibility map is obtained by merging the most accurate regional map (RF) with the local map. We apply this novel procedure to a landslide-prone region with developed road construction (National Highway G69) in Wanzhou district, where landslide inventory is difficult to update due to timely recovery from landslide-induced road damage. Results show that the proposed methodology allows identifying new landslide-prone areas, and improving LSM predictive performance, as demonstrated by the fact that two new landslides developed along G69 were perfectly classified in the highly susceptible areas. The results show that implementing the landslide susceptibility assessment with different geographical settings and combining them into best-sensitivity partitions is more accurate than focusing on creating new models or hybrid models.

The pollen virome of wild plants and its association with variation in floral traits and land use

Pollen is a unique vehicle for viral spread. Pollen-associated viruses hitchhike on or within pollen grains and are transported to other plants by pollinators. They are deposited on flowers and have a direct pathway into the plant and next generation via seeds. To discover the diversity of pollen-associated viruses and identify contributing landscape and floral features, we perform a species-level metagenomic survey of pollen from wild, visually asymptomatic plants, located in one of four regions in the United States of America varying in land use. We identify many known and novel pollen-associated viruses, half belonging to the Bromoviridae, Partitiviridae, and Secoviridae viral families, but many families are represented. Across the regions, species harbor more viruses when surrounded by less natural and more human-modified environments than the reverse, but we note that other region-level differences may also covary with this. When examining the novel connection between virus richness and floral traits, we find that species with multiple, bilaterally symmetric flowers and smaller, spikier pollen harbored more viruses than those with opposite traits. The association of viral diversity with floral traits highlights the need to incorporate plant-pollinator interactions as a driver of pollen-associated virus transport into the study of plant-viral interactions.

Gestão e pesquisa da biodiversidade em paisagens multifuncionais

Abstract: Despite their negative environmental impacts, human-modified environments such as agricultural and urban landscapes can have a relevant role on biodiversity conservation as complements of protected areas. Such anthropized landscapes may have endangered, valuable, and nuisance species, although most of them do not fit in any of these categories. Therefore, in such environments we must deal with the same decision-making process concerning the same possible interventions proposed by Caughley (1994) to wildlife management, which are related to biological conservation, sustainable use, control/coexistence, and monitoring. Such decision-making process should be based on good science and good governance. On such context, the first step should be to implement multifunctional landscapes, which keep their primary mission of human use, but incorporate a second but fundamental mission of biological conservation. In this study we present a summary of the research carried out at the Biota Program of Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) in this field since the late 1990's and propose priorities for biodiversity research and governance in multifunctional landscapes for the near future.

Diverse moth prey identified in the diet of the critically endangered southern bent-wing bat (Miniopterus orianae bassanii) using DNA metabarcoding of scats

Context Globally, insectivorous bats are important moderators of insect populations, including agricultural pests. However, in human-modified environments, changes to insect diversity and abundance may have detrimental impacts on bat populations. The southern bent-wing bat (SBWB; Miniopterus orianae bassanii), is a critically endangered, cave-dwelling bat with a restricted distribution across south-eastern Australia, an area now dominated by agricultural land uses. Understanding SBWB diet may highlight the role of bats in influencing insect populations in surrounding agricultural land, while simultaneously providing crucial data for conservation management of this critically endangered species. Aim To investigate the SBWB’s diet using arthropod DNA metabarcoding of scats and guano collected from seven caves across the species’ range. Methods We collected scats from bat roosts and from guano piles on cave floors during late summer and early autumn of 2019. We used PCR to amplify two short, overlapping arthropod mtDNA cytochrome oxidase subunit 1 barcodes and sequenced these using the Illumina MiSeq to identify arthropod diet species. Key results Moths (order Lepidoptera) were the most prevalent insect identified in all samples and from all sites. Many of the 67 moth species identified were associated with agricultural land use (e.g. pasture webworm (Hednota pedionoma) and armyworm (Persectania dyscrita)), and several, including the bogong moth (Agrotis infusa), are migratory, suggesting the SBWB’s diet changes seasonally. Conclusion By describing the diet of the SBWB, we have fulfilled one recommendation of the national recovery plan for the species. The SBWB preys predominantly on moths, and its diet has likely been impacted by the increase in agricultural land use across its range. Further research is required to understand its foraging habitat requirements. Implications Our findings suggest the SBWB may play a role in controlling populations of moth species considered to be agricultural pests. The wide variety of moths consumed by SBWBs could afford the species some resilience to landscape changes affecting moth assemblages. The methodological framework developed here could be applied to investigate how land-use changes may contribute to bat population declines, but also how insectivorous bats may provide important ecosystem services by controlling pest insect species in modified landscapes.

Investigating the effect of habitat modification on chronic stress in deer mice: a preliminary study

Anthropogenic habitat modification can lead to chronic stress in wildlife. This can result in immunosuppression and higher disease prevalence. Chronically stressed individuals typically have elevated baseline GCs and decreased body condition. GCs are called FGMs when excreted in feces and can be used to noninvasively evaluate stress in free-ranging wildlife. In the deer mouse (Peromyscus maniculatus)–SNV system, SNV prevalence is higher in deer mice at peridomestic settings, which are human-modified habitats. This is problematic because SNV causes a fatal disease in humans, and thus the higher SNV prevalence may lead to higher risk of infection for humans. In our study, we hypothesized that SNV prevalence would be higher in deer mice at human-modified habitats due to chronic stress. To test our hypothesis, we compared two stress measures (i.e., baseline FGMs and body condition scores) in deer mice from one peridomestic and one sylvan grid over 2 months. Captured deer mice were tagged, weighed, sexed and sampled for feces and blood and were evaluated for reproductive status and body condition before release. Blood samples were analysed for SNV antibodies, and fecal samples were evaluated for FGMs. We found higher deer mouse numbers at the sylvan grid. There were no differences in baseline FGM levels between peridomestic and sylvan populations. However, peridomestic deer mice had overall lower body condition. Given the low SNV prevalence across both grids, we were unable to examine potential correlations between SNV prevalence and chronic stress. Regardless, we conclude that deer mice at human-modified habitats may not be chronically stressed, which may suggest that higher SNV prevalence at peridomestic settings may not be the result of chronic stress. Although we did find that peridomestic deer mice had lower body condition, this may not have been related to chronic stress because there were no differences in baseline FGMs. Longer studies with more site replication are needed to validate and expand on our findings. Our preliminary study adds to the existing body of knowledge that examines relationships between stress physiology and disease prevalence in human-modified environments.