Human MCF-7 Cancer Cell Lines Research Articles

Biomedical applications of terbium oxide nanoparticles by Couroupita guianensis aubl leaves extract: A greener approach

Couroupita guianensis aubl (CGA) is a plant known for its therapeutic characteristics and extensively used in biomedicine. This paper presents a method for synthesizing terbium oxide (Tb2O3) nanoparticles (NPs) utilizing the leaves extract of CGA which is sustainable and environmentally friendly. The practical synthesis of Tb2O3 NPs was validated using UV–vis analysis, revealing a prominent peak at 246 nm and the calculated band gap value of 3.02 eV. The existence of numerous functional groups and molecular bonding interactions in the Tb2O3 NPs was confirmed using FTIR spectrum analysis. In addition, the XRD examination confirmed that the nanoparticles had a crystalline structure with dimensions of 27.3 nm. The TEM imaging revealed the particle size of around 5.63 nm. The manufactured Tb2O3 NPs were analysed for anticancer capabilities against human MCF-7 cancer cell lines. Overall, our study emphasizes the ability of green-produced Tb2O3 NPs to be versatile agents with antidiabetic, antioxidant, and anticancer effects. These nanoparticles are potentially used in biomedical applications, providing long-lasting solutions to various health-related problems.

Assessment of in vitro antioxidant and cytotoxic potentials of ethanol extract of Nymphaea nauchali

Objective: The present study was designed to evaluate ethanol extract of Nymphaea nauchali for anticancer against breast cancer cells and also to determine its antioxidant properties.
 Methods: The ethanol extract of Nymphaea nauchali was prepared using sox-let apparatus. The ethanol extract was evaluated for its in-vitro anticancer potentials against human MCF-7 cancer cell lines by MTT assay and concentration of the ethanol extract required to inhibit 50% of cell growth (IC50) was recorded. To determine antioxidant properties of the extract, Superoxide scavenging, Lipid peroxidation and DPPH methods were used and IC50 values of extract was noted.
 Results: In the present study, ethanol extract of Nymphaea nauchali has shown anticancer property by exhibiting significant IC50 values against MCF 7 cells. The extract has also shown significant IC50 values in Superoxide scavenging, Lipid peroxidation and DPPH methods and antioxidant activity was comparable to standard drug ascorbic acid.
 Conclusion: The results obtained from the present research work suggested that the ethanol extract of Nymphaea nauchali possess significant in vitro cytotoxic potentials against breast cancer cells. The results also suggested that the ethanol extract have in vitro antioxidant properties.
 Keywords: Anticancer activity, Nymphaea nauchali, IC50 value, MTT assay and antioxidant activity.

Abstract 2032: Vitamin B12 augments Ca2+ dependent cytotoxicity of diethyldithiocarbamate through excessive ER-vacuolization, oxidative stress and mitochondrial depolarization in MCF-7 cells in culture

Abstract Coordinated organization of intracellular organelles into functional networks is vital for cells. Mitochondrial and endoplasmic reticulum (ER) interaction in regulation of cell Ca2+ homeostasis is crucial for cell death and survival. Function of these intracellular organelles involved in two-way regulation of the status of major cell metabolites including protein, lipid exchange and intracellular Ca2+ homeostasis. Disulfiram and its derivatives recently attracted attention as a potential anticancer treatment (Kona et al., 2011). Combined use of hydroxycobalamin (vitamin B12b) and diethyldithiocarbamate (DDTC) demonstrated synergistic effect (combination index=0.25) and enhanced the cytotoxic effect of DDTC in number of transformed cell lines in culture (Solovieva et al., 2020). In the present work using MCF-7, human breast cancer cells in culture we demonstrate that combined action of DDTC and B12b resulted in induction of UPRER manifested by dilation of ER cisterns, increased level of polyubiquitinated proteins, and increased expression of CHOP. Confocal microscopy revealed disorganization of mitochondrial network and ER and treatment of these cells with B12b/DDTC and accompanied by mitochondrial depolarization (~50% after 6 h of exposure to drugs). Treatments also induced changes in morphology of mitochondrial network, and within 6 h of exposure, mitochondria demonstrated large amplitude swelling, characteristic feature of mitochondrial permeability transition pore opening. Drug exposure induced mitochondrial oxidative stress and increase in superoxide anion level (monitored with MitoSOX) and increased content of mitochondrial Ca2+ (xRhod-1) which almost doubled following treatment with B12b/DDTC. Short-time exposure of MCF-7 cells to mixture of drugs in Ca2+-free incubation media (2.5 mM EGTA) resulted in generation of significantly smaller size of vacuoles as compared with control. Similarly, pre-loading of cells with intracellular Ca2+ chelator (BAPTA, 5 µM, 30 min, 37oC) or inhibition of IP3 receptor(s) with 2-APB, delayed the drug-induced vacuolization. Taken together, our data indicate that the mechanism of B12b/DDTC-induced cytotoxicity in human MCF-7 cancer cell lines involves mitochondrial oxidative stress, activation of MPT pore and Ca2+ overload. Support of Funding Information: Russian Science Foundation № 19-75-20145 Citation Format: Marina E. Solovieva. Vitamin B12 augments Ca2+ dependent cytotoxicity of diethyldithiocarbamate through excessive ER-vacuolization, oxidative stress and mitochondrial depolarization in MCF-7 cells in culture [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 2032.

Mechanism of EGCG promoting apoptosis of MCF-7 cell line in human breast cancer.

The aim of the present study was to investigate the effects of epigallocatechin-3-gallate (EGCG) on the apoptosis of the human MCF-7 cancer cell line and the underlying mechanism. MCF-7 cells were divided into the control group and EGCG groups. The proliferation of MCF-7 cells in the two groups was determined using MTT and apoptosis was examined using flow cytometry. Western blot analysis and qRT-PCR were used to analyze P53 and Bcl-2 expression levels. The silencing effect of specific siRNA was evaluated using RT-PCR and western blot analysis. P53 and Bcl-2 expression levels were determined using western blot analysis in the si-P53, EGCG and EGCG-combined si-P53 groups. EGCG inhibited the proliferation of MCF-7 cells in a concentration-dependent manner and IC50 was 37.681 mol/l. The apoptotic rates were 1.37 and 5.83% (t=8.9, p=0.0124) in the blank control and treatment groups after treatment with 30 µmol/l EGCG. The RT-qPCR and western blot results demonstrated that the effect of siRNA interference was evident. The expression of P53 in the EGCG-combined si-P53 group was higher than that of the si-P53 group, but lower than the EGCG group. The Bcl-2 expression level in the EGCG-combined si-P53 group was lower than that of the si-P53 group and higher than that of the EGCG group. In conclusion, EGCG suppressed the proliferation of human MCF-7 breast cancer cells and promoted apoptosis. In addition, the underlying mechanism may be related to the P53/Bcl-2 signaling pathway.

Truffle mediated (Terfezia claveryi) synthesis of silver nanoparticles and its potential cytotoxicity in human breast cancer cells (MCF-7)

Terfezia claveryi is a species that belongs to the genera of Terfeziaceae or desert truffles, which is a family of truffles. In the present study, silver nanoparticles were synthesized from aqueous extract of T. claveryi which are in the range of 25 to 60 nm. The synthesized nanoparticles were characterized by ultraviolet-visible (UV-Vis) spectroscopy, fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (FESEM) and transmission electron microscopy (TEM). The effect of the silver nanoparticles on human breast cancer cell line has been tested. Peak absorption was recorded at 440 nm in UV-Vis spectra of silver nanoparticles. The XRD data reports that the silver nanoparticles are crystalline in nature and have face centered cubic geometry. FESEM showed the size range of synthesized silver nanoparticles as 25 to 50 nm. The TEM image represents that the majority of silver nanoparticles are in spherical shape with sizes ranging between 40 and 60 nm. The aim of the present study was to report for the first time fruit mediated synthesis of silver nanoparticles using the extract of T. claveryi and showed remarkable cytotoxicity activity against human breast MCF-7 cancer cell line. Key words: Silver nanoparticles, Terfezia claveryi, fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (FESEM), transmission electron microscopy (TEM), MCF-7 cancer cell line.

Synthesis and cytotoxic activity of certain benzothiazole derivatives against human MCF-7 cancer cell line.

A new series of benzothiazole has been synthesized as cytotoxic agents. The new derivatives were tested for their cytotoxic activity toward the human breast cancer MCF-7 cell line against cisplatin as the reference drug. Many derivatives revealed good cytotoxic effect, whereas four of them, 4, 5c, 5d, and 6b, were more potent than cisplatin, with IC50 values being 8.64, 7.39, 7.56, and 5.15μm compared to 13.33μm of cisplatin. The four derivatives' cytotoxic activity was accompanied by regulating free radicals production, by increasing the activity of superoxide dismutase and depletion of intracellular reduced glutathione, catalase, and glutathione peroxidase activities, accordingly, the high production of hydrogen peroxide, nitric oxide, and other free radicals causing tumor cell death as monitored by reduction in the synthesis of protein and nucleic acids. Most of the tested compounds showed potent to moderate growth inhibitory activity; in particular, compound 6b exhibited the highest activity suggesting it is a lead compound in cytotoxic activity.

Ki-67 expression in luminal type breast cancer and its association with the clinicopathology of the cancer.

The aim of the present study was to examine Ki-67 expression in tissue of luminal type breast cancer and to investigate the association between the Ki-67 expression level and clinicopathology of breast cancer. A total of 62 patients with breast cancer were included in the study group, whereas 30 patients undergoing a health check-up who were diagnosed with breast hyperplasia were included in the control group. Levels of Ki-67 expression in patients of the two groups were assessed using fluorescent quantitative polymerase chain reaction. The association between Ki-67 and the clinicopathology of the cancer was investigated by analyzing cancer cell proliferation and migration by reducing Ki-67 expression in the human MCF-7 cancer cell line. Compared with the controls, Ki-67 expression was significantly increased in the serum and cancer tissue of breast cancer patients (P<0.05). Ki-67 mRNA expression was significantly higher in cancer tissue than that in the corresponding paracancerous tissue of breast cancer (P<0.05). In addition, a high expression of Ki-67 was positively correlated with the clinical staging of tumor, tumor size and lymphatic metastasis of breast cancer, with statistical significance (P<0.05). In MCF-7 cells with a reduced Ki-67 expression, the proliferation activity and migration of breast cancer cells were significantly reduced (P<0.05). In conclusion, Ki-67 may be involved in promoting the genesis and development of breast cancer by affecting the proliferation and migration of cancer cells.