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Abstract
Objective: The present study was designed to evaluate ethanol extract of Nymphaea nauchali for anticancer against breast cancer cells and also to determine its antioxidant properties.
 Methods: The ethanol extract of Nymphaea nauchali was prepared using sox-let apparatus. The ethanol extract was evaluated for its in-vitro anticancer potentials against human MCF-7 cancer cell lines by MTT assay and concentration of the ethanol extract required to inhibit 50% of cell growth (IC50) was recorded. To determine antioxidant properties of the extract, Superoxide scavenging, Lipid peroxidation and DPPH methods were used and IC50 values of extract was noted.
 Results: In the present study, ethanol extract of Nymphaea nauchali has shown anticancer property by exhibiting significant IC50 values against MCF 7 cells. The extract has also shown significant IC50 values in Superoxide scavenging, Lipid peroxidation and DPPH methods and antioxidant activity was comparable to standard drug ascorbic acid.
 Conclusion: The results obtained from the present research work suggested that the ethanol extract of Nymphaea nauchali possess significant in vitro cytotoxic potentials against breast cancer cells. The results also suggested that the ethanol extract have in vitro antioxidant properties.
 Keywords: Anticancer activity, Nymphaea nauchali, IC50 value, MTT assay and antioxidant activity.
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