To analyze and identify the population of the Korean War victims by studying degraded old bones based genetic analysis, we examined 108 unrelated individual femurs except two low quality bones from total investigated 110 samples. In order to obtain significantly improved genetic profiles, amplification was performed using recently developed commercial PowerPlex® Fusion 6C system and miniSTR system. We have modified PCR condition to gain enhanced DNA profiling at a success rate. When each value of two different systems came out respectively as 92.35% and 91.05% only, it created 4.40% increased value which rated at 95.45% by using these two systems simultaneously. Especially, 100% showed at D1S1656, D2S441 and D12S391, which overlapped with miniSTR system. Total of 24 autosomal STRs had gene diversity values from 0.7751 to 0.9934; SE33, Penta E, and D2S1338 which were the best in that order. The most frequent value was 0.5170 at TPOX(8) in the frequencies ranging from 6 to 37 allele. Polymorphism information content, power of discrimination and exclusion, typical paternity index and heterozygote were the highest value at SE33, but TPOX appeared the lowest in all statistics. However the value of matching probability and homozygote showed the opposite result. Since above data anticipate that these outcomes confirm the similar patterns compared to previous reported Korean population papers, it verifies the population based genetic analysis using advanced these two systems is very helpful to increase an efficiency as well as a higher accuracy on identification of victims and also to check of identical samples.
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