Pesticides are widely used to reduce crop losses due to pests. This study (an initial part of a project on risk assessment and biomonitoring) deals with pesticide use patterns in the South-West, Cameroon. This descriptive and cross-sectional study was done using questionnaires randomly administered to 137 respondents. Twenty-one crops were recorded in the area with a total of 107 pesticides (60 active ingredients) used. Three illegally used pesticides were recorded (lindane, dimethoate and malathion). Application of pesticides in combination was quite common (42.3%). Pesticide application was mainly manual using a sprayer (96.4%) with 54% of users experiencing health problems post-application. Because of the lack of funding and training, 19.7% sprayed pesticides without protection. For the Restricted Entry Interval (REI), 43.1% entered the field in less than 12h after pesticide application. The correlation between the REI and the number of symptoms was negative and non-significant (R=-0.07, p≥0.05). The main place to store pesticides was the house (57.7%) with the exception of CDC where pesticides were kept in a pesticide store. Some respondents (54.7%) said they hadn’t received any training on pesticide application while 20.4 % of respondents failed to follow recommended doses. Surface water around farms was used by 62.1% of pesticides users for domestic purposes. Some farmers (46.7%) have once heard about pesticide related accident while 14.6% suffered from pesticide intoxication, the prevalence being significantly higher in males (p<0.05). Therefore, there is a need to regulate the pesticides sector, assess ecological risk and the bioaccumulation potential of these pesticides as well as their ability to hindrance water quality and biota.