Human Fibroblast-like Cell Research Articles

Serodiagnosis of nosematosis by immunofluorescence using cell-culture-grown organisms.

This simple procedure for the detection of serum antibodies to Nosema cuniculi in rabbits and other host species incorporates indirect immunofluorescence and uses as antigen N. cuniculi isolated from urine and cultured in a human fibroblast-like cell line derived from foetal tongue. Examination of rabbit sera from 8 institutions indicated that no institution was free from Nosema infection. The prevalence of infection in 4 separate Australian rabbit colonies varied from 25 to 75%.

Effect of triphenylmethane dyes (brilliant green, crystal violet, methyl violet) on proliferation in human normal fibroblast-like and established epithelial-like cell lines

No abstract available

Replication of murine and feline RNA-containing C-type viruses in human lymphoblastoid cells.

MURINE and feline RNA-containing C-type viruses can replicate in human fibroblast-like cell monolayers with the establishment of virus shedding carrier cultures1–4. We describe here the establishment of virus shedding carrier cultures in human lymphoblastoid cell suspensions with a murine sarcoma virus (MSV) and a feline sarcoma virus (FSV).

Effect of dilantin sodium on growth of human fibroblast-like cell cultures.

SummaryAddition of sodium diphenyl-hydantoinate (Dilantin Sodium) to tissue culture of human fibroblast-like cells resulted in stimulation of their proliferation. A similar but more limited stimulation to proliferation occurred with HeLa cells but there was no detectable effect on cells isolated from rat fibrosarcoma.

Preservation of human epithelial-like and fibroblast-like cell strains at low temperatures.

SummaryStudies of cell preservation by slow freezing in 5% glycerol medium, storage at −70°C. and rapid thawing were carried out on 17 epithelial-like and 2 fibroblast-like human cell strains (Detroit). Following this procedure, each of the strains could be quickly recovered as actively proliferating cell strains with no changes in growth rate or strain characteristics.