Human Energy Expenditure Research Articles

Urinary Norepinephrine Is a Metabolic Determinant of 24-Hour Energy Expenditure and Sleeping Metabolic Rate in Adult Humans.

Interindividual variability in 24-hour energy expenditure (24EE) during energy-balance conditions is mainly determined by differences in body composition and demographic factors. Previous studies suggested that 24EE might also be influenced by sympathetic nervous system activity via catecholamine (norepinephrine, epinephrine) secretion. Therefore, we analyzed the association between catecholamines and energy expenditure in 202 individuals from a heterogeneous population of mixed ethnicities. Participants (n = 202, 33% female, 14% black, 32% white, 41% Native American, 11% Hispanic, age: 36.9 ± 10.3 y [mean ± SD], percentage body fat: 30.3 ± 9.4) resided in a whole-room calorimeter over 24 hours during carefully controlled energy-balance conditions to measure 24EE and its components: sleeping metabolic rate (SMR), awake-fed thermogenesis (AFT), and spontaneous physical activity (SPA). Urine samples were collected, and 24-h urinary epinephrine and norepinephrine excretion rates were assessed by high-performance liquid chromatography. Both catecholamines were associated with 24EE and SMR (norepinephrine: +27 and +19 kcal/d per 10 μg/24h; epinephrine: +18 and +10 kcal/d per 1 μg/24h) in separate analyses after adjustment for age, sex, ethnicity, fat mass, fat-free mass, calorimeter room, temperature, and physical activity. In a multivariate model including both norepinephrine and epinephrine, only norepinephrine was independently associated with both 24EE and SMR (both P < .008), whereas epinephrine became insignificant. Neither epinephrine nor norepinephrine were associated with adjusted AFT (both P = .37) but epinephrine was associated with adjusted SPA (+0.5% per 1 μg/24h). Our data provide compelling evidence that sympathetic nervous system activity, mediated via norepinephrine, is a determinant of human energy expenditure during nonstressed, eucaloric conditions.

Mechanization status based on machinery utilization and workers’ workload in sweet corn cultivation in Malaysia

Agricultural machinery utilization in Malaysia still very low, especially in sweet corn cultivation, compared with the other crop production systems. In this study was estimated two methods to evaluate the mechanization status of the respective field operations in sweet corn cultivation in Malaysia. The first method was used the PCL-HRL-EGL Cartesian plot based on Production capacity, Heartbeat rate, and Energy expenditures of human labor. The second method was used mechanization index based on energy expenditures of machinery and human labor. The study aim of was to assess the mechanization status in sweet corn cultivation in Malaysia. This paper described the overall mechanization status in the cultivation of sweet corn in Malaysia, and the machinery energy, worker’s energy expenditure, and heart rate for various field operations that were involved in cultivation. The field operations include tillage, planting, fertilizing, spraying, harvesting, and cutting plants. Field capacity, and machinery energy for each of the operations were calculated. The calculated mechanization index was used in the study to describe the mechanization status in sweet corn cultivation. A mean overall mechanization index of 36.49% and aggregate machinery energy of 340.67 ± 41.99 MJ/ha were registered for the crop. Highest mechanization index, and machinery energy were acquired in the tillage operation of 94.09% and 105.35 ± 9.37 MJ/ha while the lowest mechanization index, and machinery energy were in the harvesting operation with bags of 0.83%, and 0.42 ± 0.09 MJ/ha. These calculated mechanization indexes, and PCL-HRL-EGL Cartesian Plot were used for ranking the field operations based on their priority for mechanization. The outcome of this research could be contributed to lightening human energy expenditures and improving the mechanization status ultimately.

Linkage between machine power utilization and ergonomics, with reference to reducing exertions at work.

Despite mechanization development, leafy vegetable cultivation (LVC), as a labor-intensive activity in both developed and developing countries, still suffers from heavy physical activities. The present study evaluated the human physiological strains of LVC's workers to identify relationships among contributing factors affecting human physiological strains. Thirty male workers were included in this study. Working heart rate (HR) was measured using a heart rate sensor during various operations. The time taken to treat a known area was measured using a stopwatch to calculate work speed (or field capacity (FC)) for each operation. Pearson correlation coefficient and linear regression were used to investigate the relationships among HR, heart rate ratio, FC and mechanization status (MS), and human energy expenditure rate and total energy expenditure per unit area. The highest HR was at seedbed preparing (120.1 beats/min) and lowest at manual harvesting (87.8 beats/min). Manual hoe-used operations (seedbed preparing, manure application and irrigating) were demonstrated as the critical operations concerning physiological strains. The operations performed by machine power corresponded to a high FC. Variables influencing the area treating speed (i.e. MS and FC) are negatively linked to the human energy consumed per unit area and variable changed in time unit (i.e. HR) was positively linked to the human energy expenditure speed.

Impact of Sedentary Lifestyle on Teenagers 13-25 Years in Urban Households

Through this paper the author aims to provide a high-level overview of the root cause of the issue of sedentary life. With the onset of the modern age came the onset of a new category of diseases, the diseases caused due to a sedentary lifestyle. With a fall of 30% since 1960 in jobs requiring physical activity, 80% of jobs today are sedentary or require only light activity. According to WHO, 60-85% of the population does not engage in enough activity keeping this thought in mind the author through this paper aims to shows that the rate of diseases like obesity, cardiovascular diseases and diabetes type-2 has increased substantially since the 1950s, which may be attributed to the increased availability, affordability and range of different machines for different purposes which has made life easier and at the same time brought human energy expenditure to a relative stand still.

Knowledge Attitude and Practice Regarding Maternal Health Care Services

Through this paper the author aims to provide a high-level overview of the root cause of the issue of sedentary life. With the onset of the modern age came the onset of a new category of diseases, the diseases caused due to a sedentary lifestyle. With a fall of 30% since 1960 in jobs requiring physical activity, 80% of jobs today are sedentary or require only light activity. According to WHO, 60-85% of the population does not engage in enough activity keeping this thought in mind the author through this paper aims to shows that the rate of diseases like obesity, cardiovascular diseases and diabetes type-2 has increased substantially since the 1950s, which may be attributed to the increased availability, affordability and range of different machines for different purposes which has made life easier and at the same time brought human energy expenditure to a relative stand still.

The design of sports energy expenditure measuring device based on three-dimensional motion sensor

The effectiveness of the measurement method based on accelerometer for human sports energy expenditure has been confirmed by research in recent years. In this paper, combined with the overall design requirements of micropower consumption and miniaturization, this paper describes the design scheme of a portable measuring device that uses three-dimensional accelerometer to measure sports energy expenditure. The general design idea of the measuring device and the implementation method of main functional units are discussed in detail.

A novel doubly labelled 13C, 15N amino acid method for measuring energy and protein metabolism in man

ABSTRACTA novel doubly [1-13C, α-15NH2]-labelled amino acid method (DLAAM) is presented for the determination of the CO2 production (RCO2) and energy expenditure in humans. This method is based on the simultaneous administration of [1-13C]glycine and [15N]glycine followed by the measurement of excretion kinetics of breath 13CO2 and urinary 15N. The basic idea of the DLAAM is that the unknown 13C recovery RF(13C) of the 1-13C amino acid, essential for the calculation of the net CO2 production, can be approximated by the easily measureable 15N recovery RF(15N) of the α-15NH2 labelled amino acid. In four healthy adult men (76–97 kg) the DLAAM was tested parallel to the IC and in one man (74 kg) parallel to the DLWM. Using the approximation RF(13C) ≈ RF(15N) the RCO2 (in l CO2 d–1) was calculated to 387.0 ± 30.3 (DLAAM) vs. 382.8 ± 22.6. (IC). The Bland–Altman plot shows that the difference between the DLAAM and IC of individual RCO2 is within the 95 % confidence interval (mean ± 2 SD): +4.3 ± 37.5 l CO2 d–1. We conclude that the DLAAM and IC may be used interchangeably. The physical activity level (PAL) was calculated based on the DLAAM vs. DLWM to about 1.5.

EXPERIMENTING THE ENERGY ECONOMIC VARIABLES REGARDING THE LONG-HAUL CONSEQUENCES ON INDONESIA USING VECTOR ERROR CORRECTION MODEL

The Indonesian economy has recorded solid growth in the course of recent decades, and as of late, the firm pace of economic expansion has been joined by decreased yield instability and moderately stable inflation. As a sort of rare characteristic capital, energy makes increasingly more clear imperative consequences for economic growth (EG). Furthermore, energy consumption (EC) is the real wellspring of greenhouse gas emissions. This achieves the issues of the connections among energy economic variables, which is deserving of long-haul consideration between human capital (HC), EC, CO2 emissions, and EG in Indonesia. As a close neighbour, Australia has long had critical exchange ties with Indonesia. The effect of various types of EC and HC on per capita GDP growth. The information utilized world development indicator has acquired from the World Bank database amid 1985- 2017. The examination technique utilized vector error correction model. In this mainly three tests are conducted in order to know the relationship among variables which is unit root test, co-integration and temporal Granger causality the outcomes show that apportioning consumption energy and controlling carbon emissions, are probably going to have no antagonistic impact on the genuine GDP per capita. In the meantime, empirically, the development of HC has the effect on controlling CO2 emissions and EC in Indonesia.

Resting Energy Expenditure, Insulin Resistance and UCP1 Expression in Human Subcutaneous and Visceral Adipose Tissue of Patients With Obesity

Determinants of resting energy expenditure (REE) in humans are still under investigation, especially the association with insulin resistance. Brown adipose tissue (AT) regulates energy expenditure through the activity of the uncoupling protein 1 (UCP1). White AT browning is the process by which some adipocytes within AT depots acquire properties of brown adipocytes (“brite” adipocytes) and it correlates with metabolic improvement. We analyzed determinants of REE in patients with obesity and assessed UCP1 expression as a “brite” marker in abdominal subcutaneous AT (SAT) and visceral omental AT (VAT). Clinical data, REE, free fat mass (FFM), and fat mass (FM) were determined in 209 patients with obesity. UCP1, PPARG coactivator 1 alpha (PPARGC1A), transcription factor A, mitochondrial (TFAM), T-box transcription factor 1 (TBX1), and solute carrier family 27 member 1 (SLC27A1) expression was assayed in SAT and VAT samples, obtained during sleeve gastrectomy from 62 patients with obesity. REE and body composition data were also available for a subgroup of 35 of whom. In 209 patients with obesity a multiple regression model was computed with REE as the dependent variable and sex, waist, FFM, FM, homeostasis model assessment-insulin resistance (HOMA), interleukin-6 and High Density Lipoprotein-cholesterol as the independent variables. Only FFM, FM and HOMA were independently correlated with REE (r = 0.787, AdjRsqr = 0.602). In each patient VAT displayed a higher UCP1, PPARGC1A, TFAM, TBX1, and SLC27A1 expression than SAT and UCP1 expression in VAT (UCP1-VAT) correlated with Body Mass Index (BMI) (r = 0.287, p < 0.05). Introducing UCP1-VAT in the multivariate model, we showed that FFM, HOMA, interleukin-6, High Density Lipoprotein-cholesterol, and UCP1-VAT were independent factors correlated with REE (r = 0.736, AdjRsqr = 0.612). We confirmed that REE correlates with FFM, FM and HOMA in a large cohort of patients. Our results clearly showed that UCP1-VAT expression was significantly increased in severe human obesity (BMI > 50 kg/m2) and that it behaved as an independent predictor of REE. Lastly, we suggest that an increased REE and browning in metabolically complicated severe obesity could represent an effort to counteract further weight gain.

Early Time-Restricted Feeding Reduces Appetite and Increases Fat Oxidation But Does Not Affect Energy Expenditure in Humans.

ObjectiveEating earlier in the daytime to align with circadian rhythms in metabolism enhances weight loss. However, it is unknown whether these benefits are mediated through increased energy expenditure or decreased food intake. We therefore performed the first randomized trial to determine how meal timing affects 24-hour energy metabolism when food intake and meal frequency are matched.MethodsEleven overweight adults practiced both early time-restricted feeding (eTRF; eating from 8 am-2 pm) and a control schedule (eating from 8 am-8 pm) each for four days. On the fourth day, 24-hour energy expenditure and substrate oxidation were measured by whole-room calorimetry, in conjunction with appetite and metabolic hormones.ResultseTRF did not affect 24-hour energy expenditure (Δ=10±16 kcal/day; p=0.55). Despite the longer daily fast (intermittent fasting), eTRF decreased mean ghrelin levels by 32±10 pg/ml (p=0.006), made hunger more even-keeled (p=0.006), and tended to increase fullness (p=0.06-0.10) and decrease the desire to eat (p=0.08). eTRF also increased metabolic flexibility (p=0.0006) and decreased the 24-hour non-protein respiratory quotient (Δ=−0.021±0.010; p=0.05).ConclusionsMeal timing interventions facilitate weight loss primarily by decreasing appetite rather than by increasing energy expenditure. eTRF may also increase fat loss by increasing fat oxidation.

Obesity Tissue: Composition, Energy Expenditure, and Energy Content in Adult Humans.

Chronic positive energy balance leads to obesity, and the "excess" weight is usually described as consisting solely of adipose tissue (AT) or its two components, fat and fat-free mass (nonfat cell mass, extracellular fluid). This study aimed to clarify the nature of "obesity" tissue. A total of 333 adults had AT, skin, skeletal muscle, bone, heart, liver, kidney, spleen, brain, and residual mass measured or derived using magnetic resonance imaging and dual-energy x-ray absorptiometry. First, associations between these components and AT were examined by developing multiple regression models. Next, obesity-tissue composition was developed by deriving mean component mass differences between participant groupswith normal weight (BMI < 25 kg/m2 ) and those with obesity (BMI > 29.9 kg/m2 ); respective resting energy expenditures and metabolizable energy and protein contents were calculated. AT significantly predicted organ-tissue mass in 17 of 18 multiple regression models. In addition to AT and skeletal muscle, the following associations were found: skin, liver, and bone were main contributors to obesity-tissue composition; liver, kidneys, and heart to resting energy expenditure; and skin, liver, and bone to metabolizable energy and protein contents. A pronounced sexual dimorphism was present in all three models. Obesity is characterized not only by excess AT but by increases in the masses of other "companion" organs and tissues and their related metabolic properties.

Energy Expenditure Estimation During Crutch-Orthosis-Assisted Gait of a Spinal-Cord-Injured Subject.

Determination of muscle energy expenditure by computer modeling and analysis is of great interest to estimate the whole body energy consumption, while avoiding the complex character of in vivo experimental measurements for some subjects or activities. In previous papers, the authors presented optimization methods for estimating muscle forces in spinal-cord-injured (SCI) subjects performing crutch-assisted gait. Starting from those results, this work addresses the estimation of the whole body energy consumption of a SCI subject during crutch-assisted gait using the models of human muscle energy expenditure proposed by Umberger and Bhargava. First, the two methods were applied to the gait of a healthy subject, and experimentally validated by means of a portable gas analyzer in several 5-min tests. Then, both methods were used for a SCI subject during crutch-assisted gait wearing either a passive or an active knee-ankle foot orthosis (KAFO), in order to compare the energetic efficiency of both gait-assistive devices. Improved gait pattern and reduced energy consumption were the results of using the actuated gait device. Computer modeling and analysis can provide valuable indicators, as energy consumption, to assess the impact of assistive devices in patients without the need for long and uncomfortable experimental tests.

Effect of the fatty acid composition of meals on postprandial energy expenditure: a systematic review.

The energy imbalance produced by an increase in caloric intake and/or decrease in energy expenditure induces obesity. However, the fatty acid composition of a diet can affect the metabolism in different ways, having a role in the development of obesity. To determine the effect of different fatty acids types and composition on Diet-Induced Thermogenesis (DIT) and postprandial energy expenditure in humans. A search in the PubMed and Web of Science databases, yielded a total of 269 potential articles as a first result; 254 were excluded according to the criteria. Fifteen articles were used for this systematic review. The studies analyzed report different effects of the fatty acids of the treatment on the diet-induced thermogenesis. Evidence indicates that the consumption of polyunsaturated fatty acids causes a greater DIT than saturated fatty acids. Also, the consumption of medium-chain fatty acids compared to long-chain fatty acids has been shown to increase DIT. Likewise, the use of certain oils has shown positive effects on postprandial energy expenditure, as is the case of olive oil, compared to rapeseed oil. The use of specific types of fatty acids in the everyday diet can increase postprandial energy expenditure in humans. Nevertheless, longer-term studies are required.

Human energy and time spent by women using cooking energy systems: A case study of Nepal

In most developing countries, many rural households use fuelwood and a traditional cookstove (TCS). Women are the backbone of the cooking system, as they mostly manage it. Despite several existing efficient cooking energy systems, households generally do not prefer them. Thus, our aim is to find why this is the case. We estimate the time required and human energy expenditure (HEE) for production of cooking fuel for four alternative cooking energy systems in Nepal, as a case study. The time required to produce cooking fuel for the baseline scenario (i.e. fuelwood and TCS) is 40 h/cap/yr and HEE is 41 MJ/cap/yr. System 2 (charcoal and TCS) has the highest demand for time and HEE. The results suggest that the most efficient system is System 1 (i.e. fuelwood and an improved cookstove (ICS)). However, a woman produces cooking fuel for the whole household, which multiples her time and HEE demand to the household size. This system analysis indicates a significant influence in the selection of cooking fuel due to the HEE and time demand. It concludes that in the future, more importance should be attached to the labour required from women in the cooking energy systems in the development of technological improvements.

Extreme events reveal an alimentary limit on sustained maximal human energy expenditure.

The limits on maximum sustained energy expenditure are unclear but are of interest because they constrain reproduction, thermoregulation, and physical activity. Here, we show that sustained expenditure in humans, measured as maximum sustained metabolic scope (SusMS), is a function of event duration. We compiled measurements of total energy expenditure (TEE) and basal metabolic rate (BMR) from human endurance events and added new data from adults running ~250 km/week for 20 weeks in a transcontinental race. For events lasting 0.5 to 250+ days, SusMS decreases curvilinearly with event duration, plateauing below 3× BMR. This relationship differs from that of shorter events (e.g., marathons). Incorporating data from overfeeding studies, we find evidence for an alimentary energy supply limit in humans of ~2.5× BMR; greater expenditure requires drawing down the body's energy stores. Transcontinental race data suggest that humans can partially reduce TEE during long events to extend endurance.

Workers’ rest allowance and smoothing of the workload in assembly lines

Ergonomic aspects have a crucial role in manual assembly systems. They impact on the workers’ health, final product quality and productivity. For these reasons, there is the necessity to integrate them into the assembly line balancing phase as, whereas, only time and cost variables are considered. In this study, human energy expenditures are considered as ergonomic aspects and we integrate them, for the first time, into the assembly line balancing problem type 2 through the rest allowance evaluation. We consider as an objective function the minimization of the smoothness index. Firstly, a new optimal method based on mixed integer linear programming and a new linearization methodology are proposed. Then, a heuristic approach is introduced. To complete the study, a computational experimentation is presented to validate the mathematical model and to compare the methodologies proposed in terms of computational time, complexity and solution. Additionally, we provide a detailed analysis of the impact that rest allowance evaluation can have on productivity comparing the results obtained, taking into account the rest allowance integration before, during and after the assembly balancing process.

Methodologic considerations for measuring energy expenditure differences between diets varying in carbohydrate using the doubly labeled water method

Low-carbohydrate diets have been reported to significantly increase human energy expenditure when measured using doubly labeled water (DLW) but not by respiratory chambers. Although DLW may reveal true physiological differences undetected by respiratory chambers, an alternative possibility is that the expenditure differences resulted from failure to correctly estimate the respiratory quotient (RQ) used in the DLW calculations. To examine energy expenditure differences between isocaloric diets varying widely in carbohydrate and to quantitatively compare DLW data with respiratory chamber and body composition measurements within an energy balance framework. DLW measurements were obtained during the final 2 wk of month-long baseline (BD; 50% carbohydrate, 35% fat, 15% protein) and isocaloric ketogenic diets (KD; 5% carbohydrate, 80% fat, 15% protein) in 17 men with a BMI of 25-35 kg/m2. Subjects resided 2 d/wk in respiratory chambers to measure energy expenditure (EEchamber). DLW expenditure was calculated using chamber-determined RQ either unadjusted (EEDLW) or adjusted (EEDLWΔRQ) for net energy imbalance using diet-specific coefficients. Accelerometers measured physical activity. Body composition changes were measured by dual-energy X-ray absorptiometry (DXA) which were combined with energy intake measurements to calculate energy expenditure by balance (EEbal). After transitioning from BD to KD, neither EEchamber nor EEbal were significantly changed (∆EEchamber=24±30 kcal/d; P=0.43 and ∆EEbal=-141±118 kcal/d; P=0.25). Similarly, physical activity (-5.1±4.8%; P=0.3) and exercise efficiency (-1.6±2.4%; P=0.52) were not significantly changed. However, EEDLW was 209±83 kcal/d higher during the KD (P=0.023) but was not significantly increased when adjusted for energy balance (EEDLWΔRQ=139±89 kcal/d; P=0.14). After removing 2 outliers whose EEDLW were incompatible with other data, EEDLW was marginally increased during the KD by 126±62 kcal/d (P=0.063) and EEDLW∆RQ was only 46±65 kcal/d higher (P=0.49). DLW calculations failing to account for diet-specific energy imbalance effects on RQ erroneously suggest that low-carbohydrate diets substantially increase energy expenditure. This trial was registered at clinicaltrials.gov as NCT01967563.

OR01-5 Leptin Acts through Altering Energy Intake and Lipolysis to Reduce Body Weight but Has No Effect on Energy Expenditure, Physical Activity, and Sympathetic Nervous System in Humans

It remains unknown whether leptin regulates body weight in humans and whether this occurs through actions centrally and/or peripherally i.e. by altering hormone and sympathetic nervous system (SNS) activity in humans as it has been suggested by experiments in rodents. We explored body weight and body composition changes in response to short and long term leptin administration to lean humans and explored potential underlying mechanisms by studying changes in energy intake, energy expenditure, lipolysis and hormonal and SNS changes in response to leptin administered in physiological and supraphysiological doses in lean subjects. We utilized the following study designs: a) open-label long-term leptin replacement study aiming at keeping circulating leptin levels within the physiological range and b) randomized, placebo-controlled, long-term leptin replacement study aiming at keeping total body weight stable by adjusting leptin doses in females with acquired hypoleptinemia. To further explore underlying mechanisms we also performed a) short-term placebo vs. leptin physiological replacement study during a three day long fasting-induced hypoleptinemia in lean subjects; and b) short-term leptin dose escalation study to supraphysiological levels in the fed i.e. normoleptinemic vs. in fasting-induced hypoleptinemic state for three days in both lean and overweight individuals. Long-term leptin replacement decreased body weight (p-value<0.001) through decreasing fat mass (p-value<0.001) and without affecting lean mass. Underlying mechanisms included decreased energy intake with leptin (caloric intake - leptin association r=-0.644, p-value<0.001), but not energy expenditure. Leptin also increased lipolysis demonstrated by elevated free fatty acids which were increased in response to leptin at time points that corresponded to fat mass loss (p-value=0.01). Long- but not short-term leptin replacement downregulated the renin-angiotensin-aldosterone axis at time points corresponding to maximum lipolytic effects (p-value=0.024 renin; p-value=0.001 aldosterone). Importantly, no changes in heart rate, blood pressure and body temperature were demonstrated with leptin. Thus, leptin administration decreases body weight in lean subjects, without changes to lean mass, by acting through decreasing body fat primarily through lipolysis and decreased caloric intake.

Acute behavioural and physiological responses of sheep to S/C ivermectin injection

Acute behavioural and physiological responses of sheep to S/C ivermectin injection

Effects of combined GIP and GLP-1 infusion on energy intake, appetite and energy expenditure in overweight/obese individuals: a randomised, crossover study.

Glucagon-like peptide 1 (GLP-1) reduces appetite and energy intake in humans, whereas the other incretin hormone, glucose-dependent insulinotropic polypeptide (GIP), seems to have no effect on eating behaviour. Interestingly, studies in rodents have shown that concomitant activation of GIP and GLP-1 receptors may potentiate the satiety-promoting effect of GLP-1, and a novel dual GLP-1/GIP receptor agonist was recently shown to trigger greater weight losses compared with a GLP-1 receptor agonist in individuals with type 2 diabetes. The aim of this study was to delineate the effects of combined GIP and GLP-1 receptor activation on energy intake, appetite and resting energy expenditure in humans. We examined 17 overweight/obese men in a crossover design with 5 study days. On day 1, a 50g OGTT was performed; on the following 4 study days, the men received an isoglycaemic i.v. glucose infusion (IIGI) plus saline (154mmol/l NaCl; placebo), GIP (4pmolkg-1min-1), GLP-1 (1pmolkg-1min-1) or GIP+GLP-1 (4 and 1pmolkg-1min-1, respectively). All IIGIs were performed in a randomised order blinded for the participant and the investigators. The primary endpoint was energy intake as measured by an ad libitum meal after 240min. Secondary endpoints included appetite ratings and resting energy expenditure, as well as insulin, C-peptide and glucagon responses. Energy intake was significantly reduced during IIGI+GLP-1 compared with IIGI+saline infusion (2715 ± 409 vs 4483 ± 568kJ [mean ± SEM, n = 17], p = 0.014), whereas there were no significant differences in energy intake during IIGI+GIP (4062 ± 520kJ) or IIGI+GIP+GLP-1 (3875 ± 451kJ) infusion compared with IIGI+saline (p = 0.590 and p = 0.364, respectively). Energy intake was higher during IIGI+GIP+GLP-1 compared with IIGI+GLP-1 infusion (p = 0.039). While GLP-1 infusion lowered energy intake in overweight/obese men, simultaneous GIP infusion did not potentiate this GLP-1-mediated effect. ClinicalTrials.gov NCT02598791 FUNDING: This study was supported by grants from the Innovation Fund Denmark and the Vissing Foundation.