Model Of Human Development Research Articles

Modeling of Skeletal Development and Diseases Using Human Pluripotent Stem Cells.

Human skeletal elements are formed from distinct origins at distinct positions of the embryo. For example, the neural crest produces the facial bones, the paraxial mesoderm produces the axial skeleton, and the lateral plate mesoderm produces the appendicular skeleton. During skeletal development, different combinations of signaling pathways are coordinated from distinct origins during the sequential developmental stages. Models for human skeletal development have been established using human pluripotent stem cells (hPSCs) and by exploiting our understanding of skeletal development. Stepwise protocols for generating skeletal cells from different origins have been designed to mimic developmental trails. Recently, organoid methods have allowed the multicellular organization of skeletal cell types to recapitulate complicated skeletal development and metabolism. Similarly, several genetic diseases of the skeleton have been modeled using patient-derived induced pluripotent stem cells and genome-editing technologies. Model-based drug screening is a powerful tool for identifying drug candidates. This review briefly summarizes our current understanding of the embryonic development of skeletal tissues and introduces the current state-of-the-art hPSC methods for recapitulating skeletal development, metabolism, and diseases. We also discuss the current limitations and future perspectives for applications of the hPSC-based modeling system in precision medicine in this research field.

Human Capital Management Development and Environment Policy Model on The Performance of State Civil Apparatus in Riau Province Government

This study aims to develop a conceptual framework for Competence-based Human Capital Management (HCM) and Religiosity to the Performance of the State Civil Apparatus (ASN) in the Riau Provincial Government. This study is to find the most appropriate way to develop ASN competence and fill in the limitations of previous research and gaps in competency research on ASN performance. This study uses a quantitative approach by distributing questionnaires to officials related to the development of ASN competencies, both Structural Officials ranging from Middle High Officials, Primary High Officials, Administrator, and Supervisory Officers, as well as Functional Officers related to competency development, as many as 114 respondents with using a purposive sampling approach, data collection techniques using a questionnaire with a scale of 1-5, which then uses Structural Equation Modeling-Partial Least Square (PLS) analysis with the outer Model, and inner model and processed using the SmartPLS 3. Capital Management and Supported by Religiosity. This study examines the development of competence based on Human Capital Management and Religiosity as a moderator of ASN performance. This research has never been conducted simultaneously.

Synthetic Human Embryos, Embryo Models and Embryo-like Structures in Islam

ABSTRACT A major breakthrough in developmental biology is the ex vivo generation of synthetic human embryos from stem cells. A comprehensive, in-depth bioethical analysis from a Sunni Islamic perspective reveals that the reproductive applications of synthetic human embryos contravene Islamic precepts of preserving lineage integrity (Hifz al-Nasl) due to disruption and confusion of kinship and familial relationships, similar to human cloning with somatic cell nuclear transfer. However, their non-reproductive applications in generating replacement tissues/organs, serving as in vitro experimental models of human development and disease, and testing platforms for evaluating pharmaceuticals and biomedical devices appear to align with Islamic principles.

The 3H and Spiral Dynamics Models: A Reconciliation

Abstract: This study explores the relationship between the Spiral Dynamics and the 3H (head, heart, hands) models of human growth and development, using constructs such as empathy, moral reasoning, forgiveness, and community mindedness that have been shown to have implications for education. The specific research question is, "Can a combination of multivariate statistical techniques be utilized to find an alignment between the dimensions of these models?" We focus on practical and data-driven approaches with the primary methods including factor analysis, cluster analysis, and logistic regression. Our main finding is that the proposed methodology is robust and applicable in a variety of operational scenarios. We conclude it is feasible to empirically align and reconcile dimensions of seemingly disparate theories of educational development and human evolution with a data analysis framework based on mainstream quantitative techniques that can be easily implemented using readily available statistical software packages.

Teaching as Family Life Educators in Collegiate Settings: The Application of Family Science Theories from Systemic and Chronological Perspectives

Family Science is an exciting field which offers a multitude of career pathways, including Family Life Educator (FLE) and the Certified Family Life Educator (CFLE) credential. In this article, I will discuss how, as a Family Life Educator in a university setting, I employ Bronfenbrenner's bioecological model of human development (Bronfenbrenner & Morris, 2006), Elder's (1998) life course perspective, and the concept of intersectionality in teaching family science courses. The article will conclude with discussions of the strengths, limitations, and lessons learned.

A Facile, Transfection-Free Approach to siRNA Delivery in In Vitro 3D Spheroid Models.

Cell culture has long been essential for preclinical modeling of human development and disease. However, conventional two-dimensional (2D) cell culture fails to faithfully model the complexity found in vivo, and novel drug candidates that show promising results in 2D models often do not translate to the clinic. More recently, three-dimensional (3D) cell culture models have gained popularity owing to their greater physiological relevance to in vivo biology. In particular, 3D spheroid models are becoming widely used due to their ability to mimic solid tumors, both in architecture and gradation of nutrients distributed from the outer, proliferative layers into the inner, quiescent layers of cells. Similar to in vivo tumors, cell lines grown in 3D spheroid models tend to be more resistant to antitumor drug treatments than their 2D cultured counterparts, though distinct signaling pathways and gene targets conferring this resistance have yet to be fully explored. RNA interference (RNAi) is an effective tool to elucidate gene function and discover novel druggable targets in 2D models; however, only a few studies have successfully performed RNAi in complex 3D models to date. Here, we demonstrate efficient RNAi-mediated knockdown using "transfection-free" Dharmacon Accell siRNAs in three spheroid culture models, in the presence or absence of the extracellular matrix. This methodology has the potential to be scaled up for complex arrayed screening experiments, which may aid in the identification of novel druggable targets with greater clinical relevance than those identified in 2D experiments. © 2024 Dharmacon, Inc. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Generation of 3D spheroids in matrix-free ULA plates Alternate Protocol 1: Generation of Matrigel matrix-embedded 3D spheroids Alternate Protocol 2: Generation of GrowDex hydrogel-embedded 3D spheroids Basic Protocol 2: Delivery of siRNA and collection of matrix-free 3D spheroids Alternate Protocol 3: Delivery of siRNA and collection of matrix-embedded spheroids Basic Protocol 3: RNA and protein extraction from spheroids for characterization of gene knockdown.

MILESTONES IN THE DEVELOPMENT OF ORGANIZATIONAL MODELS BASED ON THE THEORY OF SPIRAL DYNAMICS

The article examines the milestones of the development of the business environment through the prism of industrial revolutions and the theory of spiral dynamics of organizational development. The past influences the present. The present shapes the future. The world is a complex, interconnected whole that is difficult to represent and understand, especially regarding complex socio-economic processes and organizational systems, including enterprises. The first radical change began precisely with the agrarian revolution when people domesticated animals as a basis for the transition from gathering to agriculture. After the agrarian revolution, radical industrial changes began in the 18th century. The first industrial revolution (from the 1760s to the 1840s) - the invention of the steam engine and the construction of railways. The second industrial revolution (the end of the 19th and the beginning of the 20th century) - the emergence of mass production thanks to the invention of electricity and the introduction of the conveyor. The third industrial revolution, from the 1960s, - was the emergence of semiconductors and their use in large electronic computing machines, personal computers, and the Internet. The fourth industrial revolution began at the beginning of the 21st century and is characterized by the emergence of "smart enterprises". The content and essential components of these periods imposed and imposed their understanding on the development of organizational models and the way of interaction between stakeholders and the economic entities themselves, which arose at the later stages of development and are developing under the influence of global business trends. Deepening the understanding of the nature of modern approaches in management aimed at the development of organizational systems allows turning to the past and investigating issues of organizational models on the way of the history of human development based on the theory of spiral dynamics (Don et al.), which is based on a dynamic model of human development and evolution of her consciousness, change of fundamental values. So, from the standpoint of the concept of systemic perfection of a business organization, based on the theory of spiral dynamics, its consideration can be started in the system-wide understanding of the system state, which corresponds to a greater extent to the content of perfection - from the orange stage and more/less corresponds to the current trends of the fourth industrial revolution. A significant strategic gap occurs based on the mismatch between the stage of development according to the milestones of industrial revolutions and the organization's current state in relation to the theory of spiral dynamics. For example, a red organization that functions in the fourth industrial revolution era. Management of changes in paradigms of thinking is the art of management.

Retinoic acid induces human gastruloids with posterior embryo-like structures

Gastruloids are a powerful in vitro model of early human development. However, although elongated and composed of all three germ layers, human gastruloids do not morphologically resemble post-implantation human embryos. Here we show that an early pulse of retinoic acid (RA), together with later Matrigel, robustly induces human gastruloids with posterior embryo-like morphological structures, including a neural tube flanked by segmented somites and diverse cell types, including neural crest, neural progenitors, renal progenitors and myocytes. Through in silico staging based on single-cell RNA sequencing, we find that human RA-gastruloids progress further than other human or mouse embryo models, aligning to E9.5 mouse and CS11 cynomolgus monkey embryos. We leverage chemical and genetic perturbations of RA-gastruloids to confirm that WNT and BMP signalling regulate somite formation and neural tube length in the human context, while transcription factors TBX6 and PAX3 underpin presomitic mesoderm and neural crest, respectively. Looking forward, RA-gastruloids are a robust, scalable model for decoding early human embryogenesis.

A brief chronicle of research on human pluripotent stem cells.

Today, human pluripotent stem cell technologies find widespread application across biomedical research, as models for early human development, as platforms for functional human genomics, as tools for the study of disease, drug screening and toxicology, and as a renewable source of cellular therapeutics for a range of intractable diseases. The foundations of this human pluripotent stem cell revolution rest on advances in a wide range of disciplines, including cancer biology, assisted reproduction, cell culture and organoid technology, somatic cell nuclear transfer, primate embryology, single-cell biology, and gene editing. This review surveys the slow emergence of the study of human pluripotency and the exponential growth of the field during the past several decades.

Risk patterns of bullying perpetration and victimization among children

ObjectivesThis study uses the bioecological model of human development and person-centered methods to describe the underlying patterns of risk and their association with bullying perpetration and victimization among U.S. children. MethodsUsing the National Survey of Children's Health, this study (n = 7319) explored the underlying patterns of risks across six domains (i.e., individual, family, school, neighborhood, economic, and socio-cultural) associated with bullying perpetration and victimization among U.S. elementary school children. ResultsLatent Class Analysis uncovered four patterns of risks. The low risks group (72.4%) showed the lowest rates of bullying perpetration (24.6%) and victimization (57.2%). The individual and environmental risks group (15.3%) presented moderate levels of bullying perpetration (31.8%) and victimization (67.1%). The family risks group (8.3%) showed moderate levels of bullying perpetration (35.9%) and victimization (66.0%). High risks group (4.0%) presented exceptionally high rates of bullying perpetration (59.1%) and victimization (87.3%). ConclusionResults suggest rates of bullying perpetration and victimization differed across the four patterns of risks. Understanding the sources of risk may be critical to alleviate bullying perpetration and victimization among children. ImplicationFindings suggest that child bullying should be approached with customized treatment considering their pattern of risk exposure.

A single-cell chromatin accessibility dataset of human primed and naïve pluripotent stem cell-derived teratoma

Teratoma, due to its remarkable ability to differentiate into multiple cell lineages, is a valuable model for studying human embryonic development. The similarity of the gene expression and chromatin accessibility patterns in these cells to those observed in vivo further underscores its potential as a research tool. Notably, teratomas derived from human naïve (pre-implantation epiblast-like) pluripotent stem cells (PSCs) have larger embryonic cell diversity and contain extraembryonic lineages, making them more suitable to study developmental processes. However, the cell type-specific epigenetic profiles of naïve PSC teratomas have not been yet characterized. Using single-cell assay for transposase-accessible chromatin sequencing (scATAC-seq), we analyzed 66,384 cell profiles from five teratomas derived from human naïve PSCs and their post-implantation epiblast-like (primed) counterparts. We observed 17 distinct cell types from both embryonic and extraembryonic lineages, resembling the corresponding cell types in human fetal tissues. Additionally, we identified key transcription factors specific to different cell types. Our dataset provides a resource for investigating gene regulatory programs in a relevant model of human embryonic development.

Understanding Left-Behind Places in a Contrastive Approach

Abstract How are left-behind places in a country compared to other types of places in an Eastern European country? A typology of local human development is designed and tested for the case of the Romanian society. The United Nations model of human development (structured by the three dimensions of education, economic development and health) is adapted to the local level and two contrasting measures are designed – an index and a typology of local human development. The typology resulted from a cluster analysis. It is validated by bi-variate and multivariate analysis (multinomial regression). High emigration rates, irrespective of the destination, do not bring lower development to the local origin in Romania: the destination of migration counts. There is a higher probability, in 2018, of left-behindness in localities with a higher number of emigrants to Italy in the years preceding 2011. Human development is highly differentiated by destination countries of emigration and by historical subregions. It is especially for such contexts that the quantitative approach could be misleading. Public policy targets could be better identified if qualitative and quantitative approaches are simultaneously used. The proposed approach could be adopted by keeping the same dimensions and measuring them by available and appropriate indicators.

Dynamic models of human development and concepts of the individual

Stem cells can be coaxed to self-organize into dynamic models of human development and early embryo formation. Despite their scientific promise, might widespread use of these technologies alter people's beliefs about what it means to be a human individual? Attention to some important philosophical distinctions may help navigate our thinking.

Biomechanical Analysis of Orbital Development: A Finite Element Analysis by an Experimentally Validated Model.

Constructing orbital finite element models capable of simulating the development process and analyzing the biomechanical mechanism. Four normal orbits from 1-month-old New Zealand white rabbits were used in this study. Toshiba Aquilion Prime was used to determine the computed tomography scan and direct orbital pressure manometry using an improved manometer based on the TSD104 pressure sensor transducer. The finite element analysis was conducted using the ANSYS Workbench platform. The biomechanics of each orbital wall improved to varying degrees as the rabbit orbit grew and developed. The von Mises stress in both rabbits initially concentrated at the lower edge of the posterior orbital wall, expanded to the entire orbit, and ultimately became more significant in the biomechanics of the region that consisted of the posterior orbital and superior orbital walls. During the expansion phase, the biomechanics of both rabbits gradually developed from the nasal side to the occipital side for radial displacement. It is evident that the finite element model is a good fit for simulating the physiological development of the rabbit orbit. The maximum radial displacement and maximum von Mises stress appeared 2 intermissions during the development of the orbit, at about 50 to 60 days and 80 to 90 days. This study establishes a theoretical foundation for the creation of a biomechanical model of human orbital development by offering the first finite element model to simulate orbital development and analyze the biomechanical mechanism of orbital pressure on orbital development.

HPSC-Derived Astrocytes at the Forefront of Translational Applications in Neurological Disorders.

Astrocytes, the most abundant glial cell type in the brain, play crucial roles in maintaining homeostasis within the central nervous system (CNS). Impairment or abnormalities of typical astrocyte functions in the CNS serve as a causative or contributing factor in numerous neurodevelopmental, neurodegenerative, and neuropsychiatric disorders. Currently, disease-modeling and drug-screening approaches, primarily focused on human astrocytes, rely on human pluripotent stem cell (hPSC)-derived astrocytes. However, it is important to acknowledge that these hPSC-derived astrocytes exhibit notable differences across studies and when compared to their in vivo counterparts. These differences may potentially compromise translational outcomes if not carefully accounted for. This review aims to explore state-of-the-art in vitro models of human astrocyte development, focusing on the developmental processes, functional maturity, and technical aspects of various hPSC-derived astrocyte differentiation protocols. Additionally, it summarizes their successful application in modeling neurological disorders. The discussion extends to recent advancements in the large-scale production of human astrocytes and their application in developing high-throughput assays conducive to therapeutic drug discovery.

From Divorce to Remarriage: Understanding the Experience of Remarried Women in a Collectivist Society

ABSTRACT This study explores the subjective experiences of remarriage among five divorced Malay Muslim women with children from their first marriages, who have since remarried. Using semi-structured interviews, their remarriage journeys were analyzed through Interpretative Phenomenological Analysis. Four main themes – internal process, adjusting to a new marriage, relationships with others, and marital satisfaction – along with ten sub-themes were identified. These themes encompass emotional struggles post-divorce, acceptance of reality and fate, mixed feelings pre-remarriage, decision-making in remarriage, dynamics within couple roles and interactions, and relationships with children, in-laws, extended family, and ex-spouses, culminating in the joy and gratitude experienced in their current marriages. This study was informed by Carter & McGoldrick’s Remarried Family Formation Framework and Bronfenbrenner’s Bioecological Model of Human Development, which together provided a robust theoretical lens for understanding the complexities of remarriage in a culturally rich setting. The findings offer significant implications for practitioners working with remarried individuals and highlight areas for future research.

Using Trentham’s principle of inverse consistency to vet models of human development for use in Christian educator preparation programs

This article aims to utilize Trentham’s Inverse Consistency Protocol (ICP) to appraise human development models for use within Christian educator preparation programs. The authors propose that the ICP can be applied to any secular paradigms to discern potential areas of inconsistency with an institution’s faith tradition. To illustrate application of the ICP in this way, Vygotsky’s Zone of Proximal Development (ZPD) model was provided as an example of one model of human development that is widely taught in educator preparation programs. The ZPD model was submitted through the four-step inverse consistency protocol. Findings suggest that ICP is a reflective tool for engaging with the social sciences with integrity after protocol results indicated that ZPD is an appropriate model for use by Christian educators when used responsibly. Implications for extended use of ICP are discussed.

Primitive macrophages induce sarcomeric maturation and functional enhancement of developing human cardiac microtissues via efferocytic pathways.

Yolk sac macrophages are the first to seed the developing heart, however we have no understanding of their roles in human heart development and function due to a lack of accessible tissue. Here, we bridge this gap by differentiating human embryonic stem cells (hESCs) into primitive LYVE1+ macrophages (hESC-macrophages) that stably engraft within contractile cardiac microtissues composed of hESC-cardiomyocytes and fibroblasts. Engraftment induces a human fetal cardiac macrophage gene program enriched in efferocytic pathways. Functionally, hESC-macrophages trigger cardiomyocyte sarcomeric protein maturation, enhance contractile force and improve relaxation kinetics. Mechanistically, hESC-macrophages engage in phosphatidylserine dependent ingestion of apoptotic cardiomyocyte cargo, which reduces microtissue stress, leading hESC-cardiomyocytes to more closely resemble early human fetal ventricular cardiomyocytes, both transcriptionally and metabolically. Inhibiting hESC-macrophage efferocytosis impairs sarcomeric protein maturation and reduces cardiac microtissue function. Taken together, macrophage-engineered human cardiac microtissues represent a considerably improved model for human heart development, and reveal a major beneficial role for human primitive macrophages in enhancing early cardiac tissue function.

The limitations of automatically generated curricula for continual learning.

In many applications, artificial neural networks are best trained for a task by following a curriculum, in which simpler concepts are learned before more complex ones. This curriculum can be hand-crafted by the engineer or optimised like other hyperparameters, by evaluating many curricula. However, this is computationally intensive and the hyperparameters are unlikely to generalise to new datasets. An attractive alternative, demonstrated in influential prior works, is that the network could choose its own curriculum by monitoring its learning. This would be particularly beneficial for continual learning, in which the network must learn from an environment that is changing over time, relevant both to practical applications and in the modelling of human development. In this paper we test the generality of this approach using a proof-of-principle model, training a network on two sequential tasks under static and continual conditions, and investigating both the benefits of a curriculum and the handicap induced by continuous learning. Additionally, we test a variety of prior task-switching metrics, and find that in some cases even in this simple scenario the a network is often unable to choose the optimal curriculum, as the benefits are sometimes only apparent with hindsight, at the end of training. We discuss the implications of the results for network engineering and models of human development.

The Virtual Child.

We are building the world's first Virtual Child-a computer model of normal and cancerous human development at the level of each individual cell. The Virtual Child will "develop cancer" that we will subject to unlimited virtual clinical trials that pinpoint, predict, and prioritize potential new treatments, bringing forward the day when no child dies of cancer, giving each one the opportunity to lead a full and healthy life.