Human Deaths Research Articles

A Statistical Review of the Impact of COVID-19 on the World

The COVID-19 epidemic has caused an unparalleled era of disruption, significantly impacting public health, the environment, the economy, and civilizations globally. This data analysis offers a thorough examination of COVID-19's worldwide effects. Through statistical methods, it explores the epidemic's spread, mechanics of transmission, and social repercussions. In addition, it carries out quantitative research to comprehend how the pandemic affects society's behavior, healthcare systems, and the environment. To understand the substantial repercussions of the pandemic, the review combines many datasets and presents data from a reliable source. Through the analysis of both quantitative and qualitative data, this review focuses on the pandemic's effects from several perspectives, such as public health measurements, societal shifts, and environmental changes. The findings demonstrate the horrifying cost of human deaths and describe how society has adapted to new issues and routines. The study additionally examines the data's graphical and tabular format, which comprises the total number of COVID-19 cases and fatalities that have been confirmed in nine different nations.

Evaluating approaches for reducing catastrophic risks from AI

AbstractAccording to a growing number of researchers, AI may pose catastrophic – or even existential – risks to humanity. Catastrophic risks may be taken to be risks of 100 million human deaths, or a similarly bad outcome. I argue that such risks – while contested – are sufficiently likely to demand rigorous discussion of potential societal responses. Subsequently, I propose four desiderata for approaches to the reduction of catastrophic risks from AI. The quality of such approaches can be assessed by their chance of success, degree of beneficence, degree of non-maleficence, and beneficent side effects. Then, I employ these desiderata to evaluate the promises, limitations and risks of alignment research, timelines research, policy research, halting or slowing down AI research, and compute governance for tackling catastrophic AI risks. While more research is needed, this investigation shows that several approaches for dealing with catastrophic AI risks are available, and where their respective strengths and weaknesses lie. It turns out that many approaches are complementary and that the approaches have a nuanced relationship to approaches to present AI harms. While some approaches are similarly useful for addressing catastrophic risks and present harms, this is not always the case.

Moringa oleifera Leaf Extract and (Amorphophallus muelleri Blume.) Glucomannan Effects on Obese White Rat Feed Intake and Body Weight

Obesity significantly raises the rates of illness and death in humans due to its association with several health conditions such as cardiovascular disease, hypertension, hypercholesterolemia, type 2 diabetes mellitus, osteoporosis, depression, breast cancer, and colon cancer. This study aims to determine the effect of Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) on feed intake and body weight of obese white rats. This study used an experimental method with a pretest-posttest-only control grub design using 24 white male rats of the Wistar strain aged 8 weeks which were divided into 2 control groups, namely positive(K1) and negative(K2) and six treatment groups with extract doses Moringa oleifera leaves and glucomannan (Amorphophallus muelleri Blume.) 100mg/kgBW:100mg/kgBW(K3), 80mg/kgBW:120mg/kgBW(K4), 120mg/kgBW: 80mg/kgBW(K5), 50mg/kgBW:50mg/kgBW(K6), 40mg/kgBW:60mg/kgBW(K7), and 60mg/kgBW:40mg/kgBW(K8). Data analysis on body weight and feed intake used the SPSS ANOVA test and paired T-test. The results of the paired T-test (p<0.05) showed that Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) significantly reduced body weight and feed intake. In conclusion, administration of Moringa oleifera leaf extract and glucomannan (Amorphophallus muelleri Blume.) in group K4 (80 mg/kgBW Moringa oleifera leaf extract: 120 mg/kgBW glucomannan Amorphophallus muelleri Blume) can reduce feed intake (4.29 g) and body weight (36.67 g) effectively.

IJCM_219A: A “One Health” Model for Prevention and Control of Anthrax in Koraput, Odisha, India

Background: Anthrax, a neglected zoonotic disease caused by Bacillus anthracis, poses a significant threat in Odisha, India, particularly in the tribal district of Koraput, which has reported numerous human cases and deaths but lacks consistent robust surveillance. Objectives: This study aimed to pilot a “One Health” surveillance model in Koraput, involving community health workers, para-veterinarians, and block- level teams in outbreak investigations. Methodology: As part of the “One Health Approach to Anthrax Control in India (OHAI)” project Odisha University of Agriculture and Technology (OUAT), in collaboration with the Departments of Animal Husbandry and Health and Family Welfare, Government of Odisha, initiated prospective laboratory-based anthrax surveillance in three blocks of Koraput. Confirmed cases in animals were identified via microscopic, culture, or PCR testing for Bacillus anthracis. Community awareness programs and rapport-building exercises were conducted with human and veterinary officials at the block and district levels following the detection of outbreaks. Results: Between March and June 2023, five anthrax outbreaks were detected in Koraput, with four involving both cutaneous human and animal deaths and one in animals only. Livestock losses included cattle (27), goats (2), and buffalo (1). Of the 38 samples collected (blood, bone, tissue, and soil), 11 (35%) tested positive for anthrax by RT-PCR. OUAT rapidly communicated results (<24 hours) to the state health department, leading to joint investigations and control measures, including livestock vaccination, safe carcass disposal, and medical outreach camps for screening and prophylaxis. In total, five district/block level meetings and two state-level consultations were held, culminating in a zoonotic roadmap draft, which included an anthrax prevention and control action plan. Conclusion: The study’s adoption of the One Health framework facilitated a rapid response to anthrax outbreaks in Koraput, effectively managing both human and animal cases. This model presents a viable approach for controlling anthrax in endemic areas of India.

Copper selenide nanosheets with photothermal therapy-related properties and multienzyme activity for highly effective eradication of drug resistance

Bacterial infections are among the most significant causes of death in humans. Chronic misuse or uncontrolled use of antibiotics promotes the emergence of multidrug-resistant superbugs that threaten public health through the food chain and cause environmental pollution. Based on the above considerations, copper selenide nanosheets (CuSe NSs) with photothermal therapy (PTT)- and photodynamic therapy (PDT)-related properties have been fabricated. These CuSe NSs possess enhanced PDT-related properties and can convert O2 into highly toxic reactive oxygen species (ROS), which can cause significant oxidative stress and damage to bacteria. In addition, CuSe NSs can efficiently consume glutathione (GSH) at bacterial infection sites, thus further enhancing their sterilization efficacy. In vitro antibacterial experiments with near-infrared (NIR) irradiation have shown that CuSe NSs have excellent photothermal bactericidal properties. These experiments also showed that CuSe NSs exerted excellent bactericidal effects on wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) and significantly promoted the healing of infected wounds. Because of their superior biological safety, CuSe NSs are novel copper-based antimicrobial agents that are expected to enter clinical trials, serving as a modern approach to the major problem of treating bacterially infected wounds.

Empowering rural Maharashtra: A holistic approach to rabies control and one health perspectiv

Rabies, a grave threat in rural areas with limited healthcare access, poses significant global concern due to its near 100% fatality rate in humans and animals. The shift to intradermal vaccines highlights cost-effectiveness. Maharashtra, India’s one of largest states, faces unique challenges in rabies prevention and management, despite efforts, owing to poor awareness and limited healthcare access. In India, Maharashtra stands out due to challenges in rabies prevention and management despite ongoing efforts. This study proposes transformative strategies grounded in community engagement, education and vaccination campaigns to reduce rabies incidence. Emphasizing community empowerment, it advocates for integrating traditional knowledge with modern interventions. Globally, 330,000 people face rabies risk annually, with India accounting for 36% of cases. Limited vaccine access perpetuates transmission risks, necessitating collaborative efforts to enhance awareness and intervention, particularly in rural Maharashtra. The study emphasizes removing stray dogs and implementing widespread vaccination as key prevention strategies. The success of Goa’s One Health program exemplifies human rabies elimination feasibility and cost-effectiveness, aiming to eradicate canine-mediated human rabies deaths by 2030. Targeted interventions can effectively reduce rabies incidence, safeguarding both human and animal populations. By targeting these key areas, communities can effectively reduce the incidence of rabies and protect both human and animal population.

The impact of the Russia-Ukraine war on crime dynamics in the Republic of Poland in terms of ukrainian refugeeism

ObjectivesThe objectiv of the study was to answer the question: what impact has the wave of refugees from Ukraine caused by the outbreak of war on its territory had on the dynamics of crime in Poland?Material and methodsThe arrivals from Ukraine in the Republic of Poland and departures for Ukraine were analyzed. The study also examines the population dynamics in Poland from 2018 to 2023, including deaths and births. The research also takes into account the impact of the Covid-19 pandemic on the population size in Poland. Such an approach was necessary to conduct a comparative analysis of changes in population dynamics and crime dynamics in Poland in order to determine the relationship between them in the examined period, taking into account national demographic changes. At the same time, crimes detected in Poland from 2019 to 2023 in the same periods, from January to October, were analyzed.ResultsStudies show that the COVID-19 pandemic and migration from Ukraine to Poland had a noticeable impact on the population in the Republic of Poland. In 2021, the population in Poland decreased, which was mainly due to the impact of the COVID-19 pandemic and a large increase in the number of deaths. In 2022, compared to 2021, there was an increase in the population as a result of a decrease in human deaths, as well as the granting of Polish citizenship to foreigners in 2022. At the same time, the analysis looked at the numbers of crimes found in Poland in 2019-2023 in univariate time frames from January to October combined, which showed no significant changes in this regard.ConclusionsThe results from the research allowed to conclude that the wave of refugees from Ukraine caused by the outbreak of war on its territory did not noticeably affect the increase in crime in Poland.

Impaired autophagy following ex vivo cooling of simulated hypothermic temperatures in peripheral blood mononuclear cells from young and older adults

Hypothermia is a critical consequence of extreme cold exposure that increases the risk of cold-related injury and death in humans. While the initiation of cytoprotective mechanisms including the process of autophagy and the heat shock response (HSR) is crucial to cellular survival during periods of stress, age-related decrements in these systems may underlie cold-induced cellular vulnerability in older adults. Moreover, whether potential sex-related differences in autophagic regulation influence the human cold stress response remain unknown. We evaluated the effect of age and sex on mechanisms of cytoprotection (autophagy and the HSR) and cellular stress (apoptotic signaling and the acute inflammatory response) during ex vivo hypothermic cooling. Venous blood samples from 20 healthy young (10 females; mean [SD]: 22 [2] years) and 20 healthy older (10 females; 66 [5] years) adults were either isolated immediately (baseline) for peripheral blood mononuclear cells (PBMCs) or exposed to water bath temperatures maintained at 37, 35, 33, 31, or 4 °C for 90 min before PBMC isolation. Proteins associated with autophagy, apoptosis, the HSR, and inflammation were analyzed via Western blotting. Indicators of autophagic initiation and signaling (LC3, ULK1, and beclin-2) and the HSR (HSP90 and HSP70) increased when exposed to hypothermic temperatures in young and older adults (all p ≤ 0.007). Sex-related differences were only observed with autophagic initiation (ULK1; p = 0.015). However, despite increases in autophagic initiators ULK1 and beclin-2 (all p < 0.001), this was paralleled by autophagic dysfunction (increased p62) in all groups (all p < 0.001). Further, apoptotic (cleaved-caspase-3) and inflammatory (IL-6 and TNF-α) signaling increased in all groups (all p < 0.001). We demonstrated that exposure to hypothermic conditions is associated with autophagic dysfunction, irrespective of age or sex, although there may exist innate sex-related differences in cytoprotection in response to cold exposure as evidenced through altered autophagic initiation.

BRAIN TUMOR DETECTION USING DEEP TRANSFER LEARNING

One of the leading causes of cancer-related death in humans is brain tumors. The process of diagnosing brain tumors and helping patients choose their course of treatment depends heavily on early identification. An innovative medical imaging method called Magnetic Reasoning Imaging (MRI) aids radiologists in locating the tumor spot. Manually testing the MRI pictures is a laborious process that needs experience. The development of computer-assisted diagnosis, machine learning, and deep learning in particular has made it possible for radiologists to more accurately diagnose brain tumors these days. The project employs Convolutional Neural Networks (CNNs) the classification of MRI images targeting four distinct types of brain tumors: gliomas, meningiomas, pituitary adenomas, and no tumor. By harnessing the power of deep learning, the model aims to enhance the precision and speed of tumor identification, contributing to early diagnosis and improved patient outcomes. But the traditional CNN has limitations, such as the need for large labeled datasets, employment of more layer of neurons, need for large computational resources, more time consumption and the risk of overfitting. To overcome these challenges, the project leverages transfer learning, a technique that allows the model to leverage knowledge gained from pre-trained networks on large datasets. This project involves the use of EfficientNetB0 architecture for the detection of brain tumor. This methodology demonstrates superior classification performance compared to conventional Neural Network. Therefore, this project presents a robust framework for brain tumor detection utilizing deep transfer learning, offering a promising avenue for improving diagnostic accuracy in the realm of neuroimaging. The adoption of transfer learning mitigates the drawbacks associated with CNNs, propelling the development of effective and reliable method for early detection and intervention in brain tumor cases. Key Words: MRI, CNN, Brain tumor, Transfer Learning, EffiecientNetB0.

Eliminating invisible deaths: the woeful state of global rabies data and its impact on progress towards 2030 sustainable development goals for neglected tropical diseases

Like other neglected diseases, surveillance data for rabies is insufficient and incompatible with the need to accurately describe the burden of disease. Multiple modeling studies central to estimating global human rabies deaths have been conducted in the last two decades, with results ranging from 14,000 to 74,000 deaths annually. Yet, uncertainty in model parameters, inconsistency in modeling approaches, and discrepancies in data quality per country included in global burden studies have led to recent skepticism about the magnitude of rabies mortality. Lack of data not only limits the efficiency and monitoring of rabies elimination strategies but also severely diminishes abilities to advocate for support from international funding agencies. Meanwhile, the most vulnerable communities continue to suffer from deaths that could have been prevented through more robust reporting. The Zero by 30 global strategy to eliminate dog-mediated human rabies by 2030 recommends endemic countries adopt the intersectoral approach, Integrated Bite Case Management (IBCM), as a cost-effective method to enhance surveillance. However, effective implementation of IBCM is impeded by challenges such as limited capacity, resources, knowledge, skills, and attitudes toward compliance. To address this, the World Health Organization and United Against Rabies Forum have developed several open-access tools to guide national control programs in strong data collection practices, and online data repositories to pragmatically streamline reporting and encourage data sharing. Here, we discuss how current and future initiatives can be best employed to improve the implementation of existing surveillance tools and prioritization of effective data reporting/sharing to optimize progress toward 2030 elimination.

Rabies incidence and burden in three cities of Cameroon (2004–2013)

Rabies is a fatal disease occurring worldwide and especially in almost all the countries in Asia and Africa including Cameroon. Though animal and human rabies is prevalent in Cameroon, the epidemiology and socio-economic burden of the disease in the country is not known. Therefore, a 10-year (October 2004–April 2013) retrospective study on the incidence of animal and human rabies and its burden in Garoua, Ngaoundéré and Yaoundé in Cameroon was carried out. Records of human cases were extracted from the database of the regional hospitals, and animal cases from the databases of Centre Pasteur and National Veterinary Laboratory. The burden of the disease was assessed through the estimation of costs linked to preventive measures (vaccination), corrective procedures (Post Exposure Treatment), Disability-Adjusted Life Year (DALY) and overall societal cost of the disease. Overall, 56 rabies-suspected human deaths, corresponding to an incidence of 0.02 ± 0.00 ‱ and Animal Rabies Incidence (ARI) of 0.37 ± 0.00 % among 1844 suspected animal cases were recorded. The economic loss due to preventive measures of 326,046 ± 28,130.85 USD, related to corrective procedures of 806,741.25 ± 2,466.08 USD, and DALY of 1690.28 ± 4.76 years were estimated. This is the first study that highlights the enormous socio-economic burden associated with animal and human rabies in endemic parts of Cameroon and emphasizes on enhancing rabies eradication strategy focusing on the One Health approach.

Investigating Rabies Prevention and Control: A Study of Knowledge, Attitudes, and Practices in Jimma Town, South-Western Ethiopia

Introduction: Ethiopia has the second highest number of human rabies deaths among African countries. However, the lack of public awareness regarding rabies prevention and control is a major obstacle in combating this disease. To address this issue, the current study was conducted in Jimma Town, southwestern Ethiopia, to assess the knowledge, attitude, and practice (KAP) of households regarding rabies and its associated factors. Materials and methods: The current community-based cross-sectional study was carried out, involving a total of 421 participants. The KAP of the respondents was evaluated using principal component analysis and ranked accordingly. Results: Out of the 421 study participants, 53% were female and 63.4% were male. Among the participants, 62.3% demonstrated an adequate level of knowledge, 51% exhibited a positive attitude, and 43% practiced effective prevention and control measures against rabies. On the other hand, younger and male participants were found to have positive attitudes towards rabies. The study revealed that individuals with lower levels of education, specifically those who had not completed college as well as the unemployed and those without dogs had poor knowledge attitudes, and practices about rabies. Conclusion: These findings highlight the need to improve the understanding and awareness of rabies within the community.

LVS ΔcapB-vectored multiantigenic melioidosis vaccines protect against lethal respiratory Burkholderia pseudomallei challenge in highly sensitive BALB/c mice.

Melioidosis, a major neglected disease caused by the intracellular bacterial pathogen Burkholderia pseudomallei, is endemic in many tropical areas of the world and causes an estimated 165,000 cases and 89,000 deaths in humans annually. Moreover, B. pseudomallei is categorized as a Tier 1 select agent of bioterrorism, largely because inhalation of low doses can cause rapidly fatal pneumonia. No licensed vaccine is available to prevent melioidosis. Here, we describe a safe and potent melioidosis vaccine that protects against lethal respiratory challenge with B. pseudomallei in a highly sensitive small animal model-even a single immunization is highly protective, and the vaccine gives long-term protection. The vaccine utilizes a highly attenuated replicating intracellular bacterium as a vector to express multiple key proteins of B. pseudomallei; this vector platform has previously been used successfully to develop potent vaccines against other Tier 1 select agent diseases including tularemia, anthrax, and plague.

Comparative analysis of rabies pathogenic and vaccine strains detection by RIG-I-like receptors

Rabies virus (RABV) is a lethal neurotropic virus that causes 60,000 human deaths every year globally. RABV infection is characterized by the suppression of the interferon (IFN)-mediated antiviral response. However, molecular mechanisms leading to RABV sensing by RIG-I-like receptors (RLR) that initiates IFN signaling currently remain elusive. Here, we showed that RABV RNAs are primarily recognized by the RIG-I RLR, resulting in an IFN response in the infected cells, but this response varied according to the type of RABV used. Pathogenic RABV strain RNAs, Tha, were poorly detected in the cytosol by RIG-I and therefore caused a weak antiviral response. However, we revealed a strong IFN activity triggered by the attenuated RABV vaccine strain RNAs, SAD, mediated by RIG-I. We characterized two major 5′ copy-back defective interfering (5′cb DI) genomes generated during SAD replication. Furthermore, we identified an interaction between 5′cb DI genomes, and RIG-I correlated with a high stimulation of the type I IFN signaling. This study indicates that wild-type RABV RNAs poorly activate the RIG-I pathway, while the presence of 5′cb DIs in the live-attenuated vaccine strain serves as an intrinsic adjuvant that strengthens its efficiency by enhancing RIG-I detection thus strongly stimulates the IFN response.

Statistical modeling of factors associated with human deaths per road traffic accident of Jimma town, Ethiopia

Statistical modeling of factors associated with human deaths per road traffic accident of Jimma town, Ethiopia

PDX proteins from Arabidopsis thaliana as novel substrates of cathepsin B: implications for vitamin B6 biosynthesis regulation.

Vitamin B6 is a critical molecule for metabolism, development, and stress sensitivity in plants. It is a cofactor for numerous biochemical reactions, can serve as an antioxidant, and has the potential to increase tolerance against both biotic and abiotic stressors. Due to the importance of vitamin B6, its biosynthesis is likely tightly regulated. Plants can synthesize vitamin B6 de novo via the concerted activity of Pyridoxine Biosynthesis Protein 1 (PDX1) and PDX2. Previously, PDX proteins have been identified as targets for ubiquitination, indicating they could be marked for degradation by two highly conserved pathways: the Ubiquitin Proteasome Pathway (UPP) and the autophagy pathway. Initial experiments show that PDXs are in fact degraded, but surprisingly, in a ubiquitin-independent manner. Inhibitor studies pointed toward cathepsin B, a conserved lysosomal cysteine protease, which is implicated in both programed cell death and autophagy in humans and plants. In plants, cathepsin Bs are poorly described, and no confirmed substrates have been identified. Here, we present PDX proteins from Arabidopsis thaliana as interactors and substrates of a plant Cathepsin B. These findings not only describe a novel cathepsin B substrate in plants, but also provide new insights into how plants regulate de novo biosynthesis of vitamin B6.

Evidence for a host switching in the maintenance of canid rabies variant in two wild carnivore species in the Northern Cape Province, South Africa.

Rabies is a zoonotic infectious disease that causes at least 59 000 human deaths worldwide annually, with 95% of the cases occurring in the developing countries of Asia and Africa. There are two Lyssavirus rabies (RABV) variants circulating in South Africa, notably the canid and mongoose RABV biotypes. The canid RABV biotype is maintained in the domestic dog and two wild carnivore species, the black-backed jackal (Canis mesomelas) and the bat-eared fox (Otocyon megalotis). The yellow mongoose, a member of the Herpestidae family, is a reservoir and vector species for the mongoose RABV biotype. Rabies trends showed an increase in rabiespositive cases in aardwolves between 2011 and 2016 surpassing the bat-eared fox as the most rabies-affected wild carnivore in the Northern Cape Province of South Africa. The aim of the study was to establish the genetic relationships amongst rabies viruses recovered from both the aardwolves and bat-eared foxes. A partial region of the glycoprotein gene and the variable G-L intergenic region of the viral genome were analysed using nucleotide sequences generated from PCR amplicons. The rabies viruses recovered from the aardwolves between the year 2015 and 2017 were 100% nucleotide sequence identical, suggesting a single or common source and possible evidence for a host shift. Furthermore, the phylogenetic reconstruction demonstrated that the rabies viruses obtained from the two wild carnivore species from the Northern Cape Province clustered independently of each o ther with 96% nucleotide sequence identity, suggesting that the aardwolf may be able to maintain the canid RABV variant in this geographical area.

An analysis of the implementation of COVID-19 handling and control governments in Indonesia

The novel coronavirus has caused 6.85 million human deaths worldwide. The World Health Organization (WHO) says the coronavirus is here to stay, despite the declining trend of cases, but serious efforts are needed from all elements of society to end this global health crisis. Indonesia is one of the countries that is very consistent in suppressing the spread of the virus, one of the breakthroughs made is activating local government tools for prevention and serious handling. The purpose of this paper is to provide an overview of the Coronavirus prevention and handling model carried out by local governments throughout Indonesia. The results of this study prove that as many as 38 provinces with 415 districts in Indonesia have not been able to maximally implement the coronavirus prevention and handling model following the framework formulated by the Indonesian Ministry of Health, this is due to many factors, including; the work culture of the state apparatus, weak middle-level management, as well as weak monitoring and evaluation issues.

Problematika Penegakan Hukum Kasus Pembunuhan Berencana di Indonesia: Studi Kasus Pembunuhan Berencana Kopi Sianida oleh Jessica Wongso terhadap Mirna Salihin

This research was conducted to look at the problems of law enforcement in Indonesia in the case of the premeditated murder of Wayan Mirna Salihin. Whether the judge's decision has provided justice that is as fair as possible to the victim and perpetrator, the evidence presented at the trial does not comply with procedures in accordance with the Criminal Code, the emergence of the films Ice Cold Murder, Coffee and Jessica Wongso has made the public less confident in the law in Indonesia. It is necessary to examine the role of the forensic autopsy in uncovering this case, the discovery of cyanide in Mirna's stomach, which is very strong evidence to prove that the cause of Mirna's death was poisoning by her friend Jessica Wongso. Research methods used used in this research is normative legal research, research using a problem approach, namely judicial case study by approaching the Mirna Salihin premeditated murder case, by examining the judge's decision which resulted in Jessica Wongso being convicted, by conducting this research uncovering the irregularities that occurred in This murder case creates a conflict of interest that cannot be resolved. Jessica Kumala Wongso has been convicted of violating Article 340 of the Criminal Code, namely premeditated murder. In the results of the examination, experts said that a complete autopsy must be carried out for human deaths due to poison. The expert concluded that the cause of WAYAN MIRNA SALIHIN's death was cyanide, especially since 0.2 mg/liter of cyanide was found in the stomach. The judge's decision regarding the premeditated murder of Mirna Salihin did not have any valid and strong evidence at all, starting from the testimony of witnesses who did not there was someone who saw Jessica put poison in the coffee that Mirna was drinking, then the expert's testimony in the examination was not perfect, one of which was that the autopsy did not comply with proper procedures, as well as evidence, such as CCTV which was not original and contrary to regulations. KAPOLRI No. 10 of 2009, as well as the defendant's statement that it never occurred to him to kill his own friend.

The Influenza A Virus Replication Cycle: A Comprehensive Review.

Influenza A virus (IAV) is the primary causative agent of influenza, colloquially called the flu. Each year, it infects up to a billion people, resulting in hundreds of thousands of human deaths, and causes devastating avian outbreaks with worldwide losses worth billions of dollars. Always present is the possibility that a highly pathogenic novel subtype capable of direct human-to-human transmission will spill over into humans, causing a pandemic as devastating if not more so than the 1918 influenza pandemic. While antiviral drugs for influenza do exist, they target very few aspects of IAV replication and risk becoming obsolete due to antiviral resistance. Antivirals targeting other areas of IAV replication are needed to overcome this resistance and combat the yearly epidemics, which exact a serious toll worldwide. This review aims to summarise the key steps in the IAV replication cycle, along with highlighting areas of research that need more focus.